NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 3966R-2

Symbol ninaA Full Name neither inactivation nor afterpotential A

CG No CG3966 Old CG No CG3966

Synonyms ninA, CG3966, ninaA, NinaA

Accession No (Link to NCBI) NM_057308.3

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGATAATCCTGTGCAGCGCCTT

Primer Seq. 3'

aatctagaggtaccCATCCTCAATGGCATAGATGG

Predicted Fragment Size

IR fragment full Seq

0001 GATAATCCTG TGCAGCgNCT TTCTGGCCGT GGccAGTCGG TCTGAGCTTC ACGGTCACGT 
0061 CTCGGATCTA CATGGATGTG AAGCACAACA AGAAGCCGGT gGGCAGGATC ACGTTTGGAC 
0121 TGTTCGGGAA GCTGGCTCCC AAGACGGTGG CCAACTTCCG GCACATTTGC TTGCGCGGCA 
0181 TCAACGGGAC CAGCTACGTG GGCTCGCNAT TCCATCGCGT GGTGGACCGC TTTCTCGTCC 
0241 AAGGCGGCGA CATTGTGAAC GGCGACGGAA CTGGCTCCAT TAGCATCTAT GGGGACTACT 
0301 TTCCGGACGA GGATAAGGCT CTGGCGGTGG AGCACAACAG ACCCGGTTAC TTGGGCATGG 
0361 CCAATCGGGG CCCGGACACC AATGGTTGCC AGTTTTATGT GACCACCGTG GGCGCCAAGT 
0421 GGCTGGACGG AAAGCACACC GTTTTCGGCA AGGTGCTGGA GGGAATGGAC ACCATCTATG 
0481 CCATTGAGGA TG

in silico PCR Fragment

0001 GATAATCCTG TGCAGCGCCT TTCTGGCCGT GGCCAGT-GG TCTGAGCTTC ACGGTCACGT 
0061 CTCGGATCTA CATGGATGTG AAGCACAACA AGAAGCCGGT GGGCAGGATC ACGTTTGGAC 
0121 TGTTCGGGAA GCTGGCTCCC AAGACGGTGG CCAACTTCCG GCACATTTGC TTGCGCGGCA 
0181 TCAACGGGAC CAGCTACGTG GGCTCGCGAT TCCATCGCGT GGTGGACCGC TTTCTCGTCC 
0241 AAGGCGGCGA CATTGTGAAC GGCGACGGAA CTGGCTCCAT TAGCATCTAT GGGGACTACT 
0301 TTCCGGACGA GGATAAGGCT CTGGCGGTGG AGCACAACAG ACCCGGTTAC TTGGGCATGG 
0361 CCAATCGGGG CCCGGACACC AATGGCTGCC AGTTTTATGT GACCACCGTG GGCGCCAAGT 
0421 GGCTGGACGG AAAGCACACC GTTTTCGGCA AGGTGCTGGA GGGAATGGAC ACCATCTATG 
0481 CCATTGAGGA TG

Assemble Data


3966R-2.IR_full       1   GATAATCCTGTGCAGCGNCTTTCTGGCCGTGGCCAGTCGGTCTGAGCTTCACGGTCACGT 60
                          ||||||||||||||||| ||||||||||||||||||| ||||||||||||||||||||||
3966R-2.in silico     1   GATAATCCTGTGCAGCGCCTTTCTGGCCGTGGCCAGT-GGTCTGAGCTTCACGGTCACGT 60


3966R-2.IR_full       61  CTCGGATCTACATGGATGTGAAGCACAACAAGAAGCCGGTGGGCAGGATCACGTTTGGAC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3966R-2.in silico     61  CTCGGATCTACATGGATGTGAAGCACAACAAGAAGCCGGTGGGCAGGATCACGTTTGGAC 120


3966R-2.IR_full       121 TGTTCGGGAAGCTGGCTCCCAAGACGGTGGCCAACTTCCGGCACATTTGCTTGCGCGGCA 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3966R-2.in silico     121 TGTTCGGGAAGCTGGCTCCCAAGACGGTGGCCAACTTCCGGCACATTTGCTTGCGCGGCA 180


3966R-2.IR_full       181 TCAACGGGACCAGCTACGTGGGCTCGCNATTCCATCGCGTGGTGGACCGCTTTCTCGTCC 240
                          ||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
3966R-2.in silico     181 TCAACGGGACCAGCTACGTGGGCTCGCGATTCCATCGCGTGGTGGACCGCTTTCTCGTCC 240


