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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 32092R-3 
 Symbol CG32092  Full Name CG32092 
 CG No CG32092  Old CG No CG32092 
 Synonyms CT18701, CG5956, CG32092 
 Accession No (Link to NCBI) NM_168477.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCCTTTGGCCACCGCATAGAGT 
 Primer Seq. 3' 
aatctagaggtaccCTGTAGCGGTTTGGCAATCGT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CCTTTGGCCA CCGCATAGAG TTGGTGGTGC ACAATTTTAC GCTGGAGGAA GAGTATAGTT 
0061 CCACTGGATG CTGGACAGAC TGGCTGGAAA TAAGGAATGG CGACTCCGAA TCATCTCCGC 
0121 TGATTGGACG CTATTGCGGC AATGAAATTC CAAGCAGGAT TCCCTCATTC GGAAATGTAC 
0181 TGCACCTTAA GTTCAAGTCA GATGACTCCA TGGAGGAGAA GGGATTCCTT TTAAGCTGGC 
0241 AGCAGATGGG AGCCGGATGC GGCGGCAAAT TGAGTTCATC CATGGGGACC ATTCACTCCC 
0301 CGCATTTATT GGCGGGAAAT CGTGGGATCC TTGCTTGCGA CTGGCAGATC ATCGTCGCCG 
0361 AGGGATCCAA AGTAAGCCTG CAGCTGCGGA GCAATGACAA CCGTATATGT AGTGGTCAAC 
0421 TGACCTTATA CGATGGACCC ACCACCGCCA GCAATCCAAT AGTGATTCGT TGCAACGGAA 
0481 CGATTGCCAA ACCGCTACAG  
 in silico PCR Fragment 
0001 CCTTTGGCCA CCGCATAGAG TTGGTGGTGC ACAATTTTAC GCTGGAGGAA GAGTATAGTT 
0061 CCACTGGATG CTGGACAGAC TGGCTGGAAA TAAGGAATGG CGACTCCGAA TCATCTCCGC 
0121 TGATTGGACG CTATTGCGGC AATGAAATTC CAAGCAGGAT TCCCTCATTC GGAAATGTAC 
0181 TGCACCTTAA GTTCAAGTCA GATGACTCCA TGGAGGAGAA GGGATTCCTT TTAAGCTGGC 
0241 AGCAGATGGG AGCCGGATGC GGCGGCAAAT TGAGTTCATC CATGGGAACC ATTCACTCCC 
0301 CGCATTTATT GGCGGGAAAT CGTGGGATCC TTGCTTGCGA CTGGCAGATC ATCGTCGCCG 
0361 AGGGATCCAG AGTAAGCCTG CAGCTGCGGA GCAATGACAA CCGTATATGT AGTGGTCAAC 
0421 TGACCTTATA CGATGGACCC ACCACCGCCA GCAATCCAAT AGTGATTCGT TGCAACGGAA 
0481 CGATTGCCAA ACCGCTACAG  
 Assemble Data 

32092R-3.IR_full       1   CCTTTGGCCACCGCATAGAGTTGGTGGTGCACAATTTTACGCTGGAGGAAGAGTATAGTT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32092R-3.in silico     1   CCTTTGGCCACCGCATAGAGTTGGTGGTGCACAATTTTACGCTGGAGGAAGAGTATAGTT 60


32092R-3.IR_full       61  CCACTGGATGCTGGACAGACTGGCTGGAAATAAGGAATGGCGACTCCGAATCATCTCCGC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32092R-3.in silico     61  CCACTGGATGCTGGACAGACTGGCTGGAAATAAGGAATGGCGACTCCGAATCATCTCCGC 120


32092R-3.IR_full       121 TGATTGGACGCTATTGCGGCAATGAAATTCCAAGCAGGATTCCCTCATTCGGAAATGTAC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32092R-3.in silico     121 TGATTGGACGCTATTGCGGCAATGAAATTCCAAGCAGGATTCCCTCATTCGGAAATGTAC 180


32092R-3.IR_full       181 TGCACCTTAAGTTCAAGTCAGATGACTCCATGGAGGAGAAGGGATTCCTTTTAAGCTGGC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32092R-3.in silico     181 TGCACCTTAAGTTCAAGTCAGATGACTCCATGGAGGAGAAGGGATTCCTTTTAAGCTGGC 240


