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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3710R-1 |
| Symbol |
TfIIS |
Full Name |
RNA polymerase II elongation factor |
| CG No |
CG3710 |
Old CG No |
CG3710 |
| Synonyms |
TFIIS, l(2)35Cf, l(2)35cF, DmS-II, BG:DS00929.12, TFIISA, TFS-II, l35Cf, br52, l(2)br52, RnpSII, DmSII, CG3710, TfIIS |
| Accession No (Link to NCBI) |
NM_057619.3 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTCCGCATCAAATGCCGCGAAA |
| Primer Seq. 3' |
aatctagaggtaccAACTTCCATCGGTTGCCGCAC |
| Predicted Fragment Size |
478 |
| IR fragment full Seq |
0001 tccgCATCAA ATGCCGCGAA ATGCTGGCCA CCGCACTGAA GATTGGTGAA GTGCCCGAGG
0061 GATGCGGTGA GcCGGAGGAA ATGGCCGCCG AACTGGAGGA TGNCCATTTA CTGCCGAGTT
0121 CAATAACACG GATATGAAGT ACAAGAATCG CATTAGGTCA CGCGTGGCCA ATCTGANAGG
0181 ACCCCAAGAA TCCTGGATTG CGCGGCAACT TTATGTGCGG CGCCGTCACT GCCAAGCAGC
0241 TGGCCAAAAT GACGCCGGAG GAGATGGCCA GCGACGAGAT GAAGAAGCTG CGCGAGAAGT
0301 TCGTCAAGGA GGCCATCAAC GATNCCCAGC TGGCCANCGN GCAGGGCACC AAGACCGATC
0361 TCCTCAAGTG CGCCAAGTGC AAGAAGCGCA ACTGCACCTA CAACCAGCTG CAGACTCGTT
0421 CCGCCGNTGA ACCTATGACC ACCTTCGTCA TGTGCAACGA GTGCGGCAAC CGATGGAAGT
0481 T |
| in silico PCR Fragment |
0001 TCCGCATCAA ATGCCGCGAA ATGCTGGCCA CCGCACTGAA GATTGGTGAA GTGCCCGAAG
0061 GATGCGGTGA GCCGGAGGAA ATGGCCGCCG AACTGGAGGA TG-CCATTTA CT-CCGAGTT
0121 CAATAACACG GATATGAAGT ACAAGAATCG CATTAGGTCA CGCGTGGCCA ATCTGA-AGG
0181 ACCCCAAAAA TCCTGGATTG CGAGGCAACT TTATGTGCGG CGCCGTCACT GCCAAGCAGC
0241 TGGCCAAAAT GACGCCGGAG GAGATGGCCA GCGATGAGAT GAAGAAGCTG CGCGAGAAGT
0301 TCGTCAAGGA GGCCATCAAC GATGCCCAGC TGGCCACCGT GCAGGGCACC AAGACCGATC
0361 TCCTCAAGTG CGCCAAGTGC AAGAAGCGCA ACTGCACCTA CAACCAGCTG CAGACTCGTT
0421 CCGCCGATGA ACCTATGACC ACCTTCGTCA TGTGCAACGA GTGCGGCAAC CGATGGAAGT
0481 T |
| Assemble Data |
3710R-1.IR_full 1 TCCGCATCAAATGCCGCGAAATGCTGGCCACCGCACTGAAGATTGGTGAAGTGCCCGAGG 60
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
3710R-1.in silico 1 TCCGCATCAAATGCCGCGAAATGCTGGCCACCGCACTGAAGATTGGTGAAGTGCCCGAAG 60
3710R-1.IR_full 61 GATGCGGTGAGCCGGAGGAAATGGCCGCCGAACTGGAGGATGNCCATTTACTGCCGAGTT 120
|||||||||||||||||||||||||||||||||||||||||| ||||||||| |||||||
3710R-1.in silico 61 GATGCGGTGAGCCGGAGGAAATGGCCGCCGAACTGGAGGATG-CCATTTACT-CCGAGTT 120
3710R-1.IR_full 121 CAATAACACGGATATGAAGTACAAGAATCGCATTAGGTCACGCGTGGCCAATCTGANAGG 180
|||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
3710R-1.in silico 121 CAATAACACGGATATGAAGTACAAGAATCGCATTAGGTCACGCGTGGCCAATCTGA-AGG 180
3710R-1.IR_full 181 ACCCCAAGAATCCTGGATTGCGCGGCAACTTTATGTGCGGCGCCGTCACTGCCAAGCAGC 240
||||||| |||||||||||||| |||||||||||||||||||||||||||||||||||||
3710R-1.in silico 181 ACCCCAAAAATCCTGGATTGCGAGGCAACTTTATGTGCGGCGCCGTCACTGCCAAGCAGC 240
3710R-1.IR_full 241 TGGCCAAAATGACGCCGGAGGAGATGGCCAGCGACGAGATGAAGAAGCTGCGCGAGAAGT 300
|||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
3710R-1.in silico 241 TGGCCAAAATGACGCCGGAGGAGATGGCCAGCGATGAGATGAAGAAGCTGCGCGAGAAGT 300
3710R-1.IR_full 301 TCGTCAAGGAGGCCATCAACGATNCCCAGCTGGCCANCGNGCAGGGCACCAAGACCGATC 360
||||||||||||||||||||||| |||||||||||| || ||||||||||||||||||||
3710R-1.in silico 301 TCGTCAAGGAGGCCATCAACGATGCCCAGCTGGCCACCGTGCAGGGCACCAAGACCGATC 360
