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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 3884R-3 
 Symbol CG3884  Full Name CG3884 
 CG No CG3884  Old CG No CG3884 
 Synonyms CG3884 
 Accession No (Link to NCBI) NM_165965.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees male lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGAGGGCGATATGTTGGTCTGC 
 Primer Seq. 3' 
aatctagaggtaccGCCGGAGGCCTGTCAGGAAAT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 gNGGGCGATT ATGTTGGTCT GCAAGGTGGT GCCGAGCAAG CAACTGGGAT TCATTTCGCA 
0061 GCGAGGAGAG GCACTGCCCA AGGACATCTT CGAGGTGCTG TGCGGTCAGA ATCTGGTGTG 
0121 GATCAAGTGC TACGATCATG TGATTCCGGA GAATGCTGTA CTCTGTGGAC GCACATCGCT 
0181 GGATCAGCCG GTTTACATTG GTCGTGGTCA TTATGAGGGT CATCTGATTA TCGGCAAAAT 
0241 CTCCTCTGTC CATCGAGCTT TGTTCATCGC TTTTCGCGGT GCCGAACGTC GTCTGGATTC 
0301 CTATGAGATT TTGGTGGAGG AGCGCAGAGT GGTTCCTGGC TGGCAGCTGN CGCCACCACC 
0361 TCCTCCACTA GAGGAGCCGG AAAAGTGTCC ACTATCGCCG CCGCCACACA CCTATGNTCT 
0421 CCACCGGCAA TGGCGCCGNC ACTGCCGGCC AAACCATATC CATATCCGGA TTTGGCTGCG 
0481 ATGCCATTTC CTGANCAGGC CtCNGGC 
 in silico PCR Fragment 
0001 GAGGGCGA-T ATGTTGGTCT GCAAGGTGGT GCCGAGCAAG CAACTGGGAT TCATTTCGCA 
0061 GCGAGGAGAG GCACTGCCCA AGGACATCTT CGAGGTGCTG TGCGGTCAGA ATCTGGTGTG 
0121 GATCAAGTGC TACGATCATG TGATTCCGGA GAATGCTGTA CTCTGTGGAC GCACATCGCT 
0181 GGATCAGCCG GTTTACATTG GTCGTGGTCA TTATGAGGGT CATCTGATTA TCGGCAAAAT 
0241 CTCCTCTGTC CATCGAGCTT TGTTCATCGC TTTTCGCGGT GCCGAACGTC GTCTGGATTC 
0301 CTATGAGATT TTGGTGGAGG AGCGCAGAGT GGTTCCTGGC TGGCAGCTGC CGCCACCACC 
0361 TCCTCCACTA GAGGAGCCGG AAAAGTGTCC ACTATCGCCG CCGCCAC-CA CCT----TCT 
0421 CCACCGGCAA TGGCGCCGCC ACTGCCGGCC AAACCATATC CATATCCGGA TTTGGCTGCG 
0481 ATGCCATTTC CTGA-CAGGC CTCCGGC 
 Assemble Data 

3884R-3.IR_full       1   GNGGGCGATTATGTTGGTCTGCAAGGTGGTGCCGAGCAAGCAACTGGGATTCATTTCGCA 60
                          | |||||| |||||||||||||||||||||||||||||||||||||||||||||||||||
3884R-3.in silico     1   GAGGGCGA-TATGTTGGTCTGCAAGGTGGTGCCGAGCAAGCAACTGGGATTCATTTCGCA 60


3884R-3.IR_full       61  GCGAGGAGAGGCACTGCCCAAGGACATCTTCGAGGTGCTGTGCGGTCAGAATCTGGTGTG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3884R-3.in silico     61  GCGAGGAGAGGCACTGCCCAAGGACATCTTCGAGGTGCTGTGCGGTCAGAATCTGGTGTG 120


3884R-3.IR_full       121 GATCAAGTGCTACGATCATGTGATTCCGGAGAATGCTGTACTCTGTGGACGCACATCGCT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3884R-3.in silico     121 GATCAAGTGCTACGATCATGTGATTCCGGAGAATGCTGTACTCTGTGGACGCACATCGCT 180


3884R-3.IR_full       181 GGATCAGCCGGTTTACATTGGTCGTGGTCATTATGAGGGTCATCTGATTATCGGCAAAAT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3884R-3.in silico     181 GGATCAGCCGGTTTACATTGGTCGTGGTCATTATGAGGGTCATCTGATTATCGGCAAAAT 240


3884R-3.IR_full       241 CTCCTCTGTCCATCGAGCTTTGTTCATCGCTTTTCGCGGTGCCGAACGTCGTCTGGATTC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3884R-3.in silico     241 CTCCTCTGTCCATCGAGCTTTGTTCATCGCTTTTCGCGGTGCCGAACGTCGTCTGGATTC 300


