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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 3783R-1 
 Symbol CG32809  Full Name CG32809 
 CG No CG32809  Old CG No CG3783 
 Synonyms EG:171E4.3, CG3791, CG3783, CG3737, EG:9D2.2, EG:73D1.1, CG32809 
 Accession No (Link to NCBI) NM_166899.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCAGCAGCTCCTCGTCGAAGAA 
 Primer Seq. 3' 
aatctagaggtaccTGATCTCGTTGGGCAGCAGGG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CAGCAGCTCC TCGTCGAAGA AGAAGCGCAA GGGTAGAGAG GGCGAAGAAG ACTGGCAGAA 
0061 TGAGGCAAAG TCGGGACGGC TTCCGTCGAA TGAACAGGGT GGCGACGAAA GACGACGAGC 
0121 CATGCGANCG CGACGATCCA CGACGACATA CGCTCGGCGG CGATATGCTG GTCTACGGCT 
0181 CGCAGCATCC ACACGCCCAT CAGATGGCGC CCCAGCAACA GCGGGCCATG GATCTGGAGA 
0241 TGAGTACGCG CGCGCAGAAG AACAAAAAGA ACCAGCCGAT GCGCGGTTAT GCGCCTGTTA 
0301 ACCAGGGACC GCTGTTCGAC GACGATCCCG GCATCATGTC GGAGGTGGAA ACGGCCAGCA 
0361 CCGGATTCCG GAGGGGCGGC ANAGCAgNNC TCCTCGCTCC CCGTGGTACG CACGCCCTCG 
0421 AAAACTCTGG AACGACCGCT GGGCCTGGTA CTTTCTGCAG TACCGCTCGG AGACGAAGCN 
0481 NGcCCTGCTG CCCAANGNGA TCA 
 in silico PCR Fragment 
0001 CAGCAGCTCC TCGTCGAAGA AGAAGCGCAA GGGTAGAGAG GGCGAAGAAG ACTGGCAGAA 
0061 TGAGGCAAAG TCGGGACGGC TTCCGTCGAA TGAACAGGGT GGCGACGAAA GACGACGAGC 
0121 CATGCGA-CG CGACGATCCA CGACGACATA CGCTCGGCGG CGATATGCTG GTCTACGGCT 
0181 CGCAGCATCC ACACGCCCAT CAGATGGCGC CCCAGCAACA GCGGGCCATG GATCTGGAGA 
0241 TGAGTACGCG CGCGCAGAAG AACAAAAAGA ACCAGCCGAT GCGCGGTTAT GCGCCTGTTA 
0301 ACCAGGGACC GCTGTTCGAC GACGATCCCG GCATCATGTC GGAGGTGGAA ACGGCCAGCA 
0361 CCGGATTCCG GAGGGGCGGC A-AGCAGCGC TCCTCGCTCC CCGTGGTACG CACGCCCTCG 
0421 AAAACTCTGG AACGACCGCT GGGCCTGGT- CTTTCTGCAG TACCGCTCGG AGACGAAGCG 
0481 CGCCCTGCTG CCCAACGAGA TCA 
 Assemble Data 

3783R-1.IR_full       1   CAGCAGCTCCTCGTCGAAGAAGAAGCGCAAGGGTAGAGAGGGCGAAGAAGACTGGCAGAA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3783R-1.in silico     1   CAGCAGCTCCTCGTCGAAGAAGAAGCGCAAGGGTAGAGAGGGCGAAGAAGACTGGCAGAA 60


3783R-1.IR_full       61  TGAGGCAAAGTCGGGACGGCTTCCGTCGAATGAACAGGGTGGCGACGAAAGACGACGAGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3783R-1.in silico     61  TGAGGCAAAGTCGGGACGGCTTCCGTCGAATGAACAGGGTGGCGACGAAAGACGACGAGC 120


3783R-1.IR_full       121 CATGCGANCGCGACGATCCACGACGACATACGCTCGGCGGCGATATGCTGGTCTACGGCT 180
                          ||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
3783R-1.in silico     121 CATGCGA-CGCGACGATCCACGACGACATACGCTCGGCGGCGATATGCTGGTCTACGGCT 180


3783R-1.IR_full       181 CGCAGCATCCACACGCCCATCAGATGGCGCCCCAGCAACAGCGGGCCATGGATCTGGAGA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3783R-1.in silico     181 CGCAGCATCCACACGCCCATCAGATGGCGCCCCAGCAACAGCGGGCCATGGATCTGGAGA 240


