NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 3845R-3

Symbol NAT1 Full Name NAT1

CG No CG3845 Old CG No CG3845

Synonyms CG3845, NAT1, dNAT1, l(2)01424

Accession No (Link to NCBI) NM_165961.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Yoshikane N, Nakamura N, Ueda R, Ueno N, Yamanaka S, Nakamura M.
Drosophila NAT1, a homolog of the vertebrate translational regulator NAT1/DAP5/p97, is required for embryonic germband extension and metamorphosis.
Dev Growth Differ (2007) 49(7) 623-34 [ PubMed ID = 17716306 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTCGTCCACCTTCTTACGCCTG

Primer Seq. 3'

aatctagaggtaccTTTATGGGGACGGGGCCTTCG

Predicted Fragment Size

IR fragment full Seq

0001 TCGTCCACCT TCTTACGCCT GCTGATCGCG GTCTGCCGCG ACAAGTTCAA CAACCGACTC 
0061 AAGCGCGACG AGAACGACAA CAGACCTCCG CCTGAAAATG AGGCCGACGA GGAGGAGCGC 
0121 CGCCATTTGG CCAAACAGCG CATGCTCGGC AACGTCAAAT TCATCGGAGA GCTCAACAAG 
0181 CTGGACATGC TGTCGAAGAA CGTGCTGCAT CAGTGCATCA TGGAGCTATT TGACAAGAAG 
0241 AAGAAGCGTA CGGCCGGTAC GCAGGAAATA TGCGAGGACA TGGAATGCCT GGCTCAACTG 
0301 CTCAAGACTT GCGGCAAGAA TCTGGATTCA GAACAGGGCA AAGAGCTGAT GAACCAGTAC 
0361 TTCGAGAAGC TTGAACGTCG GTCAAAATCA TCTGAATATC CACCGCGGAT ACGTTTTATG 
0421 CTAAAGGACG TAATTGAATT GCGCCAGAAC AATTGGGTGC CGCGCAAATT GGGCACCACC 
0481 GAAgGCCCCG TCCCCATAAA

in silico PCR Fragment

0001 TCGTCCACCT TCTTACGCCT GCTGATCGCG GTCTGCCGCG ACAAGTTCAA CAACCGACTC 
0061 AAGCGCGACG AGAACGACAA CAGACCTCCG CCTGAAAATG AGGCCGACGA GGAGGAGCGC 
0121 CGCCATTTGG CCAAACAGCG CATGCTCGGC AACGTCAAAT TCATCGGAGA GCTCAACAAG 
0181 CTGGACATGC TGTCGAAGAA CGTGCTGCAT CAGTGCATCA TGGAGCTATT TGACAAGAAG 
0241 AAGAAGCGTA CGGCCGGTAC GCAGGAAATG TGCGAGGACA TGGAATGCCT GGCTCAACTG 
0301 CTCAAGACTT GCGGCAAGAA TCTGGATTCA GAACAGGGCA AAGAGCTGAT GAACCAGTAC 
0361 TTCGAGAAGC TTGAACGTCG GTCAAAATCA TCTGAATATC CACCGCGGAT ACGTTTTATG 
0421 CTAAAGGACG TAATTGAATT GCGCCAGAAC AATTGGGTGC CGCGCAAATT GGGCACCACC 
0481 GAAGGCCCCG TCCCCATAAA

Assemble Data


3845R-3.IR_full       1   TCGTCCACCTTCTTACGCCTGCTGATCGCGGTCTGCCGCGACAAGTTCAACAACCGACTC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3845R-3.in silico     1   TCGTCCACCTTCTTACGCCTGCTGATCGCGGTCTGCCGCGACAAGTTCAACAACCGACTC 60


3845R-3.IR_full       61  AAGCGCGACGAGAACGACAACAGACCTCCGCCTGAAAATGAGGCCGACGAGGAGGAGCGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3845R-3.in silico     61  AAGCGCGACGAGAACGACAACAGACCTCCGCCTGAAAATGAGGCCGACGAGGAGGAGCGC 120


3845R-3.IR_full       121 CGCCATTTGGCCAAACAGCGCATGCTCGGCAACGTCAAATTCATCGGAGAGCTCAACAAG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3845R-3.in silico     121 CGCCATTTGGCCAAACAGCGCATGCTCGGCAACGTCAAATTCATCGGAGAGCTCAACAAG 180


3845R-3.IR_full       181 CTGGACATGCTGTCGAAGAACGTGCTGCATCAGTGCATCATGGAGCTATTTGACAAGAAG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3845R-3.in silico     181 CTGGACATGCTGTCGAAGAACGTGCTGCATCAGTGCATCATGGAGCTATTTGACAAGAAG 240


