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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3998R-2 |
| Symbol |
zf30C |
Full Name |
Zinc finger protein 30C |
| CG No |
CG3998 |
Old CG No |
CG3998 |
| Synonyms |
CG3998, l(2)k02506, clone 1.52, anon-EST:Liang-1.52, zf30C, Zf30c |
| Accession No (Link to NCBI) |
NM_057953.3 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
male lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Molnar C, Casado M, López-Varea A, Cruz C, de Celis JF.
Genetic annotation of gain-of-function screens using RNA interference and in situ hybridization of candidate genes in the Drosophila wing.
Genetics (2012) 192(2) 741-52
[
PubMed ID = 22798488
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCGAGTCGATGGCTCCAAGCAG |
| Primer Seq. 3' |
aatctagaggtaccCGGATGAACCGGGCGAATCGT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CGAGTCGATG GCTCCAAGCA GCAGTgcNGC gGCTgNAGcC ACCAAGCTCC TGGTGGAGAT
0061 CACCACCGAC GGAATACCCA AGCTGCACTG CCCGGTGTGC AACAAGGCGC TGGTCTCCCT
0121 AGCCGGATAT GTGAAGCACG TAAAGAAGCA CCAGCCGCCG ggcGNCNTCG NAGTGCCGtC
0181 ACTGCGATGC TCGGTTTTGC CACGAGGAGG AGCTCACCCA GCATGCAAAG GATGACGCAC
0241 GGAgTCACTG GCGCGGTAGC TGNGACAGGA GCGAAAGCCT TTCGTTTGCG AAAAGTGCGG
0301 CGCGGAGTAC AAGTACCAGG AGGCATANCC GTCGCCACTG CAGGACCAAG TGTGGCGAGG
0361 AAAAGCTACC CAGGGAGGAA TCCCGACCAA TGGAGTGCAA GTGCNTGCTA CACCCGCTTC
0421 TCTAGCGCCT CTAACCTATC CAAGCATCGA CGCAGtCGTC CCGACACCTG TGGACAGCCG
0481 GAATACGATT CGCCCGGTTC ATCCG |
| in silico PCR Fragment |
0001 CGAGTCGATG GCTCCAAGCA GCAGTGCGGC GGCTGCAGCC ACCAAGCTCC TGGTGGAGAT
0061 CACCACCGAC GGAATACCCA AGCTGCACTG CCCGGTGTGC AACAAGGCGC TGGTCTCCCT
0121 AGCCGGATAT GTGAAGCACG TAAAGAAGCA CCAGCCGCCG GGCGGCTTCG -AGTGCCGTC
0181 ACTGCGATGC TCGGTTTTGC CACGAGGAGG AGCTCACCCA GCATGCAAAG GATGA-GCAC
0241 GGAGTCACTG GCGCGGTAGC TG-GACAGGA GCGAAAGCCT TTCGTTTGTG AAAAGTGCGG
0301 CGCGGAGTAC AAGTACCAGG AGGCATA-CC GTCGCCACTG CAGGACCAAG TGTGGCGAGG
0361 AAAAGCTACC CAGGGAGGAA TCCCGACCAA TGGAGTGCAA GTGC-TGCTA CACCCGCTTC
0421 TCTAGCGCCT CTAACCTATC CAAGCATCGA CGCAGTCGTC CCGACACCTG TGGACAGCCG
0481 GAATACGATT CGCCCGGTTC ATCCG |
| Assemble Data |
3998R-2.IR_full 1 CGAGTCGATGGCTCCAAGCAGCAGTGCNGCGGCTGNAGCCACCAAGCTCCTGGTGGAGAT 60
||||||||||||||||||||||||||| ||||||| ||||||||||||||||||||||||
3998R-2.in silico 1 CGAGTCGATGGCTCCAAGCAGCAGTGCGGCGGCTGCAGCCACCAAGCTCCTGGTGGAGAT 60
3998R-2.IR_full 61 CACCACCGACGGAATACCCAAGCTGCACTGCCCGGTGTGCAACAAGGCGCTGGTCTCCCT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3998R-2.in silico 61 CACCACCGACGGAATACCCAAGCTGCACTGCCCGGTGTGCAACAAGGCGCTGGTCTCCCT 120
3998R-2.IR_full 121 AGCCGGATATGTGAAGCACGTAAAGAAGCACCAGCCGCCGGGCGNCNTCGNAGTGCCGTC 180
|||||||||||||||||||||||||||||||||||||||||||| | ||| |||||||||
3998R-2.in silico 121 AGCCGGATATGTGAAGCACGTAAAGAAGCACCAGCCGCCGGGCGGCTTCG-AGTGCCGTC 180
3998R-2.IR_full 181 ACTGCGATGCTCGGTTTTGCCACGAGGAGGAGCTCACCCAGCATGCAAAGGATGACGCAC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
3998R-2.in silico 181 ACTGCGATGCTCGGTTTTGCCACGAGGAGGAGCTCACCCAGCATGCAAAGGATGA-GCAC 240
3998R-2.IR_full 241 GGAGTCACTGGCGCGGTAGCTGNGACAGGAGCGAAAGCCTTTCGTTTGCGAAAAGTGCGG 300
|||||||||||||||||||||| ||||||||||||||||||||||||| |||||||||||
3998R-2.in silico 241 GGAGTCACTGGCGCGGTAGCTG-GACAGGAGCGAAAGCCTTTCGTTTGTGAAAAGTGCGG 300
3998R-2.IR_full 301 CGCGGAGTACAAGTACCAGGAGGCATANCCGTCGCCACTGCAGGACCAAGTGTGGCGAGG 360
||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
3998R-2.in silico 301 CGCGGAGTACAAGTACCAGGAGGCATA-CCGTCGCCACTGCAGGACCAAGTGTGGCGAGG 360
