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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4003R-1 |
| Symbol |
pont |
Full Name |
pontin |
| CG No |
CG4003 |
Old CG No |
CG4003 |
| Synonyms |
Tip49/Pontin, dPontin, CG4003, dPon, Tip49, pon, dpon, 0229/05, l(3)S022905, anon-WO0118547.698, pont, Pontin, Pont |
| Accession No (Link to NCBI) |
NM_144351.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
Wang X, Huang X, Wu C, Xue L.
Pontin/Tip49 negatively regulates JNK-mediated cell death in Drosophila.
Cell Death Discov (2018) 4 8
[
PubMed ID = 30062057
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCCAGGAGCTGGGTAACAAAG |
| Primer Seq. 3' |
aatctagaggtaccTGGACGAGCATTGGCCACATC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCCAGGAGCT GGGTAACAAA GTGCCCTTCT GTCCCATGGT CGGATCGGAG GTATTCAGCA
0061 ATGAGATCAA GAAGACCGAG GTGCTAATGG AGAATTTCCG GCGCTCCATT GGTCTGCGCA
0121 TTCGTGAGAC CAAAGAGGTG TACGAGGGCG AGGTTACGGA ACTCACGCCC GTGGAAACCG
0181 AGAATCCTAT GGGTGGCTAT GGCAAGACTA TCAGCAATGT CGTCATCGGC TTGAAAACAG
0241 CCAAGGGAAC CAAGCAGCTC AAGCTGGATC CCAGCATCTT TGATGCCTTG CAAAAGGAAA
0301 AGGTGGAGGT GGGCGATGTC ATCTACATAG AGGCCAACAG TGGTGCCGTA AAGCGTCAGG
0361 GTCGCAGTGA CACCTTTGCC ACTGAGTTTG ATCTCGAAAC CGAGGAGTAT GTGCCGCTGC
0421 CCAAGGGCGA TGTCCACAAG AAGAAGGAGG TTATCCAGGA TGTTACTCTG CACGATCTGG
0481 ATGTGGCCAA TGCTCGTCCA |
| in silico PCR Fragment |
0001 CCCAGGAGCT GGGTAACAAA GTGCCCTTCT GTCCCATGGT CGGATCGGAG GTATTCAGCA
0061 ATGAGATCAA GAAGACCGAG GTGCTAATGG AGAATTTCCG GCGCTCCATT GGTCTGCGCA
0121 TTCGTGAGAC CAAAGAGGTG TACGAGGGCG AGGTTACGGA ACTCACGCCC GTGGAAACCG
0181 AGAATCCTAT GGGTGGCTAT GGCAAGACTA TCAGCAATGT CGTCATCGGC TTGAAAACAG
0241 CCAAGGGAAC CAAGCAGCTC AAGCTGGATC CCAGCATCTT TGATGCCTTG CAAAAGGAAA
0301 AGGTGGAGGT GGGCGATGTC ATCTACATAG AGGCCAACAG TGGTGCCGTA AAGCGTCAGG
0361 GTCGCAGTGA CACCTTTGCC ACTGAGTTTG ATCTCGAAAC CGAGGAGTAT GTGCCGCTGC
0421 CCAAGGGCGA TGTCCACAAG AAGAAGGAGG TTATCCAGGA TGTTACTCTG CACGATCTGG
0481 ATGTGGCCAA TGCTCGTCCA |
| Assemble Data |
4003R-1.IR_full 1 CCCAGGAGCTGGGTAACAAAGTGCCCTTCTGTCCCATGGTCGGATCGGAGGTATTCAGCA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4003R-1.in silico 1 CCCAGGAGCTGGGTAACAAAGTGCCCTTCTGTCCCATGGTCGGATCGGAGGTATTCAGCA 60
4003R-1.IR_full 61 ATGAGATCAAGAAGACCGAGGTGCTAATGGAGAATTTCCGGCGCTCCATTGGTCTGCGCA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4003R-1.in silico 61 ATGAGATCAAGAAGACCGAGGTGCTAATGGAGAATTTCCGGCGCTCCATTGGTCTGCGCA 120
4003R-1.IR_full 121 TTCGTGAGACCAAAGAGGTGTACGAGGGCGAGGTTACGGAACTCACGCCCGTGGAAACCG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4003R-1.in silico 121 TTCGTGAGACCAAAGAGGTGTACGAGGGCGAGGTTACGGAACTCACGCCCGTGGAAACCG 180
4003R-1.IR_full 181 AGAATCCTATGGGTGGCTATGGCAAGACTATCAGCAATGTCGTCATCGGCTTGAAAACAG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4003R-1.in silico 181 AGAATCCTATGGGTGGCTATGGCAAGACTATCAGCAATGTCGTCATCGGCTTGAAAACAG 240
4003R-1.IR_full 241 CCAAGGGAACCAAGCAGCTCAAGCTGGATCCCAGCATCTTTGATGCCTTGCAAAAGGAAA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4003R-1.in silico 241 CCAAGGGAACCAAGCAGCTCAAGCTGGATCCCAGCATCTTTGATGCCTTGCAAAAGGAAA 300
4003R-1.IR_full 301 AGGTGGAGGTGGGCGATGTCATCTACATAGAGGCCAACAGTGGTGCCGTAAAGCGTCAGG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4003R-1.in silico 301 AGGTGGAGGTGGGCGATGTCATCTACATAGAGGCCAACAGTGGTGCCGTAAAGCGTCAGG 360
