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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3821R-3 |
| Symbol |
Aats-asp |
Full Name |
Aspartyl-tRNA synthetase |
| CG No |
CG3821 |
Old CG No |
CG3821 |
| Synonyms |
CG3821, aats-asp, poney, l(2)49Db, l(2)k04508, AspRS, DRS, cDNA1, vr1, l(2)vr1, clone 2.49, anon-EST:Liang-2.49, l(2)v27, Aats-asp |
| Accession No (Link to NCBI) |
NM_057261.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Arsham AM, Neufeld TP.
A genetic screen in Drosophila reveals novel cytoprotective functions of the autophagy-lysosome pathway.
PLoS One (2009) 4(6) e6068
[
PubMed ID = 19562034
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGGCCGCTAAGAAAGCCGAAC |
| Primer Seq. 3' |
aatctagaggtaccACGCGATGCATCCTCGATTTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AGGCCGCTAA GAAAGCCGAA CAAAAAGCGG AGAACGCCTC CACCGCGGCG GCCAATAACG
0061 CTGGTGGCGA TTCTGCGGAG GATCACGCCG CCGGAAGATA TGGCCTCTCG GAAATGATCC
0121 AATCCAAGGA CAAACGCAGT GANACGCAAC TTTGTCCCGG TTTCGGAACT AAGCGGTCAG
0181 GTGGGCAAAG GACTGGTCTG GGTGCGAGGA CGCGTCCACA CTTCGAGAGC CAAGGGAAAG
0241 CAGTGCTTCC TCATCCTGCG CCAGCAGAGC AGCACGGTGC AGTGCATCCT GGCTGTCGGT
0301 GATGTTATCT CCAAACAGAT GGTCAAATTT GCGGGAAATA TCCCTAAGGA GAGCATTATT
0361 GATATCCAGG CCAAGCCAGT GGCGGTGTCT AGCAAGATTG AATCCTGCAC AGAGCAGTCG
0421 CTNGGAATTG TCCGTCGAGC AGATATTCGT AATATCCCAG GCCAAGGCAC AGTTGCCATT
0481 GCAAATCGAG GATGCATCNC GT |
| in silico PCR Fragment |
0001 AGGCCGCTAA GAAAGCCGAA CAAAAAGCGG AGAACGCCTC CACCGCGGCG GCCAATAACG
0061 CTGGTGGCGA TTCTGCGGAG GATCACGCCG CCGGAAGATA TGGCCTCTCG GAAATGATCC
0121 AATCCAAGGA CAAACGCAGT GA-ACGCAAC TTTGTCCCGG TTTCGGAACT AAGCGGTCAG
0181 GTGGGCAAAG GACTGGTCTG GGTGCGAGGA CGCGTCCACA CTTCGAGAGC CAAGGGAAAG
0241 CAGTGCTTCC TCATCCTGCG CCAGCAGAGC AGCACGGTGC AGTGCATCCT GGCTGTCGGT
0301 GATGTTATCT CCAAACAGAT GGTCAAATTT GCGGGAAATA TCCCTAAGGA GAGCATTATT
0361 GATATCCAGG CCAAGCCAGT GGCGGTGTCT AGCAAGATTG AATCCTGCAC AGAGCAGTCG
0421 CT-GGAATTG TCCGTCGAGC AGATATTCGT AATATCCCAG GCCAAGGCAC AGTTGCCATT
0481 GCAAATCGAG GATGCATCGC GT |
| Assemble Data |
3821R-3.IR_full 1 AGGCCGCTAAGAAAGCCGAACAAAAAGCGGAGAACGCCTCCACCGCGGCGGCCAATAACG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3821R-3.in silico 1 AGGCCGCTAAGAAAGCCGAACAAAAAGCGGAGAACGCCTCCACCGCGGCGGCCAATAACG 60
3821R-3.IR_full 61 CTGGTGGCGATTCTGCGGAGGATCACGCCGCCGGAAGATATGGCCTCTCGGAAATGATCC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3821R-3.in silico 61 CTGGTGGCGATTCTGCGGAGGATCACGCCGCCGGAAGATATGGCCTCTCGGAAATGATCC 120
3821R-3.IR_full 121 AATCCAAGGACAAACGCAGTGANACGCAACTTTGTCCCGGTTTCGGAACTAAGCGGTCAG 180
|||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
3821R-3.in silico 121 AATCCAAGGACAAACGCAGTGA-ACGCAACTTTGTCCCGGTTTCGGAACTAAGCGGTCAG 180
3821R-3.IR_full 181 GTGGGCAAAGGACTGGTCTGGGTGCGAGGACGCGTCCACACTTCGAGAGCCAAGGGAAAG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3821R-3.in silico 181 GTGGGCAAAGGACTGGTCTGGGTGCGAGGACGCGTCCACACTTCGAGAGCCAAGGGAAAG 240
3821R-3.IR_full 241 CAGTGCTTCCTCATCCTGCGCCAGCAGAGCAGCACGGTGCAGTGCATCCTGGCTGTCGGT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3821R-3.in silico 241 CAGTGCTTCCTCATCCTGCGCCAGCAGAGCAGCACGGTGCAGTGCATCCTGGCTGTCGGT 300
3821R-3.IR_full 301 GATGTTATCTCCAAACAGATGGTCAAATTTGCGGGAAATATCCCTAAGGAGAGCATTATT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3821R-3.in silico 301 GATGTTATCTCCAAACAGATGGTCAAATTTGCGGGAAATATCCCTAAGGAGAGCATTATT 360
