NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 3907R-1

Symbol CG3907 Full Name CG3907

CG No CG3907 Old CG No CG3907

Synonyms CG3907

Accession No (Link to NCBI) NM_166647.2

Inserted Chr. lll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGGACTGCCACCATCGCATTG

Primer Seq. 3'

aatctagaggtaccGCTAAAGTTGTTCCGCAGGCT

Predicted Fragment Size

IR fragment full Seq

0001 TGGACTGCCA CCATCGCATT GAAGTGCCCC TGCgGGCCGG NAAAGAAAAT GAAGAAGCGT 
0061 CGGGAGCTGG ACGCCCTGAT AGCCTCCCAG TCGTCCTCGG GCAAGAGAGC CAACCGGAGA 
0121 GTCCCCGGAG GCACCAACTC CCAGGACAAG CTGCTCTCCG TCTCGACCGA CGACCCTGAG 
0181 GATTACGCAT GGACAAATGT CGCTGGCAGT CGcCGCAGCC ATCGAAAAAT AGCCTATTTC 
0241 CGCATGTgNG GCCCGCTNTC TATTCGGCAT TATGGGCATT CTGATTGTGG GCATTTTGTA 
0301 CTGGCTCTAC TTCGATCTGC GCCAGCAACT CACCGACTAT CGCCAGAAGA TCGAGGAGGT 
0361 GTCTGCCATG AGCAAGAACT TCCCGGACAC CCTGCAAAGA TGGCACGAGA CTTCGTCCTA 
0421 CCTGCTGAAG AACCAAACAG CCGTCATTTC GGAAATAAAT GATTTGCAAA AATCAATGGA 
0481 GAGCCTGCGG AACAACTTTA GC

in silico PCR Fragment

0001 TGGACTGCCA CCATCGCATT GAAGTGCCCC TGCGGGCCGG -AAAGAAAAT GAAGAAGCGT 
0061 CGGGAGCTGG ACGCCCTGAT AGCCTCCCAG TCGTCCTCGG GCAAGAGAGC CAACCGGAGA 
0121 GTCCCCGGAG GCACCAACTC CCAGGACAAG CTGCTCTCCG TCTCGACCGA CGACCCTGAG 
0181 GATTACGCAT GGACAAATGT CGCTGGCAGT CGCCGCAGCC ATCGAAAAAT AGCCTATTTC 
0241 CGCATGTGCG GCCCGCT-TC TATTCGGCAT TATGGGCATT CTGATTGTGG GAATTTTGTA 
0301 CTGGCTCTAC TTCGATCTGC GCCAGCAACT CACCGACTAT CGCCAGAAGA TCGAGGAGGT 
0361 GTCTGCCATG AGCAAGAACT TCCCGGACAC CCTGCAAAGA TGGCACGAGA CTTCGTCCTA 
0421 CCTGCTGAAG AACCAAACAG CCGTCATTTC GGAAATAAAT GATTTGCAAA AATCAATGGA 
0481 GAGCCTGCGG AACAACTTTA GC

Assemble Data


3907R-1.IR_full       1   TGGACTGCCACCATCGCATTGAAGTGCCCCTGCGGGCCGGNAAAGAAAATGAAGAAGCGT 60
                          |||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
3907R-1.in silico     1   TGGACTGCCACCATCGCATTGAAGTGCCCCTGCGGGCCGG-AAAGAAAATGAAGAAGCGT 60


3907R-1.IR_full       61  CGGGAGCTGGACGCCCTGATAGCCTCCCAGTCGTCCTCGGGCAAGAGAGCCAACCGGAGA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3907R-1.in silico     61  CGGGAGCTGGACGCCCTGATAGCCTCCCAGTCGTCCTCGGGCAAGAGAGCCAACCGGAGA 120


3907R-1.IR_full       121 GTCCCCGGAGGCACCAACTCCCAGGACAAGCTGCTCTCCGTCTCGACCGACGACCCTGAG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3907R-1.in silico     121 GTCCCCGGAGGCACCAACTCCCAGGACAAGCTGCTCTCCGTCTCGACCGACGACCCTGAG 180


3907R-1.IR_full       181 GATTACGCATGGACAAATGTCGCTGGCAGTCGCCGCAGCCATCGAAAAATAGCCTATTTC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3907R-1.in silico     181 GATTACGCATGGACAAATGTCGCTGGCAGTCGCCGCAGCCATCGAAAAATAGCCTATTTC 240


