NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 3891R-1

Symbol CG3891 Full Name CG3891

CG No CG3891 Old CG No CG3891

Synonyms CG3891

Accession No (Link to NCBI) NM_140056.2

Inserted Chr. ll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]

Mummery-Widmer JL, Yamazaki M, Stoeger T, Novatchkova M, Bhalerao S, Chen D, Dietzl G, Dickson BJ, Knoblich JA.
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.
Nature (2009) 458(7241) 987-92 [ PubMed ID = 19363474 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGATACCAATGCCCATGCTGC

Primer Seq. 3'

aatctagaggtaccCTGCTGGTTTTGGGTCTGGGA

Predicted Fragment Size

IR fragment full Seq

0001 TGATACCAAT GCCCATGCTG CCCACCGGAG CGGCGCAAAT CATAATTGGC CAGCAGCCAC 
0061 AGGGTCAGGC GACCGCGACG GGTCTGCAGC CACAATTGAT ACCGCTGCAG GCCAACCAGA 
0121 TTATGCTGNC AGGCTGCGCA GCAGCAGCCA CAGNATGCAG GTGATGCAGC TGCCCNATGG 
0181 CCAGACTATA TTCTATCAGA CGCCCACCAT TGCCGCCTTG GATCCGANAT GCAGCGGCCA 
0241 ACGCCNGCag CTGCCNATGG CANGCCCAGC CGACGCCGCA CTACCTCAAT ATCAATGGGC 
0301 AGCTGGTGCA GATCAATCCA GCACCGAGCG CTAATCAGGC GGCACCCACA GCTGGTCAAC 
0361 AGATTATAAT GGTGCCGCAG ACAGCGATGG CAGCGGTGAA TGCAGCGGCC GCCAATGCCG 
0421 GAGTAGGCGC CGGAGTGGGC ACAGTGGTTA CCCAACAACA ACAGCAGCAG CATCAACAAG 
0481 TACAATCCCA GACCCAAAAC CAGCAG

in silico PCR Fragment

0001 TGATACCAAT GCCCATGCTG CCCACCGGAG CGGCGCAAAT CATAATTGGC CAGCAGCCAC 
0061 AGGGTCAGGC GACGGCGACG GGTCTGCAGC CACAATTGAT ACCGCTGCAG GCCAACCAGA 
0121 TTATGCTG-C AGGCTGCGCA GCAGCAGCCA CAG-ATGCAG GTGATGCAGC TGCCCGATGG 
0181 CCAGACTATA TTCTATCAGA CGCCCACCAT TGCCGCCTTG GATCCGA-AT GCAGCGGCCA 
0241 ACGCC-GCAG CTGCC-ATGG CA-GCCCAGC CGACGCCGCA CTACCTCAAT ATCAATGGGC 
0301 AGCTGGTGCA GATCAATCCA GCACCGAGCG CTAATCAGGC GGCACCCACA GCTGGGCAAC 
0361 AGATTATAAT GGTGCCGCAG ACAGCGATGG CAGCGGTGAA TGCAGCGGCC GCCAATGCCG 
0421 GAGTAGGCGC CGGAGTGGGC ACAGTGGTTA CCCAACAACA ACAGCAGCAG CATCAACAAG 
0481 TACAATCCCA GACCCAAAAC CAGCAG

Assemble Data


3891R-1.IR_full       1   TGATACCAATGCCCATGCTGCCCACCGGAGCGGCGCAAATCATAATTGGCCAGCAGCCAC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3891R-1.in silico     1   TGATACCAATGCCCATGCTGCCCACCGGAGCGGCGCAAATCATAATTGGCCAGCAGCCAC 60


3891R-1.IR_full       61  AGGGTCAGGCGACCGCGACGGGTCTGCAGCCACAATTGATACCGCTGCAGGCCAACCAGA 120
                          ||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||
3891R-1.in silico     61  AGGGTCAGGCGACGGCGACGGGTCTGCAGCCACAATTGATACCGCTGCAGGCCAACCAGA 120


3891R-1.IR_full       121 TTATGCTGNCAGGCTGCGCAGCAGCAGCCACAGNATGCAGGTGATGCAGCTGCCCNATGG 180
                          |||||||| |||||||||||||||||||||||| ||||||||||||||||||||| ||||
3891R-1.in silico     121 TTATGCTG-CAGGCTGCGCAGCAGCAGCCACAG-ATGCAGGTGATGCAGCTGCCCGATGG 180


3891R-1.IR_full       181 CCAGACTATATTCTATCAGACGCCCACCATTGCCGCCTTGGATCCGANATGCAGCGGCCA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
3891R-1.in silico     181 CCAGACTATATTCTATCAGACGCCCACCATTGCCGCCTTGGATCCGA-ATGCAGCGGCCA 240


