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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3340R-1 |
| Symbol |
Kr |
Full Name |
Kruppel |
| CG No |
CG3340 |
Old CG No |
CG3340 |
| Synonyms |
kr, CG3340, If, Kr, Kruppel, Dm-Kr |
| Accession No (Link to NCBI) |
NM_079143.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Perkins AD, Lee MJ, Tanentzapf G.
The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.
Sci Data (2014) 1 140002
[
PubMed ID = 25977760
]
[
RRC reference
]
Bauke AC, Sasse S, Matzat T, Klämbt C.
A transcriptional network controlling glial development in the Drosophila visual system.
Development (2015) 142(12) 2184-93
[
PubMed ID = 26015542
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTTCCATGTCGCTATCGCCCCC |
| Primer Seq. 3' |
aatctagaggtaccTAGGAGAGCTGCCACTGGAAG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TTCCATGTCG CTATCGCCCC CCATGTCGGC CAACACATCA GCTACAAGCG CCGCTGCGAT
0061 TTATCCAGCT ATGGGTCTCC AACAGGCGGC CGCTGCCTCA GCTTTTGGAA TGCTCTCACC
0121 TACCCAACTT CTGGCTGCAA ACCGACAAGC TGCCGCATTC ATGGCCCAAC TGCCCATGAG
0181 CACATTGGCC AACACTCTCT TTCCACACAA TCCGGCGGCT TTGTTTGGGG CTTGGGCNTG
0241 CCCAACANGT CGCTCCCGCC CCAGGGTACG CATTTACATT CGCCGCCAGC CAGCCCGCAC
0301 TCGCCGCTGT CCACTCCTTT AGGCAGTGGC AAGCACCCAT TAAATTCCCC CAACAGCACT
0361 CCCCAGCACC ATGAGCCAGC GAAGAAGGCT CGAAAGTTAT CGGTTAAGAA GGAGTTTCAG
0421 ACCGAGATCA GCATGAGTGT AAACGATATG TACCATACAT CGGGAGGCCC AATATCTCCG
0481 CCTTCCAGTG GCAGCTCTCC TA |
| in silico PCR Fragment |
0001 TTCCATGTCG CTATCGCCCC CCATGTCGGC CAACACATCA GCTACAAGCG CCGCTGCGAT
0061 TTATCCAGCT ATGGGTCTCC AACAGGCGGC CGCTGCCTCA GCTTTTGGAA TGCTCTCACC
0121 TACCCAACTT CTGGCTGCAA ACCGTCAAGC TGCCGCATTC ATGGCCCAAC TGCCCATGAG
0181 CACATTGGCC AACACTCTCT TTCCACACAA TCCGGCGGCT TTGTTTGGGG CTTGGGC-TG
0241 CCCAACA-GT CGCTCCCGCC CCAGGGTACG CATTTACATT CGCCGCCAGC CAGCCCGCAC
0301 TCGCCGCTGT CCACTCCTTT AGGTAGTGGC AAGCACCCAT TAAATTCCCC CAACAGCACT
0361 CCCCAGCACC ATGAGCCAGC GAAGAAGGCT CGAAAGTTAT CGGTTAAGAA GGAGTTTCAG
0421 ACCGAGATCA GCATGAGTGT AAACGATATG TACCATACAT CGGGAGGCCC AATATCTCCG
0481 CCTTCCAGTG GCAGCTCTCC TA |
| Assemble Data |
3340R-1.IR_full 1 TTCCATGTCGCTATCGCCCCCCATGTCGGCCAACACATCAGCTACAAGCGCCGCTGCGAT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3340R-1.in silico 1 TTCCATGTCGCTATCGCCCCCCATGTCGGCCAACACATCAGCTACAAGCGCCGCTGCGAT 60
3340R-1.IR_full 61 TTATCCAGCTATGGGTCTCCAACAGGCGGCCGCTGCCTCAGCTTTTGGAATGCTCTCACC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3340R-1.in silico 61 TTATCCAGCTATGGGTCTCCAACAGGCGGCCGCTGCCTCAGCTTTTGGAATGCTCTCACC 120
3340R-1.IR_full 121 TACCCAACTTCTGGCTGCAAACCGACAAGCTGCCGCATTCATGGCCCAACTGCCCATGAG 180
|||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
3340R-1.in silico 121 TACCCAACTTCTGGCTGCAAACCGTCAAGCTGCCGCATTCATGGCCCAACTGCCCATGAG 180
3340R-1.IR_full 181 CACATTGGCCAACACTCTCTTTCCACACAATCCGGCGGCTTTGTTTGGGGCTTGGGCNTG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
3340R-1.in silico 181 CACATTGGCCAACACTCTCTTTCCACACAATCCGGCGGCTTTGTTTGGGGCTTGGGC-TG 240
3340R-1.IR_full 241 CCCAACANGTCGCTCCCGCCCCAGGGTACGCATTTACATTCGCCGCCAGCCAGCCCGCAC 300
||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
3340R-1.in silico 241 CCCAACA-GTCGCTCCCGCCCCAGGGTACGCATTTACATTCGCCGCCAGCCAGCCCGCAC 300
3340R-1.IR_full 301 TCGCCGCTGTCCACTCCTTTAGGCAGTGGCAAGCACCCATTAAATTCCCCCAACAGCACT 360
||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
3340R-1.in silico 301 TCGCCGCTGTCCACTCCTTTAGGTAGTGGCAAGCACCCATTAAATTCCCCCAACAGCACT 360
