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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
32373R-4 |
| Symbol |
CG32373 |
Full Name |
CG32373 |
| CG No |
CG32373 |
Old CG No |
CG32373 |
| Synonyms |
BcDNA:RE64043, CG32373 |
| Accession No (Link to NCBI) |
NM_168229.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAATACGACGATACCTGCTGGA |
| Primer Seq. 3' |
aatctagaggtaccGGGATCAGCGCACTCATCAAT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AATACGACGA TACCTGCTGG ACTCCTCCGA GGATTAAAAA TGCTAAGGCA TCCGTCAAAC
0061 TTCGCCGAAG TGGGGAACAT GTCTTTCTTG TGACCTATTA TTCCTGCCGC GTCAACTACA
0121 AACTCAAACG TGCCAAAGAT AATGCACTCT ATTGCTCAAA TGGTATATGG CTTGGTTTGA
0181 AACCCAATTG CATAAAGATT GGTAAAGGAA ACACGCATTC AATGAAGCAA AAAAAAAATG
0241 AGATGCAGGA TTGATAATGG TGGTTGCGCT CATATATGTA ACAGATCAAC TCATAAATGC
0301 GAATGCTATG AGGGTTATAC ACTGAATTCC ACCGATTTAA GAAGCTGTAA TGATATCGAC
0361 GAGTGCAAGG AATCGAATGG CGGCTGCTCT CAAGTTTGCA ACAACCTTCC TGGTGAATTC
0421 ATATGCACCT GTAATAGTGG TTTTGAAATA GACGAAAGTG ATGAAAAAAC TTGCTTGGAC
0481 ATTGATGAGT GCGCTGATCC C |
| in silico PCR Fragment |
0001 AATACGACGA TACCTGCTGG ACTCCTCCGA GGATTAAAAA TGCTAAGGCA TCCGTCAAAC
0061 TTCGCCGAAA TGGGGAACAT GTCTTTCTTG TGACCTATTA TTCCTGCCGC GTCAACTACA
0121 AACTCAAACG TGCCAAAGAT AATGCACTCT ATTGCTCAAA TGGTATATGG CTTGGTTTGA
0181 AACCCAATTG CATAAAGATT GGTAAAGGAA ACACGCATTC AATGAAGC-A AAAAAAAATG
0241 AGATGCAGGA TTGATAATGG TGGTTGCGCT CATATATGTA ACAGATCAAC TCATAAATGC
0301 GAATGCTATG AGGGTTATAC ACTGAATTCC ACCGATTTAA GAAGCTGTAA TGATATCGAC
0361 GAGTGCAAGG AATCGAATGG CGGCTGCTCT CAAGTTTGCA ACAACCTTCC TGGTGAATTC
0421 ATATGCACCT GTAATAGTGG TTTTGAAATA GACGAAAGTG ATGAAAAAAC TTGCTTGGAC
0481 ATTGATGAGT GCGCTGATCC C |
| Assemble Data |
32373R-4.IR_full 1 AATACGACGATACCTGCTGGACTCCTCCGAGGATTAAAAATGCTAAGGCATCCGTCAAAC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32373R-4.in silico 1 AATACGACGATACCTGCTGGACTCCTCCGAGGATTAAAAATGCTAAGGCATCCGTCAAAC 60
32373R-4.IR_full 61 TTCGCCGAAGTGGGGAACATGTCTTTCTTGTGACCTATTATTCCTGCCGCGTCAACTACA 120
||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
32373R-4.in silico 61 TTCGCCGAAATGGGGAACATGTCTTTCTTGTGACCTATTATTCCTGCCGCGTCAACTACA 120
32373R-4.IR_full 121 AACTCAAACGTGCCAAAGATAATGCACTCTATTGCTCAAATGGTATATGGCTTGGTTTGA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32373R-4.in silico 121 AACTCAAACGTGCCAAAGATAATGCACTCTATTGCTCAAATGGTATATGGCTTGGTTTGA 180
32373R-4.IR_full 181 AACCCAATTGCATAAAGATTGGTAAAGGAAACACGCATTCAATGAAGCAAAAAAAAAATG 240
|||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
32373R-4.in silico 181 AACCCAATTGCATAAAGATTGGTAAAGGAAACACGCATTCAATGAAGC-AAAAAAAAATG 240
32373R-4.IR_full 241 AGATGCAGGATTGATAATGGTGGTTGCGCTCATATATGTAACAGATCAACTCATAAATGC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32373R-4.in silico 241 AGATGCAGGATTGATAATGGTGGTTGCGCTCATATATGTAACAGATCAACTCATAAATGC 300
32373R-4.IR_full 301 GAATGCTATGAGGGTTATACACTGAATTCCACCGATTTAAGAAGCTGTAATGATATCGAC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32373R-4.in silico 301 GAATGCTATGAGGGTTATACACTGAATTCCACCGATTTAAGAAGCTGTAATGATATCGAC 360
32373R-4.IR_full 361 GAGTGCAAGGAATCGAATGGCGGCTGCTCTCAAGTTTGCAACAACCTTCCTGGTGAATTC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32373R-4.in silico 361 GAGTGCAAGGAATCGAATGGCGGCTGCTCTCAAGTTTGCAACAACCTTCCTGGTGAATTC 420
