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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

32219R-3

Symbol

CG32219

Full Name

CG32219

CG No

CG32219

Old CG No

CG32219

Synonyms

BcDNA:AT25132, CG32219

Accession No (Link to NCBI)

NM_168827.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCGGCTGTCGAAGATCATTACG

Primer Seq. 3'

aatctagaggtaccCAACCGATCAGCAGTAATGAG

Predicted Fragment Size

IR fragment full Seq

0001 CGGCTGTCGA AGATCATTAC GATTGTGGAT GTTGTCCGGG CATAAGTAAC AACAATGGCC 
0061 AGGTGGTTGG GTTCCATTGC CATAAAGATG ACCAAAACTC AATTTTAAGA TTTGCATCGG 
0121 ATGAGGAAAG TGCGTTTGCT GAATGTGTGC CAAACTGTTT TGAGATAAAA TATCCCATTC 
0181 AGGAATACTG CTGTTTCTGG TCACCGAAGC TGGGCTGTAC GATACTAGTC AATGAAAAGT 
0241 ATTTCAGCAA TAAAGACTTG TGCAATAACT GCAAGTATTT TTGCCAAAGA ACTACAGGAG 
0301 ATGGGAGGAG CTCTAGTTAT CCACAGAAAC TTATCTTGCT ACTCTCATTA CTGCTGATCG 
0361 GTTG

in silico PCR Fragment

0001 CGGCTGTCGA AGATCATTAC GATTGTGGAT GTTGTCCGGG CATAAGTAAC AACAATGGCC 
0061 AGGTGGTTGG GTTCCATTGC CATAAAGATG ACCAAAACTC AATTTTAAGA TTTGCATCGG 
0121 ATGAGGAAAG TGCGTTTGCT GAATGTGTGC CAAACTGTTT TGAGATAAAA TATCCCATTC 
0181 AGGAATACTG CTGTTTCTGG TCACCGAAGC TGGGCTGTAC GATACTAGTC AATGAAAAGT 
0241 ATTTCAGCAA TAAAGACTTG TGCAATAACT GCAAGTATTT TTGCCAAAGA ACTACAGGAG 
0301 ATGGGAGGAG CTCTAGTTAT CCACAGAAAC TTATCTTGCT ACTCTCATTA CTGCTGATCG 
0361 GTTG

Assemble Data


32219R-3.IR_full       1   CGGCTGTCGAAGATCATTACGATTGTGGATGTTGTCCGGGCATAAGTAACAACAATGGCC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32219R-3.in silico     1   CGGCTGTCGAAGATCATTACGATTGTGGATGTTGTCCGGGCATAAGTAACAACAATGGCC 60


32219R-3.IR_full       61  AGGTGGTTGGGTTCCATTGCCATAAAGATGACCAAAACTCAATTTTAAGATTTGCATCGG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32219R-3.in silico     61  AGGTGGTTGGGTTCCATTGCCATAAAGATGACCAAAACTCAATTTTAAGATTTGCATCGG 120


32219R-3.IR_full       121 ATGAGGAAAGTGCGTTTGCTGAATGTGTGCCAAACTGTTTTGAGATAAAATATCCCATTC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32219R-3.in silico     121 ATGAGGAAAGTGCGTTTGCTGAATGTGTGCCAAACTGTTTTGAGATAAAATATCCCATTC 180


32219R-3.IR_full       181 AGGAATACTGCTGTTTCTGGTCACCGAAGCTGGGCTGTACGATACTAGTCAATGAAAAGT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32219R-3.in silico     181 AGGAATACTGCTGTTTCTGGTCACCGAAGCTGGGCTGTACGATACTAGTCAATGAAAAGT 240


32219R-3.IR_full       241 ATTTCAGCAATAAAGACTTGTGCAATAACTGCAAGTATTTTTGCCAAAGAACTACAGGAG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32219R-3.in silico     241 ATTTCAGCAATAAAGACTTGTGCAATAACTGCAAGTATTTTTGCCAAAGAACTACAGGAG 300


32219R-3.IR_full       301 ATGGGAGGAGCTCTAGTTATCCACAGAAACTTATCTTGCTACTCTCATTACTGCTGATCG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32219R-3.in silico     301 ATGGGAGGAGCTCTAGTTATCCACAGAAACTTATCTTGCTACTCTCATTACTGCTGATCG 360


32219R-3.IR_full       361 GTTG 364
                           ||||
32219R-3.in silico     361 GTTG 364

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	346	0	0	0	NM_168827.1	CG32219-RA (CG32219), mRNA
0	0	3	3	3	NM_167012.1	CG7035-RA, transcript variant A (Cbp80), mRNA
0	0	3	3	3	NM_080011.2	CG7035-RB, transcript variant B (Cbp80), mRNA
0	0	1	2	3	NM_079007.2	CG17716-RA (fas), mRNA
0	0	1	2	3	NM_166735.1	CG31997-RA (CG31997), mRNA
0	0	0	5	6	NM_168384.1	CG6718-RC, transcript variant C (CG6718), mRNA
0	0	0	5	6	NM_168385.1	CG6718-RD, transcript variant D (CG6718), mRNA
0	0	0	4	3	NM_142394.1	CG16766-RA (CG16766), mRNA
0	0	0	4	3	NM_001032076.1	CG33694-RA, transcript variant A (cana), mRNA
0	0	0	4	3	NM_001032080.1	CG33695-RA, transcript variant A (CG33695), mRNA
0	0	0	4	3	NM_132881.2	CG3415-RA (CG3415), mRNA
0	0	0	4	2	NM_166163.1	CG8048-RC, transcript variant C (Vha44), mRNA
0	0	0	4	2	NM_166164.2	CG8048-RD, transcript variant D (Vha44), mRNA
0	0	0	4	2	NM_134313.2	CG8048-RB, transcript variant B (Vha44), mRNA
0	0	0	4	2	NM_057918.3	CG8048-RA, transcript variant A (Vha44), mRNA
0	0	0	3	8	NM_142210.1	CG6118-RA (CG6118), mRNA
0	0	0	3	6	NM_170215.1	CG31288-RA (CG31288), mRNA
0	0	0	3	6	NM_078581.2	CG1500-RA (fw), mRNA
0	0	0	3	5	NM_080376.1	CG8118-RA, transcript variant A (mam), mRNA
0	0	0	3	5	NM_166030.1	CG8118-RB, transcript variant B (mam), mRNA
0	0	0	3	5	NM_001014453.1	CG33526-RD, transcript variant D (PNUTS), mRNA
0	0	0	3	4	NM_165528.1	CG11140-RB, transcript variant B (Aldh-III), mRNA
0	0	0	3	4	NM_165526.1	CG11140-RI, transcript variant I (Aldh-III), mRNA
0	0	0	3	4	NM_165527.1	CG11140-RA, transcript variant A (Aldh-III), mRNA
0	0	0	3	4	NM_135693.1	CG6766-RA (CG6766), mRNA
0	0	0	3	3	NM_176477.1	CG3359-RM, transcript variant M (mfas), mRNA
0	0	0	3	3	NM_176473.1	CG3359-RE, transcript variant E (mfas), mRNA
0	0	0	3	3	NM_176469.1	CG3359-RL, transcript variant L (mfas), mRNA
0	0	0	3	3	NM_079600.2	CG3359-RC, transcript variant C (mfas), mRNA
0	0	0	3	3	NM_176474.1	CG3359-RO, transcript variant O (mfas), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

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