3710R-1.IR_full 361 TCCTCAAGTGCGCCAAGTGCAAGAAGCGCAACTGCACCTACAACCAGCTGCAGACTCGTT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3710R-1.in silico 361 TCCTCAAGTGCGCCAAGTGCAAGAAGCGCAACTGCACCTACAACCAGCTGCAGACTCGTT 420
3710R-1.IR_full 421 CCGCCGNTGAACCTATGACCACCTTCGTCATGTGCAACGAGTGCGGCAACCGATGGAAGT 480
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
3710R-1.in silico 421 CCGCCGATGAACCTATGACCACCTTCGTCATGTGCAACGAGTGCGGCAACCGATGGAAGT 480
3710R-1.IR_full 481 T 481
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3710R-1.in silico 481 T 481
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
460 |
0 |
0 |
6 |
NM_057619.3 |
CG3710-RA (TfIIS), mRNA |
| 0.86 |
4 |
3 |
2 |
3 |
NM_141412.1 |
CG15180-RA (CG15180), mRNA |
| 0.43 |
2 |
25 |
29 |
57 |
NM_132821.1 |
CG8117-RA (CG8117), mRNA |
| 0.21 |
1 |
5 |
7 |
6 |
NM_165086.1 |
CG15288-RB, transcript variant B (wb), mRNA |
| 0.21 |
1 |
5 |
7 |
6 |
NM_057442.3 |
CG15288-RA, transcript variant A (wb), mRNA |
| 0.21 |
1 |
3 |
2 |
4 |
NM_168001.1 |
CG32271-RA (CG32271), mRNA |
| 0.21 |
1 |
2 |
2 |
7 |
NM_137373.2 |
CG4827-RA (CG4827), mRNA |
| 0 |
0 |
6 |
4 |
3 |
NM_136785.2 |
CG7712-RA (CG7712), mRNA |
| 0 |
0 |
4 |
6 |
18 |
NM_135156.2 |
CG13991-RA (CG13991), mRNA |
| 0 |
0 |
4 |
3 |
2 |
NM_139508.2 |
CG32486-RD (CG32486), mRNA |
| 0 |
0 |
4 |
2 |
3 |
NM_140595.1 |
CG13054-RA (CG13054), mRNA |
| 0 |
0 |
3 |
5 |
7 |
NM_132905.2 |
CG9902-RA (CG9902), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_134972.1 |
CG10039-RA (CG10039), mRNA |
| 0 |
0 |
2 |
7 |
4 |
NM_080212.1 |
CG5832-RA (Hmx), mRNA |
| 0 |
0 |
2 |
6 |
5 |
NM_134618.2 |
CG14619-RA, transcript variant A (CG14619), mRNA |
| 0 |
0 |
2 |
6 |
5 |
NM_167775.1 |
CG14619-RD, transcript variant D (CG14619), mRNA |
| 0 |
0 |
2 |
6 |
5 |
NM_167776.1 |
CG14619-RE, transcript variant E (CG14619), mRNA |
| 0 |
0 |
2 |
6 |
5 |
NM_167777.1 |
CG14619-RC, transcript variant C (CG14619), mRNA |
| 0 |
0 |
2 |
6 |
3 |
NM_167778.1 |
CG14619-RB, transcript variant B (CG14619), mRNA |
| 0 |
0 |
2 |
6 |
3 |
NM_141619.2 |
CG8444-RA (CG8444), mRNA |
| 0 |
0 |
2 |
5 |
4 |
NM_057324.3 |
CG5109-RA (Pcl), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_166211.2 |
CG30459-RA (CG30459), mRNA |
| 0 |
0 |
2 |
4 |
9 |
NM_142836.2 |
CG6990-RA (HP1c), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_205891.1 |
CG3539-RD, transcript variant D (Slh), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_205892.1 |
CG3539-RC, transcript variant C (Slh), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_136254.2 |
CG31988-RA (CG31988), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_165361.1 |
CG31624-RA (CG31624), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_078590.2 |
CG1771-RB, transcript variant B (mew), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_167354.1 |
CG1771-RA, transcript variant A (mew), mRNA |
| 0 |
0 |
2 |
3 |
14 |
NM_057511.3 |
CG3936-RA (N), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| + |
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| DC |
 |
| + |
|
| DM |
 |
| ++ |
|
| VM |
 |
| + |
|
| VB |
 |
| |
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