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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3776R-1 |
| Symbol |
Jhebp29 |
Full Name |
Juvenile hormone esterase binding protein 29 |
| CG No |
CG3776 |
Old CG No |
CG3776 |
| Synonyms |
EP(2)0835, Jhebp29, CG3776, P29, DmP29 |
| Accession No (Link to NCBI) |
NM_138145.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGCAGCACACGCTTATACGC |
| Primer Seq. 3' |
aatctagaggtaccGGTATATGTCGCTCTCCTTGA |
| Predicted Fragment Size |
502 |
| IR fragment full Seq |
0001 ATGCAGCACA CGCTTATACG CTGCTTGGGC ATGGCCCGGA TATCCCTGAT GCGTTTGCAG
0061 CCCAGACCCA CAGTGGCAGC CTCCGGGGGA CAGGAAGCTG GGTCCATCTC GAAGCCAACA
0121 CAACCGGTCA GTCGGTCTTT CGCCTCGCTG CCGCAGGAGC AAGACAAGAA GGAGCAGAAT
0181 GCCAGAGAGA GCCTTAACCG CCTGCCTCGC CTAATGGACT TTCCAGAGAT CGTGTGGNCA
0241 TCGGCGCTTA ACTCGTTAAA AAATTGGATC ACCATACAGT TCATAATTCG ACCCTACTTC
0301 GACAGCGAGT TTCAACTCAA GGACTTCATA TACGGCGCCA AGCAAGCGCT GCAGGTAGTA
0361 TCCTCAAAAC TGATGGGCGG CGACTTAGAC TCCCTGGACA ATCTTGTCTC GCCCGAGGCG
0421 ANCGCAGAGC TTAGACCGGT AATCCAAAAA CTGTCAATGA CGCAACGGAG GCAGCTAGAA
0481 ATCAAGGAGA GCGACATATA CC |
| in silico PCR Fragment |
0001 ATGCAGCACA CGCTTATACG CTGCTTGGGC ATGGCCCGGA TATCCCTGAT GCGTTTGCAG
0061 CCCAGACCCA CAGTGGCAGC CTCCGGGGGA CAGGAAGCTG GGTCCATCTC GAAGCCAACA
0121 CAACCGGTCA GTCGGTCTTT CGCCTCGCTG CCGCAGGAGC AAGACAAGAA GGAGCAGAAT
0181 GCCAGAGAGA GCCTTAACCG CCTGCCTCGC CTAATGGACT TTCCAGAGAT CGTGTGGCCA
0241 TCGGCGCTTA ACTCGTTAAA AAATTGGATC ACCATACAGT TCATAATTCG ACCCTACTTC
0301 GACAGCGAGT TTCAACTCAA GGACTTCATA TACGGCGCCA AGCAAGCGCT GCAGGTAGTA
0361 TCCTCAAAAC TGATGGGCGG CGACTTAGAC TCCCTGGACA ATCTTGTCTC GCCCGAGGCG
0421 ATCGCAGAGC TTAGACCGGT AATCCAAAAA CTGTCAATGA CGCAACGGAG GCAGCTAGAA
0481 ATCAAGGAGA GCGACATATA CC |
| Assemble Data |
3776R-1.IR_full 1 ATGCAGCACACGCTTATACGCTGCTTGGGCATGGCCCGGATATCCCTGATGCGTTTGCAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3776R-1.in silico 1 ATGCAGCACACGCTTATACGCTGCTTGGGCATGGCCCGGATATCCCTGATGCGTTTGCAG 60
3776R-1.IR_full 61 CCCAGACCCACAGTGGCAGCCTCCGGGGGACAGGAAGCTGGGTCCATCTCGAAGCCAACA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3776R-1.in silico 61 CCCAGACCCACAGTGGCAGCCTCCGGGGGACAGGAAGCTGGGTCCATCTCGAAGCCAACA 120
3776R-1.IR_full 121 CAACCGGTCAGTCGGTCTTTCGCCTCGCTGCCGCAGGAGCAAGACAAGAAGGAGCAGAAT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3776R-1.in silico 121 CAACCGGTCAGTCGGTCTTTCGCCTCGCTGCCGCAGGAGCAAGACAAGAAGGAGCAGAAT 180
3776R-1.IR_full 181 GCCAGAGAGAGCCTTAACCGCCTGCCTCGCCTAATGGACTTTCCAGAGATCGTGTGGNCA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
3776R-1.in silico 181 GCCAGAGAGAGCCTTAACCGCCTGCCTCGCCTAATGGACTTTCCAGAGATCGTGTGGCCA 240
3776R-1.IR_full 241 TCGGCGCTTAACTCGTTAAAAAATTGGATCACCATACAGTTCATAATTCGACCCTACTTC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3776R-1.in silico 241 TCGGCGCTTAACTCGTTAAAAAATTGGATCACCATACAGTTCATAATTCGACCCTACTTC 300
3776R-1.IR_full 301 GACAGCGAGTTTCAACTCAAGGACTTCATATACGGCGCCAAGCAAGCGCTGCAGGTAGTA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3776R-1.in silico 301 GACAGCGAGTTTCAACTCAAGGACTTCATATACGGCGCCAAGCAAGCGCTGCAGGTAGTA 360
3776R-1.IR_full 361 TCCTCAAAACTGATGGGCGGCGACTTAGACTCCCTGGACAATCTTGTCTCGCCCGAGGCG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3776R-1.in silico 361 TCCTCAAAACTGATGGGCGGCGACTTAGACTCCCTGGACAATCTTGTCTCGCCCGAGGCG 420
3776R-1.IR_full 421 ANCGCAGAGCTTAGACCGGTAATCCAAAAACTGTCAATGACGCAACGGAGGCAGCTAGAA 480
