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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 3246R-2 
 Symbol CG3246  Full Name CG3246 
 CG No CG3246  Old CG No CG3246 
 Synonyms CG3246 
 Accession No (Link to NCBI) NM_134937.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGATAGTGGTCGTGCTCGTGGG 
 Primer Seq. 3' 
aatctagaggtaccTGGCCAGCTGACCGTCTCGTT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 NATAGTGGTC GTGCTCGTGG GCCTGATgGC AAGTGGCTGC CACATTGTTC ACTCCCAGGA 
0061 GCCTGGCGAT GCTGTGGAGC AGGAAGCCAG CGAGTCGGAT GACGCACTTA AGATCAAAGA 
0121 GAGTCAGGCC AGCATAGCCG CCCAGGTGGA CGGCCATGCT CGTGCACNTT TCACGCAGAG 
0181 ANGGATCCGC AGGGTCTGCC CGGCGTGCCC GTGCCNCGAT CCGTTGGAGG TGCCCAATGT 
0241 GAAGAAGAGC ATGGGCATGG CCAATCTGGA CATGAAGCAG GTCAAGGCGT ATGGCTTTGT 
0301 CCAAGTTCCG CATTGGTAAA ATGAACCTAG ACCTGAAGGA AATGAGATTC AACGGTGGCC 
0361 TCCAGCTGGA CCAGATGCTG GTCAAGGGCC AGTACACCCT CAGTTCATTC TTTTCGGAAA 
0421 GCCAATGGAC CCTTTACAGT AGTCCTGAAG AATGTCTATG NCGGAGGCAA CCGCCTTCTT 
0481 GGCCGTCGAA CGAGACGGTC ATGCTGGCCa  
 in silico PCR Fragment 
0001 GATAGTGGTC GTGCTCGTGG GCCTGATGGC AAGTGGCTGC CACATTGTTC ACTCCCAGGA 
0061 GCCTGGCGAT GCTGTGGAGC AGGAAGCCAG CGAGTCGGAT GACGCACTTA AGATCAAAGA 
0121 GAGTCAGGCC AGCATAGCCG CCCAGGTGGA -GGCCATGCT CGTGCAC-TT TCA-GCAG-G 
0181 A-GGATCCGC AGGGTCTGCC CGGCGTGCCC GTGCC-CGAT CCGTTGGAGG TGCCCAATGT 
0241 GAAGAAGAGC ATGGGCATGG CCAATCTGGA CATGAAGCAG GTCAAGGCGT ATGGC-TTGT 
0301 CCAAGTTCCG CATTGATAAA ATGAACCTAG ACCTGAAGGA AATGAGATTC AACGGTGGCC 
0361 TCCAGCTGGA CCAGATGCTG GTCAAGGGCC AGTACACCCT CAGTTCATTC TTTTC-GAAA 
0421 GCCAATGGAC CCTTTACAGT AGTCCTGAAG AATGTCTATG CC-GAGGCAA CCGCCTTCTT 
0481 GGCCGTCGAA CGAGACGGTC A-GCTGGCCA  
 Assemble Data 

3246R-2.IR_full        1   ATAGTGGTCGTGCTCGTGGGCCTGATGGCAAGTGGCTGCCACATTGTTCACTCCCAGGA 59
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3246R-2.in silico      1   ATAGTGGTCGTGCTCGTGGGCCTGATGGCAAGTGGCTGCCACATTGTTCACTCCCAGGA 59


3246R-2.IR_full       61  GCCTGGCGATGCTGTGGAGCAGGAAGCCAGCGAGTCGGATGACGCACTTAAGATCAAAGA 119
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3246R-2.in silico     61  GCCTGGCGATGCTGTGGAGCAGGAAGCCAGCGAGTCGGATGACGCACTTAAGATCAAAGA 119


3246R-2.IR_full       121 GAGTCAGGCCAGCATAGCCGCCCAGGTGGACGGCCATGCTCGTGCACNTTTCACGCAGAG 179
                          |||||||||||||||||||||||||||||| |||||||||||||||| ||||| |||| |
3246R-2.in silico     121 GAGTCAGGCCAGCATAGCCGCCCAGGTGGA-GGCCATGCTCGTGCAC-TTTCA-GCAG-G 179


3246R-2.IR_full       181 ANGGATCCGCAGGGTCTGCCCGGCGTGCCCGTGCCNCGATCCGTTGGAGGTGCCCAATGT 239
                          | ||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
3246R-2.in silico     181 A-GGATCCGCAGGGTCTGCCCGGCGTGCCCGTGCC-CGATCCGTTGGAGGTGCCCAATGT 239