32373R-4.IR_full 421 ATATGCACCTGTAATAGTGGTTTTGAAATAGACGAAAGTGATGAAAAAACTTGCTTGGAC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32373R-4.in silico 421 ATATGCACCTGTAATAGTGGTTTTGAAATAGACGAAAGTGATGAAAAAACTTGCTTGGAC 480
32373R-4.IR_full 481 ATTGATGAGTGCGCTGATCCC 501
|||||||||||||||||||||
32373R-4.in silico 481 ATTGATGAGTGCGCTGATCCC 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_206297.1 |
CG32373-RB, transcript variant B (CG32373), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_168229.1 |
CG32373-RA, transcript variant A (CG32373), mRNA |
| 0.62 |
3 |
10 |
9 |
54 |
NM_139880.2 |
CG7526-RA, transcript variant A (CG7526), mRNA |
| 0.62 |
3 |
10 |
9 |
54 |
NM_206292.1 |
CG7526-RB, transcript variant B (CG7526), mRNA |
| 0.2 |
1 |
3 |
3 |
2 |
NM_136800.1 |
CG13218-RA (CG13218), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_079756.2 |
CG6383-RA, transcript variant A (crb), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_001043286.1 |
CG6383-RB, transcript variant B (crb), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_141561.2 |
CG8116-RA (CG8116), mRNA |
| 0 |
0 |
1 |
11 |
33 |
NM_175960.3 |
CG33196-RB (dp), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_058096.3 |
CG8725-RA (CSN4), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_169222.2 |
CG31462-RA (CG31462), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_142913.1 |
CG10254-RA, transcript variant A (CG10254), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_170081.1 |
CG10254-RB, transcript variant B (CG10254), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_167402.1 |
CG1517-RC, transcript variant C (na), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_167401.1 |
CG1517-RB, transcript variant B (na), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_164890.1 |
CG4839-RB, transcript variant B (CG4839), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_135505.2 |
CG4839-RA, transcript variant A (CG4839), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_141264.2 |
CG12163-RB, transcript variant B (CG12163), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_169033.2 |
CG12163-RA, transcript variant A (CG12163), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_206293.1 |
CG33275-RC, transcript variant C (CG33275), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_206295.1 |
CG33275-RB, transcript variant B (CG33275), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_078693.2 |
CG9565-RA (Nep3), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_206294.1 |
CG33275-RA, transcript variant A (CG33275), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_139442.1 |
CG15878-RA (CG15878), mRNA |
| 0 |
0 |
0 |
7 |
11 |
NM_166745.1 |
CG31999-RA (CG31999), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_143141.2 |
CG4548-RB, transcript variant B (XNP), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_170228.1 |
CG4548-RA, transcript variant A (XNP), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_141972.1 |
CG5999-RA (CG5999), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_169082.1 |
CG31551-RA (CG31551), mRNA |
| 0 |
0 |
0 |
3 |
9 |
NM_078740.2 |
CG8817-RB, transcript variant B (lilli), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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