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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4080R-2 |
| Symbol |
CG4080 |
Full Name |
CG4080 |
| CG No |
CG4080 |
Old CG No |
CG4080 |
| Synonyms |
CG4080 |
| Accession No (Link to NCBI) |
NM_140048.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Fan W, Lam SM, Xin J, Yang X, Liu Z, Liu Y, Wang Y, Shui G, Huang X.
Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition.
PLoS Genet (2017) 13(3) e1006664
[
PubMed ID = 28273089
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCTCCGAGACGAACTCCTGCGA |
| Primer Seq. 3' |
aatctagaggtaccCACAGTATCCTCCGGCTGCAC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CTCCGAGACG AACTCCTGCG AGTTGTGCAA GTTCCCCTTC ATCATGCACA CCAAGATCAA
0061 ACCCTTCAAC GAGTGGCGCA GCCTTGACAT CTCCGGCATT GAAAGGCGGC GGCTGTGCTG
0121 CTCCGTGCTG TTCCATTGCG CCGCAGCCCT GTGCGTCATC TGGTCCCTGT GTGTGCTCAT
0181 CGAGNGGGCC GCCGACGATG TCCAGCGTGG CCTAATAGAC TGGCCCTTCT GGACAAAGCT
0241 GGCCGTTGTC ACCGTGGGCC TTACCGGCGG AATAGTTTTT ATGTATATCC AGTGCAAGGC
0301 ATATCTTCAC CTCTGTCACC GTTGGAAAGC ACGCAACAGA ATTCTACTGA TTCAGAATGC
0361 TCCAGAAAAG ATACATCCAG TGGCACCTCC ATCCCCAGTG GCCGCCCATC ATCAGCACTT
0421 CAGTGAGCCA CTGGCTCATG CCGGATCGAC GAGCGGAGCA GTGGAGATCA ATGCCAgNGG
0481 TGCAGCCGGA GGATACTGTG |
| in silico PCR Fragment |
0001 CTCCGAGACG AACTCCTGCG AGTTGTGCAA GTTCCCCTTC ATCATGCACA CCAAGATCAA
0061 ACCCTTCAAC GAGTGGCGCA GCCTTGACAT CTCCGGCATT GAAAGGCGGC GGCTGTGCTA
0121 CTCCGTGCTG TTCCATTGCG CCGCAGCCCT GTGCGTCATC TGGTCCCTGT GTGTGCTCAT
0181 CGAGCGGGCC GCCGACGATG TCCAGCGTGG CCTAATAGAC TGGCCCTTCT GGACAAAGCT
0241 GGCCGTTGTC ACCGTGGGCC TTACCGGCGG AATAGTTTTT ATGTATATCC AGTGCAAGGC
0301 ATATCTTCAC CTCTGTCACC GTTGGAAAGC ACGCAACAGA ATTCTACTGA TTCAGAATGC
0361 TCCAGAAAAG ATACATCCAG TGGCACCTCC ATCCCCAGTG GCCGCCCATC ATCAGCACTT
0421 CAGTGAGCCA CTGGCTCATG CCGGATCGAC GAGCGGAGCA GTGGAGATCA ATGCCAGCGG
0481 TGCAGCCGGA GGATACTGTG |
| Assemble Data |
4080R-2.IR_full 1 CTCCGAGACGAACTCCTGCGAGTTGTGCAAGTTCCCCTTCATCATGCACACCAAGATCAA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4080R-2.in silico 1 CTCCGAGACGAACTCCTGCGAGTTGTGCAAGTTCCCCTTCATCATGCACACCAAGATCAA 60
4080R-2.IR_full 61 ACCCTTCAACGAGTGGCGCAGCCTTGACATCTCCGGCATTGAAAGGCGGCGGCTGTGCTG 120
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4080R-2.in silico 61 ACCCTTCAACGAGTGGCGCAGCCTTGACATCTCCGGCATTGAAAGGCGGCGGCTGTGCTA 120
4080R-2.IR_full 121 CTCCGTGCTGTTCCATTGCGCCGCAGCCCTGTGCGTCATCTGGTCCCTGTGTGTGCTCAT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4080R-2.in silico 121 CTCCGTGCTGTTCCATTGCGCCGCAGCCCTGTGCGTCATCTGGTCCCTGTGTGTGCTCAT 180
4080R-2.IR_full 181 CGAGNGGGCCGCCGACGATGTCCAGCGTGGCCTAATAGACTGGCCCTTCTGGACAAAGCT 240
|||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
4080R-2.in silico 181 CGAGCGGGCCGCCGACGATGTCCAGCGTGGCCTAATAGACTGGCCCTTCTGGACAAAGCT 240
4080R-2.IR_full 241 GGCCGTTGTCACCGTGGGCCTTACCGGCGGAATAGTTTTTATGTATATCCAGTGCAAGGC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4080R-2.in silico 241 GGCCGTTGTCACCGTGGGCCTTACCGGCGGAATAGTTTTTATGTATATCCAGTGCAAGGC 300
4080R-2.IR_full 301 ATATCTTCACCTCTGTCACCGTTGGAAAGCACGCAACAGAATTCTACTGATTCAGAATGC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4080R-2.in silico 301 ATATCTTCACCTCTGTCACCGTTGGAAAGCACGCAACAGAATTCTACTGATTCAGAATGC 360
4080R-2.IR_full 361 TCCAGAAAAGATACATCCAGTGGCACCTCCATCCCCAGTGGCCGCCCATCATCAGCACTT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4080R-2.in silico 361 TCCAGAAAAGATACATCCAGTGGCACCTCCATCCCCAGTGGCCGCCCATCATCAGCACTT 420
