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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 3775R-3 
 Symbol CG3775  Full Name CG3775 
 CG No CG3775  Old CG No CG3775 
 Synonyms BEST:GH06882, CG3775 
 Accession No (Link to NCBI) NM_132603.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees male lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTCATCCACCGATAGCTTCAGT 
 Primer Seq. 3' 
aatctagaggtaccTCCTTAAGGCTCTGGGGCTTT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TCATCCACCG ATAGCTTCAG TGCCAATACC TCCGTCACCA CTTCAACCGC CCAGTTGGGC 
0061 ACATTATACA GATGCTGCTT GTTGAGTGGC CAGTCGATAA GAACCTACAC CGATGCCCTG 
0121 TCACGAAGTG GTGCCAACTT CCCTCTGCTG GCCAACCATA CTGGTGGATC CTTCGATTGG 
0181 GTTCTGGCCA ATGCCGCCCT GCGTACGTAC GGAGCTCAGG GTCTTTGGCG ACTCTTGGTC 
0241 AAGGACAATT GGCAGTCGGC GGATCAGAGG ATCTTCTACA TGCTGCCACC CAAGTTCGAT 
0301 CTACTGGGCC GCGATGATGA ATTGAATGAG TTTCTGTATC AGCGCTACCT GAAGATTTTG 
0361 CTCTTGGAAT TGGGCTTGCG AGTGCGTAGA GCTNNTCTGC TGGCCGAGAA ATTGGTTGCA 
0421 TTCGAGAAGA GTCTGCAGAA GCTATTGCCC AACGATGCGG AACAGACGTT GGTGCTGCGA 
0481 AAGCCCCAGA GCCTTAAGGA  
 in silico PCR Fragment 
0001 TCATCCACCG ATAGCTTCAG TGCCAATACC TCCGTCACCA CTTCAACCGC CCAGTTGGGC 
0061 ACATTATACA GATGCTGCTT GTTGAGTGGC CAGTCGATAA GAACCTACAC CGATGCCCTG 
0121 TCACGAAGTG GTGCCAACTT CCCTCTGCTG GCCAACCATA CTGGTGGATC CTTCGATTGG 
0181 GTTCTGGCCA ATGCCGCCCT GCGTACGTAC GGAGCTCAGG GTCTTTGGCG ACTCTTGGTC 
0241 AAGGACAATT GGCAGTCGGC GGATCAGAGG ATCTTCTACA TGCTGCCACC CAAGTTCGAT 
0301 CTACTGGGCC GCGATGATGA ATTGAATGAG TTTCTGTATC AGCGCTACCT GAAGATTTTG 
0361 CTCTTGGAAT TGGGCTTGCG AGTGCGTAGA GCTGCTCTGC TGGCCGAGAA ATTGGTTGCA 
0421 TTCGAGAAGA GTCTGCAGAA GCTATTGCCC AACGATGCGG AACAGACGTT GGTGCTGCGA 
0481 AAGCCCCAGA GCCTTAAGGA  
 Assemble Data 

3775R-3.IR_full       1   TCATCCACCGATAGCTTCAGTGCCAATACCTCCGTCACCACTTCAACCGCCCAGTTGGGC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3775R-3.in silico     1   TCATCCACCGATAGCTTCAGTGCCAATACCTCCGTCACCACTTCAACCGCCCAGTTGGGC 60


3775R-3.IR_full       61  ACATTATACAGATGCTGCTTGTTGAGTGGCCAGTCGATAAGAACCTACACCGATGCCCTG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3775R-3.in silico     61  ACATTATACAGATGCTGCTTGTTGAGTGGCCAGTCGATAAGAACCTACACCGATGCCCTG 120


3775R-3.IR_full       121 TCACGAAGTGGTGCCAACTTCCCTCTGCTGGCCAACCATACTGGTGGATCCTTCGATTGG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3775R-3.in silico     121 TCACGAAGTGGTGCCAACTTCCCTCTGCTGGCCAACCATACTGGTGGATCCTTCGATTGG 180


3775R-3.IR_full       181 GTTCTGGCCAATGCCGCCCTGCGTACGTACGGAGCTCAGGGTCTTTGGCGACTCTTGGTC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3775R-3.in silico     181 GTTCTGGCCAATGCCGCCCTGCGTACGTACGGAGCTCAGGGTCTTTGGCGACTCTTGGTC 240


3775R-3.IR_full       241 AAGGACAATTGGCAGTCGGCGGATCAGAGGATCTTCTACATGCTGCCACCCAAGTTCGAT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3775R-3.in silico     241 AAGGACAATTGGCAGTCGGCGGATCAGAGGATCTTCTACATGCTGCCACCCAAGTTCGAT 300


