NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 3935R-3

Symbol al Full Name aristaless

CG No CG3935 Old CG No CG3935

Synonyms A1, CG3935, L13-4, AL, lambda13-4, al

Accession No (Link to NCBI) NM_164382.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGAGAAGGCATTCTCGCGGAC

Primer Seq. 3'

aatctagaggtaccGAGATCCCACCAGTAGTGCAG

Predicted Fragment Size

IR fragment full Seq

0001 GNAGAAGGCA TTCTCGCGGA CCCATTATCC CGACGTGTTT ACGAGAGAGG AGCTGGCGAT 
0061 GAAAATTGGA TTAACCGAGG CGCGCATTCA GGTTTGGTTT CAAAATCGGC NCGcCAAGTG 
0121 GCGCAAGCAG GAGAAGGTTG GCCCCCAGTC GCATCCGTAT AATCCCTTAC CTCGCCCGgc 
0181 GGCGCCGCCA CCATGCAGAC GGTAGTTGGG GCCGCCCTAC CGCCCAATCC CTTCACTCAC 
0241 CTGGGCTTCC AGCTGCGCAA GCCCTTCGAC GCCCAACATG CCGCGAACCT TGCCGCCTTC 
0301 CGCTACCCGC ACCTCTCTGC GGCCCCCATG ATTCCGTCGG GCTACTTTAA CCAATTCCAG 
0361 AGGGCTCGCT CCCCATATGT TNACCACATG GAATGGCTGG TATGTACTCT CCCTCGAGCT 
0421 CATTTCAATC CTTGCTGGCC AATATGACGG CCGTTCCGCG CGGGACTCCA NCTTGGAAAA 
0481 CCTCCTGNAC TACtgGTGGG ATcTC

in silico PCR Fragment

0001 GGAGAAGGCA TTCTCGCGGA CCCATTATCC CGACGTGTTT ACGAGAGAGG AGCTGGCGAT 
0061 GAAAATTGGA TTAACCGAGG CGCGCATTCA GGTTTGGTTT CAAAATCGGC GCGCCAAGTG 
0121 GCGCAAGCAG GAGAAGGTTG GCCCCCAGTC GCATCCGTAT AATCCC-TAC CT-GCCCGGC 
0181 GGCGCCGCCA CCATGCAGAC GGTAGTTGGG GCCGCCCTAC CGCCCAATCC CTTCACTCAC 
0241 CTGGGCTTCC AGCTGCGCAA GCCCTTCGAC GCCCAACATG CCGCGAACCT TGCCGCCTTC 
0301 CGCTACCCGC ACCTCTCTGC GGCCCCCATG ATTCCGTCGG GCTACTTTAA CCAATTCCAG 
0361 AGGGCTC-CT CCCCATATGT T-ACCACATG GAATGGCTGG TATGTACTCT CCCTCGAGCT 
0421 CATTTCAATC CTTGCTGGCC AATATGACGG CCGTTCCGCG CGGGACTCCA -CTTGGAAAA 
0481 CCTCCTGCAC TACTGGTGGG ATCTC

Assemble Data


3935R-3.IR_full       1   GNAGAAGGCATTCTCGCGGACCCATTATCCCGACGTGTTTACGAGAGAGGAGCTGGCGAT 60
                          | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3935R-3.in silico     1   GGAGAAGGCATTCTCGCGGACCCATTATCCCGACGTGTTTACGAGAGAGGAGCTGGCGAT 60


3935R-3.IR_full       61  GAAAATTGGATTAACCGAGGCGCGCATTCAGGTTTGGTTTCAAAATCGGCNCGCCAAGTG 120
                          |||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
3935R-3.in silico     61  GAAAATTGGATTAACCGAGGCGCGCATTCAGGTTTGGTTTCAAAATCGGCGCGCCAAGTG 120


3935R-3.IR_full       121 GCGCAAGCAGGAGAAGGTTGGCCCCCAGTCGCATCCGTATAATCCCTTACCTCGCCCGGC 180
                          |||||||||||||||||||||||||||||||||||||||||||||| ||||| |||||||
3935R-3.in silico     121 GCGCAAGCAGGAGAAGGTTGGCCCCCAGTCGCATCCGTATAATCCC-TACCT-GCCCGGC 180


3935R-3.IR_full       181 GGCGCCGCCACCATGCAGACGGTAGTTGGGGCCGCCCTACCGCCCAATCCCTTCACTCAC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3935R-3.in silico     181 GGCGCCGCCACCATGCAGACGGTAGTTGGGGCCGCCCTACCGCCCAATCCCTTCACTCAC 240


