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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
temporarily unavailable
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3874R-2 |
| Symbol |
frc |
Full Name |
fringe connection |
| CG No |
CG3874 |
Old CG No |
CG3874 |
| Synonyms |
UDP-GlcNAc/UDP-GlcUA, ust74C, UST74C, l(3)10098, l(3)02619, l(3)00073, CG3874, l(3)j2E3, l(3)L6750, l(3)S070006, anon-WO0172774.7, anon-WO0172774.5, frc, FRC |
| Accession No (Link to NCBI) |
|
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
late pharate adult |
| Map Viewer |
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Reference
[Please submit your publication]
|
Yamamoto-Hino M, Yoshida H, Ichimiya T, Sakamura S, Maeda M, Kimura Y, Sasaki N, Aoki-Kinoshita KF, Kinoshita-Toyoda A, Toyoda H, Ueda R, Nishihara S, Goto S.
Phenotype-based clustering of glycosylation-related genes by RNAi-mediated gene silencing.
Genes Cells (2015) 20(6) 521-42
[
PubMed ID = 25940448
]
[
RRC reference
]
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| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGGCGGAGAGCGATGTCGGAAA |
| Primer Seq. 3' |
aatctagaggtaccGAACCGCATTCGAAGGTCGCA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
|
| in silico PCR Fragment |
|
| Assemble Data |
3874R-2.IR full 1 GGCGNAGAGCGATGTCGGAAACAGGGAGCTGGAGGAGAAGATGGGCGGATCGGCGGATCG 60
|||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
3874R-2.in silico 1 GGCGGAGAGCGATGTCGGAAACAGGGAGCTGGAGGAGAAGATGGGCGGATCGGCGGATCG 60
3874R-2.IR full 61 GTCATCGCTGCTCGATGGATCCGGTTCGAAGGAGCTGAGTCACCGGGAACGCGAGGACTC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3874R-2.in silico 61 GTCATCGCTGCTCGATGGATCCGGTTCGAAGGAGCTGAGTCACCGGGAACGCGAGGACTC 120
3874R-2.IR full 121 GGCGTTGTTCGTCAAGAAGATCGGGAGCGCCTTGTTCTATGGCTTGTCCTCCTTCATGAT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3874R-2.in silico 121 GGCGTTGTTCGTCAAGAAGATCGGGAGCGCCTTGTTCTATGGCTTGTCCTCCTTCATGAT 180
3874R-2.IR full 181 TACGGTGGTAAACAAGACGGTGCTTACCTCCTACCACTTCCCCTCGTTCCTGTTCCTCAG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3874R-2.in silico 181 TACGGTGGTAAACAAGACGGTGCTTACCTCCTACCACTTCCCCTCGTTCCTGTTCCTCAG 240
3874R-2.IR full 241 CCTCGGGCAACTTACTGCTAGCATTGTGGTCCTGGGCATGGGCAAGCGCCTGAAATTGGT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3874R-2.in silico 241 CCTCGGGCAACTTACTGCTAGCATTGTGGTCCTGGGCATGGGCAAGCGCCTGAAATTGGT 300
3874R-2.IR full 301 GAACTTTCCCCCTCTGCAGAGGAATACCTTCGCCAAGATCTTTCCGCTGCCACTGATATT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3874R-2.in silico 301 GAACTTTCCCCCTCTGCAGAGGAATACCTTCGCCAAGATCTTTCCGCTGCCACTGATATT 360
3874R-2.IR full 361 TCTGGGAAACATGATGTTTNGACTGGGTGGCACAAAAACCTTGAGTCTGCCCATGTTCGC 420
||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
3874R-2.in silico 361 TCTGGGAAACATGATGTTTGGACTGGGTGGCACAAAAACCTTGAGTCTGCCCATGTTCGC 420
3874R-2.IR full 421 AGCCCTACGACGCTTCTCTATCCTNATNACCATGCTGCTGGAGCTCAAGATCCTGGGACT 480
|||||||||||||||||||||||| || ||||||||||||||||||||||||||||||||
3874R-2.in silico 421 AGCCCTACGACGCTTCTCTATCCTGATGACCATGCTGCTGGAGCTCAAGATCCTGGGACT 480
3874R-2.IR full 481 GCGACCTTCGAATGCGGTTC 500
