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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 3763R-2 
 Symbol Fbp2  Full Name Fat body protein 2 
 CG No CG3763  Old CG No CG3763 
 Synonyms CG3763, T19, P6, Fbp2 
 Accession No (Link to NCBI) NM_078798.3 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGTTTGACTGGACCGGTAAG 
 Primer Seq. 3' 
aatctagaggtaccTCGCCCATGGAGCGGGTGAAT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ATGTTTGACT GGACCGGTAA GAATGTTGTC TATGTGGGCA GCTTCAGCGG CATTGGATGG 
0061 CAGATGATGA TGCAGCTAAT GCAAAAGGAC ATCAAGATGA TGGGCATTAT GCATCGCATG 
0121 GAGAACGTTG AGATGATGAA GAAGCTGCAG GCCATTAATC CATCCGTGAA AGTGGTCTTC 
0181 ATGCAAATGA ACCTCATGGA AAAGATGTCG ATCGAACAGG CGATGAAGAA AATGGGTCAA 
0241 ATGATGGGAC ACATTGATGT GATGATCAAT GGCGAGGGTG TCCTGCTCGA CAAGGATGTG 
0301 GAGACTACGA TGGGCATGAA TCTGACTGGC ATGATCCAGT CGACGATGAT GGCCATGCCC 
0361 TACATGGACA AGACACAGAT GGGCATGGGT GGCATGGTGG TTAACATGTC CTCTGTCTAT 
0421 GGCCTGGAAC CCGCGCCCGC CTTTTCTGTC TACGCCGCTG CCATGCACGG CATCCTCGGA 
0481 TTCACCCGCT CCATGGGCGA  
 in silico PCR Fragment 
0001 ATGTTTGACT GGACCGGTAA GAATGTTGTC TATGTGGGCA GCTTCAGCGG CATTGGATGG 
0061 CAGATGATGA TGCAGCTGAT GCAGAAGGAC ATCAAGATGA TGGGCATTAT GCATCGCATG 
0121 GAGAACGTTA AGATGATGAA GAAGCTGCAG GCCATTAATC CATCCGTGAA AGTGGTCTTC 
0181 ATGCAAATGA ACCTCATGGA AAAGATGTCG ATCGAACAGG CGATGAAGAA AATGGGTCAA 
0241 ATGATGGGAC ACATTGATGT GATGATCAAT GGCGAGGGTG TCCTGCTCGA CAAGGATGTG 
0301 GAGACTACGA TGGGCATGAA TCTGACTGGC ATGATCCAGT CGACGATGAT GGCCATGCCC 
0361 TACATGGACA AGACACAGAT GGGCATGGGT GGCATGGTGG TTAACATGTC CTCTGTCTAT 
0421 GGCCTGGAAC CCGCGCCCGC CTTTTCTGTC TACGCCGCTG CCATGCACGG CATCCTCGGA 
0481 TTCACCCGCT CCATGGGCGA  
 Assemble Data 

3763R-2.IR_full       1   ATGTTTGACTGGACCGGTAAGAATGTTGTCTATGTGGGCAGCTTCAGCGGCATTGGATGG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3763R-2.in silico     1   ATGTTTGACTGGACCGGTAAGAATGTTGTCTATGTGGGCAGCTTCAGCGGCATTGGATGG 60


3763R-2.IR_full       61  CAGATGATGATGCAGCTAATGCAAAAGGACATCAAGATGATGGGCATTATGCATCGCATG 120
                          ||||||||||||||||| ||||| ||||||||||||||||||||||||||||||||||||
3763R-2.in silico     61  CAGATGATGATGCAGCTGATGCAGAAGGACATCAAGATGATGGGCATTATGCATCGCATG 120


3763R-2.IR_full       121 GAGAACGTTGAGATGATGAAGAAGCTGCAGGCCATTAATCCATCCGTGAAAGTGGTCTTC 180
                          ||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
3763R-2.in silico     121 GAGAACGTTAAGATGATGAAGAAGCTGCAGGCCATTAATCCATCCGTGAAAGTGGTCTTC 180


3763R-2.IR_full       181 ATGCAAATGAACCTCATGGAAAAGATGTCGATCGAACAGGCGATGAAGAAAATGGGTCAA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3763R-2.in silico     181 ATGCAAATGAACCTCATGGAAAAGATGTCGATCGAACAGGCGATGAAGAAAATGGGTCAA 240


3763R-2.IR_full       241 ATGATGGGACACATTGATGTGATGATCAATGGCGAGGGTGTCCTGCTCGACAAGGATGTG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3763R-2.in silico     241 ATGATGGGACACATTGATGTGATGATCAATGGCGAGGGTGTCCTGCTCGACAAGGATGTG 300