3884R-3.IR_full       301 CTATGAGATTTTGGTGGAGGAGCGCAGAGTGGTTCCTGGCTGGCAGCTGNCGCCACCACC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
3884R-3.in silico     301 CTATGAGATTTTGGTGGAGGAGCGCAGAGTGGTTCCTGGCTGGCAGCTGCCGCCACCACC 360


3884R-3.IR_full       361 TCCTCCACTAGAGGAGCCGGAAAAGTGTCCACTATCGCCGCCGCCACACACCTATGNTCT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||| |||||    |||
3884R-3.in silico     361 TCCTCCACTAGAGGAGCCGGAAAAGTGTCCACTATCGCCGCCGCCAC-CACCT----TCT 420


3884R-3.IR_full       421 CCACCGGCAATGGCGCCGNCACTGCCGGCCAAACCATATCCATATCCGGATTTGGCTGCG 480
                          |||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
3884R-3.in silico     421 CCACCGGCAATGGCGCCGCCACTGCCGGCCAAACCATATCCATATCCGGATTTGGCTGCG 480


3884R-3.IR_full       481 ATGCCATTTCCTGANCAGGCCTCNGGC 507
                          |||||||||||||| |||||||| |||
3884R-3.in silico     481 ATGCCATTTCCTGA-CAGGCCTCCGGC 507



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  4  8  19  NM_165965.1  CG3884-RA, transcript variant A (CG3884), mRNA 
0.82   4  5  7  8  NM_141998.1  CG11670-RA (CG11670), mRNA 
0.41   2  11  7  17  NM_141646.1  CG16788-RA (RnpS1), mRNA 
0.2   1  5  5  10  NM_165341.1  CG1768-RB, transcript variant B (dia), mRNA 
0.2   1  5  5  10  NM_057633.3  CG1768-RA, transcript variant A (dia), mRNA 
0.2   1  4  9  11  NM_169381.1  CG17184-RB, transcript variant B (CG17184), mRNA 
0.2   1  4  9  11  NM_141801.1  CG17184-RA, transcript variant A (CG17184), mRNA 
0.2   1  3  3  4  NM_001042791.1  CG3019-RF, transcript variant F (su(w[a])), mRNA 
0.2   1  3  3  4  NM_057408.3  CG3019-RA, transcript variant A (su(w[a])), mRNA 
0.2   1  3  3  4  NM_206601.1  CG3019-RB, transcript variant B (su(w[a])), mRNA 
0.2   1  3  3  4  NM_206600.1  CG3019-RC, transcript variant C (su(w[a])), mRNA 
0.2   1  3  3  4  NM_001042792.1  CG3019-RD, transcript variant D (su(w[a])), mRNA 
0.2   1  2  4  7  NM_136986.2  CG3884-RB, transcript variant B (CG3884), mRNA 
0   0  6  10  25  NM_144354.2  CG6987-RA (SF2), mRNA 
0   0  6  8  21  NM_130624.2  CG16903-RA (CG16903), mRNA 
0   0  6  8  10  NM_169947.1  CG17299-RF, transcript variant F (SNF4Agamma), mRNA 
0   0  6  8  9  NM_176254.1  CG5403-RB, transcript variant B (retn), mRNA 
0   0  6  8  9  NM_057516.3  CG5403-RA, transcript variant A (retn), mRNA 
0   0  4  9  38  NM_168254.1  CG7915-RB, transcript variant B (Ect4), mRNA 
0   0  4  9  20  NM_001043129.1  CG7915-RC, transcript variant C (Ect4), mRNA 
0   0  3  18  31  NM_165522.1  CG11112-RB, transcript variant B (CG11112), mRNA 
0   0  3  10  41  NM_079519.2  CG10279-RA, transcript variant A (Rm62), mRNA 
0   0  3  10  41  NM_169121.1  CG10279-RF, transcript variant F (Rm62), mRNA 
0   0  3  10  41  NM_169120.1  CG10279-RC, transcript variant C (Rm62), mRNA 
0   0  3  10  41  NM_169122.1  CG10279-RB, transcript variant B (Rm62), mRNA 
0   0  3  10  41  NM_169119.1  CG10279-RE, transcript variant E (Rm62), mRNA 
0   0  3  10  41  NM_169118.1  CG10279-RD, transcript variant D (Rm62), mRNA 
0   0  3  7  8  NM_143605.2  CG11339-RA (CG11339), mRNA 
0   0  3  4  9  NM_206356.2  CG33261-RB, transcript variant B (Trl), mRNA 
0   0  3  4  9  NM_001038926.1  CG33261-RI, transcript variant I (Trl), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
+ 
DC
+ 
DM
 
VM
 
VB
 
Caspase-3 signal
N
 
DB
++ 
DC
++ 
DM
+ 
VM
 
VB
 
Dcompartment   Penetrance
    - 

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