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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3922R-1 |
| Symbol |
RpS17 |
Full Name |
Ribosomal protein S17 |
| CG No |
CG3922 |
Old CG No |
CG3922 |
| Synonyms |
M(3)i(55), CG3922, M(3)RpS17, RPS17, M(3)67C, M(3)i[55], D9, M(3)i, M(3)67, M(3L)i[55], unnamed, M(3L)i, rpS17, l(3)dtOA4, l(3)67BDo, anon-EST:Posey48, M(3)q, M(3)S33, BcDNA:RE44119, RpS17 |
| Accession No (Link to NCBI) |
NM_079278.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Akai N, Igaki T, Ohsawa S.
Wingless signaling regulates winner/loser status in Minute cell competition.
Genes Cells (2018) 23(3) 234-240
[
PubMed ID = 29431244
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGGGTCGCGTACGAACCAAG |
| Primer Seq. 3' |
aatctagaggtaccCGACGACCAAAGTTGTTGGTG |
| Predicted Fragment Size |
389 |
| IR fragment full Seq |
0001 ATGGGTCGCG TACGAACCAA GACGGTGAAG AAGGCCGCTA AGGTCATCAT CGAGAAGTAC
0061 TACACTCGCC TGACGTTGGA CTTCCACACC AACAAGCGCA TCTGCGAGGA GGTGGCCATC
0121 ATCCCCACCA ANGCCCCTGC GCAACAAGAT TGCCGGCTAT GTCACCCATT TGATGGGCCG
0181 CCTGCGTCAC TCCCAGGTGC GTGGTATCTC CATCAAGTTG CAGGAGGAGG AGCGTGAGCG
0241 TCGTGACANA CTACGTCCCG GCCGTCTCCG CTCTGGAGCA GGACATCATC GAGGTCGACG
0301 CCGACACCAA GGAGATGTTG AAGCTTCTGG ACTTCCACAA CATCCGTGGC CTGCAGCTCA
0361 CCCAGCCCAA CACCAACAAC TTTGGTCGTC G |
| in silico PCR Fragment |
0001 ATGGGTCGCG TACGAACCAA GACGGTGAAG AAGGCCGCTA AGGTCATCAT CGAGAAGTAC
0061 TACACTCGCC TGACGTTGGA CTTCCACACC AACAAGCGCA TCTGCGAGGA GGTGGCCATC
0121 ATTCCCACCA A-GCCCCTGC GCAACAAGAT TGCCGGCTAT GTCACCCATT TGATGGGCCG
0181 CCTGCGTCAC TCCCAGGTGC GTGGTATCTC CATCAAGTTG CAGGAGGAGG AGCGTGAGCG
0241 TCGTGACA-A CTACGTCCCG GCCGTCTCCG CTCTGGAGCA GGACATCATC GAGGTCGACG
0301 CCGACACCAA GGAGATGTTG AAGCTTCTGG ACTTCCACAA CATCCGTGGC CTGCAGCTCA
0361 CCCAGCCCAA CACCAACAAC TTTGGTCGTC G |
| Assemble Data |
3922R-1.IR_full 1 ATGGGTCGCGTACGAACCAAGACGGTGAAGAAGGCCGCTAAGGTCATCATCGAGAAGTAC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3922R-1.in silico 1 ATGGGTCGCGTACGAACCAAGACGGTGAAGAAGGCCGCTAAGGTCATCATCGAGAAGTAC 60
3922R-1.IR_full 61 TACACTCGCCTGACGTTGGACTTCCACACCAACAAGCGCATCTGCGAGGAGGTGGCCATC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3922R-1.in silico 61 TACACTCGCCTGACGTTGGACTTCCACACCAACAAGCGCATCTGCGAGGAGGTGGCCATC 120
3922R-1.IR_full 121 ATCCCCACCAANGCCCCTGCGCAACAAGATTGCCGGCTATGTCACCCATTTGATGGGCCG 180
|| |||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
3922R-1.in silico 121 ATTCCCACCAA-GCCCCTGCGCAACAAGATTGCCGGCTATGTCACCCATTTGATGGGCCG 180
3922R-1.IR_full 181 CCTGCGTCACTCCCAGGTGCGTGGTATCTCCATCAAGTTGCAGGAGGAGGAGCGTGAGCG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3922R-1.in silico 181 CCTGCGTCACTCCCAGGTGCGTGGTATCTCCATCAAGTTGCAGGAGGAGGAGCGTGAGCG 240
3922R-1.IR_full 241 TCGTGACANACTACGTCCCGGCCGTCTCCGCTCTGGAGCAGGACATCATCGAGGTCGACG 300
|||||||| |||||||||||||||||||||||||||||||||||||||||||||||||||
3922R-1.in silico 241 TCGTGACA-ACTACGTCCCGGCCGTCTCCGCTCTGGAGCAGGACATCATCGAGGTCGACG 300
