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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 4045R-2 
 Symbol CG4045  Full Name CG4045 
 CG No CG4045  Old CG No CG4045 
 Synonyms EG:22E5.4, CG4045 
 Accession No (Link to NCBI) NM_130622.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees male lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgACCAGGAGCCGGATGTGCTAA 
 Primer Seq. 3' 
aatctagaggtaccTTCGGAGAGTAGAGCCTGGTT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ACCAGGAGCC GGATGTGCTA AACCCCACGT CGGCGGTGAC GGGGTTGCCA CAAAAGCGCT 
0061 ACTACCGCCA GCGTGCGCAC TCGAATCCCA TTGCGGACCA CAGCTTCGAC TATCCTGCTC 
0121 GCCCGGAAGA TGTGGACTGG CGCTCCATGT ACCCAGGCAT TCAGCAGGGC CAGCAGGTAA 
0181 GTTTTGCGGA CATAGGCTGC GGCTATGGCG GCTTTCTCGT CACCCTGGGG GAGATGTTCC 
0241 CCGAGAAGCT GTCCATCGGC ATGGAAATCC GCGTCAAGGT GTCGGACTAC GTGGTGGACC 
0301 GGATTGCGGC ACTGCGTCGA CGATGCGCGG ATACGGGTGC ATACCAGAAC ATTGCCTGCT 
0361 TACGCACCAA CGCCATGAAG TATCTGCCCA ACTACTTCGT AAAGGGCCAG CTGGAGAAGA 
0421 TGTTCTTCCT CTACCCGGAC CCACATTTCA AGCGCGCCAA GCACAAGTGG CGTATTATCA 
0481 ACCAGGCTCT ACTCTCCGAA  
 in silico PCR Fragment 
0001 ACCAGGAGCC GGATGTGCTA AACCCCACGT CGGCGGTGAC GGGGTTGCCA CAAAAGCGCT 
0061 ACTACCGCCA GCGTGCGCAC TCGAATCCCA TTGCGGACCA CAGCTTCGAC TATCCTGCTC 
0121 GCCCGGAAGA TGTGGACTGG CGCTCCATGT ACCCAGGCAT TCAGCAGGGC CAGCAGGTAA 
0181 GTTTTGCGGA CATAGGCTGC GGCTATGGCG GCTTTCTCGT CACCCTGGGG GAGATGTTCC 
0241 CCGAGAAGCT GTCCATCGGC ATGGAAATCC GCGTCAAGGT GTCGGACTAC GTGGTGGACC 
0301 GGATTGCGGC ACTGCGTCGA CGATGCGCGG ATACGGGTGC ATACCAGAAC ATTGCCTGCT 
0361 TACGCACCAA CGCCATGAAG TATCTGCCCA ACTACTTCGT AAAGGGCCAG CTGGAGAAGA 
0421 TGTTCTTCCT CTACCCGGAC CCACATTTCA AGCGCGCCAA GCACAAGTGG CGTATTATCA 
0481 ACCAGGCTCT ACTCTCCGAA  
 Assemble Data 

4045R-2.IR_full       1   ACCAGGAGCCGGATGTGCTAAACCCCACGTCGGCGGTGACGGGGTTGCCACAAAAGCGCT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4045R-2.in silico     1   ACCAGGAGCCGGATGTGCTAAACCCCACGTCGGCGGTGACGGGGTTGCCACAAAAGCGCT 60


4045R-2.IR_full       61  ACTACCGCCAGCGTGCGCACTCGAATCCCATTGCGGACCACAGCTTCGACTATCCTGCTC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4045R-2.in silico     61  ACTACCGCCAGCGTGCGCACTCGAATCCCATTGCGGACCACAGCTTCGACTATCCTGCTC 120


4045R-2.IR_full       121 GCCCGGAAGATGTGGACTGGCGCTCCATGTACCCAGGCATTCAGCAGGGCCAGCAGGTAA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4045R-2.in silico     121 GCCCGGAAGATGTGGACTGGCGCTCCATGTACCCAGGCATTCAGCAGGGCCAGCAGGTAA 180


4045R-2.IR_full       181 GTTTTGCGGACATAGGCTGCGGCTATGGCGGCTTTCTCGTCACCCTGGGGGAGATGTTCC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4045R-2.in silico     181 GTTTTGCGGACATAGGCTGCGGCTATGGCGGCTTTCTCGTCACCCTGGGGGAGATGTTCC 240