4080R-2.IR_full 421 CAGTGAGCCACTGGCTCATGCCGGATCGACGAGCGGAGCAGTGGAGATCAATGCCAGNGG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
4080R-2.in silico 421 CAGTGAGCCACTGGCTCATGCCGGATCGACGAGCGGAGCAGTGGAGATCAATGCCAGCGG 480
4080R-2.IR_full 481 TGCAGCCGGAGGATACTGTG 500
||||||||||||||||||||
4080R-2.in silico 481 TGCAGCCGGAGGATACTGTG 500
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_140048.2 |
CG4080-RA (CG4080), mRNA |
| 0 |
0 |
3 |
5 |
6 |
NM_143795.3 |
CG3920-RA, transcript variant A (l(2)k16918), mRNA |
| 0 |
0 |
3 |
5 |
6 |
NM_164561.2 |
CG3920-RB, transcript variant B (l(2)k16918), mRNA |
| 0 |
0 |
3 |
4 |
6 |
NM_132540.1 |
CG2467-RA, transcript variant A (CG2467), mRNA |
| 0 |
0 |
3 |
4 |
6 |
NM_167313.1 |
CG2467-RB, transcript variant B (CG2467), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_169177.1 |
CG2507-RB, transcript variant B (sas), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_057263.2 |
CG2507-RA, transcript variant A (sas), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_176465.1 |
CG31211-RC, transcript variant C (CG31211), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_141874.2 |
CG31211-RA, transcript variant A (CG31211), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_176464.1 |
CG31211-RB, transcript variant B (CG31211), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_080306.2 |
CG3443-RB (pcx), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_079174.2 |
CG11495-RA (rasp), mRNA |
| 0 |
0 |
1 |
6 |
8 |
NM_206253.1 |
CG12002-RE, transcript variant E (Pxn), mRNA |
| 0 |
0 |
1 |
6 |
8 |
NM_206254.1 |
CG12002-RD, transcript variant D (Pxn), mRNA |
| 0 |
0 |
1 |
6 |
8 |
NM_206255.1 |
CG12002-RC, transcript variant C (Pxn), mRNA |
| 0 |
0 |
1 |
6 |
8 |
NM_079167.3 |
CG12002-RA, transcript variant A (Pxn), mRNA |
| 0 |
0 |
1 |
6 |
8 |
NM_167957.1 |
CG12002-RB, transcript variant B (Pxn), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_001015216.1 |
CG40156-PA.3 (CG40156), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_165540.2 |
CG2140-RA, transcript variant A (Cyt-b5), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_136450.2 |
CG2140-RB, transcript variant B (Cyt-b5), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_078601.2 |
CG9533-RA (rut), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_057383.5 |
CG9218-RA, transcript variant A (sm), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_166368.3 |
CG9218-RC, transcript variant C (sm), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001032269.1 |
CG9218-RE, transcript variant E (sm), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001043096.1 |
CG9218-RG, transcript variant G (sm), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_166370.3 |
CG9218-RD, transcript variant D (sm), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_166369.3 |
CG9218-RB, transcript variant B (sm), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001032268.1 |
CG9218-RF, transcript variant F (sm), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_136585.1 |
CG13745-RA (CG13745), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_145761.1 |
CG16778-RC, transcript variant C (Tkr), mRNA |
|
| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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