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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 32406R-3 
 Symbol CG32406  Full Name CG32406 
 CG No CG32406  Old CG No CG32406 
 Synonyms CG6062, CG13292, CG32406 
 Accession No (Link to NCBI) NM_168153.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAGCGAGGCCGCCACAATGATA 
 Primer Seq. 3' 
aatctagaggtaccTGCGTGAAGAGTTGCAGTGGG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AGCGAGGCCG CCACAATGAT AACCAACAAT AATTTGGATT ATGACCAGGC CAGCGCCAGC 
0061 AGCACAATAA TAGCAGGAGC TACATCGACG ACCGCGGGCA CAGCGGCAAC ATCGGTGGCC 
0121 ATGAGCAACA GAAATGGCAG CACCAGCAGC AGCAGCAACT CCTCGCCCAC GGCGGACNAA 
0181 TTCCAATTCC AATTCCAACT CGAATTCAAA CGCAAATATA AATCGGAATT TGAATCAGAA 
0241 CCCCAACTCG AACTCCAATA CAAACTCCAA TTCGTATTTC AACCGATTCG ACAATCGCAT 
0301 AGCTGCCAAC TTGGCTGCCG TTTTAACTGG CGCAGGAGCC CGTTTTCGAT CCTCCTCCTG 
0361 CAGCAATCGC CATCCAGCTG GAGTTGCAAA TCCGGCCGTT TTGAGATCAC CACTCGCCAC 
0421 AATAAGCCGC AAGACTAGTC TGGCCACGCC CCCTTCACCA GCGAGTCTAG GACGACGCCG 
0481 CCCACTGCAA CTCTTCACGC A 
 in silico PCR Fragment 
0001 AGCGAGGCCG CCACAATGAT AACCAACAAT AATTTGGATT ATGACCAGGC CAGCGCCAGC 
0061 AGCACAATAA TAGCAGGAGC TACATCGACG ACCGCGGGCA CAGCGGCAAC ATCGGTGGCC 
0121 ATGAGCAACA GAAATGGCAG CACCAGCAGC AGCAGCAACT CCTCGCCCAC GGCGGAC-AA 
0181 TTCCAATTCC AATTCCAACT CGAATTCAAA CGCAAATATA AATCGGAATT TGAATCAGAA 
0241 CCCCAACTCG AACTCCAATA CAAACTCCAA TTCGTATTTC AACCGATTCG ACAATCGCAT 
0301 AGCTGCCAAC TTGGCTGCCG TTTTAACTGG CGCAGGAGCC CGTTTTCGAT CCTCCTCCTG 
0361 CAGCAATCGC CATCCAGCTG GAGTTGCAAA TCCGGCCGTT TTGAGATCAC CACTCGCCAC 
0421 AATAAGCCGC AAGACTAGTC TGGCCACGCC CCCTTCACCA GCGAGTCTAG GACGACGCCG 
0481 CCCACTGCAA CTCTTCACGC A 
 Assemble Data 

32406R-3.IR_full       1   AGCGAGGCCGCCACAATGATAACCAACAATAATTTGGATTATGACCAGGCCAGCGCCAGC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32406R-3.in silico     1   AGCGAGGCCGCCACAATGATAACCAACAATAATTTGGATTATGACCAGGCCAGCGCCAGC 60


32406R-3.IR_full       61  AGCACAATAATAGCAGGAGCTACATCGACGACCGCGGGCACAGCGGCAACATCGGTGGCC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32406R-3.in silico     61  AGCACAATAATAGCAGGAGCTACATCGACGACCGCGGGCACAGCGGCAACATCGGTGGCC 120


32406R-3.IR_full       121 ATGAGCAACAGAAATGGCAGCACCAGCAGCAGCAGCAACTCCTCGCCCACGGCGGACNAA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||
32406R-3.in silico     121 ATGAGCAACAGAAATGGCAGCACCAGCAGCAGCAGCAACTCCTCGCCCACGGCGGAC-AA 180


32406R-3.IR_full       181 TTCCAATTCCAATTCCAACTCGAATTCAAACGCAAATATAAATCGGAATTTGAATCAGAA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32406R-3.in silico     181 TTCCAATTCCAATTCCAACTCGAATTCAAACGCAAATATAAATCGGAATTTGAATCAGAA 240


32406R-3.IR_full       241 CCCCAACTCGAACTCCAATACAAACTCCAATTCGTATTTCAACCGATTCGACAATCGCAT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32406R-3.in silico     241 CCCCAACTCGAACTCCAATACAAACTCCAATTCGTATTTCAACCGATTCGACAATCGCAT 300


