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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 3599R-3 
 Symbol CG3599  Full Name CG3599 
 CG No CG3599  Old CG No CG3599 
 Synonyms CT12113, CG3599 
 Accession No (Link to NCBI) NM_132070.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTGTCAGTGCTCTTCAGCAGTT 
 Primer Seq. 3' 
aatctagaggtaccTGATGTCGAGACATACTCGTG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TGTCAGTGCT CTTCAGCAGT TCGCATCAGC TTTCCAAGCC GGAAGATCCA ACCTATACGG 
0061 CAGCAGTGGT GGAGCACAGC CAGCCAGTGG GCGATTCTCC GCGTGCACGC ACCACCTCAG 
0121 CTTCGGAATC CTTTCAAAAG ATCATCCGTG AAGTCGGCGA TGTGGACATC ATAGTGTTTC 
0181 CCGAGCACAT ACTCAATAGT CAGGCCACAG CCACTTTTGT GCCCCATGAG TCGCAGAACA 
0241 TAAACGCCCT GCTACCAAAC GGACTATGAG CTCTTCCTCA TCGAGCTATC CTGCTCGGCG 
0301 AGGGCTAACC ACCTGTATGT GGTCATAAAC GTGGTGGAGA AGGAACTGTG TGCCCATGGA 
0361 GCTGGTTCTG ACACCTACAA TCCTTGTCCA TCGTCTGGCG TTCGGTACTT CAACACAAAT 
0421 GTCGTGTTCG ATCGAAGGGG CAGGATTGTC TCGCGTTACA GAAAAACCCA TTTGTGGCGA 
0481 CACGAGTATG TCTCGACATC A 
 in silico PCR Fragment 
0001 TGTCAGTGCT CTTCAGCAGT TCGCATCAGC TTTCCAAGCC GGAAGATCCA ACCTATACGG 
0061 CAGCAGTGGT GGAGCACAGC CAGCCAGTGG GCGATTCTCC GCGTGCACGC ACCACCTCAG 
0121 CTTCGGAATC CTTTCAAAAG ATCATCCGTG AAGTCGGCGA TGTGGACATC ATAGTGTTTC 
0181 CCGAGCACAT ACTCAATAGT CAGGCCACAG CCACTTTTGT GCCCCATGAG TCGCAGAACA 
0241 T-AACGCCCT GCTACCAAAC GGACTATGAG CTCTTCCTCA TCGAGCTATC CTGCTCGGCG 
0301 AGGGCTAACC ACCTGTATGT GGTCATAAAC GTGGTGGAGA AGGAACTGTG TGCCCATGGA 
0361 GCTGGTTCTG ACACCTACAA TCCTTGTCCA TCGTCTGGCG TTCGGTACTT CAACACAAAT 
0421 GTCGTGTTCG ATCGAAGGGG CAGGATTGTC TCGCGTTACA GAAAAACCCA TTTGTGGCGA 
0481 CACGAGTATG TCTCGACATC A 
 Assemble Data 

3599R-3.IR_full       1   TGTCAGTGCTCTTCAGCAGTTCGCATCAGCTTTCCAAGCCGGAAGATCCAACCTATACGG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3599R-3.in silico     1   TGTCAGTGCTCTTCAGCAGTTCGCATCAGCTTTCCAAGCCGGAAGATCCAACCTATACGG 60


3599R-3.IR_full       61  CAGCAGTGGTGGAGCACAGCCAGCCAGTGGGCGATTCTCCGCGTGCACGCACCACCTCAG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3599R-3.in silico     61  CAGCAGTGGTGGAGCACAGCCAGCCAGTGGGCGATTCTCCGCGTGCACGCACCACCTCAG 120


3599R-3.IR_full       121 CTTCGGAATCCTTTCAAAAGATCATCCGTGAAGTCGGCGATGTGGACATCATAGTGTTTC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3599R-3.in silico     121 CTTCGGAATCCTTTCAAAAGATCATCCGTGAAGTCGGCGATGTGGACATCATAGTGTTTC 180


3599R-3.IR_full       181 CCGAGCACATACTCAATAGTCAGGCCACAGCCACTTTTGTGCCCCATGAGTCGCAGAACA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3599R-3.in silico     181 CCGAGCACATACTCAATAGTCAGGCCACAGCCACTTTTGTGCCCCATGAGTCGCAGAACA 240