3998R-2.IR_full 361 AAAAGCTACCCAGGGAGGAATCCCGACCAATGGAGTGCAAGTGCNTGCTACACCCGCTTC 420
|||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||
3998R-2.in silico 361 AAAAGCTACCCAGGGAGGAATCCCGACCAATGGAGTGCAAGTGC-TGCTACACCCGCTTC 420
3998R-2.IR_full 421 TCTAGCGCCTCTAACCTATCCAAGCATCGACGCAGTCGTCCCGACACCTGTGGACAGCCG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3998R-2.in silico 421 TCTAGCGCCTCTAACCTATCCAAGCATCGACGCAGTCGTCCCGACACCTGTGGACAGCCG 480
3998R-2.IR_full 481 GAATACGATTCGCCCGGTTCATCCG 505
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3998R-2.in silico 481 GAATACGATTCGCCCGGTTCATCCG 505
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
4 |
NM_057953.3 |
CG3998-RA (zf30C), mRNA |
| 0.2 |
1 |
2 |
2 |
5 |
NM_143508.1 |
CG7928-RA (CG7928), mRNA |
| 0 |
0 |
4 |
5 |
2 |
NM_132830.1 |
CG15601-RA (CG15601), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_141658.3 |
CG8301-RA (CG8301), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_080535.2 |
CG9774-RA (rok), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_142709.2 |
CG10823-RA, transcript variant A (SIFR), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_169955.2 |
CG10823-RB, transcript variant B (SIFR), mRNA |
| 0 |
0 |
1 |
6 |
10 |
NM_132259.2 |
CG11219-RA (PIP82), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_001032250.1 |
CG30084-RE, transcript variant E (CG30084), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_001043086.1 |
CG30084-RG, transcript variant G (CG30084), mRNA |
| 0 |
0 |
1 |
4 |
8 |
NM_165262.2 |
CG31753-RA (ham), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_140803.1 |
CG14073-RB, transcript variant B (CG14073), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_164735.1 |
CG11266-RG, transcript variant G (CG11266), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_135251.2 |
CG11266-RB, transcript variant B (CG11266), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_164730.1 |
CG11266-RA, transcript variant A (CG11266), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_164733.1 |
CG11266-RD, transcript variant D (CG11266), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_164731.1 |
CG11266-RE, transcript variant E (CG11266), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_164734.1 |
CG11266-RF, transcript variant F (CG11266), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_164732.1 |
CG11266-RC, transcript variant C (CG11266), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_134486.1 |
CG14221-RA (CG14221), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_176132.2 |
CG12052-RR, transcript variant R (lola), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_080027.4 |
CG12052-RG, transcript variant G (lola), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_080331.2 |
CG3578-RA (bi), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_132252.3 |
CG1474-RA (Es2), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_138132.1 |
CG9083-RA (CG9083), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_164830.3 |
CG18405-RA, transcript variant A (Sema-1a), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_135399.2 |
CG18405-RB, transcript variant B (Sema-1a), mRNA |
| 0 |
0 |
0 |
8 |
3 |
NM_080190.2 |
CG4152-RA (l(2)35Df), mRNA |
| 0 |
0 |
0 |
6 |
11 |
NM_001032051.1 |
CG33715-RD, transcript variant D (Msp-300), mRNA |
| 0 |
0 |
0 |
6 |
6 |
NM_132578.1 |
CG11146-RA (CG11146), mRNA |
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