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3776R-1.in silico 421 ATCGCAGAGCTTAGACCGGTAATCCAAAAACTGTCAATGACGCAACGGAGGCAGCTAGAA 480
3776R-1.IR_full 481 ATCAAGGAGAGCGACATATACC 502
||||||||||||||||||||||
3776R-1.in silico 481 ATCAAGGAGAGCGACATATACC 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
484 |
0 |
0 |
0 |
NM_138145.2 |
CG3776-RA (CG3776), mRNA |
| 0 |
0 |
4 |
4 |
4 |
NM_142028.1 |
CG14375-RA (CG14375), mRNA |
| 0 |
0 |
1 |
4 |
12 |
NM_175960.3 |
CG33196-RB (dp), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_139928.3 |
CG7999-RA (MED24), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_137081.2 |
CG30483-RA (Prosap), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_057276.3 |
CG4178-RA (Lsp1beta), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_141518.1 |
CG7549-RA, transcript variant A (CG7549), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_169212.1 |
CG7549-RB, transcript variant B (CG7549), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_001043221.1 |
CG7549-RC, transcript variant C (CG7549), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_001042786.1 |
CG9935-RC, transcript variant C (CG9935), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_001042785.1 |
CG9935-RB, transcript variant B (CG9935), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_143674.1 |
CG9935-RA, transcript variant A (CG9935), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_079913.2 |
CG8110-RA, transcript variant A (syd), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_168245.2 |
CG8110-RB, transcript variant B (syd), mRNA |
| 0 |
0 |
0 |
6 |
10 |
NM_139428.1 |
CG11815-RA (CG11815), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_079898.2 |
CG11081-RA, transcript variant A (plexA), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_166804.2 |
CG11081-RB, transcript variant B (plexA), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_166806.2 |
CG11081-RD, transcript variant D (plexA), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_166805.2 |
CG11081-RC, transcript variant C (plexA), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_143420.2 |
CG11888-RA (Rpn2), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_138050.2 |
CG3363-RA (CG3363), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_001042855.1 |
CG40305-RA (FucTC), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_079259.2 |
CG4974-RA (dally), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_141925.1 |
CG10035-RA (CG10035), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_140963.1 |
CG5847-RA (CG5847), mRNA |
| 0 |
0 |
0 |
3 |
11 |
NM_140803.1 |
CG14073-RB, transcript variant B (CG14073), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_080047.2 |
CG8491-RA (kto), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_057523.3 |
CG18412-RA (ph-p), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_080055.2 |
CG3895-RA (ph-d), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_167834.1 |
CG17090-RB, transcript variant B (CG17090), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
| N |
 |
| + |
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| DB |
 |
| + |
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| DC |
 |
| + |
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| DM |
 |
| + |
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| VM |
 |
| + |
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| VB |
 |
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