4045R-2.IR_full       241 CCGAGAAGCTGTCCATCGGCATGGAAATCCGCGTCAAGGTGTCGGACTACGTGGTGGACC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4045R-2.in silico     241 CCGAGAAGCTGTCCATCGGCATGGAAATCCGCGTCAAGGTGTCGGACTACGTGGTGGACC 300


4045R-2.IR_full       301 GGATTGCGGCACTGCGTCGACGATGCGCGGATACGGGTGCATACCAGAACATTGCCTGCT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4045R-2.in silico     301 GGATTGCGGCACTGCGTCGACGATGCGCGGATACGGGTGCATACCAGAACATTGCCTGCT 360


4045R-2.IR_full       361 TACGCACCAACGCCATGAAGTATCTGCCCAACTACTTCGTAAAGGGCCAGCTGGAGAAGA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4045R-2.in silico     361 TACGCACCAACGCCATGAAGTATCTGCCCAACTACTTCGTAAAGGGCCAGCTGGAGAAGA 420


4045R-2.IR_full       421 TGTTCTTCCTCTACCCGGACCCACATTTCAAGCGCGCCAAGCACAAGTGGCGTATTATCA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4045R-2.in silico     421 TGTTCTTCCTCTACCCGGACCCACATTTCAAGCGCGCCAAGCACAAGTGGCGTATTATCA 480


4045R-2.IR_full       481 ACCAGGCTCTACTCTCCGAA 500
                          ||||||||||||||||||||
4045R-2.in silico     481 ACCAGGCTCTACTCTCCGAA 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_130622.2  CG4045-RA (CG4045), mRNA 
0   0  3  2  3  NM_142768.3  CG7073-RB, transcript variant B (sar1), mRNA 
0   0  3  2  3  NM_170001.2  CG7073-RC, transcript variant C (sar1), mRNA 
0   0  3  2  3  NM_170002.2  CG7073-RD, transcript variant D (sar1), mRNA 
0   0  3  2  3  NM_206542.1  CG7073-RE, transcript variant E (sar1), mRNA 
0   0  3  2  3  NM_170000.2  CG7073-RA, transcript variant A (sar1), mRNA 
0   0  2  5  16  NM_001032051.1  CG33715-RD, transcript variant D (Msp-300), mRNA 
0   0  2  5  13  NM_001032053.1  CG33715-RB, transcript variant B (Msp-300), mRNA 
0   0  2  3  3  NM_136133.2  CG10188-RB, transcript variant B (CG10188), mRNA 
0   0  2  3  3  NM_165305.2  CG10188-RA, transcript variant A (CG10188), mRNA 
0   0  2  2  5  NM_079571.1  CG8374-RA (dmt), mRNA 
0   0  2  2  2  NM_132975.2  CG12998-RA (CG12998), mRNA 
0   0  1  6  4  NM_137667.3  CG13442-RA (CG13442), mRNA 
0   0  1  4  2  NM_175994.1  CG32988-RA (CG32988), mRNA 
0   0  1  3  5  NM_137574.1  CG10081-RA (CG10081), mRNA 
0   0  1  3  2  NM_142100.2  CG8524-RA, transcript variant A (NK7.1), mRNA 
0   0  1  3  2  NM_169580.1  CG8524-RB, transcript variant B (NK7.1), mRNA 
0   0  1  2  4  NM_143149.1  CG5071-RB, transcript variant B (CG5071), mRNA 
0   0  1  2  4  NM_170232.1  CG5071-RA, transcript variant A (CG5071), mRNA 
0   0  1  2  2  NM_167228.1  CG32681-RA (CG32681), mRNA 
0   0  0  6  3  NM_136391.2  CG9436-RA (CG9436), mRNA 
0   0  0  6  3  NM_079118.2  CG3401-RA (betaTub60D), mRNA 
0   0  0  5  3  NM_136249.1  CG9248-RB, transcript variant B (CG9248), mRNA 
0   0  0  5  3  NM_165356.1  CG9248-RA, transcript variant A (CG9248), mRNA 
0   0  0  4  10  NM_165900.1  CG8830-RB, transcript variant B (CG8830), mRNA 
0   0  0  4  10  NM_136940.1  CG8830-RA, transcript variant A (CG8830), mRNA 
0   0  0  4  6  NM_079393.2  CG4083-RA (Mo25), mRNA 
0   0  0  4  5  NM_136862.1  CG8271-RA (CG8271), mRNA 
0   0  0  4  5  NM_057798.2  CG1650-RA (unpg), mRNA 
0   0  0  4  5  NM_142902.2  CG16723-RA (CG16723), mRNA 

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