3340R-1.IR_full 361 CCCCAGCACCATGAGCCAGCGAAGAAGGCTCGAAAGTTATCGGTTAAGAAGGAGTTTCAG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3340R-1.in silico 361 CCCCAGCACCATGAGCCAGCGAAGAAGGCTCGAAAGTTATCGGTTAAGAAGGAGTTTCAG 420
3340R-1.IR_full 421 ACCGAGATCAGCATGAGTGTAAACGATATGTACCATACATCGGGAGGCCCAATATCTCCG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3340R-1.in silico 421 ACCGAGATCAGCATGAGTGTAAACGATATGTACCATACATCGGGAGGCCCAATATCTCCG 480
3340R-1.IR_full 481 CCTTCCAGTGGCAGCTCTCCTA 502
||||||||||||||||||||||
3340R-1.in silico 481 CCTTCCAGTGGCAGCTCTCCTA 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079143.2 |
CG3340-RA (Kr), mRNA |
| 0.2 |
1 |
4 |
3 |
3 |
NM_140383.1 |
CG10191-RA (CG10191), mRNA |
| 0 |
0 |
5 |
2 |
2 |
NM_132962.1 |
CG8945-RC (CG8945), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_135605.2 |
CG6729-RA (CG6729), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_135753.2 |
CG9934-RA (CG9934), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_132122.2 |
CG3126-RA, transcript variant A (C3G), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_176694.1 |
CG3126-RB, transcript variant B (C3G), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_135336.2 |
CG7466-RA (CG7466), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_145192.2 |
CG15107-RA (CG15107), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001014500.1 |
CG33558-RA (CG33558), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001032002.1 |
CG33676-RA, transcript variant A (Skeletor), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001032000.1 |
CG14681-RB, transcript variant B (CG14681), mRNA |
| 0 |
0 |
0 |
9 |
16 |
NM_143409.1 |
CG11873-RA (CG11873), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_135169.2 |
CG9493-RA (Pez), mRNA |
| 0 |
0 |
0 |
4 |
10 |
NM_166125.2 |
CG18255-RA, transcript variant A (Strn-Mlck), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_079548.4 |
CG11094-RC, transcript variant C (dsx), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_169202.1 |
CG11094-RA, transcript variant A (dsx), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_169203.1 |
CG11094-RB, transcript variant B (dsx), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_167647.2 |
CG12199-RB, transcript variant B (kek5), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_133154.2 |
CG12199-RA, transcript variant A (kek5), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_166129.2 |
CG18255-RD, transcript variant D (Strn-Mlck), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_133031.1 |
CG6847-RA (CG6847), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_167145.1 |
CG2257-RA, transcript variant A (Ubc-E2H), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_132210.2 |
CG2257-RB, transcript variant B (Ubc-E2H), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_137399.2 |
CG6459-RA (CG6459), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_079138.1 |
CG2692-RA (gsb-n), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_167146.1 |
CG2257-RC, transcript variant C (Ubc-E2H), mRNA |
| 0 |
0 |
0 |
3 |
15 |
NM_136257.2 |
CG8677-RA (CG8677), mRNA |
| 0 |
0 |
0 |
3 |
14 |
NM_175960.3 |
CG33196-RB (dp), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_078977.2 |
CG10897-RA, transcript variant A (tou), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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