NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 32453R-2

Symbol CG32453 Full Name CG32453

CG No CG32453 Old CG No CG32453

Synonyms CG32453

Accession No (Link to NCBI) NM_168954.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCGCTTCCTATTCGCTCTACT

Primer Seq. 3'

aatctagaggtaccGTTGATTGATCAGGCGACGCT

Predicted Fragment Size

IR fragment full Seq

0001 GCGCTTCCTA TTCGCTCTAC TTCTCAGCGT GCTCCTGTGT CTGCTCCTGG CTCAGGAGGG 
0061 CAGTAGCAGC ACATCTACAT CCTCGACGGC GACCACATCC ACCGACTCCT CTGCCACCAC 
0121 TACCACGGCT TCATCCGCCA CCACCACTAC CACCACTGCC TCCTCCGCAT CCACCACGAC 
0181 CACTGCCTCC TCTTTCCTCC TCCTCGGCGG AGGCCAGGAG GCGCAGACGT GCTCGCCGTC 
0241 GTCGCCTGGC TCGTGAGCGC CGTCGTCGCC AGGAGCGGAG ACAGCGCCAG GAAAAGAGGA 
0301 GGCGCAGGAT GGAGCAGCTG CTGGTGAGGC AGCGTCGCCT GATCAATCAA C

in silico PCR Fragment

0001 GCGCTTCCTA TTCGCTCTAC TTCTCAGCGT GCTCCTGTGT CTGCTCCTGG CTCAGGAGGG 
0061 CAGTAGCAGC ACATCTACAT CCTCGACGGC GACCACATCC ACCGACTCCT CTGCCACCAC 
0121 TACCACGGCT TCATCCGCCA CCACCACTAC CACCACTGCC TCCTCCGCAT CCACCACGAC 
0181 CACTGCCTCC TC-TTCCTCC TCCTCGGCGG AGGCCAGGAG GCGCAGACGT GCTCGCCGTC 
0241 GTCGCCTGGC TCGTGAGCGC CGTCGTCGCC AGGAGCGGAG ACAGCGCCAG GAAAAGAGGA 
0301 GGCGCAGGAT GGAGCAGCTG CTGGTGAGGC AGCGTCGCCT GATCAATCAA C

Assemble Data


32453R-2.IR_full       1   GCGCTTCCTATTCGCTCTACTTCTCAGCGTGCTCCTGTGTCTGCTCCTGGCTCAGGAGGG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32453R-2.in silico     1   GCGCTTCCTATTCGCTCTACTTCTCAGCGTGCTCCTGTGTCTGCTCCTGGCTCAGGAGGG 60


32453R-2.IR_full       61  CAGTAGCAGCACATCTACATCCTCGACGGCGACCACATCCACCGACTCCTCTGCCACCAC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32453R-2.in silico     61  CAGTAGCAGCACATCTACATCCTCGACGGCGACCACATCCACCGACTCCTCTGCCACCAC 120


32453R-2.IR_full       121 TACCACGGCTTCATCCGCCACCACCACTACCACCACTGCCTCCTCCGCATCCACCACGAC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32453R-2.in silico     121 TACCACGGCTTCATCCGCCACCACCACTACCACCACTGCCTCCTCCGCATCCACCACGAC 180


32453R-2.IR_full       181 CACTGCCTCCTCTTTCCTCCTCCTCGGCGGAGGCCAGGAGGCGCAGACGTGCTCGCCGTC 240
                           |||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
32453R-2.in silico     181 CACTGCCTCCTC-TTCCTCCTCCTCGGCGGAGGCCAGGAGGCGCAGACGTGCTCGCCGTC 240


32453R-2.IR_full       241 GTCGCCTGGCTCGTGAGCGCCGTCGTCGCCAGGAGCGGAGACAGCGCCAGGAAAAGAGGA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32453R-2.in silico     241 GTCGCCTGGCTCGTGAGCGCCGTCGTCGCCAGGAGCGGAGACAGCGCCAGGAAAAGAGGA 300


32453R-2.IR_full       301 GGCGCAGGATGGAGCAGCTGCTGGTGAGGCAGCGTCGCCTGATCAATCAAC 351
                           |||||||||||||||||||||||||||||||||||||||||||||||||||
32453R-2.in silico     301 GGCGCAGGATGGAGCAGCTGCTGGTGAGGCAGCGTCGCCTGATCAATCAAC 351

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	332	0	10	44	NM_168954.1	CG32453-RA (CG32453), mRNA
99.39	330	2	10	44	NM_141147.2	CG14454-RA (CG14454), mRNA
15.36	51	68	46	53	NM_141150.1	CG14452-RA (CG14452), mRNA
15.36	51	68	46	53	NM_141148.1	CG12546-RA (CG12546), mRNA
0.6	2	8	36	97	NM_001014738.1	CG7107-RF, transcript variant F (up), mRNA
0.6	2	8	36	92	NM_167375.1	CG7107-RB, transcript variant B (up), mRNA
0.6	2	8	36	92	NM_080349.2	CG7107-RA, transcript variant A (up), mRNA
0.6	2	8	36	92	NM_001014737.1	CG7107-RG, transcript variant G (up), mRNA
0.6	2	8	36	91	NM_167376.1	CG7107-RD, transcript variant D (up), mRNA
0.6	2	8	36	91	NM_001014739.1	CG7107-RE, transcript variant E (up), mRNA
0.6	2	4	2	13	NM_130578.2	CG32809-RD, transcript variant D (CG32809), mRNA
0.6	2	2	9	5	NM_137628.1	CG13868-RA (CG13868), mRNA
0.3	1	9	28	59	NM_137672.1	CG15225-RA (CG15225), mRNA
0.3	1	5	17	50	NM_132012.1	CG15776-RA (CG15776), mRNA
0.3	1	4	20	51	NM_132346.1	CG3003-RB (CG3003), mRNA
0.3	1	4	8	25	NM_166141.2	CG8421-RD, transcript variant D (Asph), mRNA
0.3	1	4	8	25	NM_176185.1	CG8421-RE, transcript variant E (Asph), mRNA
0.3	1	4	5	20	NM_079033.2	CG8421-RB, transcript variant B (Asph), mRNA
0.3	1	4	5	20	NM_166140.1	CG8421-RA, transcript variant A (Asph), mRNA
0.3	1	3	12	26	NM_080335.2	CG2984-RA (Pp2C1), mRNA
0.3	1	3	4	5	NM_139378.2	CG13928-RA (CG13928), mRNA
0.3	1	2	9	23	NM_143060.1	CG13647-RA (CG13647), mRNA
0.3	1	2	8	11	NM_001014745.1	CG9210-RB, transcript variant B (Ac13E), mRNA
0.3	1	2	8	11	NM_057951.2	CG9210-RA, transcript variant A (Ac13E), mRNA
0.3	1	2	4	16	NM_131987.2	CG4202-RA (Sas10), mRNA
0	0	7	20	43	NM_167524.2	CG9819-RB, transcript variant B (CanA-14F), mRNA
0	0	7	20	43	NM_167523.2	CG9819-RA, transcript variant A (CanA-14F), mRNA
0	0	6	19	38	NM_206663.1	CG12075-RB, transcript variant B (CG12075), mRNA
0	0	6	19	38	NM_132278.2	CG12075-RA, transcript variant A (CG12075), mRNA
0	0	6	17	52	NM_170053.2	CG17894-RC, transcript variant C (cnc), mRNA

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