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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 3781R-2 
 Symbol CG3781  Full Name CG3781 
 CG No CG3781  Old CG No CG3781 
 Synonyms CG3781 
 Accession No (Link to NCBI) NM_132075.3 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment balanced with SM6a, Cy 
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGGCAACGGGAATGAAAACGGG 
 Primer Seq. 3' 
aatctagaggtaccTCGAGGAACTGCTGCTCGGTG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GGCAACGGGA ATGAAAACGG GAATGGAAGT GGAAACGGAA ACACCACAAT GATGCCGCAT 
0061 GGAATCTACC ATGAGCGGCA GACACGTCAC CTGTGCGGCC TCCACGCACT GAACAATCTG 
0121 TTCCAGGGGC CCGACATGTT CTCCAAATCG GAACTGGACG ACTACTGCAC CACATTGACC 
0181 CCGCGCAACT GGCTGAATCC GCATCGATCG TGGATCGGTT GGGGCAACTA CGACGTCAAT 
0241 GTGATCATGT ACGCCCTGCA GCAGCGGAAT TGCGAGGCGG TGTGGTTTGA TCGACGGCGG 
0301 GATCCACACT GTCTCAATCT GAGCGTCATT TTCGGCTTCA TTCTCAATGT ACCGGCTCAG 
0361 ATGAGCCTGG GCTACTACAT CCCACTGCCC TTCCACATGC GACACTGGCT GGCACTGCGT 
0421 CGCTTGAACG GCAGCTACTA CAATCTGGAC TCCAAGCTGC GCGAGCCcaA GTGCCTGGGC 
0481 ACCGAGCAGC AGTTCCTCGA  
 in silico PCR Fragment 
0001 GGCAACGGGA ATGAAAACGG GAATGGAAGT GGAAACGGAA ACACCACAAT GATGCCGCAT 
0061 GGAATCTACC ATGAGCGGCA GACACGTCAC CTGTGCGGCC TCCACGCACT GAACAATCTG 
0121 TTCCAGGGGC CCGACATGTT CTCCAAATCG GAACTGGACG ACTACTGCAC CACATTGACC 
0181 CCGCGCAACT GGCTGAATCC GCATCGATCG TGGATCGGTT GGGGCAACTA CGACGTCAAT 
0241 GTGATCATGT ACGCCCTGCA GCAGCGGAAT TGCGAGGCGG TGTGGTTTGA TCGACGGCGG 
0301 GATCCACACT GTCTCAATCT GAGCGTCATT TTCGGCTTCA TTCTCAATGT ACCGGCTCAG 
0361 ATGAGCCTGG GCTACTACAT CCCACTGCCC TTCCACATGC GACACTGGCT GGCACTGCGT 
0421 CGCTTGAACG GCAGCTACTA CAATCTGGAC TCCAAGCTGC GCGAGCCCAA GTGCCTGGGC 
0481 ACCGAGCAGC AGTTCCTCGA  
 Assemble Data 

3781R-2.IR_full       1   GGCAACGGGAATGAAAACGGGAATGGAAGTGGAAACGGAAACACCACAATGATGCCGCAT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3781R-2.in silico     1   GGCAACGGGAATGAAAACGGGAATGGAAGTGGAAACGGAAACACCACAATGATGCCGCAT 60


3781R-2.IR_full       61  GGAATCTACCATGAGCGGCAGACACGTCACCTGTGCGGCCTCCACGCACTGAACAATCTG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3781R-2.in silico     61  GGAATCTACCATGAGCGGCAGACACGTCACCTGTGCGGCCTCCACGCACTGAACAATCTG 120


3781R-2.IR_full       121 TTCCAGGGGCCCGACATGTTCTCCAAATCGGAACTGGACGACTACTGCACCACATTGACC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3781R-2.in silico     121 TTCCAGGGGCCCGACATGTTCTCCAAATCGGAACTGGACGACTACTGCACCACATTGACC 180


3781R-2.IR_full       181 CCGCGCAACTGGCTGAATCCGCATCGATCGTGGATCGGTTGGGGCAACTACGACGTCAAT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3781R-2.in silico     181 CCGCGCAACTGGCTGAATCCGCATCGATCGTGGATCGGTTGGGGCAACTACGACGTCAAT 240


