|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3940R-1 |
| Symbol |
CG3940 |
Full Name |
CG3940 |
| CG No |
CG3940 |
Old CG No |
CG3940 |
| Synonyms |
CG3940 |
| Accession No (Link to NCBI) |
NM_141722.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Molnar C, Casado M, López-Varea A, Cruz C, de Celis JF.
Genetic annotation of gain-of-function screens using RNA interference and in situ hybridization of candidate genes in the Drosophila wing.
Genetics (2012) 192(2) 741-52
[
PubMed ID = 22798488
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCTTAATAGTCTGCTGCTCTG |
| Primer Seq. 3' |
aatctagaggtaccGATGCTACCTATGGCCTCGTT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCTTAATAGT CTGCTGCTCT GTTTCGTTTA GCTGGGCCAA CGAATGGGGC TATCCGGACT
0061 TGGACAACAA CCAGGATGAG CCATTTCCAA AATGGGGCGG TCTTTGCGAT AGCGGAAAGA
0121 AGCAAAGCCC CATAAATTTG CACGTCAAAG GCGCACTGAA GGGAGAGTTT GATGCCCTGA
0181 AGTTCGAGAA CTATGACGAG CATCAGAAGA ATCTGCGAAT GGTCAACAAT GGACATTCAA
0241 TTCAACTATC TGGCTTCGAT CACGAACTGA CCCTGAGTGG TGGTGCCCTG CTGCAAGACT
0301 TTGTGGTGGA GCAGATCCAC ATGCATTGGT GGTCGGAGCA CACCATCAAC GACATCCGGT
0361 ATCCGCTGGA AGTGCACATC GTGCACCGGA ATACCATATA CCCGAACATG ACAATGGCGG
0421 CCAACTTTAA AGATGGCATT GTGGTCATTG GAGTGCTGTA CCATGTGTCC AACACGCCCA
0481 ACGAGGCCAT AGGTAGCATC |
| in silico PCR Fragment |
0001 CCTTAATAGT CTGCTGCTCT GTTTCGTTTA GCTGGGCCAA CGAATGGGGC TATCCGGACT
0061 TGGACAACAA CCAGGATGAG CCATTTCCAA AATGGGGCGG TCTTTGCGAT AGCGGAAAGA
0121 AGCAAAGCCC CATAAATTTG CACGTCAAAG GCGCACTGAA GGGAGAGTTT GATGCCCTGA
0181 AGTTCGAGAA CTATGACGAG CATCAGAAGA ATCTGCGAAT GGTCAACAAT GGACATTCAA
0241 TTCAACTATC TGGCTTCGAT CACGAACTGA CCCTGAGTGG TGGTGCCCTG CTGCAAGACT
0301 TTGTGGTGGA GCAGATCCAC ATGCATTGGT GGTCGGAGCA CACCATCAAC GACATCCGGT
0361 ATCCGCTGGA AGTGCACATC GTGCACCGGA ATACCATATA CCCGAACATG ACAATGGCGG
0421 CCAACTTTAA AGATGGCATT GTGGTCATTG GAGTGCTGTA CCATGTGTCC AACACGCCCA
0481 ACGAGGCCAT AGGTAGCATC |
| Assemble Data |
3940R-1.IR_full 1 CCTTAATAGTCTGCTGCTCTGTTTCGTTTAGCTGGGCCAACGAATGGGGCTATCCGGACT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3940R-1.in silico 1 CCTTAATAGTCTGCTGCTCTGTTTCGTTTAGCTGGGCCAACGAATGGGGCTATCCGGACT 60
3940R-1.IR_full 61 TGGACAACAACCAGGATGAGCCATTTCCAAAATGGGGCGGTCTTTGCGATAGCGGAAAGA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3940R-1.in silico 61 TGGACAACAACCAGGATGAGCCATTTCCAAAATGGGGCGGTCTTTGCGATAGCGGAAAGA 120
3940R-1.IR_full 121 AGCAAAGCCCCATAAATTTGCACGTCAAAGGCGCACTGAAGGGAGAGTTTGATGCCCTGA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3940R-1.in silico 121 AGCAAAGCCCCATAAATTTGCACGTCAAAGGCGCACTGAAGGGAGAGTTTGATGCCCTGA 180
3940R-1.IR_full 181 AGTTCGAGAACTATGACGAGCATCAGAAGAATCTGCGAATGGTCAACAATGGACATTCAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3940R-1.in silico 181 AGTTCGAGAACTATGACGAGCATCAGAAGAATCTGCGAATGGTCAACAATGGACATTCAA 240
3940R-1.IR_full 241 TTCAACTATCTGGCTTCGATCACGAACTGACCCTGAGTGGTGGTGCCCTGCTGCAAGACT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3940R-1.in silico 241 TTCAACTATCTGGCTTCGATCACGAACTGACCCTGAGTGGTGGTGCCCTGCTGCAAGACT 300
3940R-1.IR_full 301 TTGTGGTGGAGCAGATCCACATGCATTGGTGGTCGGAGCACACCATCAACGACATCCGGT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3940R-1.in silico 301 TTGTGGTGGAGCAGATCCACATGCATTGGTGGTCGGAGCACACCATCAACGACATCCGGT 360