4003R-1.IR_full 361 GTCGCAGTGACACCTTTGCCACTGAGTTTGATCTCGAAACCGAGGAGTATGTGCCGCTGC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4003R-1.in silico 361 GTCGCAGTGACACCTTTGCCACTGAGTTTGATCTCGAAACCGAGGAGTATGTGCCGCTGC 420
4003R-1.IR_full 421 CCAAGGGCGATGTCCACAAGAAGAAGGAGGTTATCCAGGATGTTACTCTGCACGATCTGG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4003R-1.in silico 421 CCAAGGGCGATGTCCACAAGAAGAAGGAGGTTATCCAGGATGTTACTCTGCACGATCTGG 480
4003R-1.IR_full 481 ATGTGGCCAATGCTCGTCCA 500
||||||||||||||||||||
4003R-1.in silico 481 ATGTGGCCAATGCTCGTCCA 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
3 |
NM_144351.2 |
CG4003-RA (pont), mRNA |
| 0 |
0 |
3 |
5 |
3 |
NM_134748.2 |
CG5423-RA (robo3), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_078559.2 |
CG18085-RA (sev), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_140792.1 |
CG6896-RA (MYPT-75D), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_206753.1 |
CG9170-RB, transcript variant B (CG9170), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_132867.1 |
CG9170-RA, transcript variant A (CG9170), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_140327.1 |
CG10522-RA (sti), mRNA |
| 0 |
0 |
1 |
5 |
9 |
NM_078606.3 |
CG6146-RA, transcript variant A (Top1), mRNA |
| 0 |
0 |
1 |
5 |
9 |
NM_001014742.1 |
CG6146-RC, transcript variant C (Top1), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_078759.2 |
CG11020-RA, transcript variant A (nompC), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_205912.1 |
CG11020-RB, transcript variant B (nompC), mRNA |
| 0 |
0 |
1 |
4 |
12 |
NM_132457.1 |
CG11122-RA (CG11122), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_143223.1 |
CG14238-RA (CG14238), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_166551.2 |
CG30267-RA (CG30267), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_137238.2 |
CG8403-RA (SP2353), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_140192.1 |
CG7607-RA (CG7607), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_143807.2 |
CG3558-RA, transcript variant A (CG3558), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_164519.1 |
CG3558-RB, transcript variant B (CG3558), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_142385.1 |
CG14322-RA (CG14322), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_135722.2 |
CG6388-RA (CG6388), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_135723.2 |
CG5435-RA (CG5435), mRNA |
| 0 |
0 |
0 |
9 |
5 |
NM_134718.4 |
CG31795-RA, transcript variant A (ia2), mRNA |
| 0 |
0 |
0 |
9 |
5 |
NM_164401.3 |
CG31795-RB, transcript variant B (ia2), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_140725.1 |
CG18265-RA (CG18265), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_134917.2 |
CG15406-RA (CG15406), mRNA |
| 0 |
0 |
0 |
5 |
8 |
NM_169450.1 |
CG14741-RA (CG14741), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_132685.1 |
CG9940-RA, transcript variant A (CG9940), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_167378.1 |
CG9940-RB, transcript variant B (CG9940), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_079903.2 |
CG15319-RB (nej), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_168056.1 |
CG14994-RC, transcript variant C (Gad1), mRNA |
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