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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 3268R-3 
 Symbol phtf  Full Name phtf 
 CG No CG3268  Old CG No CG3268 
 Synonyms CG3268, d-phtf, BcDNA.GH08636, BcDNA:GH08636, phtf 
 Accession No (Link to NCBI) NM_136388.2 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCCAGAAGAGAATCGGCACCTA 
 Primer Seq. 3' 
aatctagaggtaccTGGATGAGACTGATCAGCAAG 
 Predicted Fragment Size 
501 
 IR fragment full Seq 
0001 CCAGAAGAGA ATCGGCACCT ATGACAAGCA AGAATGGGAA AAGACCGTCG AACAGAGGAT 
0061 ATTGGACGGC TTCAATAGTG TCAATTTAAA AAACACCAAG CTGAAGACGG AGCTAATCGA 
0121 TGTGGACTTG GTGCGAGGTT CCACGTTCCC TAAGGCCAAG CCCAAGCAGT CGTTACTCAC 
0181 TGTGATACGC CTGGCCATTC TGCGCTATGT CCTGCTGCCC CTCTATGCCC AGTGGTGGGT 
0241 CAAGCAGACC ACGCCAAACG CCTTCGGCTT CATCCTTGTC CTTTACCTCA CACAGTTAAC 
0301 CAACTGGGCT ATCTACGTGC TTCACAGCAG TCGCATAGTG CCCCTTGACT ATGAGAAGCC 
0361 GCCAAATGGA ATCCTGCTTC AGGCAGAGGC AGATGGAGAT GCCTCCgata aggatgcaGA 
0421 TAAGGAGTCC GAGGAACATG CCGCCCTCCT CAGTGcCCTG CTTATTCCGT GCGCCCTAAG 
0481 CTTGCTGAtC AGTCTCATCC A 
 in silico PCR Fragment 
0001 CCAGAAGAGA ATCGGCACCT ATGACAAGCA AGAATGGGAA AAGACCGTCG AACAGAGGAT 
0061 ATTGGACGGC TTCAATAGTG TCAATTTAAA AAACACCAAG CTGAAGACGG AGCTAATCGA 
0121 TGTGGACTTG GTGCGAGGTT CCACGTTCCC TAAGGCCAAG CCCAAGCAGT CGTTACTCAC 
0181 TGTGATACGC CTGGCCATTC TGCGCTATGT CCTGCTGCCC CTCTATGCCC AGTGGTGGGT 
0241 CAAGCAGACC ACGCCAAACG CCTTCGGCTT CATCCTTGTG CTTTACCTCA CACAGTTAAC 
0301 CAACTGGGCT ATCTACGTGC TTCACAGCAG TCGCATAGTG CCCCTTGACT ATGAGAAGCC 
0361 GCCAAATGGA ACCCTGCTTC AGGCAGAGGC AGATGGAGAT GCCTCCGATA AGGATGCAGA 
0421 TAAGGAGTCC GAGGAACATG CCGCCCTCCT CAGTGCCCTG CTTATTCCGT GCGCCCTAAG 
0481 CTTGCTGATC AGTCTCATCC A 
 Assemble Data 

3268R-3.IR_full       1   CCAGAAGAGAATCGGCACCTATGACAAGCAAGAATGGGAAAAGACCGTCGAACAGAGGAT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3268R-3.in silico     1   CCAGAAGAGAATCGGCACCTATGACAAGCAAGAATGGGAAAAGACCGTCGAACAGAGGAT 60


3268R-3.IR_full       61  ATTGGACGGCTTCAATAGTGTCAATTTAAAAAACACCAAGCTGAAGACGGAGCTAATCGA 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3268R-3.in silico     61  ATTGGACGGCTTCAATAGTGTCAATTTAAAAAACACCAAGCTGAAGACGGAGCTAATCGA 120


3268R-3.IR_full       121 TGTGGACTTGGTGCGAGGTTCCACGTTCCCTAAGGCCAAGCCCAAGCAGTCGTTACTCAC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3268R-3.in silico     121 TGTGGACTTGGTGCGAGGTTCCACGTTCCCTAAGGCCAAGCCCAAGCAGTCGTTACTCAC 180


3268R-3.IR_full       181 TGTGATACGCCTGGCCATTCTGCGCTATGTCCTGCTGCCCCTCTATGCCCAGTGGTGGGT 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3268R-3.in silico     181 TGTGATACGCCTGGCCATTCTGCGCTATGTCCTGCTGCCCCTCTATGCCCAGTGGTGGGT 240