32406R-3.IR_full       301 AGCTGCCAACTTGGCTGCCGTTTTAACTGGCGCAGGAGCCCGTTTTCGATCCTCCTCCTG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32406R-3.in silico     301 AGCTGCCAACTTGGCTGCCGTTTTAACTGGCGCAGGAGCCCGTTTTCGATCCTCCTCCTG 360


32406R-3.IR_full       361 CAGCAATCGCCATCCAGCTGGAGTTGCAAATCCGGCCGTTTTGAGATCACCACTCGCCAC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32406R-3.in silico     361 CAGCAATCGCCATCCAGCTGGAGTTGCAAATCCGGCCGTTTTGAGATCACCACTCGCCAC 420


32406R-3.IR_full       421 AATAAGCCGCAAGACTAGTCTGGCCACGCCCCCTTCACCAGCGAGTCTAGGACGACGCCG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32406R-3.in silico     421 AATAAGCCGCAAGACTAGTCTGGCCACGCCCCCTTCACCAGCGAGTCTAGGACGACGCCG 480


32406R-3.IR_full       481 CCCACTGCAACTCTTCACGCA 501
                           |||||||||||||||||||||
32406R-3.in silico     481 CCCACTGCAACTCTTCACGCA 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  8  28  NM_168153.1  CG32406-RA (CG32406), mRNA 
1.03   5  21  79  192  NM_057771.2  CG1864-RB, transcript variant B (Hr38), mRNA 
1.03   5  12  26  62  NM_057894.3  CG5799-RA, transcript variant A (dve), mRNA 
1.03   5  6  46  114  NM_135793.2  CG16970-RA (CG16970), mRNA 
1.03   5  6  28  77  NM_142180.2  CG4285-RA (CG4285), mRNA 
1.03   5  6  14  15  NM_165334.1  CG2488-RA, transcript variant A (phr6-4), mRNA 
1.03   5  6  14  15  NM_057840.3  CG2488-RB, transcript variant B (phr6-4), mRNA 
1.03   5  5  29  75  NM_057502.2  CG1322-RB, transcript variant B (zfh1), mRNA 
1.03   5  5  26  71  NM_170522.2  CG1322-RC, transcript variant C (zfh1), mRNA 
1.03   5  5  17  36  NM_137930.2  CG3124-RA (CG3124), mRNA 
1.03   5  2  18  45  NM_057385.4  CG11121-RA (so), mRNA 
1.03   5  2  13  33  NM_170523.2  CG1322-RA, transcript variant A (zfh1), mRNA 
1.03   5  2  11  29  NM_080325.3  CG6450-RC (lva), mRNA 
0.82   4  55  173  407  NM_166030.1  CG8118-RB, transcript variant B (mam), mRNA 
0.82   4  55  173  407  NM_080376.1  CG8118-RA, transcript variant A (mam), mRNA 
0.82   4  27  81  174  NM_079507.2  CG2530-RA (corto), mRNA 
0.82   4  6  24  52  NM_206109.1  CG8118-RC, transcript variant C (mam), mRNA 
0.82   4  3  9  25  NM_137583.3  CG11228-RA (hpo), mRNA 
0.62   3  10  31  37  NM_132636.2  CG12723-RA (CG12723), mRNA 
0.62   3  8  33  125  NM_167744.3  CG1693-RB, transcript variant B (tty), mRNA 
0.62   3  8  33  125  NM_080712.5  CG1693-RA, transcript variant A (tty), mRNA 
0.62   3  6  52  120  NM_142259.2  CG10278-RA (GATAe), mRNA 
0.62   3  6  14  20  NM_169285.3  CG8144-RC, transcript variant C (ps), mRNA 
0.62   3  6  14  20  NM_001043326.1  CG8144-RI, transcript variant I (ps), mRNA 
0.62   3  6  14  20  NM_169284.2  CG8144-RD, transcript variant D (ps), mRNA 
0.62   3  5  18  23  NM_134555.1  CG15454-RA (CG15454), mRNA 
0.62   3  4  46  116  NM_001038740.1  CG32776-RD, transcript variant D (CG32776), mRNA 
0.62   3  4  46  116  NM_206622.2  CG32776-RB, transcript variant B (CG32776), mRNA 
0.62   3  3  32  75  NM_166996.2  CG32776-RA, transcript variant A (CG32776), mRNA 
0.62   3  3  32  75  NM_001038741.1  CG32776-RC, transcript variant C (CG32776), mRNA 

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