NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 3630R-2

Symbol CG3630 Full Name CG3630

CG No CG3630 Old CG No CG3630

Synonyms EG:152A3.3, 152A3.3, CG3630

Accession No (Link to NCBI) NM_130627.2

Inserted Chr. lll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGACGGACGTATCGCATGAAC

Primer Seq. 3'

aatctagaggtaccCTCGAAGTCTACCAACTTGTG

Predicted Fragment Size

IR fragment full Seq

0001 TGACGGACGT ATCGCATGAA CTGGGCGCCT TGCGCTTCGT GGTGGACTCA CCACTGTCCT 
0061 CCAAGGTGGC CATGTTCAAC AACCAGGCCA CGCAGCACAA GCAGCTCGCA GCTGCTGAAT 
0121 CCATTTTCCC AGGACGGACG CGCCGCCTCG CCGAAACCCA CTTTCTCGAA GGATCAGTAT 
0181 GGAAAACCAC TGGCCGGCAG CCTTACAGAG ATGCGTGGCC AGAAGGCCAA CATCCACGTG 
0241 ATGAAGGAGA TGCTGGAGCT CTGCCAGATC ATCAATTCGG AGGGCTACGA CGTCAAGGAC 
0301 GAGCCCACTA TGCGTGTGAT CCCCTTCGGC GAACTTTTTA ATATCTATAA CTACATATCC 
0361 GACAAGGTGG TGGGCATCCT GCTGCGGGCC CGCAAACACA AGTTGGTAGA CTTCGAG

in silico PCR Fragment

0001 TGACGGACGT ATCGCATGAA CTGGGCGCCT TGCGCTTCGT GGTGGACTCA CCACTGTCCT 
0061 CCAAGGTGGC CATGTTCAAC AACCAGGCCA CGCAGCACAA GCAG-TCGCA GCTGCTGAAT 
0121 CCATTTTCCC AGGACGGACG CGCCGCCTCG CCGAAACCCA CTTTCTCGAA GGATCAGTAT 
0181 GGAAAACCAC TGGCCGGCAG CCTTACAGAG ATGCGTGGCC AGAAGGCCAA CATCCACGTG 
0241 ATGAAGGAGA TGCTGGAGCT CTGCCAGATC ATCAATTCGG AGGGCTACGA CGTCAAGGAC 
0301 GAGCCCACTA TGCGTGTGAT CCCCTTCGGC GAACTTTTTA ATATCTATAA CTACATATCC 
0361 GACAAGGTGG TGGGCATCCT GCTGCGGGCC CGCAAACACA AGTTGGTAGA CTTCGAG

Assemble Data


3630R-2.IR_full       1   TGACGGACGTATCGCATGAACTGGGCGCCTTGCGCTTCGTGGTGGACTCACCACTGTCCT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3630R-2.in silico     1   TGACGGACGTATCGCATGAACTGGGCGCCTTGCGCTTCGTGGTGGACTCACCACTGTCCT 60


3630R-2.IR_full       61  CCAAGGTGGCCATGTTCAACAACCAGGCCACGCAGCACAAGCAGCTCGCAGCTGCTGAAT 120
                          |||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||
3630R-2.in silico     61  CCAAGGTGGCCATGTTCAACAACCAGGCCACGCAGCACAAGCAG-TCGCAGCTGCTGAAT 120


3630R-2.IR_full       121 CCATTTTCCCAGGACGGACGCGCCGCCTCGCCGAAACCCACTTTCTCGAAGGATCAGTAT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3630R-2.in silico     121 CCATTTTCCCAGGACGGACGCGCCGCCTCGCCGAAACCCACTTTCTCGAAGGATCAGTAT 180


3630R-2.IR_full       181 GGAAAACCACTGGCCGGCAGCCTTACAGAGATGCGTGGCCAGAAGGCCAACATCCACGTG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3630R-2.in silico     181 GGAAAACCACTGGCCGGCAGCCTTACAGAGATGCGTGGCCAGAAGGCCAACATCCACGTG 240


