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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3428R-1 |
| Symbol |
pall |
Full Name |
pallbearer |
| CG No |
CG3428 |
Old CG No |
CG3428 |
| Synonyms |
pall, CG3428 |
| Accession No (Link to NCBI) |
NM_140068.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Chen ZS, Wong AKY, Cheng TC, Koon AC, Chan HYE.
FipoQ/FBXO33, a Cullin-1-based ubiquitin ligase complex component modulates ubiquitination and solubility of polyglutamine disease protein.
J Neurochem (2019) 149(6) 781-798
[
PubMed ID = 30685895
]
[
RRC reference
]
Dui W, Lu W, Ma J, Jiao R.
A systematic phenotypic screen of F-box genes through a tissue-specific RNAi-based approach in Drosophila.
J Genet Genomics (2012) 39(8) 397-413
[
PubMed ID = 22884096
]
[
RRC reference
]
Du J, Zhang J, Su Y, Liu M, Ospina JK, Yang S, Zhu AJ.
In vivo RNAi screen reveals neddylation genes as novel regulators of Hedgehog signaling.
PLoS One (2011) 6(9) e24168
[
PubMed ID = 21931660
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCAGGACATTTTGGAGTTGCTC |
| Primer Seq. 3' |
aatctagaggtaccAACCACAAGTCGAGCTCCGGC |
| Predicted Fragment Size |
501 |
| IR fragment full Seq |
0001 CAgGACATTT TGGAGTTGCT CCCCTACGAC GAGGTGGCAA AGAAAACGAG AGGTGTGCAC
0061 TCTATTTAAT TATCTTGGGC AGCAAATATT AAACAAGGGT TTTAACAAGA TCATTATGGC
0121 CCATGCCAAG AACTTCAAGC GCATCAAATC CATGCTGCCA CGACGGGAAT CGGAACGCAG
0181 GAACCACATT CTATCCCGCC ACTCGGACAT ACTTACCTCG ATCGAAACAC GGATCTCGAT
0241 GCTGACTATG ACGTACTCCA AGTTTATTGA CCTCAACATC TGCTGTTTCA TACCTGGTCG
0301 CGTTCTGGAC GAGATCAACA GCATCCTCCG CATTTTGAGC ACCTCCACCA AACAGCTGCG
0361 ACCACATGAA GTGCTGCAGG AGCTGCGCGA CATCTCCTCC ATGGCCATTG AGCATTTCGA
0421 CGAAAAGATT GCTGTGAATT TCAAATTGGA ACACCGAAAA CAATTGGCGG AATCCACGGC
0481 gGCCGGAGCT CGACTTGTGG TT |
| in silico PCR Fragment |
0001 CAGGACATTT TGGAGTTGCT CCCCTACGAC GAGGTGGCAA A-AAAACGAG AGGTGTGCAC
0061 TCTATTTAAT TATCTTGGGC AGCAAATATT AAACAAGGGT TTTAACAAGA TCATTATGGC
0121 CCATGCCAAG AACTTCAAGC GCATCAAATC CATGCTGCCA CGACGGGAAT CGGAACGCAG
0181 GAACCACATT CTATCCCGCC ACTCGGACAT ACTTACCTCG ATCGAAACAC GGATCTCGAT
0241 GCTGACCATG ACGTACTCCA AGTTTATTGA CCTCAACATC TGCTGTTTCA TACCTGGTCG
0301 CGTTCTGGAC GAGATCAACA GCATCCTGCG CATTTTGAGC ACCTCCACTA AACAGCTGCG
0361 ACCACATGAA GTGCTGCAGG AGCTGCGCGA CATCTCCTCC ATGGCCATTG AGCATTTCGA
0421 CGAAAAGATT GCTGTGAATT TCAAATTGGA ACACCGAAAA CAATTGGCGG AATCCACGGC
0481 GGCCGGAGCT CGACTTGTGG TT |
| Assemble Data |
3428R-1.IR_full 1 CAGGACATTTTGGAGTTGCTCCCCTACGACGAGGTGGCAAAGAAAACGAGAGGTGTGCAC 60
||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
3428R-1.in silico 1 CAGGACATTTTGGAGTTGCTCCCCTACGACGAGGTGGCAAA-AAAACGAGAGGTGTGCAC 60
3428R-1.IR_full 61 TCTATTTAATTATCTTGGGCAGCAAATATTAAACAAGGGTTTTAACAAGATCATTATGGC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3428R-1.in silico 61 TCTATTTAATTATCTTGGGCAGCAAATATTAAACAAGGGTTTTAACAAGATCATTATGGC 120
3428R-1.IR_full 121 CCATGCCAAGAACTTCAAGCGCATCAAATCCATGCTGCCACGACGGGAATCGGAACGCAG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3428R-1.in silico 121 CCATGCCAAGAACTTCAAGCGCATCAAATCCATGCTGCCACGACGGGAATCGGAACGCAG 180
3428R-1.IR_full 181 GAACCACATTCTATCCCGCCACTCGGACATACTTACCTCGATCGAAACACGGATCTCGAT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3428R-1.in silico 181 GAACCACATTCTATCCCGCCACTCGGACATACTTACCTCGATCGAAACACGGATCTCGAT 240
3428R-1.IR_full 241 GCTGACTATGACGTACTCCAAGTTTATTGACCTCAACATCTGCTGTTTCATACCTGGTCG 300
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
3428R-1.in silico 241 GCTGACCATGACGTACTCCAAGTTTATTGACCTCAACATCTGCTGTTTCATACCTGGTCG 300