3907R-1.IR_full       241 CGCATGTGNGGCCCGCTNTCTATTCGGCATTATGGGCATTCTGATTGTGGGCATTTTGTA 300
                          |||||||| |||||||| ||||||||||||||||||||||||||||||||| ||||||||
3907R-1.in silico     241 CGCATGTGCGGCCCGCT-TCTATTCGGCATTATGGGCATTCTGATTGTGGGAATTTTGTA 300


3907R-1.IR_full       301 CTGGCTCTACTTCGATCTGCGCCAGCAACTCACCGACTATCGCCAGAAGATCGAGGAGGT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3907R-1.in silico     301 CTGGCTCTACTTCGATCTGCGCCAGCAACTCACCGACTATCGCCAGAAGATCGAGGAGGT 360


3907R-1.IR_full       361 GTCTGCCATGAGCAAGAACTTCCCGGACACCCTGCAAAGATGGCACGAGACTTCGTCCTA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3907R-1.in silico     361 GTCTGCCATGAGCAAGAACTTCCCGGACACCCTGCAAAGATGGCACGAGACTTCGTCCTA 420


3907R-1.IR_full       421 CCTGCTGAAGAACCAAACAGCCGTCATTTCGGAAATAAATGATTTGCAAAAATCAATGGA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3907R-1.in silico     421 CCTGCTGAAGAACCAAACAGCCGTCATTTCGGAAATAAATGATTTGCAAAAATCAATGGA 480


3907R-1.IR_full       481 GAGCCTGCGGAACAACTTTAGC 502
                          ||||||||||||||||||||||
3907R-1.in silico     481 GAGCCTGCGGAACAACTTTAGC 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_138026.3	CG3907-RB, transcript variant B (CG3907), mRNA
100	482	0	0	0	NM_166647.2	CG3907-RA, transcript variant A (CG3907), mRNA
0.41	2	2	2	4	NM_142717.2	CG15498-RA (CG15498), mRNA
0	0	2	4	5	NM_170031.1	CG5248-RA, transcript variant A (loco), mRNA
0	0	2	4	5	NM_170030.1	CG5248-RD, transcript variant D (loco), mRNA
0	0	2	4	3	NM_168076.1	CG1318-RD, transcript variant D (Hexo1), mRNA
0	0	2	4	3	NM_079200.4	CG1318-RA, transcript variant A (Hexo1), mRNA
0	0	2	4	3	NM_168075.1	CG1318-RB, transcript variant B (Hexo1), mRNA
0	0	2	4	3	NM_168077.1	CG1318-RC, transcript variant C (Hexo1), mRNA
0	0	2	4	2	NM_139473.2	CG16985-RA (CG16985), mRNA
0	0	2	3	3	NM_131951.1	CG15473-RA (CG15473), mRNA
0	0	1	6	5	NM_130614.2	CG4322-RA (CG4322), mRNA
0	0	1	4	3	NM_140625.2	CG4818-RA (CG4818), mRNA
0	0	1	3	2	NM_132691.2	CG11130-RA (Rtc1), mRNA
0	0	1	2	3	NM_132996.2	CG5675-RA (X11L), mRNA
0	0	1	2	2	NM_132429.2	CG2202-RA (CG2202), mRNA
0	0	0	8	6	NM_137004.2	CG4646-RA (CG4646), mRNA
0	0	0	5	9	NM_141666.2	CG9492-RA (CG9492), mRNA
0	0	0	5	6	NM_136711.2	CG11866-RA (CG11866), mRNA
0	0	0	5	5	NM_137801.2	CG6203-RA, transcript variant A (Fmr1), mRNA
0	0	0	5	5	NM_169324.1	CG6203-RC, transcript variant C (Fmr1), mRNA
0	0	0	5	5	NM_169325.1	CG6203-RD, transcript variant D (Fmr1), mRNA
0	0	0	5	5	NM_169326.1	CG6203-RE, transcript variant E (Fmr1), mRNA
0	0	0	5	5	NM_169327.1	CG6203-RB, transcript variant B (Fmr1), mRNA
0	0	0	5	3	NM_205948.1	CG15828-RB, transcript variant B (CG15828), mRNA
0	0	0	5	3	NM_164538.2	CG31776-RA (CG31776), mRNA
0	0	0	5	3	NM_135460.1	CG15828-RA, transcript variant A (CG15828), mRNA
0	0	0	4	9	NM_080325.3	CG6450-RC (lva), mRNA
0	0	0	4	7	NM_079403.3	CG6292-RB, transcript variant B (CycT), mRNA
0	0	0	4	7	NM_206395.2	CG6292-RA, transcript variant A (CycT), mRNA

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