3630R-2.IR_full       241 ATGAAGGAGATGCTGGAGCTCTGCCAGATCATCAATTCGGAGGGCTACGACGTCAAGGAC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3630R-2.in silico     241 ATGAAGGAGATGCTGGAGCTCTGCCAGATCATCAATTCGGAGGGCTACGACGTCAAGGAC 300


3630R-2.IR_full       301 GAGCCCACTATGCGTGTGATCCCCTTCGGCGAACTTTTTAATATCTATAACTACATATCC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3630R-2.in silico     301 GAGCCCACTATGCGTGTGATCCCCTTCGGCGAACTTTTTAATATCTATAACTACATATCC 360


3630R-2.IR_full       361 GACAAGGTGGTGGGCATCCTGCTGCGGGCCCGCAAACACAAGTTGGTAGACTTCGAG 417
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3630R-2.in silico     361 GACAAGGTGGTGGGCATCCTGCTGCGGGCCCGCAAACACAAGTTGGTAGACTTCGAG 417

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	398	0	0	0	NM_130627.2	CG3630-RA (CG3630), mRNA
0.25	1	2	2	6	NM_057804.2	CG1451-RA (Apc), mRNA
0	0	4	3	3	NM_137942.1	CG13550-RA (CG13550), mRNA
0	0	3	3	3	NM_142490.2	CG7714-RA (CG7714), mRNA
0	0	3	2	4	NM_139488.2	CG1240-RA (CG1240), mRNA
0	0	3	2	3	NM_168357.2	CG32045-RB, transcript variant B (fry), mRNA
0	0	3	2	3	NM_001043133.1	CG32045-RD, transcript variant D (fry), mRNA
0	0	2	3	3	NM_137117.1	CG10104-RA (CG10104), mRNA
0	0	2	3	2	NM_135922.2	CG3793-RA (CG3793), mRNA
0	0	2	2	6	NM_176500.1	CG33109-RA (CG33109), mRNA
0	0	2	2	3	NM_141506.2	CG11035-RA (CG11035), mRNA
0	0	2	2	2	NM_142459.2	CG7671-RA (CG7671), mRNA
0	0	1	5	3	NM_137843.2	CG4329-RA, transcript variant A (CG4329), mRNA
0	0	1	5	3	NM_166537.1	CG4329-RB, transcript variant B (CG4329), mRNA
0	0	1	5	2	NM_130646.2	CG14045-RA (CG14045), mRNA
0	0	1	4	6	NM_169052.1	CG2666-RB, transcript variant B (kkv), mRNA
0	0	1	4	6	NM_079509.1	CG2666-RA, transcript variant A (kkv), mRNA
0	0	1	4	6	NM_206430.1	CG2666-RC, transcript variant C (kkv), mRNA
0	0	1	4	3	NM_136131.2	CG10189-RA (CG10189), mRNA
0	0	1	3	8	NM_079502.2	CG1059-RA (Karybeta3), mRNA
0	0	1	3	6	NM_136761.2	CG7614-RA (Mat1), mRNA
0	0	1	3	4	NM_130610.1	CG3600-RA (CG3600), mRNA
0	0	1	3	2	NM_141420.2	CG2342-RA (Ccp84Ag), mRNA
0	0	1	2	4	NM_168263.1	CG17352-RA, transcript variant A (CG17352), mRNA
0	0	1	2	4	NM_168264.1	CG17352-RB, transcript variant B (CG17352), mRNA
0	0	1	2	4	NM_139957.2	CG17352-RC, transcript variant C (CG17352), mRNA
0	0	1	2	4	NM_078681.2	CG32540-RA (CCKLR-17D3), mRNA
0	0	1	2	4	NM_001038906.1	CG17352-RD, transcript variant D (CG17352), mRNA
0	0	1	2	2	NM_169054.1	CG12162-RB, transcript variant B (CG12162), mRNA
0	0	1	2	2	NM_167949.1	CG32306-RC, transcript variant C (CG32306), mRNA

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