NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 3909R-3

Symbol CG3909 Full Name CG3909

CG No CG3909 Old CG No CG3909

Synonyms BcDNA:LD21537, CG3909

Accession No (Link to NCBI) NM_141712.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees pupal semi-lethal

Map Viewer

Reference
[Please submit your publication] Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAGAGGAAAATGCGCACGACAG

Primer Seq. 3'

aatctagaggtaccGCGTGTATTTCCCATTTTGGG

Predicted Fragment Size

IR fragment full Seq

0001 AGAGGAAAAT GCGCACGACA GTCAGCTATG GGCGTGCACC TGGGGACGCG ACACTGCGGC 
0061 CTCAGATCCG GATGACGCGG TCGTGGAGCC CGAGGAGAAT CCCTTTGATT TCGATAAGAA 
0121 GGAGGCCCGC CCGAAGGATT TCTTGGTCAC CGGCGGACTG GATGACTTGG TAAAGGTGTG 
0181 GGACTTGCAG GAGGACAACA CCCTAAAGTT GCGCCACAAA CTAAAAGGAC ATGCCCTGGG 
0241 AGTTGTTTCT GTGGCCGTCA GTTCCGATGG ACAGACCATT GCCAGTAGTT CTCTGGACTC 
0301 CACCATGTGT TTGTGGNGAT GCCCGTTCGG GCGACNAAGA AACACCTACT TAGCTTTGGG 
0361 CCTGTTGATC TCTGGACGGT GCAATTTTCA CCCTGCAACA AATATGTGAT ATCCGGACTG 
0421 AACGACGGCA AAATCTCCAT GTACAGTGTG GAAACCGGAA AGGCAGAGCA GACATTGGAT 
0481 GCCCAAAATG GGAAATANCA CGC

in silico PCR Fragment

0001 AGAGGAAAAT GCGCACGACA GTCAGCTATG GGCGTGCACC TGGGGACGCG ACACTGCGGC 
0061 CTCAGATCCG GATGACGCGG TCGTGGAGCC CGAGGAGAAT CCCTTTGATT TCGATAAGAA 
0121 GGAGGCCCGC CCGAAGGATT TCTTGGTCAC CGGCGGACTG GATGACTTGG TAAAGGTGTG 
0181 GGACTTGCAG GAGGACAACA CCCTAAAGTT GCGCCACAAA CTAAAAGGAC ATGCCCTGGG 
0241 AGTTGTTTCT GTGGCCGTCA GTTCCGATGG ACAGACCATT GCCAGTAGTT CTCTGGACTC 
0301 CACCATGTGT TTGTGG-GAT GCCCGTTCGG GCGAC-AAGA AACACCTACT TAGCTTTGGG 
0361 CCTGTTGATC TCTGGACGGT GCAATTTTCA CCCTGCAACA AATATGTGAT ATCCGGACTG 
0421 AACGACGGCA AAATCTCCAT GTACAGTGTG GAAACCGGAA AGGCAGAGCA GACATTGGAT 
0481 GCCCAAAATG GGAAATA-CA CGC

Assemble Data


3909R-3.IR_full       1   AGAGGAAAATGCGCACGACAGTCAGCTATGGGCGTGCACCTGGGGACGCGACACTGCGGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3909R-3.in silico     1   AGAGGAAAATGCGCACGACAGTCAGCTATGGGCGTGCACCTGGGGACGCGACACTGCGGC 60


3909R-3.IR_full       61  CTCAGATCCGGATGACGCGGTCGTGGAGCCCGAGGAGAATCCCTTTGATTTCGATAAGAA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3909R-3.in silico     61  CTCAGATCCGGATGACGCGGTCGTGGAGCCCGAGGAGAATCCCTTTGATTTCGATAAGAA 120


3909R-3.IR_full       121 GGAGGCCCGCCCGAAGGATTTCTTGGTCACCGGCGGACTGGATGACTTGGTAAAGGTGTG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3909R-3.in silico     121 GGAGGCCCGCCCGAAGGATTTCTTGGTCACCGGCGGACTGGATGACTTGGTAAAGGTGTG 180


3909R-3.IR_full       181 GGACTTGCAGGAGGACAACACCCTAAAGTTGCGCCACAAACTAAAAGGACATGCCCTGGG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3909R-3.in silico     181 GGACTTGCAGGAGGACAACACCCTAAAGTTGCGCCACAAACTAAAAGGACATGCCCTGGG 240


