NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 3658R-2

Symbol CDC45L Full Name CDC45L

CG No CG3658 Old CG No CG3658

Synonyms Cdc45, CDC45, CG3658, EG:BACR7A4.11, DmCdc45, D, anon-1Ec, CDC45L

Accession No (Link to NCBI) NM_130524.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Kondo S, Perrimon N.
A genome-wide RNAi screen identifies core components of the G₂-M DNA damage checkpoint.
Sci Signal (2011) 4(154) rs1 [ PubMed ID = 21205937 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCAGCTGGTTGGCAAGCGCATC

Primer Seq. 3'

aatctagaggtaccCGCTCCAGGCGACTGAGCTTA

Predicted Fragment Size

IR fragment full Seq

0001 CAGCTGGTTG GCAAGCGCAT CTTGATCGTG GTCAACTACG ACATCGATGC CATCTGCGCC 
0061 AGTCTGCATC CTGCAGGCCT TGTTCAAGTA CGATCACATG CTGTACACCG TGGTGCCCAT 
0121 AATGGGCGTT ACGGGTCTGA AGCGGGCCTA TGGAGAGCAC CAGGGCGACG TTAAGTACGT 
0181 GGTGTTGGTG AACTGCGGCG GCTGTGTAGA TATTGTGGAG CTCCTGCAGC CGTCGGACGA 
0241 CGTGNACCTT CTTCATATGC GATTCACATC GCCCGTTGGA TGTGTGTAAC ATCTACAGCG 
0301 ATCGGCAGGT GTGCATCTTG GGGGATGCCT CCTTGGAGGA GAATATTCCA GCGTTCGAGA 
0361 CAATTTTCTA TGACTCTGAA GGTGAAGACG AGGATGAGGA CGAGTCCTCT GACACGGAAC 
0421 AGCAGCACGA CGACAGTGGT GCCGGGGAAT CGGACCAGGA GGATCAGGCG CCAAGGAGCC 
0481 GTAAGCTCAG TCGCCTGNNG CG

in silico PCR Fragment

0001 CAGCTGGTTG GCAAGCGCAT CTTGATCGTG GTCAACTACG ACATCGATGC CATCTGCGCC 
0061 AGTC-GCATC CTGCAGGCCT TGTTCAAGTA CGATCACATG CTGTACACCG TGGTGCCCAT 
0121 AATGGGCGTT ACGGGTCTGA AGCGGGCCTA TGGAGAGCAC CAGGGCGACG TTAAGTACGT 
0181 GGTGTTGGTG AACTGCGGCG GCTGTGTAGA TATTGTGGAG CTCCTGCAGC CGTCGGACGA 
0241 CGTG-ACCTT CTTCATATGC GATTCACATC GCCCGTTGGA TGTGTGTAAC ATCTACAGCG 
0301 ATCGGCAGGT GTGCATCTTG GGGGATGCCT CCTTGGAGGA GAATATTCCA GCGTTCGAGA 
0361 CAATTTTCTA TGACTCTGAA GGTGAAGACG AGGATGAGGA CGAGTCCTCT GACACGGAAC 
0421 AGCAGCACGA CGACAGTGGT GCCGGGGAAT CGGACCAGGA GGATCAGGCG CCAAGGAGCC 
0481 GTAAGCTCAG TCGCCTGGAG CG

Assemble Data


3658R-2.IR_full       1   CAGCTGGTTGGCAAGCGCATCTTGATCGTGGTCAACTACGACATCGATGCCATCTGCGCC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3658R-2.in silico     1   CAGCTGGTTGGCAAGCGCATCTTGATCGTGGTCAACTACGACATCGATGCCATCTGCGCC 60


3658R-2.IR_full       61  AGTCTGCATCCTGCAGGCCTTGTTCAAGTACGATCACATGCTGTACACCGTGGTGCCCAT 120
                          |||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
3658R-2.in silico     61  AGTC-GCATCCTGCAGGCCTTGTTCAAGTACGATCACATGCTGTACACCGTGGTGCCCAT 120


3658R-2.IR_full       121 AATGGGCGTTACGGGTCTGAAGCGGGCCTATGGAGAGCACCAGGGCGACGTTAAGTACGT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3658R-2.in silico     121 AATGGGCGTTACGGGTCTGAAGCGGGCCTATGGAGAGCACCAGGGCGACGTTAAGTACGT 180


3658R-2.IR_full       181 GGTGTTGGTGAACTGCGGCGGCTGTGTAGATATTGTGGAGCTCCTGCAGCCGTCGGACGA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3658R-2.in silico     181 GGTGTTGGTGAACTGCGGCGGCTGTGTAGATATTGTGGAGCTCCTGCAGCCGTCGGACGA 240