3966R-2.IR_full       241 AAGGCGGCGACATTGTGAACGGCGACGGAACTGGCTCCATTAGCATCTATGGGGACTACT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3966R-2.in silico     241 AAGGCGGCGACATTGTGAACGGCGACGGAACTGGCTCCATTAGCATCTATGGGGACTACT 300


3966R-2.IR_full       301 TTCCGGACGAGGATAAGGCTCTGGCGGTGGAGCACAACAGACCCGGTTACTTGGGCATGG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3966R-2.in silico     301 TTCCGGACGAGGATAAGGCTCTGGCGGTGGAGCACAACAGACCCGGTTACTTGGGCATGG 360


3966R-2.IR_full       361 CCAATCGGGGCCCGGACACCAATGGTTGCCAGTTTTATGTGACCACCGTGGGCGCCAAGT 420
                          ||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
3966R-2.in silico     361 CCAATCGGGGCCCGGACACCAATGGCTGCCAGTTTTATGTGACCACCGTGGGCGCCAAGT 420


3966R-2.IR_full       421 GGCTGGACGGAAAGCACACCGTTTTCGGCAAGGTGCTGGAGGGAATGGACACCATCTATG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3966R-2.in silico     421 GGCTGGACGGAAAGCACACCGTTTTCGGCAAGGTGCTGGAGGGAATGGACACCATCTATG 480


3966R-2.IR_full       481 CCATTGAGGATG 492
                          ||||||||||||
3966R-2.in silico     481 CCATTGAGGATG 492

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	473	0	0	2	NM_057308.3	CG3966-RA (ninaA), mRNA
0	0	3	3	8	NM_206586.1	CG31038-RC, transcript variant C (CG31038), mRNA
0	0	3	3	8	NM_206585.1	CG31038-RE, transcript variant E (CG31038), mRNA
0	0	3	3	8	NM_206584.1	CG31038-RF, transcript variant F (CG31038), mRNA
0	0	3	3	8	NM_170447.1	CG31038-RA, transcript variant A (CG31038), mRNA
0	0	1	4	2	NM_058073.3	CG17654-RA, transcript variant A (Eno), mRNA
0	0	1	4	2	NM_164433.1	CG17654-RD, transcript variant D (Eno), mRNA
0	0	1	4	2	NM_164432.1	CG17654-RC, transcript variant C (Eno), mRNA
0	0	1	4	2	NM_164434.1	CG17654-RE, transcript variant E (Eno), mRNA
0	0	1	4	2	NM_164431.1	CG17654-RB, transcript variant B (Eno), mRNA
0	0	1	3	2	NM_165063.1	CG8978-RB, transcript variant B (Sop2), mRNA
0	0	1	3	2	NM_057248.3	CG8978-RA, transcript variant A (Sop2), mRNA
0	0	1	3	2	NM_080048.2	CG10109-RA (L), mRNA
0	0	1	2	10	NM_167075.1	CG3314-RC, transcript variant C (RpL7A), mRNA
0	0	1	2	10	NM_167073.1	CG3314-RA, transcript variant A (RpL7A), mRNA
0	0	1	2	10	NM_078508.4	CG3314-RD, transcript variant D (RpL7A), mRNA
0	0	1	2	8	NM_141965.1	CG14395-RA (CG14395), mRNA
0	0	1	2	5	NM_167094.1	CG4532-RE, transcript variant E (pod1), mRNA
0	0	1	2	5	NM_132124.4	CG4532-RD, transcript variant D (pod1), mRNA
0	0	1	2	5	NM_167093.1	CG4532-RC, transcript variant C (pod1), mRNA
0	0	1	2	5	NM_167092.1	CG4532-RB, transcript variant B (pod1), mRNA
0	0	1	2	5	NM_167091.1	CG4532-RA, transcript variant A (pod1), mRNA
0	0	1	2	3	NM_167617.1	CG32548-RB, transcript variant B (CG32548), mRNA
0	0	1	2	3	NM_133065.2	CG32548-RC, transcript variant C (CG32548), mRNA
0	0	1	2	2	NM_139839.3	CG32381-RA (unc-13-4A), mRNA
0	0	1	2	2	NM_143050.1	CG11069-RA (CG11069), mRNA
0	0	0	6	4	NM_130655.1	CG8636-RA (CG8636), mRNA
0	0	0	5	4	NM_141388.2	CG1249-RA (CG1249), mRNA
0	0	0	5	2	NM_132522.2	CG32662-RA (CG32662), mRNA
0	0	0	5	2	NM_167438.1	CG9151-RB, transcript variant B (acj6), mRNA

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