3268R-3.IR_full       241 CAAGCAGACCACGCCAAACGCCTTCGGCTTCATCCTTGTCCTTTACCTCACACAGTTAAC 300
                          ||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||
3268R-3.in silico     241 CAAGCAGACCACGCCAAACGCCTTCGGCTTCATCCTTGTGCTTTACCTCACACAGTTAAC 300


3268R-3.IR_full       301 CAACTGGGCTATCTACGTGCTTCACAGCAGTCGCATAGTGCCCCTTGACTATGAGAAGCC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3268R-3.in silico     301 CAACTGGGCTATCTACGTGCTTCACAGCAGTCGCATAGTGCCCCTTGACTATGAGAAGCC 360


3268R-3.IR_full       361 GCCAAATGGAATCCTGCTTCAGGCAGAGGCAGATGGAGATGCCTCCGATAAGGATGCAGA 420
                          ||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
3268R-3.in silico     361 GCCAAATGGAACCCTGCTTCAGGCAGAGGCAGATGGAGATGCCTCCGATAAGGATGCAGA 420


3268R-3.IR_full       421 TAAGGAGTCCGAGGAACATGCCGCCCTCCTCAGTGCCCTGCTTATTCCGTGCGCCCTAAG 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3268R-3.in silico     421 TAAGGAGTCCGAGGAACATGCCGCCCTCCTCAGTGCCCTGCTTATTCCGTGCGCCCTAAG 480


3268R-3.IR_full       481 CTTGCTGATCAGTCTCATCCA 501
                          |||||||||||||||||||||
3268R-3.in silico     481 CTTGCTGATCAGTCTCATCCA 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   483  0  0  0  NM_136388.2  CG3268-RA (phtf), mRNA 
0   0  5  5  2  NM_141441.1  CG14610-RA (CG14610), mRNA 
0   0  2  3  2  NM_137428.2  CG14489-RA (olf186-M), mRNA 
0   0  1  5  7  NM_206244.1  CG33232-RC, transcript variant C (CG33232), mRNA 
0   0  1  5  7  NM_206246.1  CG33232-RA, transcript variant A (CG33232), mRNA 
0   0  1  5  7  NM_206245.1  CG33232-RB, transcript variant B (CG33232), mRNA 
0   0  1  5  7  NM_001031933.1  CG33232-RD, transcript variant D (CG33232), mRNA 
0   0  1  4  8  NM_164578.1  CG15427-RA, transcript variant A (tutl), mRNA 
0   0  1  4  3  NM_140488.2  CG6945-RA (CG6945), mRNA 
0   0  1  4  3  NM_135493.2  CG13131-RA (CG13131), mRNA 
0   0  1  4  2  NM_143313.1  CG5639-RA (CG5639), mRNA 
0   0  1  3  3  NM_141526.2  CG9617-RA (CG9617), mRNA 
0   0  1  2  6  NM_079436.1  CG9617-RA (CG9617), mRNA, small, or homeotic discs 1 CG8887-RA (ash1), mRNA 
0   0  0  7  4  NM_057245.2  CG6189-RA (l(1)1Bi), mRNA 
0   0  0  5  3  NM_138760.2  CG5304-RA (l(2)01810), mRNA 
0   0  0  5  2  NM_079067.2  CG11949-RA, transcript variant A (cora), mRNA 
0   0  0  5  2  NM_166338.1  CG11949-RD, transcript variant D (cora), mRNA 
0   0  0  4  10  NM_170549.1  CG31204-RA (CG31204), mRNA 
0   0  0  4  9  NM_206684.2  CG1725-RE, transcript variant E (dlg1), mRNA 
0   0  0  4  9  NM_206682.2  CG1725-RG, transcript variant G (dlg1), mRNA 
0   0  0  4  9  NM_078565.3  CG1725-RD, transcript variant D (dlg1), mRNA 
0   0  0  4  9  NM_167282.2  CG1725-RA, transcript variant A (dlg1), mRNA 
0   0  0  4  8  NM_176556.1  CG33106-RA, transcript variant A (mask), mRNA 
0   0  0  4  8  NM_176557.1  CG33106-RB, transcript variant B (mask), mRNA 
0   0  0  4  7  NM_206681.2  CG1725-RH, transcript variant H (dlg1), mRNA 
0   0  0  4  7  NM_206683.2  CG1725-RB, transcript variant B (dlg1), mRNA 
0   0  0  4  4  NM_165534.1  CG1624-RB, transcript variant B (dpld), mRNA 
0   0  0  4  4  NM_165533.1  CG1624-RA, transcript variant A (dpld), mRNA 
0   0  0  4  4  NM_080033.2  CG1624-RC, transcript variant C (dpld), mRNA 
0   0  0  4  3  NM_137155.2  CG10228-RA (Pcf11), mRNA 

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