32092R-3.IR_full       241 AGCAGATGGGAGCCGGATGCGGCGGCAAATTGAGTTCATCCATGGGGACCATTCACTCCC 300
                           |||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
32092R-3.in silico     241 AGCAGATGGGAGCCGGATGCGGCGGCAAATTGAGTTCATCCATGGGAACCATTCACTCCC 300


32092R-3.IR_full       301 CGCATTTATTGGCGGGAAATCGTGGGATCCTTGCTTGCGACTGGCAGATCATCGTCGCCG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32092R-3.in silico     301 CGCATTTATTGGCGGGAAATCGTGGGATCCTTGCTTGCGACTGGCAGATCATCGTCGCCG 360


32092R-3.IR_full       361 AGGGATCCAAAGTAAGCCTGCAGCTGCGGAGCAATGACAACCGTATATGTAGTGGTCAAC 420
                           ||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
32092R-3.in silico     361 AGGGATCCAGAGTAAGCCTGCAGCTGCGGAGCAATGACAACCGTATATGTAGTGGTCAAC 420


32092R-3.IR_full       421 TGACCTTATACGATGGACCCACCACCGCCAGCAATCCAATAGTGATTCGTTGCAACGGAA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32092R-3.in silico     421 TGACCTTATACGATGGACCCACCACCGCCAGCAATCCAATAGTGATTCGTTGCAACGGAA 480


32092R-3.IR_full       481 CGATTGCCAAACCGCTACAG 500
                           ||||||||||||||||||||
32092R-3.in silico     481 CGATTGCCAAACCGCTACAG 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_168477.1  CG32092-RB (CG32092), mRNA 
0.2   1  2  3  5  NM_166559.1  CG3622-RA, transcript variant A (CG3622), mRNA 
0.2   1  2  3  5  NM_137874.2  CG3622-RB, transcript variant B (CG3622), mRNA 
0   0  2  2  3  NM_176370.1  CG16993-RA (in), mRNA 
0   0  1  3  3  NM_057512.3  CG3724-RA (Pgd), mRNA 
0   0  1  3  2  NM_132317.1  CG12121-RA (CG12121), mRNA 
0   0  1  2  5  NM_167768.1  CG32819-RB, transcript variant B (CG32819), mRNA 
0   0  1  2  5  NM_167765.2  CG32820-RB, transcript variant B (CG32820), mRNA 
0   0  1  2  5  NM_167762.2  CG17450-RA, transcript variant A (CG17450), mRNA 
0   0  1  2  3  NM_176003.1  CG13124-RB, transcript variant B (CG13124), mRNA 
0   0  1  2  3  NM_135475.1  CG13124-RA, transcript variant A (CG13124), mRNA 
0   0  1  2  3  NM_143413.1  CG11880-RA, transcript variant A (CG11880), mRNA 
0   0  1  2  3  NM_170389.1  CG11880-RB, transcript variant B (CG11880), mRNA 
0   0  1  2  3  NM_170390.1  CG11880-RC, transcript variant C (CG11880), mRNA 
0   0  1  2  2  NM_166487.2  CG13492-RB, transcript variant B (CG13492), mRNA 
0   0  1  2  2  NM_137768.1  CG13492-RA, transcript variant A (CG13492), mRNA 
0   0  0  6  6  NM_001043189.1  CG40049-RB, transcript variant B (mRpS5), mRNA 
0   0  0  6  5  NM_136107.2  CG17568-RA (CG17568), mRNA 
0   0  0  5  6  NM_165693.2  CG30004-RA (CG30004), mRNA 
0   0  0  5  4  NM_136977.2  CG17575-RA (CG17575), mRNA 
0   0  0  5  4  NM_141381.2  CG15598-RA (Osi17), mRNA 
0   0  0  4  7  NM_132476.2  CG1657-RA (CG1657), mRNA 
0   0  0  4  4  NM_139649.1  CG15023-RA (CG15023), mRNA 
0   0  0  4  3  NM_079747.2  CG5394-RA, transcript variant A (Aats-glupro), mRNA 
0   0  0  4  3  NM_170103.1  CG5394-RB, transcript variant B (Aats-glupro), mRNA 
0   0  0  4  3  NM_137094.1  CG8394-RA (CG8394), mRNA 
0   0  0  4  2  NM_132531.1  CG11802-RA (CG11802), mRNA 
0   0  0  3  25  NM_167193.1  CG32702-RA (CG32702), mRNA 
0   0  0  3  13  NM_140803.1  CG14073-RB, transcript variant B (CG14073), mRNA 
0   0  0  3  10  NM_136864.2  CG13185-RA (CG13185), mRNA 

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