3781R-2.IR_full       241 GTGATCATGTACGCCCTGCAGCAGCGGAATTGCGAGGCGGTGTGGTTTGATCGACGGCGG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3781R-2.in silico     241 GTGATCATGTACGCCCTGCAGCAGCGGAATTGCGAGGCGGTGTGGTTTGATCGACGGCGG 300


3781R-2.IR_full       301 GATCCACACTGTCTCAATCTGAGCGTCATTTTCGGCTTCATTCTCAATGTACCGGCTCAG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3781R-2.in silico     301 GATCCACACTGTCTCAATCTGAGCGTCATTTTCGGCTTCATTCTCAATGTACCGGCTCAG 360


3781R-2.IR_full       361 ATGAGCCTGGGCTACTACATCCCACTGCCCTTCCACATGCGACACTGGCTGGCACTGCGT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3781R-2.in silico     361 ATGAGCCTGGGCTACTACATCCCACTGCCCTTCCACATGCGACACTGGCTGGCACTGCGT 420


3781R-2.IR_full       421 CGCTTGAACGGCAGCTACTACAATCTGGACTCCAAGCTGCGCGAGCCCAAGTGCCTGGGC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3781R-2.in silico     421 CGCTTGAACGGCAGCTACTACAATCTGGACTCCAAGCTGCGCGAGCCCAAGTGCCTGGGC 480


3781R-2.IR_full       481 ACCGAGCAGCAGTTCCTCGA 500
                          ||||||||||||||||||||
3781R-2.in silico     481 ACCGAGCAGCAGTTCCTCGA 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_132075.3  CG3781-RA (CG3781), mRNA 
0.2   1  4  2  6  NM_141738.3  CG6303-RA (Bruce), mRNA 
0   0  4  3  14  NM_001014720.1  CG3620-RD, transcript variant D (norpA), mRNA 
0   0  4  3  14  NM_167008.1  CG3620-RB, transcript variant B (norpA), mRNA 
0   0  4  3  14  NM_001014721.1  CG3620-RC, transcript variant C (norpA), mRNA 
0   0  4  3  14  NM_080330.2  CG3620-RA, transcript variant A (norpA), mRNA 
0   0  4  3  3  NM_168843.1  CG32225-RA (CG32225), mRNA 
0   0  3  3  3  NM_141698.1  CG8534-RA (CG8534), mRNA 
0   0  3  2  2  NM_078544.2  CG1689-RA (lz), mRNA 
0   0  3  2  2  NM_176546.1  CG4620-RB, transcript variant B (unk), mRNA 
0   0  3  2  2  NM_176545.1  CG4620-RA, transcript variant A (unk), mRNA 
0   0  2  3  3  NM_140522.1  CG16979-RA (CG16979), mRNA 
0   0  1  19  25  NM_168497.1  CG32105-RB (CG32105), mRNA 
0   0  1  5  5  NM_167239.2  CG32677-RA (CG32677), mRNA 
0   0  1  5  5  NM_140725.1  CG18265-RA (CG18265), mRNA 
0   0  1  4  6  NM_175978.1  CG14026-RD, transcript variant D (tkv), mRNA 
0   0  1  4  5  NM_079712.2  CG18402-RA (InR), mRNA 
0   0  1  4  5  NM_169701.2  CG31045-RB, transcript variant B (Mhcl), mRNA 
0   0  1  4  5  NM_001043250.1  CG31045-RG, transcript variant G (Mhcl), mRNA 
0   0  1  4  5  NM_169700.2  CG31045-RA, transcript variant A (Mhcl), mRNA 
0   0  1  4  2  NM_132031.1  CG3097-RA (CG3097), mRNA 
0   0  1  3  3  NM_166483.1  CG30398-RA (CG30398), mRNA 
0   0  1  3  2  NM_139819.2  CG8638-RA (CG8638), mRNA 
0   0  1  2  6  NM_167663.1  CG3917-RB, transcript variant B (Grip84), mRNA 
0   0  1  2  6  NM_078685.2  CG3917-RC, transcript variant C (Grip84), mRNA 
0   0  1  2  6  NM_167662.1  CG3917-RA, transcript variant A (Grip84), mRNA 
0   0  1  2  6  NM_135180.1  CG9511-RA (CG9511), mRNA 
0   0  1  2  5  NM_166993.1  CG2913-RC, transcript variant C (yin), mRNA 
0   0  1  2  5  NM_057800.3  CG2913-RB, transcript variant B (yin), mRNA 
0   0  1  2  5  NM_057799.3  CG2913-RA, transcript variant A (yin), mRNA 

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