3891R-1.IR_full       241 ACGCCNGCAGCTGCCNATGGCANGCCCAGCCGACGCCGCACTACCTCAATATCAATGGGC 300
                          ||||| ||||||||| |||||| |||||||||||||||||||||||||||||||||||||
3891R-1.in silico     241 ACGCC-GCAGCTGCC-ATGGCA-GCCCAGCCGACGCCGCACTACCTCAATATCAATGGGC 300


3891R-1.IR_full       301 AGCTGGTGCAGATCAATCCAGCACCGAGCGCTAATCAGGCGGCACCCACAGCTGGTCAAC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
3891R-1.in silico     301 AGCTGGTGCAGATCAATCCAGCACCGAGCGCTAATCAGGCGGCACCCACAGCTGGGCAAC 360


3891R-1.IR_full       361 AGATTATAATGGTGCCGCAGACAGCGATGGCAGCGGTGAATGCAGCGGCCGCCAATGCCG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3891R-1.in silico     361 AGATTATAATGGTGCCGCAGACAGCGATGGCAGCGGTGAATGCAGCGGCCGCCAATGCCG 420


3891R-1.IR_full       421 GAGTAGGCGCCGGAGTGGGCACAGTGGTTACCCAACAACAACAGCAGCAGCATCAACAAG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3891R-1.in silico     421 GAGTAGGCGCCGGAGTGGGCACAGTGGTTACCCAACAACAACAGCAGCAGCATCAACAAG 480


3891R-1.IR_full       481 TACAATCCCAGACCCAAAACCAGCAG 506
                          ||||||||||||||||||||||||||
3891R-1.in silico     481 TACAATCCCAGACCCAAAACCAGCAG 506

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	10	49	NM_140056.2	CG3891-RA (CG3891), mRNA
2.28	11	12	54	120	NM_135615.2	CG6700-RA (CG6700), mRNA
1.86	9	2	11	25	NM_078614.3	CG8544-RB, transcript variant B (sd), mRNA
1.86	9	2	11	25	NM_167465.1	CG8544-RA, transcript variant A (sd), mRNA
1.86	9	2	11	25	NM_206727.1	CG8544-RC, transcript variant C (sd), mRNA
1.65	8	53	189	466	NM_168571.2	CG32133-RA (CG32133), mRNA
1.24	6	35	133	351	NM_001038734.1	CG16902-RC (Hr4), mRNA
1.24	6	32	127	234	NM_139799.3	CG10107-RA, transcript variant A (CG10107), mRNA
1.24	6	32	127	234	NM_206286.1	CG10107-RC, transcript variant C (CG10107), mRNA
1.24	6	23	121	314	NM_079903.2	CG15319-RB (nej), mRNA
1.24	6	15	69	204	NM_176591.1	CG15532-RC, transcript variant C (hdc), mRNA
1.24	6	15	69	204	NM_079853.2	CG15532-RA, transcript variant A (hdc), mRNA
1.03	5	13	50	73	NM_057520.3	CG3497-RA (Su(H)), mRNA
0.82	4	47	335	937	NM_167335.1	CG4013-RC, transcript variant C (Smr), mRNA
0.82	4	47	335	937	NM_167334.1	CG4013-RB, transcript variant B (Smr), mRNA
0.82	4	47	335	937	NM_080536.2	CG4013-RA, transcript variant A (Smr), mRNA
0.82	4	34	97	214	NM_137690.2	CG10543-RA, transcript variant A (CG10543), mRNA
0.82	4	34	95	193	NM_176241.1	CG10543-RC, transcript variant C (CG10543), mRNA
0.82	4	34	95	193	NM_166416.1	CG10543-RB, transcript variant B (CG10543), mRNA
0.82	4	33	86	204	NM_168179.1	CG32394-RA (CG32394), mRNA
0.82	4	27	92	264	NM_078797.2	CG13109-RA (tai), mRNA
0.82	4	22	73	159	NM_168413.1	CG32062-RB, transcript variant B (CG32062), mRNA
0.82	4	22	73	159	NM_168412.1	CG32062-RD, transcript variant D (CG32062), mRNA
0.82	4	22	46	108	NM_141639.1	CG16779-RA (CG16779), mRNA
0.82	4	20	65	92	NM_057489.3	CG4722-RA (bib), mRNA
0.82	4	19	77	169	NM_078523.2	CG2252-RB, transcript variant B (fs(1)h), mRNA
0.82	4	17	128	215	NM_139493.2	CG2083-RA (CG2083), mRNA
0.82	4	17	68	180	NM_137212.2	CG30089-RA (CG30089), mRNA
0.82	4	16	55	142	NM_168237.1	CG17888-RD, transcript variant D (Pdp1), mRNA
0.82	4	16	30	101	NM_079736.2	CG10868-RA, transcript variant A (orb), mRNA

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