3763R-2.IR_full       301 GAGACTACGATGGGCATGAATCTGACTGGCATGATCCAGTCGACGATGATGGCCATGCCC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3763R-2.in silico     301 GAGACTACGATGGGCATGAATCTGACTGGCATGATCCAGTCGACGATGATGGCCATGCCC 360


3763R-2.IR_full       361 TACATGGACAAGACACAGATGGGCATGGGTGGCATGGTGGTTAACATGTCCTCTGTCTAT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3763R-2.in silico     361 TACATGGACAAGACACAGATGGGCATGGGTGGCATGGTGGTTAACATGTCCTCTGTCTAT 420


3763R-2.IR_full       421 GGCCTGGAACCCGCGCCCGCCTTTTCTGTCTACGCCGCTGCCATGCACGGCATCCTCGGA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3763R-2.in silico     421 GGCCTGGAACCCGCGCCCGCCTTTTCTGTCTACGCCGCTGCCATGCACGGCATCCTCGGA 480


3763R-2.IR_full       481 TTCACCCGCTCCATGGGCGA 500
                          ||||||||||||||||||||
3763R-2.in silico     481 TTCACCCGCTCCATGGGCGA 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_078798.3  CG3763-RA (Fbp2), mRNA 
0.2   1  2  2  4  NM_167928.1  CG1140-RB, transcript variant B (CG1140), mRNA 
0.2   1  2  2  4  NM_139426.2  CG1140-RA, transcript variant A (CG1140), mRNA 
0   0  4  6  17  NM_168659.1  CG4899-RB, transcript variant B (Pdh), mRNA 
0   0  4  6  17  NM_079381.2  CG4899-RA, transcript variant A (Pdh), mRNA 
0   0  4  2  3  NM_170430.2  CG31037-RA (ca), mRNA 
0   0  3  5  13  NM_078517.2  CG2175-RA, transcript variant A (dec-1), mRNA 
0   0  3  5  13  NM_167132.1  CG2175-RB, transcript variant B (dec-1), mRNA 
0   0  3  5  13  NM_167133.1  CG2175-RC, transcript variant C (dec-1), mRNA 
0   0  3  3  23  NM_167387.1  CG32611-RB (CG32611), mRNA 
0   0  3  2  8  NM_132068.1  CG15899-RB (Ca-alpha1T), mRNA 
0   0  2  7  3  NM_057345.2  CG10325-RA, transcript variant A (abd-A), mRNA 
0   0  2  4  6  NM_168090.1  CG32242-RA (CG32242), mRNA 
0   0  2  4  2  NM_136633.2  CG2072-RA (TXBP181-like), mRNA 
0   0  2  3  2  NM_078716.2  CG11488-RA (mRpL10), mRNA 
0   0  2  2  6  NM_134315.2  CG5227-RD, transcript variant D (sdk), mRNA 
0   0  2  2  6  NM_057942.3  CG5227-RC, transcript variant C (sdk), mRNA 
0   0  2  2  6  NM_134314.2  CG5227-RB, transcript variant B (sdk), mRNA 
0   0  2  2  6  NM_057941.3  CG5227-RA, transcript variant A (sdk), mRNA 
0   0  2  2  3  NM_134508.2  CG32528-RA (CG32528), mRNA 
0   0  2  2  3  NM_139863.3  CG8560-RA (CG8560), mRNA 
0   0  2  2  3  NM_001038961.1  CG15888-RB (CG15888), mRNA 
0   0  2  2  2  NM_142630.1  CG10881-RA (CG10881), mRNA 
0   0  1  8  10  NM_135990.2  CG6860-RB, transcript variant B (CG6860), mRNA 
0   0  1  4  6  NM_079543.2  CG1257-RA (alpha-Est3), mRNA 
0   0  1  4  5  NM_132890.2  CG3692-RA (CalpC), mRNA 
0   0  1  3  16  NM_001014576.1  CG7391-RD, transcript variant D (Clk), mRNA 
0   0  1  3  16  NM_001014575.1  CG7391-RE, transcript variant E (Clk), mRNA 
0   0  1  3  16  NM_001014574.1  CG7391-RF, transcript variant F (Clk), mRNA 
0   0  1  3  16  NM_079240.2  CG7391-RA, transcript variant A (Clk), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
+- 
DM
 
VM
 
VB
 
Caspase-3 signal
N
+ 
DB
+ 
DC
+ 
DM
+ 
VM
+ 
VB
 
Dcompartment   Penetrance
     

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