3845R-3.IR_full       241 AAGAAGCGTACGGCCGGTACGCAGGAAATATGCGAGGACATGGAATGCCTGGCTCAACTG 300
                          ||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
3845R-3.in silico     241 AAGAAGCGTACGGCCGGTACGCAGGAAATGTGCGAGGACATGGAATGCCTGGCTCAACTG 300


3845R-3.IR_full       301 CTCAAGACTTGCGGCAAGAATCTGGATTCAGAACAGGGCAAAGAGCTGATGAACCAGTAC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3845R-3.in silico     301 CTCAAGACTTGCGGCAAGAATCTGGATTCAGAACAGGGCAAAGAGCTGATGAACCAGTAC 360


3845R-3.IR_full       361 TTCGAGAAGCTTGAACGTCGGTCAAAATCATCTGAATATCCACCGCGGATACGTTTTATG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3845R-3.in silico     361 TTCGAGAAGCTTGAACGTCGGTCAAAATCATCTGAATATCCACCGCGGATACGTTTTATG 420


3845R-3.IR_full       421 CTAAAGGACGTAATTGAATTGCGCCAGAACAATTGGGTGCCGCGCAAATTGGGCACCACC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3845R-3.in silico     421 CTAAAGGACGTAATTGAATTGCGCCAGAACAATTGGGTGCCGCGCAAATTGGGCACCACC 480


3845R-3.IR_full       481 GAAGGCCCCGTCCCCATAAA 500
                          ||||||||||||||||||||
3845R-3.in silico     481 GAAGGCCCCGTCCCCATAAA 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	3	NM_165961.1	CG3845-RA, transcript variant A (l(2)01424), mRNA
100	482	0	0	3	NM_001038854.1	CG3845-RC, transcript variant C (l(2)01424), mRNA
100	482	0	0	3	NM_143770.2	CG3845-RB, transcript variant B (l(2)01424), mRNA
0.41	2	2	3	2	NM_080029.3	CG13761-RB (Bzd), mRNA
0	0	2	3	5	NM_057658.4	CG12019-RA (Cdc37), mRNA
0	0	2	2	3	NM_135384.2	CG13400-RA (D12), mRNA
0	0	2	2	2	NM_078714.5	CG9559-RA, transcript variant A (fog), mRNA
0	0	2	2	2	NM_001038771.2	CG9559-RB, transcript variant B (fog), mRNA
0	0	1	4	5	NM_139493.2	CG2083-RA (CG2083), mRNA
0	0	1	4	4	NM_132239.1	CG1632-RA (CG1632), mRNA
0	0	1	3	5	NM_079205.3	CG7507-RA, transcript variant A (Dhc64C), mRNA
0	0	1	3	4	NM_168103.1	CG7507-RB, transcript variant B (Dhc64C), mRNA
0	0	1	3	4	NM_133012.2	CG32560-RA (CG32560), mRNA
0	0	1	3	3	NM_132612.1	CG4400-RA (CG4400), mRNA
0	0	1	3	3	NM_136135.2	CG10132-RA (CG10132), mRNA
0	0	1	3	2	NM_137277.2	CG7989-RA (l(2)k07824), mRNA
0	0	1	3	2	NM_168742.2	CG6143-RA, transcript variant A (Pep), mRNA
0	0	1	3	2	NM_206396.1	CG6143-RC, transcript variant C (Pep), mRNA
0	0	1	3	2	NM_080119.3	CG6143-RB, transcript variant B (Pep), mRNA
0	0	1	2	12	NM_169134.1	CG31556-RA (CG31556), mRNA
0	0	1	2	6	NM_176575.1	CG33103-RA, transcript variant A (Ppn), mRNA
0	0	1	2	6	NM_176574.1	CG33103-RB, transcript variant B (Ppn), mRNA
0	0	1	2	4	NM_001014500.1	CG33558-RA (CG33558), mRNA
0	0	1	2	4	NM_142044.2	CG9588-RA (CG9588), mRNA
0	0	1	2	4	NM_057773.3	CG7538-RA (Mcm2), mRNA
0	0	1	2	4	NM_142975.2	CG5669-RA (CG5669), mRNA
0	0	1	2	4	NM_001038902.1	CG33993-RA (CG33993), mRNA
0	0	1	2	2	NM_134496.2	CG14215-RA (CG14215), mRNA
0	0	1	2	2	NM_001015230.1	CG40155-PA.3 (CG40155), mRNA
0	0	0	8	6	NM_078762.4	CG9088-RA, transcript variant A (lid), mRNA

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