3909R-3.IR_full       241 AGTTGTTTCTGTGGCCGTCAGTTCCGATGGACAGACCATTGCCAGTAGTTCTCTGGACTC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3909R-3.in silico     241 AGTTGTTTCTGTGGCCGTCAGTTCCGATGGACAGACCATTGCCAGTAGTTCTCTGGACTC 300


3909R-3.IR_full       301 CACCATGTGTTTGTGGNGATGCCCGTTCGGGCGACNAAGAAACACCTACTTAGCTTTGGG 360
                          |||||||||||||||| |||||||||||||||||| ||||||||||||||||||||||||
3909R-3.in silico     301 CACCATGTGTTTGTGG-GATGCCCGTTCGGGCGAC-AAGAAACACCTACTTAGCTTTGGG 360


3909R-3.IR_full       361 CCTGTTGATCTCTGGACGGTGCAATTTTCACCCTGCAACAAATATGTGATATCCGGACTG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3909R-3.in silico     361 CCTGTTGATCTCTGGACGGTGCAATTTTCACCCTGCAACAAATATGTGATATCCGGACTG 420


3909R-3.IR_full       421 AACGACGGCAAAATCTCCATGTACAGTGTGGAAACCGGAAAGGCAGAGCAGACATTGGAT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3909R-3.in silico     421 AACGACGGCAAAATCTCCATGTACAGTGTGGAAACCGGAAAGGCAGAGCAGACATTGGAT 480


3909R-3.IR_full       481 GCCCAAAATGGGAAATANCACGC 503
                          ||||||||||||||||| |||||
3909R-3.in silico     481 GCCCAAAATGGGAAATA-CACGC 503

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	2	NM_141712.2	CG3909-RA (CG3909), mRNA
0.41	2	3	3	5	NM_134964.1	CG3921-RA (CG3921), mRNA
0	0	4	2	4	NM_001014605.1	CG1119-RB, transcript variant B (Gnf1), mRNA
0	0	4	2	4	NM_079505.2	CG1119-RA, transcript variant A (Gnf1), mRNA
0	0	3	7	5	NM_140733.2	CG6322-RA (CG6322), mRNA
0	0	3	2	11	NM_132479.2	CG1597-RA (CG1597), mRNA
0	0	2	2	5	NM_135123.2	CG9021-RA (CG9021), mRNA
0	0	1	5	5	NM_132600.1	CG3812-RA, transcript variant A (CG3812), mRNA
0	0	1	5	5	NM_167337.1	CG3812-RB, transcript variant B (CG3812), mRNA
0	0	1	5	4	NM_141571.2	CG8223-RA (CG8223), mRNA
0	0	1	4	3	NM_078770.1	CG11330-RA (cort), mRNA
0	0	1	4	3	NM_132302.3	CG7055-RA (dalao), mRNA
0	0	1	3	5	NM_136736.1	CG12900-RA (CG12900), mRNA
0	0	1	3	3	NM_140370.1	CG11274-RA (SRm160), mRNA
0	0	1	2	5	NM_170152.1	CG13624-RD, transcript variant D (CG13624), mRNA
0	0	1	2	5	NM_170151.1	CG13624-RB, transcript variant B (CG13624), mRNA
0	0	1	2	5	NM_001043287.1	CG13624-RE, transcript variant E (CG13624), mRNA
0	0	1	2	5	NM_170150.1	CG13624-RA, transcript variant A (CG13624), mRNA
0	0	1	2	5	NM_001043288.1	CG13624-RF, transcript variant F (CG13624), mRNA
0	0	1	2	5	NM_143014.2	CG13624-RC, transcript variant C (CG13624), mRNA
0	0	0	5	4	NM_078513.2	CG4626-RA, transcript variant A (fz4), mRNA
0	0	0	5	4	NM_167119.2	CG4626-RB, transcript variant B (fz4), mRNA
0	0	0	4	13	NM_144448.2	CG1915-RC, transcript variant C (sls), mRNA
0	0	0	4	9	NM_079937.2	CG1915-RA, transcript variant A (sls), mRNA
0	0	0	4	7	NM_143459.1	CG1964-RA (Kul), mRNA
0	0	0	4	6	NM_080110.2	CG32356-RA, transcript variant A (ImpE1), mRNA
0	0	0	4	4	NM_078702.1	CG2071-RA (Ser6), mRNA
0	0	0	4	3	NM_141967.2	CG6802-RA (Cyp313a4), mRNA
0	0	0	4	3	NM_142329.2	CG3590-RA (CG3590), mRNA
0	0	0	4	3	NM_132545.2	CG1806-RA (CG1806), mRNA

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