3658R-2.IR_full       241 CGTGNACCTTCTTCATATGCGATTCACATCGCCCGTTGGATGTGTGTAACATCTACAGCG 300
                          |||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
3658R-2.in silico     241 CGTG-ACCTTCTTCATATGCGATTCACATCGCCCGTTGGATGTGTGTAACATCTACAGCG 300


3658R-2.IR_full       301 ATCGGCAGGTGTGCATCTTGGGGGATGCCTCCTTGGAGGAGAATATTCCAGCGTTCGAGA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3658R-2.in silico     301 ATCGGCAGGTGTGCATCTTGGGGGATGCCTCCTTGGAGGAGAATATTCCAGCGTTCGAGA 360


3658R-2.IR_full       361 CAATTTTCTATGACTCTGAAGGTGAAGACGAGGATGAGGACGAGTCCTCTGACACGGAAC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3658R-2.in silico     361 CAATTTTCTATGACTCTGAAGGTGAAGACGAGGATGAGGACGAGTCCTCTGACACGGAAC 420


3658R-2.IR_full       421 AGCAGCACGACGACAGTGGTGCCGGGGAATCGGACCAGGAGGATCAGGCGCCAAGGAGCC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3658R-2.in silico     421 AGCAGCACGACGACAGTGGTGCCGGGGAATCGGACCAGGAGGATCAGGCGCCAAGGAGCC 480


3658R-2.IR_full       481 GTAAGCTCAGTCGCCTGNNGCG 502
                          |||||||||||||||||  |||
3658R-2.in silico     481 GTAAGCTCAGTCGCCTGGAGCG 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	4	NM_130524.2	CG3658-RA (CDC45L), mRNA
0.62	3	2	3	3	NM_165880.1	CG8841-RC, transcript variant C (CG8841), mRNA
0.62	3	2	3	3	NM_136916.2	CG8841-RA, transcript variant A (CG8841), mRNA
0.62	3	2	3	3	NM_165879.1	CG8841-RB, transcript variant B (CG8841), mRNA
0.41	2	2	3	5	NM_057597.2	CG10719-RA, transcript variant A (brat), mRNA
0.41	2	2	3	5	NM_134302.1	CG10719-RB, transcript variant B (brat), mRNA
0.41	2	2	3	5	NM_206004.1	CG10719-RC, transcript variant C (brat), mRNA
0	0	3	5	14	NM_142457.2	CG7669-RA (CG7669), mRNA
0	0	3	4	2	NM_132125.1	CG4536-RA (CG4536), mRNA
0	0	3	3	2	NM_136942.2	CG8545-RA (CG8545), mRNA
0	0	3	2	5	NM_057556.3	CG4088-RA, transcript variant A (lat), mRNA
0	0	3	2	5	NM_165971.1	CG4088-RB, transcript variant B (lat), mRNA
0	0	3	2	3	NM_132317.1	CG12121-RA (CG12121), mRNA
0	0	2	6	5	NM_141731.1	CG3996-RA (CG3996), mRNA
0	0	2	5	10	NM_136611.2	CG8070-RA (Mys45A), mRNA
0	0	2	5	8	NM_167015.2	CG6998-RC, transcript variant C (ctp), mRNA
0	0	2	5	8	NM_080336.3	CG6998-RA, transcript variant A (ctp), mRNA
0	0	2	5	8	NM_167016.1	CG6998-RD, transcript variant D (ctp), mRNA
0	0	2	5	8	NM_167014.1	CG6998-RB, transcript variant B (ctp), mRNA
0	0	2	4	5	NM_168660.1	CG4753-RA, transcript variant A (CG4753), mRNA
0	0	2	4	5	NM_140630.2	CG4753-RB, transcript variant B (CG4753), mRNA
0	0	2	4	4	NM_079100.2	CG17632-RA (bw), mRNA
0	0	2	4	4	NM_144472.2	CG1898-RA (HBS1), mRNA
0	0	2	3	11	NM_166246.1	CG6424-RA, transcript variant A (CG6424), mRNA
0	0	2	3	11	NM_137405.2	CG6424-RB, transcript variant B (CG6424), mRNA
0	0	2	3	3	NM_136972.2	CG12488-RA (CG12488), mRNA
0	0	2	3	2	NM_141785.1	CG4706-RA (CG4706), mRNA
0	0	2	2	15	NM_140532.2	CG7372-RA (CG7372), mRNA
0	0	2	2	8	NM_164647.1	CG7269-RB, transcript variant B (Hel25E), mRNA
0	0	2	2	8	NM_164648.1	CG7269-RC, transcript variant C (Hel25E), mRNA

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