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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3897R-3 |
| Symbol |
blot |
Full Name |
bloated tubules |
| CG No |
CG3897 |
Old CG No |
CG3897 |
| Synonyms |
l(3)1658, CG3897, blot |
| Accession No (Link to NCBI) |
NM_170637.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGACACGAAATGCTCGGTGTT |
| Primer Seq. 3' |
aatctagaggtaccAAAGGCCTATAAGTCCCGCTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGACACGAAA TGCTCGGTGT TCCGCGGGCT CGTGCTCTGT CTGTGCCTGA ACCTGAGCTA
0061 TGCGAATCGT GGTCCGCTTC CCACGCGAGT TGGACCGCTA CGGGTCGGCG TACCTGGTTC
0121 CGTACGTGGT GTTGCTGTTT CTAGTCGGCC TGCCCATGGT TCTGCTGGAG ATCTCGGTGG
0181 GCCAGTTTCT GGGCCAGGGG GCGgcGCGAC ACCTgGCGCG CCTCGCCCAT TTTCAAAGGA
0241 GCCTGCATGA TCAGTCNGCT TTGCCTCCTG GCTTTCGGCC ATTTGGGTGT CCTTGCAGGC
0301 CGTGCTGGCA CTGGCCTACA TCGGGATGTT CGCCTCCAAT GACCTGCCAT TCCGGGAGTG
0361 CGCTGGACCC GTGAAGCTGC GATTGAGTGG ATACNTCCTG ACGGGAACCA GTGGACAAGA
0421 GTGTTTGCAG CTGACCTTTC TAACTNCATT CTGGCGGAAT CCTCTGTACT TTGGTCTTCT
0481 GGCAGCGGGA CTTATAGGCC TTT |
| in silico PCR Fragment |
0001 TGACACGAAA TGCTCGGTGT TCCGCGGGCT CGTGCTCTGT CTGTGCCTGA ACCTGAGCTA
0061 TGCGAA-CGT GGTCCGCTTC CCACGCGAGT TGGACCGCTA CGGGTCGGCG TACCTGGTTC
0121 CGTACGTGGT GTTGCTGTTT CTAGTCGGCC TGCCCATGGT TCTGCTGGAG ATCTCGGTGG
0181 GCCAGTTTCT GGGCCAGGGG GCGGCGC-AC ACCTGGCGCG CCTCGCCCAT TTTCAAAGGA
0241 GCCTGCATGA TCAGTC-GCT TTGCCTCCTG GCTTTCGGCC ATTTGGGTGT CCTTGCAGGC
0301 CGTGCTGGCA CTGGCCTACA TCGGGATGTT CGCCTCCAAT GACCTGCCAT TCCGGGAGTG
0361 CGCTGGACCC GTGAAGCTGC GATTGAGTGG ATACCTCCTG ACGGGAACCA GTGGACAAGA
0421 GTGTTTGCAG CTGACCTTTC TAACTCCATT CTGGCGGAAT CCTCTGTACT TTGGTCTTCT
0481 GGCAGCGGGA CTTATAGGCC TTT |
| Assemble Data |
3897R-3.IR_full 1 TGACACGAAATGCTCGGTGTTCCGCGGGCTCGTGCTCTGTCTGTGCCTGAACCTGAGCTA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3897R-3.in silico 1 TGACACGAAATGCTCGGTGTTCCGCGGGCTCGTGCTCTGTCTGTGCCTGAACCTGAGCTA 60
3897R-3.IR_full 61 TGCGAATCGTGGTCCGCTTCCCACGCGAGTTGGACCGCTACGGGTCGGCGTACCTGGTTC 120
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
3897R-3.in silico 61 TGCGAA-CGTGGTCCGCTTCCCACGCGAGTTGGACCGCTACGGGTCGGCGTACCTGGTTC 120
3897R-3.IR_full 121 CGTACGTGGTGTTGCTGTTTCTAGTCGGCCTGCCCATGGTTCTGCTGGAGATCTCGGTGG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3897R-3.in silico 121 CGTACGTGGTGTTGCTGTTTCTAGTCGGCCTGCCCATGGTTCTGCTGGAGATCTCGGTGG 180
3897R-3.IR_full 181 GCCAGTTTCTGGGCCAGGGGGCGGCGCGACACCTGGCGCGCCTCGCCCATTTTCAAAGGA 240
||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
3897R-3.in silico 181 GCCAGTTTCTGGGCCAGGGGGCGGCGC-ACACCTGGCGCGCCTCGCCCATTTTCAAAGGA 240
3897R-3.IR_full 241 GCCTGCATGATCAGTCNGCTTTGCCTCCTGGCTTTCGGCCATTTGGGTGTCCTTGCAGGC 300
|||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
3897R-3.in silico 241 GCCTGCATGATCAGTC-GCTTTGCCTCCTGGCTTTCGGCCATTTGGGTGTCCTTGCAGGC 300
3897R-3.IR_full 301 CGTGCTGGCACTGGCCTACATCGGGATGTTCGCCTCCAATGACCTGCCATTCCGGGAGTG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3897R-3.in silico 301 CGTGCTGGCACTGGCCTACATCGGGATGTTCGCCTCCAATGACCTGCCATTCCGGGAGTG 360
3897R-3.IR_full 361 CGCTGGACCCGTGAAGCTGCGATTGAGTGGATACNTCCTGACGGGAACCAGTGGACAAGA 420
|||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
3897R-3.in silico 361 CGCTGGACCCGTGAAGCTGCGATTGAGTGGATACCTCCTGACGGGAACCAGTGGACAAGA 420
3897R-3.IR_full 421 GTGTTTGCAGCTGACCTTTCTAACTNCATTCTGGCGGAATCCTCTGTACTTTGGTCTTCT 480