3428R-1.IR_full 301 CGTTCTGGACGAGATCAACAGCATCCTCCGCATTTTGAGCACCTCCACCAAACAGCTGCG 360
||||||||||||||||||||||||||| |||||||||||||||||||| |||||||||||
3428R-1.in silico 301 CGTTCTGGACGAGATCAACAGCATCCTGCGCATTTTGAGCACCTCCACTAAACAGCTGCG 360
3428R-1.IR_full 361 ACCACATGAAGTGCTGCAGGAGCTGCGCGACATCTCCTCCATGGCCATTGAGCATTTCGA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3428R-1.in silico 361 ACCACATGAAGTGCTGCAGGAGCTGCGCGACATCTCCTCCATGGCCATTGAGCATTTCGA 420
3428R-1.IR_full 421 CGAAAAGATTGCTGTGAATTTCAAATTGGAACACCGAAAACAATTGGCGGAATCCACGGC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3428R-1.in silico 421 CGAAAAGATTGCTGTGAATTTCAAATTGGAACACCGAAAACAATTGGCGGAATCCACGGC 480
3428R-1.IR_full 481 GGCCGGAGCTCGACTTGTGGTT 502
||||||||||||||||||||||
3428R-1.in silico 481 GGCCGGAGCTCGACTTGTGGTT 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
483 |
0 |
0 |
0 |
NM_140068.2 |
CG3428-RA (CG3428), mRNA |
| 0 |
0 |
6 |
2 |
9 |
NM_169796.1 |
CG31196-RA, transcript variant A (14-3-3epsilon), mRNA |
| 0 |
0 |
6 |
2 |
9 |
NM_169797.1 |
CG31196-RB, transcript variant B (14-3-3epsilon), mRNA |
| 0 |
0 |
6 |
2 |
9 |
NM_169798.1 |
CG31196-RD, transcript variant D (14-3-3epsilon), mRNA |
| 0 |
0 |
6 |
2 |
9 |
NM_169799.1 |
CG31196-RC, transcript variant C (14-3-3epsilon), mRNA |
| 0 |
0 |
5 |
3 |
2 |
NM_139961.1 |
CG7120-RA (CG7120), mRNA |
| 0 |
0 |
4 |
3 |
3 |
NM_134495.2 |
CG12234-RA (Ranbp21), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_132912.1 |
CG4521-RA (mthl1), mRNA |
| 0 |
0 |
2 |
6 |
2 |
NM_134577.1 |
CG1529-RA (CG1529), mRNA |
| 0 |
0 |
2 |
4 |
7 |
NM_170435.1 |
CG31009-RA (Cad99C), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_169127.1 |
CG31559-RA (CG31559), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_133088.1 |
CG6632-RA (Ing3), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_164902.1 |
CG31717-RA (CG31717), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_167685.1 |
CG12701-RB, transcript variant B (CG12701), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_134512.4 |
CG12701-RA, transcript variant A (CG12701), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_166341.1 |
CG30127-RA (CG30127), mRNA |
| 0 |
0 |
1 |
7 |
5 |
NM_001043140.1 |
CG33260-RB (CG33260), mRNA |
| 0 |
0 |
1 |
7 |
5 |
NM_206360.1 |
CG33261-RF, transcript variant F (Trl), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_142691.1 |
CG5798-RA (CG5798), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_057268.3 |
CG6944-RA (Lam), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_165814.2 |
CG9062-RB (CG9062), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_137177.2 |
CG11808-RA (CG11808), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_135079.4 |
CG14021-RB, transcript variant B (CG14021), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_164640.1 |
CG14021-RA, transcript variant A (CG14021), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_080250.2 |
CG6224-RA (dbo), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_164843.1 |
CG9556-RA, transcript variant A (alien), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_078793.4 |
CG9556-RB, transcript variant B (alien), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_169932.1 |
CG31223-RA (CG31223), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_136471.2 |
CG1406-RA (U2A), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_141883.1 |
CG3397-RA (CG3397), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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