3599R-3.IR_full       241 TAAACGCCCTGCTACCAAACGGACTATGAGCTCTTCCTCATCGAGCTATCCTGCTCGGCG 300
                          | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3599R-3.in silico     241 T-AACGCCCTGCTACCAAACGGACTATGAGCTCTTCCTCATCGAGCTATCCTGCTCGGCG 300


3599R-3.IR_full       301 AGGGCTAACCACCTGTATGTGGTCATAAACGTGGTGGAGAAGGAACTGTGTGCCCATGGA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3599R-3.in silico     301 AGGGCTAACCACCTGTATGTGGTCATAAACGTGGTGGAGAAGGAACTGTGTGCCCATGGA 360


3599R-3.IR_full       361 GCTGGTTCTGACACCTACAATCCTTGTCCATCGTCTGGCGTTCGGTACTTCAACACAAAT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3599R-3.in silico     361 GCTGGTTCTGACACCTACAATCCTTGTCCATCGTCTGGCGTTCGGTACTTCAACACAAAT 420


3599R-3.IR_full       421 GTCGTGTTCGATCGAAGGGGCAGGATTGTCTCGCGTTACAGAAAAACCCATTTGTGGCGA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3599R-3.in silico     421 GTCGTGTTCGATCGAAGGGGCAGGATTGTCTCGCGTTACAGAAAAACCCATTTGTGGCGA 480


3599R-3.IR_full       481 CACGAGTATGTCTCGACATCA 501
                          |||||||||||||||||||||
3599R-3.in silico     481 CACGAGTATGTCTCGACATCA 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_132070.1  CG3599-RA (CG3599), mRNA 
0   0  3  14  13  NM_132069.2  CG32754-RA (vanin-like), mRNA 
0   0  1  5  3  NM_142857.2  CG17121-RA (CG17121), mRNA 
0   0  1  3  5  NM_079806.2  CG31062-RA (side), mRNA 
0   0  1  3  3  NM_168259.1  CG7986-RB, transcript variant B (Atg18), mRNA 
0   0  1  3  3  NM_139927.2  CG7986-RA, transcript variant A (Atg18), mRNA 
0   0  1  3  3  NM_206301.1  CG7986-RC, transcript variant C (Atg18), mRNA 
0   0  1  3  2  NM_137626.2  CG9036-RA (CG9036), mRNA 
0   0  1  2  3  NM_141948.1  CG5333-RA (trus), mRNA 
0   0  1  2  2  NM_143294.1  CG14260-RA (CG14260), mRNA 
0   0  1  2  2  NM_132530.2  CG1847-RA, transcript variant A (CG1847), mRNA 
0   0  1  2  2  NM_167311.1  CG1847-RB, transcript variant B (CG1847), mRNA 
0   0  0  12  6  NM_167061.1  CG32751-RA (CG32751), mRNA 
0   0  0  5  5  NM_176121.1  CG33183-RC, transcript variant C (Hr46), mRNA 
0   0  0  5  3  NM_167060.1  CG32750-RA (CG32750), mRNA 
0   0  0  4  9  NM_140203.1  CG6163-RA (CG6163), mRNA 
0   0  0  4  5  NM_079908.2  CG5406-RA, transcript variant A (sif), mRNA 
0   0  0  4  5  NM_206278.1  CG5406-RC, transcript variant C (sif), mRNA 
0   0  0  4  5  NM_168126.1  CG5406-RB, transcript variant B (sif), mRNA 
0   0  0  4  5  NM_079916.2  CG18374-RA, transcript variant A (Gyk), mRNA 
0   0  0  4  5  NM_167803.1  CG18374-RB, transcript variant B (Gyk), mRNA 
0   0  0  4  4  NM_143189.1  CG8957-RA (CG8957), mRNA 
0   0  0  4  4  NM_142432.2  CG7985-RA (CG7985), mRNA 
0   0  0  4  4  NM_139775.1  CG13296-RA (CG13296), mRNA 
0   0  0  4  2  NR_002695.1  CR9337, mRNA 
0   0  0  3  8  NM_132074.1  CG3585-RA (CG3585), mRNA 
0   0  0  3  7  NM_057771.2  CG1864-RB, transcript variant B (Hr38), mRNA 
0   0  0  3  6  NM_137625.1  CG16868-RA (CG16868), mRNA 
0   0  0  3  5  NM_137311.2  CG8566-RD (unc-104), mRNA 
0   0  0  3  4  NM_135603.1  CG6737-RA (CG6737), mRNA 

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