3246R-2.IR_full       241 GAAGAAGAGCATGGGCATGGCCAATCTGGACATGAAGCAGGTCAAGGCGTATGGCTTTGT 299
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
3246R-2.in silico     241 GAAGAAGAGCATGGGCATGGCCAATCTGGACATGAAGCAGGTCAAGGCGTATGGC-TTGT 299


3246R-2.IR_full       301 CCAAGTTCCGCATTGGTAAAATGAACCTAGACCTGAAGGAAATGAGATTCAACGGTGGCC 359
                          ||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
3246R-2.in silico     301 CCAAGTTCCGCATTGATAAAATGAACCTAGACCTGAAGGAAATGAGATTCAACGGTGGCC 359


3246R-2.IR_full       361 TCCAGCTGGACCAGATGCTGGTCAAGGGCCAGTACACCCTCAGTTCATTCTTTTCGGAAA 419
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
3246R-2.in silico     361 TCCAGCTGGACCAGATGCTGGTCAAGGGCCAGTACACCCTCAGTTCATTCTTTTC-GAAA 419


3246R-2.IR_full       421 GCCAATGGACCCTTTACAGTAGTCCTGAAGAATGTCTATGNCGGAGGCAACCGCCTTCTT 479
                          |||||||||||||||||||||||||||||||||||||||| | |||||||||||||||||
3246R-2.in silico     421 GCCAATGGACCCTTTACAGTAGTCCTGAAGAATGTCTATGCC-GAGGCAACCGCCTTCTT 479


3246R-2.IR_full       481 GGCCGTCGAACGAGACGGTCATGCTGGCCA 509
                          ||||||||||||||||||||| ||||||||
3246R-2.in silico     481 GGCCGTCGAACGAGACGGTCA-GCTGGCCA 509



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_134937.2  CG3246-RA (CG3246), mRNA 
0   0  6  2  4  NM_168647.1  CG32156-RA, transcript variant A (Mbs), mRNA 
0   0  6  2  4  NM_168648.1  CG32156-RB, transcript variant B (Mbs), mRNA 
0   0  6  2  4  NM_168646.1  CG32156-RC, transcript variant C (Mbs), mRNA 
0   0  3  3  6  NM_140112.1  CG14165-RA (CG14165), mRNA 
0   0  3  3  2  NM_142825.2  CG12800-RA (Cyp6d4), mRNA 
0   0  3  3  2  NM_142989.3  CG6413-RA (Dis3), mRNA 
0   0  3  2  8  NM_130578.2  CG32809-RD, transcript variant D (CG32809), mRNA 
0   0  3  2  3  NM_166899.1  CG32809-RB, transcript variant B (CG32809), mRNA 
0   0  2  5  4  NM_141444.1  CG10061-RA (l(3)s2214), mRNA 
0   0  2  4  6  NM_057412.3  CG10223-RA (Top2), mRNA 
0   0  2  3  3  NM_141572.1  CG11768-RA (CG11768), mRNA 
0   0  2  3  2  NM_143267.2  CG6283-RA (CG6283), mRNA 
0   0  2  2  3  NM_169018.1  CG31530-RA (CG31530), mRNA 
0   0  2  2  3  NM_079616.2  CG7771-RA, transcript variant A (sim), mRNA 
0   0  2  2  3  NM_169495.2  CG7771-RB, transcript variant B (sim), mRNA 
0   0  2  2  3  NM_137771.1  CG4372-RA (CG4372), mRNA 
0   0  2  2  2  NM_167968.1  CG32846-RA (CG32846), mRNA 
0   0  1  3  3  NM_140179.1  CG11801-RA (CG11801), mRNA 
0   0  1  2  7  NM_176557.1  CG33106-RB, transcript variant B (mask), mRNA 
0   0  1  2  7  NM_176556.1  CG33106-RA, transcript variant A (mask), mRNA 
0   0  1  2  6  NM_058140.3  CG10033-RB, transcript variant B (for), mRNA 
0   0  1  2  6  NM_205906.1  CG10033-RH, transcript variant H (for), mRNA 
0   0  1  2  6  NM_205904.1  CG10033-RI, transcript variant I (for), mRNA 
0   0  1  2  6  NM_058139.3  CG10033-RA, transcript variant A (for), mRNA 
0   0  1  2  5  NM_058141.3  CG10033-RC, transcript variant C (for), mRNA 
0   0  1  2  5  NM_134319.3  CG10033-RD, transcript variant D (for), mRNA 
0   0  1  2  5  NM_058142.3  CG10033-RE, transcript variant E (for), mRNA 
0   0  1  2  5  NM_205907.1  CG10033-RG, transcript variant G (for), mRNA 
0   0  1  2  5  NM_001014464.1  CG10033-RJ, transcript variant J (for), mRNA 

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