3775R-3.IR_full       301 CTACTGGGCCGCGATGATGAATTGAATGAGTTTCTGTATCAGCGCTACCTGAAGATTTTG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3775R-3.in silico     301 CTACTGGGCCGCGATGATGAATTGAATGAGTTTCTGTATCAGCGCTACCTGAAGATTTTG 360


3775R-3.IR_full       361 CTCTTGGAATTGGGCTTGCGAGTGCGTAGAGCTNNTCTGCTGGCCGAGAAATTGGTTGCA 420
                          |||||||||||||||||||||||||||||||||  |||||||||||||||||||||||||
3775R-3.in silico     361 CTCTTGGAATTGGGCTTGCGAGTGCGTAGAGCTGCTCTGCTGGCCGAGAAATTGGTTGCA 420


3775R-3.IR_full       421 TTCGAGAAGAGTCTGCAGAAGCTATTGCCCAACGATGCGGAACAGACGTTGGTGCTGCGA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3775R-3.in silico     421 TTCGAGAAGAGTCTGCAGAAGCTATTGCCCAACGATGCGGAACAGACGTTGGTGCTGCGA 480


3775R-3.IR_full       481 AAGCCCCAGAGCCTTAAGGA 500
                          ||||||||||||||||||||
3775R-3.in silico     481 AAGCCCCAGAGCCTTAAGGA 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_132603.2  CG3775-RA (CG3775), mRNA 
0   0  5  2  6  NM_164582.1  CG3054-RA, transcript variant A (l(2)k05819), mRNA 
0   0  5  2  5  NM_134985.2  CG3054-RB, transcript variant B (l(2)k05819), mRNA 
0   0  3  4  2  NM_079105.2  CG4084-RA (l(2)not), mRNA 
0   0  3  2  3  NM_057585.2  CG8676-RC, transcript variant C (Hr39), mRNA 
0   0  3  2  3  NM_057584.4  CG8676-RA, transcript variant A (Hr39), mRNA 
0   0  3  2  3  NM_165364.1  CG8676-RD, transcript variant D (Hr39), mRNA 
0   0  3  2  3  NM_134296.2  CG8676-RB, transcript variant B (Hr39), mRNA 
0   0  3  2  2  NM_139396.3  CG2069-RA (Oseg4), mRNA 
0   0  2  4  4  NM_165285.1  CG31797-RA (CG31797), mRNA 
0   0  2  4  3  NM_176557.1  CG33106-RB, transcript variant B (mask), mRNA 
0   0  2  4  3  NM_176556.1  CG33106-RA, transcript variant A (mask), mRNA 
0   0  2  3  2  NM_140629.1  CG16807-RA (CG16807), mRNA 
0   0  1  4  6  NM_001042868.1  CG34124-RA (CG34124), mRNA 
0   0  1  3  2  NM_078854.1  CG18096-RA (TepI), mRNA 
0   0  1  3  2  NM_165355.1  CG9256-RA, transcript variant A (Nhe2), mRNA 
0   0  1  3  2  NM_136244.2  CG9256-RB, transcript variant B (Nhe2), mRNA 
0   0  1  2  9  NM_078559.2  CG18085-RA (sev), mRNA 
0   0  1  2  2  NM_001038881.1  CG3257-RB, transcript variant B (CG3257), mRNA 
0   0  1  2  2  NM_138043.3  CG3257-RA, transcript variant A (CG3257), mRNA 
0   0  0  6  4  NM_176776.2  CG14617-RB, transcript variant B (CG14617), mRNA 
0   0  0  6  4  NM_001038768.1  CG14617-RD, transcript variant D (CG14617), mRNA 
0   0  0  6  4  NM_001038770.1  CG14617-RE, transcript variant E (CG14617), mRNA 
0   0  0  6  4  NM_176775.1  CG14617-RA, transcript variant A (CG14617), mRNA 
0   0  0  6  4  NM_001038769.1  CG14617-RC, transcript variant C (CG14617), mRNA 
0   0  0  5  6  NM_133088.1  CG6632-RA (Ing3), mRNA 
0   0  0  5  3  NM_136679.3  CG1665-RA (CG1665), mRNA 
0   0  0  4  7  NM_138760.2  CG5304-RA (l(2)01810), mRNA 
0   0  0  4  5  NM_080083.3  CG7004-RA, transcript variant A (fwd), mRNA 
0   0  0  4  5  NM_079222.2  CG8339-RA (sfl), mRNA 

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