3922R-1.IR_full 301 CCGACACCAAGGAGATGTTGAAGCTTCTGGACTTCCACAACATCCGTGGCCTGCAGCTCA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3922R-1.in silico 301 CCGACACCAAGGAGATGTTGAAGCTTCTGGACTTCCACAACATCCGTGGCCTGCAGCTCA 360
3922R-1.IR_full 361 CCCAGCCCAACACCAACAACTTTGGTCGTCG 391
|||||||||||||||||||||||||||||||
3922R-1.in silico 361 CCCAGCCCAACACCAACAACTTTGGTCGTCG 391
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
371 |
0 |
0 |
0 |
NM_079278.2 |
CG3922-RB (RpS17), mRNA |
| 0 |
0 |
4 |
4 |
8 |
NM_079176.1 |
CG12008-RA, transcript variant A (kst), mRNA |
| 0 |
0 |
4 |
4 |
8 |
NM_206266.1 |
CG12008-RB, transcript variant B (kst), mRNA |
| 0 |
0 |
4 |
4 |
8 |
NM_206265.1 |
CG12008-RC, transcript variant C (kst), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_142448.2 |
CG8064-RA (CG8064), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_169683.1 |
CG31291-RA, transcript variant A (CG31291), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_169682.1 |
CG31291-RB, transcript variant B (CG31291), mRNA |
| 0 |
0 |
2 |
5 |
6 |
NM_165878.1 |
CG30046-RB (CG30046), mRNA |
| 0 |
0 |
2 |
5 |
4 |
NM_167313.1 |
CG2467-RB, transcript variant B (CG2467), mRNA |
| 0 |
0 |
2 |
5 |
4 |
NM_132540.1 |
CG2467-RA, transcript variant A (CG2467), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_134584.2 |
CG32512-RA (CG32512), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_170536.2 |
CG2048-RB, transcript variant B (dco), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_170535.1 |
CG2048-RA, transcript variant A (dco), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_079863.2 |
CG2048-RC, transcript variant C (dco), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_078539.2 |
CG8971-RA (fh), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_136556.2 |
CG14749-RA (CG14749), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_169394.1 |
CG17230-RB, transcript variant B (CG17230), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_141820.1 |
CG17230-RA, transcript variant A (CG17230), mRNA |
| 0 |
0 |
1 |
9 |
12 |
NM_135304.2 |
CG7154-RA (CG7154), mRNA |
| 0 |
0 |
1 |
7 |
8 |
NM_165915.1 |
CG8815-RB, transcript variant B (Sin3A), mRNA |
| 0 |
0 |
1 |
7 |
8 |
NM_165916.1 |
CG8815-RC, transcript variant C (Sin3A), mRNA |
| 0 |
0 |
1 |
7 |
8 |
NM_136955.2 |
CG8815-RA, transcript variant A (Sin3A), mRNA |
| 0 |
0 |
1 |
7 |
7 |
NM_165917.1 |
CG8815-RD, transcript variant D (Sin3A), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_057268.3 |
CG6944-RA (Lam), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_080170.2 |
CG11156-RA (mus101), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_057505.3 |
CG3644-RA, transcript variant A (bic), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_165954.1 |
CG3644-RB, transcript variant B (bic), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_169644.1 |
CG4843-RB, transcript variant B (Tm2), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_169645.2 |
CG4843-RC, transcript variant C (Tm2), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_079637.4 |
CG4843-RA, transcript variant A (Tm2), mRNA |
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