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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3842R-2 |
| Symbol |
CG3842 |
Full Name |
CG3842 |
| CG No |
CG3842 |
Old CG No |
CG3842 |
| Synonyms |
CG3842 |
| Accession No (Link to NCBI) |
NM_132088.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
semi-lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGACTCTCTGCTTCCGGGGTTT |
| Primer Seq. 3' |
aatctagaggtaccGATCCAGCAGCAGATTCGTGA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GACTCTCTGC TTCCGGGGTT TCTGGGCCTG GGCCGTGCTG ATATTCCTGA TTGTTCTGGG
0061 CATATTGCTC TTCATGTGGC TGCTGCGCAA GTGCATCCAA GGACCGGCCT ACCGGAAGGC
0121 CAATCGCATC GATGGCAAGG TGGTCATCGT CACCGGCTGC AACACGGGCA TTGGCAAGGA
0181 GACGGTCCTC GAGTTGGCCA AGCGGGGTGC CCGCGTNCCT ACATGGCCTG TCGGGATCCC
0241 GGTCGGTGTG AGGCCGCACG ACTGGATATC ATGGATCGCA GTCGCAACCA GCAGCTCTTC
0301 AATCGCACCC TGGATCTGGG CTCCCTGCAG TCGGTGAGAA ATTTCGTGGA GCGCTTCAAG
0361 GCGGAGGAAT CCCGGCTGGA TATACTGATC AATAATGCCG GCGTTATGGC CTGTCCCAGG
0421 ACCCTCACAG CCGATGGCTT TGAGCAGCAG TTCGGTGTCA ATCATCTGGG ACACTTTTTG
0481 CTCACGAATC TGCTGCTGGA TC |
| in silico PCR Fragment |
0001 GACTCTCTGC TTCCGGGGTT TCTGGGCCTG GGCCGTGCTG ATATTCCTGA TTGTTCTGGG
0061 CATATTGCTC TTCATGTGGC TGCTGCGCAA GTGCATCCAA GGACCGGCCT ACCGGAAGGC
0121 CAATCGCATC GATGGCAAGG TGGTCATCGT CACCGGCTGC AACACGGGCA TTGGCAAGGA
0181 GACGGTCCTC GAGTTGGCCA AGCGGGGTGC CCGCGT--CT ACATGGCCTG TCGGGATCCC
0241 GGTCGGTGTG AGGCCGCACG ACTGGATATC ATGGATCGCA GTCGCAACCA GCAGCTCTTC
0301 AATCGCACCC TGGATCTGGG CTCCCTGCAG TCGGTGAGAA ATTTCGTGGA GCGCTTCAAG
0361 GCGGAGGAAT CCCGGCTGGA TATACTGATC AATAATGCCG GCGTTATGGC CTGTCCCAGG
0421 ACCCTCACAG CCGATGGCTT TGAGCAGCAG TTCGGTGTCA ATCATCTGGG ACACTTTTTG
0481 CTCACGAATC TGCTGCTGGA TC |
| Assemble Data |
3842R-2.IR_full 1 GACTCTCTGCTTCCGGGGTTTCTGGGCCTGGGCCGTGCTGATATTCCTGATTGTTCTGGG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3842R-2.in silico 1 GACTCTCTGCTTCCGGGGTTTCTGGGCCTGGGCCGTGCTGATATTCCTGATTGTTCTGGG 60
3842R-2.IR_full 61 CATATTGCTCTTCATGTGGCTGCTGCGCAAGTGCATCCAAGGACCGGCCTACCGGAAGGC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3842R-2.in silico 61 CATATTGCTCTTCATGTGGCTGCTGCGCAAGTGCATCCAAGGACCGGCCTACCGGAAGGC 120
3842R-2.IR_full 121 CAATCGCATCGATGGCAAGGTGGTCATCGTCACCGGCTGCAACACGGGCATTGGCAAGGA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3842R-2.in silico 121 CAATCGCATCGATGGCAAGGTGGTCATCGTCACCGGCTGCAACACGGGCATTGGCAAGGA 180
3842R-2.IR_full 181 GACGGTCCTCGAGTTGGCCAAGCGGGGTGCCCGCGTNCCTACATGGCCTGTCGGGATCCC 240
|||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||
3842R-2.in silico 181 GACGGTCCTCGAGTTGGCCAAGCGGGGTGCCCGCGT--CTACATGGCCTGTCGGGATCCC 240
3842R-2.IR_full 241 GGTCGGTGTGAGGCCGCACGACTGGATATCATGGATCGCAGTCGCAACCAGCAGCTCTTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3842R-2.in silico 241 GGTCGGTGTGAGGCCGCACGACTGGATATCATGGATCGCAGTCGCAACCAGCAGCTCTTC 300
3842R-2.IR_full 301 AATCGCACCCTGGATCTGGGCTCCCTGCAGTCGGTGAGAAATTTCGTGGAGCGCTTCAAG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3842R-2.in silico 301 AATCGCACCCTGGATCTGGGCTCCCTGCAGTCGGTGAGAAATTTCGTGGAGCGCTTCAAG 360
3842R-2.IR_full 361 GCGGAGGAATCCCGGCTGGATATACTGATCAATAATGCCGGCGTTATGGCCTGTCCCAGG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3842R-2.in silico 361 GCGGAGGAATCCCGGCTGGATATACTGATCAATAATGCCGGCGTTATGGCCTGTCCCAGG 420
