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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 3592R-3 
 Symbol CG3592  Full Name CG3592 
 CG No CG3592  Old CG No CG3592 
 Synonyms frag7, CG3592 
 Accession No (Link to NCBI) NM_130683.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGGGAGAACGCAACCACTGCAA 
 Primer Seq. 3' 
aatctagaggtaccTCGGTGGATGACGTGCAGAGC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GGGAGAACGC AACCACTGCA AAATTGGTTT TCTATGAGTT CGCATGGCCA CTTTTGGTAG 
0061 CCATTTTCGA GGTGAACCTC TACAGGATCA TTCTGGAGCT TTTGGCCCAC ATCCTGCTGA 
0121 TCGCAATAAC CGTGGTGATG GTCAAAAAGA CCTCGGGTTT GGATGTCCAC AGCAGCGGCC 
0181 AGCACGCCCT ATACGCCATT TTGGGACTGT TTCTATGCGT GGGTGAATCT CTGTTGGTAT 
0241 GCCACTCCTG GTGGTTAGGA GACTTCATCT CCGAAAACCG GCTCAACCTA CTGCACATGG 
0301 TATTGGGAAT GGTGGGACTT TGGCTGGGAC TGGTGGGGAT ATTCGCGAAG AGCATCTTCA 
0361 AGAGCAAGAT ACATGAGCCG CACTTTAATT CCAAACATGG ATTGTGTGGC CTTTTGGGAT 
0421 TCCTGCTTAT CGCTGGGGCC GTGGCCAGNT TGGTTTCGCC CTGGTGTGCT TCACCCACCT 
0481 NGCTCTGCAC GTCATCCACC GA 
 in silico PCR Fragment 
0001 GGGAGAACGC AACCACTGCA AAATTGGTTT TCTATGAGTT CGCATGGCCA CTTTTGGTAG 
0061 CCGTTTTCGA GGTGAACCTC TACAGGATCA TTCTGGAGCT TTTGGCCCAC ATCCTGCTGA 
0121 TCGTAATAAC CGTGGTGATG GTCAAAAAGA CCTCGGGTTT GGATGACCAC AGCAGCGGCC 
0181 AGCATGCCCT ATACGCCATT TTGGGACTGT TTCTATGCGT GGGTGAATCT CTGTTGGTAT 
0241 GCCACTCCTG GTGGTTAGGA GACTTCATCT CCGAAAACCG GCTCAACCTT CTGCACATGG 
0301 TATTGGGAAT GGTGGGACTT TGGCTGGGAC TGGTGGGGAT ATTCGCGAAG AGTATCTTCA 
0361 AGAGCAAGAT ACATGAGCCG CACTTTAATT CCAAACATGG ATTGTGTGGC CTTTTGGGAT 
0421 TCCTGCTTAT CGCTGGGGCC GTGGCCAG-- TGGTTTCGCC CTGGTGTGCT TCACCCACCT 
0481 GGCTCTGCAC GTCATCCACC GA 
 Assemble Data 

3592R-3.IR_full       1   GGGAGAACGCAACCACTGCAAAATTGGTTTTCTATGAGTTCGCATGGCCACTTTTGGTAG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3592R-3.in silico     1   GGGAGAACGCAACCACTGCAAAATTGGTTTTCTATGAGTTCGCATGGCCACTTTTGGTAG 60


3592R-3.IR_full       61  CCATTTTCGAGGTGAACCTCTACAGGATCATTCTGGAGCTTTTGGCCCACATCCTGCTGA 120
                          || |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3592R-3.in silico     61  CCGTTTTCGAGGTGAACCTCTACAGGATCATTCTGGAGCTTTTGGCCCACATCCTGCTGA 120


3592R-3.IR_full       121 TCGCAATAACCGTGGTGATGGTCAAAAAGACCTCGGGTTTGGATGTCCACAGCAGCGGCC 180
                          ||| ||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
3592R-3.in silico     121 TCGTAATAACCGTGGTGATGGTCAAAAAGACCTCGGGTTTGGATGACCACAGCAGCGGCC 180


3592R-3.IR_full       181 AGCACGCCCTATACGCCATTTTGGGACTGTTTCTATGCGTGGGTGAATCTCTGTTGGTAT 240
                          |||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
3592R-3.in silico     181 AGCATGCCCTATACGCCATTTTGGGACTGTTTCTATGCGTGGGTGAATCTCTGTTGGTAT 240


3592R-3.IR_full       241 GCCACTCCTGGTGGTTAGGAGACTTCATCTCCGAAAACCGGCTCAACCTACTGCACATGG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||
3592R-3.in silico     241 GCCACTCCTGGTGGTTAGGAGACTTCATCTCCGAAAACCGGCTCAACCTTCTGCACATGG 300