3821R-3.IR_full 361 GATATCCAGGCCAAGCCAGTGGCGGTGTCTAGCAAGATTGAATCCTGCACAGAGCAGTCG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3821R-3.in silico 361 GATATCCAGGCCAAGCCAGTGGCGGTGTCTAGCAAGATTGAATCCTGCACAGAGCAGTCG 420
3821R-3.IR_full 421 CTNGGAATTGTCCGTCGAGCAGATATTCGTAATATCCCAGGCCAAGGCACAGTTGCCATT 480
|| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3821R-3.in silico 421 CT-GGAATTGTCCGTCGAGCAGATATTCGTAATATCCCAGGCCAAGGCACAGTTGCCATT 480
3821R-3.IR_full 481 GCAAATCGAGGATGCATCNCGT 502
|||||||||||||||||| |||
3821R-3.in silico 481 GCAAATCGAGGATGCATCGCGT 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_057261.2 |
CG3821-RA (Aats-asp), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_144446.1 |
CG18869-RA (CG18869), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_080004.2 |
CG18869-RA (CG18869), mRNA, ankyrin repeat, tyrosine kinase CG18247-RA (shark), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_079797.2 |
CG6348-RA, transcript variant A (ro), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_170294.1 |
CG6348-RB, transcript variant B (ro), mRNA |
| 0 |
0 |
1 |
5 |
9 |
NM_143496.3 |
CG15523-RA (CG15523), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_166413.2 |
CG10052-RA (Rx), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_137043.2 |
CG13333-RA (CG13333), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_164316.2 |
CG31516-RA (CG31516), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_001014460.2 |
CG7085-RD, transcript variant D (l(2)s5379), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_134814.4 |
CG7085-RA, transcript variant A (l(2)s5379), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_001032049.1 |
CG7085-RE, transcript variant E (l(2)s5379), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_167927.1 |
CG1960-RB, transcript variant B (mu2), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_079163.2 |
CG1960-RA, transcript variant A (mu2), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_141273.2 |
CG16708-RB, transcript variant B (CG16708), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_169050.1 |
CG16708-RA, transcript variant A (CG16708), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_079491.2 |
CG5723-RB (Ten-m), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001043128.1 |
CG7422-RB (CG7422), mRNA |
| 0 |
0 |
0 |
6 |
6 |
NM_143754.2 |
CG18660-RC, transcript variant C (Nckx30C), mRNA |
| 0 |
0 |
0 |
6 |
6 |
NM_164870.1 |
CG18660-RB, transcript variant B (Nckx30C), mRNA |
| 0 |
0 |
0 |
6 |
6 |
NM_164869.1 |
CG18660-RA, transcript variant A (Nckx30C), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_130624.2 |
CG16903-RA (CG16903), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_135247.2 |
CG9138-RA (SP1070), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_139492.2 |
CG2103-RA, transcript variant A (pgant6), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_167966.1 |
CG2103-RB, transcript variant B (pgant6), mRNA |
| 0 |
0 |
0 |
3 |
9 |
NM_139858.2 |
CG8564-RA (CG8564), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_134789.1 |
CG15356-RA (CG15356), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_058000.2 |
CG1982-RA (Sodh-1), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_079587.2 |
CG4649-RA (Sodh-2), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_132978.2 |
CG10598-RA, transcript variant A (CG10598), mRNA |
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