||||||||||||||||||||
3874R-2.in silico 481 GCGACCTTCGAATGCGGTTC 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079402.2 |
fringe connection CG3874-RA (frc), mRNA |
| 0.41 |
2 |
3 |
2 |
5 |
NM_170629.2 |
CG32352-RC, transcript variant C (CG32352), mRNA |
| 0.41 |
2 |
3 |
2 |
5 |
NM_168279.2 |
CG32352-RA, transcript variant A (CG32352), mRNA |
| 0.41 |
2 |
3 |
2 |
5 |
NM_168278.1 |
CG32352-RB, transcript variant B (CG32352), mRNA |
| 0 |
0 |
4 |
2 |
6 |
NM_132217.2 |
CG10958-RA (CG10958), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_132146.1 |
inx6 CG17063-RA (inx6), mRNA |
| 0 |
0 |
3 |
3 |
9 |
NM_057716.3 |
peanut CG8705-RA, transcript variant A (pnut), mRNA |
| 0 |
0 |
3 |
3 |
9 |
NM_165597.1 |
peanut CG8705-RB, transcript variant B (pnut), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_143564.1 |
CG11313-RA (CG11313), mRNA |
| 0 |
0 |
2 |
6 |
4 |
NM_167278.1 |
Kinesin associated protein 3 CG11759-RA (Kap3), mRNA |
| 0 |
0 |
1 |
4 |
8 |
NM_166630.1 |
CG5594-RD, transcript variant D (CG5594), mRNA |
| 0 |
0 |
1 |
4 |
8 |
NM_166631.1 |
CG5594-RA, transcript variant A (CG5594), mRNA |
| 0 |
0 |
1 |
4 |
8 |
NM_137605.2 |
CG13872-RA (CG13872), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_166313.1 |
Vha100-3 CG30329-RA (Vha100-3), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_167063.1 |
Longevity assurance gene 1 CG3576-RA, transcript variant A (Lag1), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_144269.2 |
Longevity assurance gene 1 CG3576-RB, transcript variant B (Lag1), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_169181.1 |
CG32468-RA (CG32468), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_140897.2 |
Mi-2 CG8103-RA, transcript variant A (Mi-2), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_001014591.1 |
Mi-2 CG8103-RB, transcript variant B (Mi-2), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_169145.1 |
CG15186-RB, transcript variant B (CG15186), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_206436.1 |
CG15186-RC, transcript variant C (CG15186), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_141402.1 |
CG15186-RA, transcript variant A (CG15186), mRNA |
| 0 |
0 |
0 |
7 |
3 |
NM_080369.2 |
Glyceraldehyde 3 phosphate dehydrogenase 1 CG12055-RA, transcript variant A (Gapdh1), mRNA |
| 0 |
0 |
0 |
7 |
3 |
NM_001038847.1 |
Glyceraldehyde 3 phosphate dehydrogenase 1 CG12055-RB, transcript variant B (Gapdh1), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_079067.2 |
coracle CG11949-RA, transcript variant A (cora), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_166338.1 |
coracle CG11949-RD, transcript variant D (cora), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_166337.1 |
coracle CG11949-RB, transcript variant B (cora), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_166336.1 |
coracle CG11949-RC, transcript variant C (cora), mRNA |
| 0 |
0 |
0 |
5 |
10 |
NM_169645.2 |
Tropomyosin 2 CG4843-RC, transcript variant C (Tm2), mRNA |
| 0 |
0 |
0 |
5 |
10 |
NM_169644.1 |
Tropomyosin 2 CG4843-RB, transcript variant B (Tm2), mRNA |
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