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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 32107R-1 
 Symbol CG32107  Full Name CG32107 
 CG No CG32107  Old CG No CG32107 
 Synonyms CG32107 
 Accession No (Link to NCBI) NM_168526.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAAAGAGGTAGATGCATCCGAG 
 Primer Seq. 3' 
aatctagaggtaccGCATCATACCAGCCTATGCAA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AAAGAGGTAG ATGCATCCGA GTACGTAGCC CTGGCCAATT TGCCGCAACT TAAGTCATTA 
0061 ATTCTGTGTG GCGAGCATCT CGAGGGATCG CTGGTGAAGC TTTTTAATCG CTTCCAAGAA 
0121 CACCAGCACA TCCAGAGCAT ATGCATCCCG GAAACATATG TCAGCAATGC AGAGGCCATT 
0181 GCCTTGGCAT CCATTCGCTC AGTGGTTTCA ATTAAGTGCT GTTTGCGGGA TAAATCGATC 
0241 TATTCGGACT TGCCGTTAAG CGGAAAACTT ACTCATATCT ACATTCTGGG TCACCCCATG 
0301 CAATATAATC CAGACAATCC GTATTACAAT GAAAAGGAGG AGGATTTTCT CAAAGTGACT 
0361 TATAGGGCAC ATAGACCGTT AGCCGTCGTG CGTGCATATT ATCAATATTG GCAGACCAAA 
0421 GATCCAAATC TATTGCAATT GGCCTTCGAT GAAGATTATT TGCATGCACA TCGTCTCTAT 
0481 TGCATAGGCT GGTATGATGC  
 in silico PCR Fragment 
0001 AAAGAGGTAG ATGCATCCGA GTACGTAGCC CTGGCCAATT TGCCGCAACT TAAGTCATTA 
0061 ATTCTGTGTG GCGAGCATCT CGAGGGATCG CTTGTGAAGC TTTTTAATCG CTTCCAAGAA 
0121 CACCAGCACA TCCAGAGCAT ATGTATCCCG GAAACATATG TCAGCAATGC AGAGGCCATC 
0181 GCCTTGGCAT CCATTCGCTT AGTGGTTTCA ATTAAGTGCT GTTTGCGGGA TAAATCGATC 
0241 TATTCGGACT TGCCGTTAAG CGGAAAACTT ACTCATATCT ACATTCTGGG TCACCCCATG 
0301 CAATATAATC CAGACAATCC GTATTACAAT GAAAAGGAGG AGGATTTTCT CAAAGTGACT 
0361 TATAGGGCAC ATAGACCGTT AGCCGTCGTG CGTGCATATT ATCAATATTG GCAGACCAAA 
0421 GATCCAAATC TATTGCAATT GGCCTTCGAT GAAGATTATT TGCATGCACA TCGTCTCTAT 
0481 TGCATAGGCT GGTATGATGC  
 Assemble Data 

32107R-1.IR_full       1   AAAGAGGTAGATGCATCCGAGTACGTAGCCCTGGCCAATTTGCCGCAACTTAAGTCATTA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32107R-1.in silico     1   AAAGAGGTAGATGCATCCGAGTACGTAGCCCTGGCCAATTTGCCGCAACTTAAGTCATTA 60


32107R-1.IR_full       61  ATTCTGTGTGGCGAGCATCTCGAGGGATCGCTGGTGAAGCTTTTTAATCGCTTCCAAGAA 120
                           |||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
32107R-1.in silico     61  ATTCTGTGTGGCGAGCATCTCGAGGGATCGCTTGTGAAGCTTTTTAATCGCTTCCAAGAA 120


32107R-1.IR_full       121 CACCAGCACATCCAGAGCATATGCATCCCGGAAACATATGTCAGCAATGCAGAGGCCATT 180
                           ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||| 
32107R-1.in silico     121 CACCAGCACATCCAGAGCATATGTATCCCGGAAACATATGTCAGCAATGCAGAGGCCATC 180


32107R-1.IR_full       181 GCCTTGGCATCCATTCGCTCAGTGGTTTCAATTAAGTGCTGTTTGCGGGATAAATCGATC 240
                           ||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
32107R-1.in silico     181 GCCTTGGCATCCATTCGCTTAGTGGTTTCAATTAAGTGCTGTTTGCGGGATAAATCGATC 240


32107R-1.IR_full       241 TATTCGGACTTGCCGTTAAGCGGAAAACTTACTCATATCTACATTCTGGGTCACCCCATG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32107R-1.in silico     241 TATTCGGACTTGCCGTTAAGCGGAAAACTTACTCATATCTACATTCTGGGTCACCCCATG 300