3940R-1.IR_full 361 ATCCGCTGGAAGTGCACATCGTGCACCGGAATACCATATACCCGAACATGACAATGGCGG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3940R-1.in silico 361 ATCCGCTGGAAGTGCACATCGTGCACCGGAATACCATATACCCGAACATGACAATGGCGG 420
3940R-1.IR_full 421 CCAACTTTAAAGATGGCATTGTGGTCATTGGAGTGCTGTACCATGTGTCCAACACGCCCA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3940R-1.in silico 421 CCAACTTTAAAGATGGCATTGTGGTCATTGGAGTGCTGTACCATGTGTCCAACACGCCCA 480
3940R-1.IR_full 481 ACGAGGCCATAGGTAGCATC 500
||||||||||||||||||||
3940R-1.in silico 481 ACGAGGCCATAGGTAGCATC 500
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_141722.1 |
CG3940-RA (CG3940), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_166631.1 |
CG5594-RA, transcript variant A (CG5594), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_166630.1 |
CG5594-RD, transcript variant D (CG5594), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_001042874.1 |
CG14034-RB (CG14034), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_079344.2 |
CG3836-RA (stwl), mRNA |
| 0 |
0 |
1 |
5 |
14 |
NM_143278.1 |
CG6074-RA (CG6074), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_141403.1 |
CG15185-RA (CG15185), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_141695.1 |
CG9467-RA (CG9467), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_142904.2 |
CG10184-RA (CG10184), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_058082.2 |
CG6193-RA (Apc2), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_142801.1 |
CG5346-RA (CG5346), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_140112.1 |
CG14165-RA (CG14165), mRNA |
| 0 |
0 |
0 |
8 |
42 |
NM_135002.1 |
CG15635-RA (CG15635), mRNA |
| 0 |
0 |
0 |
6 |
9 |
NM_132924.2 |
CG4678-RA, transcript variant A (CG4678), mRNA |
| 0 |
0 |
0 |
6 |
9 |
NM_001038761.1 |
CG4678-RC, transcript variant C (CG4678), mRNA |
| 0 |
0 |
0 |
6 |
9 |
NM_167536.2 |
CG4678-RB, transcript variant B (CG4678), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_080175.2 |
CG12242-RA (GstD5), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_137932.2 |
CG9868-RA (Prosbeta5R1), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_206496.1 |
CG14869-RB, transcript variant B (CG14869), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_169659.2 |
CG14869-RA, transcript variant A (CG14869), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_135027.2 |
CG3008-RA (CG3008), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_079595.2 |
CG6920-RA (mus309), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_141817.2 |
CG5276-RA (CG5276), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_001032231.1 |
CG18003-RA, transcript variant A (CG18003), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_001032230.1 |
CG18003-RB, transcript variant B (CG18003), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_001032232.1 |
CG11919-RA, transcript variant A (CG11919), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_140519.2 |
CG7739-RA (CG7739), mRNA |
| 0 |
0 |
0 |
3 |
11 |
NM_078804.2 |
CG4600-RA (yip2), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_080306.2 |
CG3443-RB (pcx), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_135077.2 |
CG14023-RA (CG14023), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| + |
|
| DC |
 |
| + |
|
| DM |
 |
| + |
|
| VM |
 |
| |
|
| VB |
 |
| |
|
|
|
|
|
|