3842R-2.IR_full 421 ACCCTCACAGCCGATGGCTTTGAGCAGCAGTTCGGTGTCAATCATCTGGGACACTTTTTG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3842R-2.in silico 421 ACCCTCACAGCCGATGGCTTTGAGCAGCAGTTCGGTGTCAATCATCTGGGACACTTTTTG 480
3842R-2.IR_full 481 CTCACGAATCTGCTGCTGGATC 502
||||||||||||||||||||||
3842R-2.in silico 481 CTCACGAATCTGCTGCTGGATC 502
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_132088.1 |
CG3842-RA, transcript variant A (CG3842), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_206633.1 |
CG3842-RB, transcript variant B (CG3842), mRNA |
| 2.48 |
12 |
5 |
11 |
38 |
NM_136465.2 |
CG2065-RA (CG2065), mRNA |
| 1.65 |
8 |
4 |
10 |
15 |
NM_136466.2 |
CG2064-RA (CG2064), mRNA |
| 0.2 |
1 |
3 |
2 |
4 |
NM_139891.3 |
CG7462-RC, transcript variant C (Ank2), mRNA |
| 0.2 |
1 |
3 |
2 |
4 |
NM_168227.2 |
CG7462-RB, transcript variant B (Ank2), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_176360.1 |
CG32206-RC, transcript variant C (CG32206), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_168794.2 |
CG32206-RB, transcript variant B (CG32206), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_139990.2 |
CG5971-RA (CG5971), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_168898.1 |
CG32436-RA (CG32436), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_058072.3 |
CG8432-RA (Rep), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_132963.1 |
CG16700-RA (CG16700), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_170116.1 |
CG5422-RB, transcript variant B (Rox8), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_170118.1 |
CG5422-RF, transcript variant F (Rox8), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_170117.1 |
CG5422-RC, transcript variant C (Rox8), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_142361.2 |
CG5319-RA (CG5319), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_166388.1 |
CG11200-RA, transcript variant A (CG11200), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_137627.2 |
CG11200-RB, transcript variant B (CG11200), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_079175.2 |
CG1242-RA (Hsp83), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_079920.2 |
CG6727-RA (gom), mRNA |
| 0 |
0 |
1 |
11 |
15 |
NM_206511.1 |
CG7675-RC, transcript variant C (CG7675), mRNA |
| 0 |
0 |
1 |
11 |
15 |
NM_169809.2 |
CG7675-RA, transcript variant A (CG7675), mRNA |
| 0 |
0 |
1 |
11 |
15 |
NM_142460.2 |
CG7675-RB, transcript variant B (CG7675), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_136338.2 |
CG12792-RA (l(2)09851), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_166616.1 |
CG5411-RA, transcript variant A (Pde8), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_137970.3 |
CG5411-RE, transcript variant E (Pde8), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_080247.2 |
CG13221-RA (Vhl), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_166617.1 |
CG5411-RD, transcript variant D (Pde8), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_166618.1 |
CG5411-RB, transcript variant B (Pde8), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_079356.2 |
CG18492-RA (Tak1), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| +- |
|
| DB |
 |
| +- |
|
| DC |
 |
| ++ |
|
| DM |
 |
| |
|
| VM |
 |
| |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| ++ |
|
| DB |
|
| + |
|
| DC |
|
| ++ |
|
| DM |
|
| + |
|
| VM |
|
| |
|
| VB |
|
| |
|
|
|
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