3592R-3.IR_full       301 TATTGGGAATGGTGGGACTTTGGCTGGGACTGGTGGGGATATTCGCGAAGAGCATCTTCA 360
                          |||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
3592R-3.in silico     301 TATTGGGAATGGTGGGACTTTGGCTGGGACTGGTGGGGATATTCGCGAAGAGTATCTTCA 360


3592R-3.IR_full       361 AGAGCAAGATACATGAGCCGCACTTTAATTCCAAACATGGATTGTGTGGCCTTTTGGGAT 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3592R-3.in silico     361 AGAGCAAGATACATGAGCCGCACTTTAATTCCAAACATGGATTGTGTGGCCTTTTGGGAT 420


3592R-3.IR_full       421 TCCTGCTTATCGCTGGGGCCGTGGCCAGNTTGGTTTCGCCCTGGTGTGCTTCACCCACCT 480
                          ||||||||||||||||||||||||||||  ||||||||||||||||||||||||||||||
3592R-3.in silico     421 TCCTGCTTATCGCTGGGGCCGTGGCCAG--TGGTTTCGCCCTGGTGTGCTTCACCCACCT 480


3592R-3.IR_full        481 GCTCTGCACGTCATCCACCGA 501
                           |||||||||||||||||||||
3592R-3.in silico      481 GCTCTGCACGTCATCCACCGA 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_130683.1  CG3592-RA (CG3592), mRNA 
0   0  2  2  6  NM_164966.2  CG6464-RA (salm), mRNA 
0   0  2  2  4  NM_142097.2  CG3307-RA, transcript variant A (pr-set7), mRNA 
0   0  2  2  4  NM_169577.1  CG3307-RB, transcript variant B (pr-set7), mRNA 
0   0  2  2  4  NM_169578.1  CG3307-RC, transcript variant C (pr-set7), mRNA 
0   0  1  4  4  NM_057432.2  CG2762-RA (ush), mRNA 
0   0  1  3  2  NM_058029.3  CG4882-RA (CG4882), mRNA 
0   0  1  2  4  NM_134831.1  CG3609-RA (CG3609), mRNA 
0   0  1  2  3  NM_168455.2  CG7573-RB, transcript variant B (CG7573), mRNA 
0   0  1  2  3  NM_140201.2  CG7573-RA, transcript variant A (CG7573), mRNA 
0   0  1  2  2  NM_141226.2  CG1057-RA, transcript variant A (MED31), mRNA 
0   0  0  6  7  NM_080306.2  CG3443-RB (pcx), mRNA 
0   0  0  5  4  NM_165155.1  CG31737-RA (CG31737), mRNA 
0   0  0  5  3  NM_136047.2  CG10391-RA (Cyp310a1), mRNA 
0   0  0  4  6  NM_001043121.1  CG10579-RJ, transcript variant J (Eip63E), mRNA 
0   0  0  4  6  NM_168036.1  CG10579-RC, transcript variant C (Eip63E), mRNA 
0   0  0  4  6  NM_001043118.1  CG10579-RK, transcript variant K (Eip63E), mRNA 
0   0  0  4  6  NM_168035.1  CG10579-RB, transcript variant B (Eip63E), mRNA 
0   0  0  4  6  NM_001043117.1  CG10579-RH, transcript variant H (Eip63E), mRNA 
0   0  0  4  6  NM_001043120.1  CG10579-RG, transcript variant G (Eip63E), mRNA 
0   0  0  4  6  NM_168034.1  CG10579-RA, transcript variant A (Eip63E), mRNA 
0   0  0  4  6  NM_001043119.1  CG10579-RI, transcript variant I (Eip63E), mRNA 
0   0  0  4  6  NM_001043116.1  CG10579-RF, transcript variant F (Eip63E), mRNA 
0   0  0  4  6  NM_168033.1  CG10579-RE, transcript variant E (Eip63E), mRNA 
0   0  0  4  6  NM_079180.2  CG10579-RD, transcript variant D (Eip63E), mRNA 
0   0  0  4  4  NM_136193.1  CG2493-RA (CG2493), mRNA 
0   0  0  4  4  NM_134606.2  CG1486-RA, transcript variant A (CG1486), mRNA 
0   0  0  4  4  NM_167743.1  CG1486-RB, transcript variant B (CG1486), mRNA 
0   0  0  4  2  NM_132365.1  CG15250-RA (CG15250), mRNA 
0   0  0  4  2  NM_136828.1  CG13209-RA (sha), mRNA 

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