||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
3897R-3.in silico 421 GTGTTTGCAGCTGACCTTTCTAACTCCATTCTGGCGGAATCCTCTGTACTTTGGTCTTCT 480
3897R-3.IR_full 481 GGCAGCGGGACTTATAGGCCTTT 503
|||||||||||||||||||||||
3897R-3.in silico 481 GGCAGCGGGACTTATAGGCCTTT 503
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_170637.1 |
CG3897-RA, transcript variant A (blot), mRNA |
| 99.17 |
478 |
2 |
1 |
1 |
NM_170636.1 |
CG3897-RD, transcript variant D (blot), mRNA |
| 99.17 |
478 |
2 |
1 |
1 |
NM_079401.2 |
CG3897-RB, transcript variant B (blot), mRNA |
| 99.17 |
478 |
2 |
1 |
1 |
NM_170638.1 |
CG3897-RC, transcript variant C (blot), mRNA |
| 0 |
0 |
3 |
2 |
8 |
NM_001042862.1 |
CG7337-RC, transcript variant C (CG7337), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_164996.1 |
CG31760-RA (CG31760), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_175960.3 |
CG33196-RB (dp), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_139714.3 |
CG10624-RA (sinu), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_136120.1 |
CG13085-RA (CG13085), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_206499.1 |
CG8956-RD, transcript variant D (Ahcy89E), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_206500.1 |
CG8956-RC, transcript variant C (Ahcy89E), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_144020.1 |
CG18673-RA (CG18673), mRNA |
| 0 |
0 |
0 |
6 |
10 |
NM_136261.2 |
CG18362-RB, transcript variant B (Mio), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_001031966.1 |
CG33710-RA (CG33710), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_176195.1 |
CG33017-RB (CG33017), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_132301.3 |
CG7065-RA (CG7065), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_141455.2 |
CG1227-RA (CG1227), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_079670.2 |
CG7223-RB, transcript variant B (htl), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_169784.1 |
CG7223-RA, transcript variant A (htl), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_169785.1 |
CG7223-RC, transcript variant C (htl), mRNA |
| 0 |
0 |
0 |
3 |
14 |
NM_137545.1 |
CG18605-RA (CG18605), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_141738.3 |
CG6303-RA (Bruce), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_132264.2 |
CG2056-RA, transcript variant A (CG2056), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_167161.1 |
CG2056-RB, transcript variant B (CG2056), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_205943.1 |
CG33298-RA, transcript variant A (CG33298), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_205944.1 |
CG33298-RB, transcript variant B (CG33298), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_140901.2 |
CG7762-RA (Rpn1), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_134924.1 |
CG3327-RB, transcript variant B (E23), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_079909.2 |
CG3327-RA, transcript variant A (E23), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_205900.1 |
CG3327-RC, transcript variant C (E23), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
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|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| ++ |
|
| DC |
 |
| + |
|
| DM |
 |
| ++ |
|
| VM |
 |
| + |
|
| VB |
 |
| |
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