32107R-1.IR_full       301 CAATATAATCCAGACAATCCGTATTACAATGAAAAGGAGGAGGATTTTCTCAAAGTGACT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32107R-1.in silico     301 CAATATAATCCAGACAATCCGTATTACAATGAAAAGGAGGAGGATTTTCTCAAAGTGACT 360


32107R-1.IR_full       361 TATAGGGCACATAGACCGTTAGCCGTCGTGCGTGCATATTATCAATATTGGCAGACCAAA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32107R-1.in silico     361 TATAGGGCACATAGACCGTTAGCCGTCGTGCGTGCATATTATCAATATTGGCAGACCAAA 420


32107R-1.IR_full       421 GATCCAAATCTATTGCAATTGGCCTTCGATGAAGATTATTTGCATGCACATCGTCTCTAT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32107R-1.in silico     421 GATCCAAATCTATTGCAATTGGCCTTCGATGAAGATTATTTGCATGCACATCGTCTCTAT 480


32107R-1.IR_full       481 TGCATAGGCTGGTATGATGC 500
                           ||||||||||||||||||||
32107R-1.in silico     481 TGCATAGGCTGGTATGATGC 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  9  NM_168526.1  CG32107-RA (CG32107), mRNA 
0   0  4  5  3  NM_139832.1  CG14829-RA (CG14829), mRNA 
0   0  2  5  7  NM_144343.2  CG8250-RA (Alk), mRNA 
0   0  1  4  5  NM_138136.2  CG30427-RB, transcript variant B (CG30427), mRNA 
0   0  1  3  9  NM_001032053.1  CG33715-RB, transcript variant B (Msp-300), mRNA 
0   0  1  3  9  NM_001032051.1  CG33715-RD, transcript variant D (Msp-300), mRNA 
0   0  1  3  6  NM_136864.2  CG13185-RA (CG13185), mRNA 
0   0  1  3  4  NM_137798.2  CG11061-RA, transcript variant A (GM130), mRNA 
0   0  1  3  4  NM_166513.1  CG11061-RB, transcript variant B (GM130), mRNA 
0   0  1  3  2  NM_079537.2  CG1112-RA, transcript variant A (alpha-Est7), mRNA 
0   0  1  2  2  NM_135615.2  CG6700-RA (CG6700), mRNA 
0   0  1  2  2  NM_167627.1  CG6659-RB, transcript variant B (CG6659), mRNA 
0   0  1  2  2  NM_133089.2  CG6659-RA, transcript variant A (CG6659), mRNA 
0   0  0  7  4  NM_167603.1  CG7178-RF, transcript variant F (wupA), mRNA 
0   0  0  7  4  NM_167608.1  CG7178-RG, transcript variant G (wupA), mRNA 
0   0  0  7  4  NM_078674.2  CG7178-RC, transcript variant C (wupA), mRNA 
0   0  0  5  5  NM_079998.2  CG5912-RA (arr), mRNA 
0   0  0  5  4  NM_135168.3  CG9491-RA (Gef26), mRNA 
0   0  0  4  3  NM_057365.3  CG3182-RA, transcript variant A (sei), mRNA 
0   0  0  4  3  NM_166653.1  CG3182-RB, transcript variant B (sei), mRNA 
0   0  0  3  6  NM_206343.1  CG5620-RB, transcript variant B (CG5620), mRNA 
0   0  0  3  4  NM_168307.1  CG5288-RA, transcript variant A (CG5288), mRNA 
0   0  0  3  4  NM_140019.2  CG5288-RB, transcript variant B (CG5288), mRNA 
0   0  0  3  4  NM_168308.1  CG5288-RC, transcript variant C (CG5288), mRNA 
0   0  0  3  3  NM_166195.1  CG5522-RE, transcript variant E (CG5522), mRNA 
0   0  0  3  3  NM_166196.1  CG5522-RD, transcript variant D (CG5522), mRNA 
0   0  0  3  3  NM_166193.1  CG5522-RA, transcript variant A (CG5522), mRNA 
0   0  0  3  3  NM_165966.1  CG3991-RD, transcript variant D (TppII), mRNA 
0   0  0  3  3  NM_166197.1  CG5522-RF, transcript variant F (CG5522), mRNA 
0   0  0  3  3  NM_137314.2  CG5522-RC, transcript variant C (CG5522), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
+ 
DB
+ 
DC
++ 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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