3935R-3.IR_full       241 CTGGGCTTCCAGCTGCGCAAGCCCTTCGACGCCCAACATGCCGCGAACCTTGCCGCCTTC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3935R-3.in silico     241 CTGGGCTTCCAGCTGCGCAAGCCCTTCGACGCCCAACATGCCGCGAACCTTGCCGCCTTC 300


3935R-3.IR_full       301 CGCTACCCGCACCTCTCTGCGGCCCCCATGATTCCGTCGGGCTACTTTAACCAATTCCAG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3935R-3.in silico     301 CGCTACCCGCACCTCTCTGCGGCCCCCATGATTCCGTCGGGCTACTTTAACCAATTCCAG 360


3935R-3.IR_full       361 AGGGCTCGCTCCCCATATGTTNACCACATGGAATGGCTGGTATGTACTCTCCCTCGAGCT 420
                          ||||||| ||||||||||||| ||||||||||||||||||||||||||||||||||||||
3935R-3.in silico     361 AGGGCTC-CTCCCCATATGTT-ACCACATGGAATGGCTGGTATGTACTCTCCCTCGAGCT 420


3935R-3.IR_full       421 CATTTCAATCCTTGCTGGCCAATATGACGGCCGTTCCGCGCGGGACTCCANCTTGGAAAA 480
                          |||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
3935R-3.in silico     421 CATTTCAATCCTTGCTGGCCAATATGACGGCCGTTCCGCGCGGGACTCCA-CTTGGAAAA 480


3935R-3.IR_full       481 CCTCCTGNACTACTGGTGGGATCTC 505
                          ||||||| |||||||||||||||||
3935R-3.in silico     481 CCTCCTGCACTACTGGTGGGATCTC 505

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	3	NM_164382.1	CG3935-RA (al), mRNA
1.24	6	8	6	14	NM_057982.3	CG2819-RA (Pph13), mRNA
0.2	1	4	11	9	NM_132272.1	CG11294-RA (CG11294), mRNA
0	0	6	6	4	NM_206591.1	CG1447-RC, transcript variant C (Ptx1), mRNA
0	0	6	6	4	NM_170531.3	CG1447-RA, transcript variant A (Ptx1), mRNA
0	0	6	6	4	NM_176593.1	CG1447-RB, transcript variant B (Ptx1), mRNA
0	0	5	7	3	NM_057601.2	CG2851-RA (Gsc), mRNA
0	0	5	5	6	NM_057678.3	CG31240-RA (repo), mRNA
0	0	4	13	20	NM_001038743.1	CG33980-RA (CG33980), mRNA
0	0	3	7	6	NM_057798.2	CG1650-RA (unpg), mRNA
0	0	3	2	3	NM_136624.2	CG8026-RB, transcript variant B (CG8026), mRNA
0	0	3	2	3	NM_165664.1	CG8026-RA, transcript variant A (CG8026), mRNA
0	0	2	11	2	NM_142681.1	CG7056-RA (CG7056), mRNA
0	0	2	5	13	NM_166413.2	CG10052-RA (Rx), mRNA
0	0	2	4	4	NM_132678.2	CG2691-RA (CG2691), mRNA
0	0	2	2	6	NM_205898.1	CG33281-RA (CG33281), mRNA
0	0	2	2	5	NM_139420.1	CG12361-RA (CG12361), mRNA
0	0	1	4	2	NM_143366.2	CG9986-RA (CG9986), mRNA
0	0	1	3	4	NM_001042822.1	CG34120-RC, transcript variant C (CG34120), mRNA
0	0	1	3	3	NM_057280.3	CG8695-RA (LvpL), mRNA
0	0	1	3	3	NM_144377.1	CG3706-RA (CG3706), mRNA
0	0	1	2	3	NM_079613.2	CG7642-RA (ry), mRNA
0	0	1	2	2	NM_167309.1	CG32663-RA (CG32663), mRNA
0	0	1	2	2	NM_206767.1	CG9132-RB, transcript variant B (CG9132), mRNA
0	0	1	2	2	NM_206768.1	CG9132-RA, transcript variant A (CG9132), mRNA
0	0	0	9	17	NM_079899.3	CG11186-RA (toy), mRNA
0	0	0	9	6	NM_137912.1	CG9876-RA (CG9876), mRNA
0	0	0	8	27	NM_134474.4	CG32532-RA (CG32532), mRNA
0	0	0	8	10	NM_168497.1	CG32105-RB (CG32105), mRNA
0	0	0	7	10	NM_206250.2	CG16973-RC, transcript variant C (msn), mRNA

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