3783R-1.IR_full       241 TGAGTACGCGCGCGCAGAAGAACAAAAAGAACCAGCCGATGCGCGGTTATGCGCCTGTTA 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3783R-1.in silico     241 TGAGTACGCGCGCGCAGAAGAACAAAAAGAACCAGCCGATGCGCGGTTATGCGCCTGTTA 300


3783R-1.IR_full       301 ACCAGGGACCGCTGTTCGACGACGATCCCGGCATCATGTCGGAGGTGGAAACGGCCAGCA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3783R-1.in silico     301 ACCAGGGACCGCTGTTCGACGACGATCCCGGCATCATGTCGGAGGTGGAAACGGCCAGCA 360


3783R-1.IR_full       361 CCGGATTCCGGAGGGGCGGCANAGCAGNNCTCCTCGCTCCCCGTGGTACGCACGCCCTCG 420
                          ||||||||||||||||||||| |||||  |||||||||||||||||||||||||||||||
3783R-1.in silico     361 CCGGATTCCGGAGGGGCGGCA-AGCAGCGCTCCTCGCTCCCCGTGGTACGCACGCCCTCG 420


3783R-1.IR_full       421 AAAACTCTGGAACGACCGCTGGGCCTGGTACTTTCTGCAGTACCGCTCGGAGACGAAGCN 480
                          ||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||| 
3783R-1.in silico     421 AAAACTCTGGAACGACCGCTGGGCCTGGT-CTTTCTGCAGTACCGCTCGGAGACGAAGCG 480


3783R-1.IR_full        481 GCCCTGCTGCCCAANGNGATCA 502
                           |||||||||||||| | |||||
3783R-1.in silico      481 GCCCTGCTGCCCAACGAGATCA 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_130578.2  CG32809-RD, transcript variant D (CG32809), mRNA 
100   482  0  0  0  NM_166899.1  CG32809-RB, transcript variant B (CG32809), mRNA 
0   0  4  3  4  NM_142386.2  CG7523-RA (CG7523), mRNA 
0   0  3  5  2  NM_057733.3  CG5210-RA (Chit), mRNA 
0   0  2  8  5  NM_079224.3  CG10037-RA (vvl), mRNA 
0   0  2  3  4  NM_079481.2  CG7152-RB, transcript variant B (Syn1), mRNA 
0   0  2  3  4  NM_168937.2  CG7152-RA, transcript variant A (Syn1), mRNA 
0   0  2  3  2  NM_136536.2  CG2110-RA (Cyp4ad1), mRNA 
0   0  2  3  2  NM_176271.1  CG2469-RB, transcript variant B (CG2469), mRNA 
0   0  2  3  2  NM_176270.1  CG2469-RA, transcript variant A (CG2469), mRNA 
0   0  1  6  4  NM_140936.2  CG17147-RA (CG17147), mRNA 
0   0  1  4  6  NM_079711.2  CG6545-RA (lbe), mRNA 
0   0  1  4  4  NM_132092.2  CG3869-RA, transcript variant A (Marf), mRNA 
0   0  1  4  4  NM_206634.1  CG3869-RC, transcript variant C (Marf), mRNA 
0   0  1  4  4  NM_206635.2  CG3869-RB, transcript variant B (Marf), mRNA 
0   0  1  3  6  NM_139495.1  CG9973-RA (CG9973), mRNA 
0   0  1  3  4  NM_078660.2  CG5424-RB, transcript variant B (f), mRNA 
0   0  1  3  4  NM_001042819.1  CG5424-RF, transcript variant F (f), mRNA 
0   0  1  3  4  NM_176745.1  CG5424-RD, transcript variant D (f), mRNA 
0   0  1  3  4  NM_132457.1  CG11122-RA (CG11122), mRNA 
0   0  1  3  4  NM_176746.1  CG5424-RC, transcript variant C (f), mRNA 
0   0  1  3  2  NM_057853.2  CG2189-RA (Dfd), mRNA 
0   0  1  2  4  NM_134518.2  CG11943-RA, transcript variant A (CG11943), mRNA 
0   0  1  2  4  NM_167687.1  CG11943-RB, transcript variant B (CG11943), mRNA 
0   0  1  2  3  NM_057526.3  CG5920-RA (sop), mRNA 
0   0  1  2  2  NM_133039.3  CG7122-RA, transcript variant A (RhoGAP16F), mRNA 
0   0  1  2  2  NM_176755.1  CG7122-RB, transcript variant B (RhoGAP16F), mRNA 
0   0  1  2  2  NM_133004.1  CG8211-RA (CG8211), mRNA 
0   0  1  2  2  NM_165991.1  CG4832-RC, transcript variant C (cnn), mRNA 
0   0  1  2  2  NM_001014523.1  CG4832-RD, transcript variant D (cnn), mRNA 

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