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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
32081R-2 |
| Symbol |
CG32081 |
Full Name |
CG32081 |
| CG No |
CG32081 |
Old CG No |
CG32081 |
| Synonyms |
CG11806, BcDNA:AT27573, CG32081 |
| Accession No (Link to NCBI) |
NM_168430.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGGGCGACAAAGGAGGTTTTGA |
| Primer Seq. 3' |
aatctagaggtaccGTATCTGGAACGGGGGTATAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GGGCGANCAA AGGAGGTTTT GATCCTTATG AAAATCGAAA TGTGGCTCAC CCGATATCTG
0061 ATATTGGGGC GTTTTTTTCG CTTCTTAAAT GTGTCGTTGG AACGGGAGTT ATGGCCATTC
0121 CATTATCCTT TAATTATGCT GGCATCGTCA CTGGCATAAT CCTTCTAGTT TCGGTATGCT
0181 TTATGCTTAT CCATGGAATG CAGATGCTGA TTATTTGCAT GATTGAGTGC TCGCGTCGAA
0241 TGCAAATTGG ATATGCAACT TATCCAGTCG CGATGGTATA CTCATTTGAT CAGGGACCCA
0301 GGTTCTTTAA GTATATCTCA AAGGCCGGAA GATATATAGT CGATGGAGTC TTGGCATTCT
0361 CCCAATTTGG AGTCTGTGTC GTGTACAATG TATTTGTGGC TGCCACCTTG AAGCAACTCG
0421 TCGACTTTTA CTGGGTAGTG GCAGACTTGA GAATCTATAT AGCCGTGATC GCACTCTGCC
0481 TTATACCCCC GTTCCAGATA C |
| in silico PCR Fragment |
0001 GGGCGA-CAA AGGAGGTTTT GATCCTTATG AAAATCGAAA TGTGGCTCAC CCGATATCTG
0061 ATATTGGGGC GTTTTTTTCG CTTCTTAAAT GTGTCGTTGG AACGGGAGTT ATGGCCATTC
0121 CATTATCCTT TAATTATGCT GGCATCGTCA CTGGCATAAT CCTTCTAGTT TCGGTATGCT
0181 TTATGCTTAT CCATGGAATG CAGATGCTGA TTATTTGCAT GATTGAGTGC TCGCGTCGAA
0241 TGCAAATTGG ATATGCAACT TATCCAGTCG CGATGGTATA CTCATTTGAT CAGGGACCCA
0301 GGTTCTTTAA GTATATCTCA AAGGCCGGAA GATATATAGT CGATGGAGTC TTGGCATTCT
0361 CCCAATTTGG AGTCTGTGTC GTGTACAATG TATTTGTGGC TGCCACCTTG AAGCAACTCG
0421 TCGACTTTTA CTGGGTAGTG GCAGACTTGA GAATCTATAT AGCCGTGATC GCACTCTGCC
0481 TTATACCCCC GTTCCAGATA C |
| Assemble Data |
32081R-2.IR_full 1 GGGCGANCAAAGGAGGTTTTGATCCTTATGAAAATCGAAATGTGGCTCACCCGATATCTG 60
|||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
32081R-2.in silico 1 GGGCGA-CAAAGGAGGTTTTGATCCTTATGAAAATCGAAATGTGGCTCACCCGATATCTG 60
32081R-2.IR_full 61 ATATTGGGGCGTTTTTTTCGCTTCTTAAATGTGTCGTTGGAACGGGAGTTATGGCCATTC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32081R-2.in silico 61 ATATTGGGGCGTTTTTTTCGCTTCTTAAATGTGTCGTTGGAACGGGAGTTATGGCCATTC 120
32081R-2.IR_full 121 CATTATCCTTTAATTATGCTGGCATCGTCACTGGCATAATCCTTCTAGTTTCGGTATGCT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32081R-2.in silico 121 CATTATCCTTTAATTATGCTGGCATCGTCACTGGCATAATCCTTCTAGTTTCGGTATGCT 180
32081R-2.IR_full 181 TTATGCTTATCCATGGAATGCAGATGCTGATTATTTGCATGATTGAGTGCTCGCGTCGAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32081R-2.in silico 181 TTATGCTTATCCATGGAATGCAGATGCTGATTATTTGCATGATTGAGTGCTCGCGTCGAA 240
32081R-2.IR_full 241 TGCAAATTGGATATGCAACTTATCCAGTCGCGATGGTATACTCATTTGATCAGGGACCCA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32081R-2.in silico 241 TGCAAATTGGATATGCAACTTATCCAGTCGCGATGGTATACTCATTTGATCAGGGACCCA 300
32081R-2.IR_full 301 GGTTCTTTAAGTATATCTCAAAGGCCGGAAGATATATAGTCGATGGAGTCTTGGCATTCT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32081R-2.in silico 301 GGTTCTTTAAGTATATCTCAAAGGCCGGAAGATATATAGTCGATGGAGTCTTGGCATTCT 360
32081R-2.IR_full 361 CCCAATTTGGAGTCTGTGTCGTGTACAATGTATTTGTGGCTGCCACCTTGAAGCAACTCG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32081R-2.in silico 361 CCCAATTTGGAGTCTGTGTCGTGTACAATGTATTTGTGGCTGCCACCTTGAAGCAACTCG 420
32081R-2.IR_full 421 TCGACTTTTACTGGGTAGTGGCAGACTTGAGAATCTATATAGCCGTGATCGCACTCTGCC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32081R-2.in silico 421 TCGACTTTTACTGGGTAGTGGCAGACTTGAGAATCTATATAGCCGTGATCGCACTCTGCC 480
32081R-2.IR_full 481 TTATACCCCCGTTCCAGATAC 501
|||||||||||||||||||||
32081R-2.in silico 481 TTATACCCCCGTTCCAGATAC 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
1 |
NM_168430.1 |
CG32081-RA (CG32081), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_141701.2 |
CG9459-RA (CG9459), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_132308.1 |
CG12106-RA (CG12106), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_080214.2 |
CG10913-RA (Spn6), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_143530.2 |
CG2217-RA (CG2217), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_079505.2 |
CG1119-RA, transcript variant A (Gnf1), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_001014605.1 |
CG1119-RB, transcript variant B (Gnf1), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_132100.3 |
CG10695-RA (Pat1), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_133143.1 |
CG8002-RA (rictor), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_140327.1 |
CG10522-RA (sti), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_001032104.1 |
CG33911-RA (CG33911), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_138087.3 |
CG13586-RA, transcript variant A (itp), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_143464.2 |
CG1972-RA (CG1972), mRNA |
| 0 |
0 |
0 |
3 |
2 |
NM_080076.2 |
CG11770-RA (lin), mRNA |
| 0 |
0 |
0 |
2 |
7 |
NM_167119.2 |
CG4626-RB, transcript variant B (fz4), mRNA |
| 0 |
0 |
0 |
2 |
7 |
NR_001644.1 |
CG4626-RB, transcript variant B (fz4), mRNA, miscRNA |
| 0 |
0 |
0 |
2 |
7 |
NR_001643.1 |
CG4626-RB, transcript variant B (fz4), mRNA, miscRNA, miscRNA |
| 0 |
0 |
0 |
2 |
7 |
NM_078513.2 |
CG4626-RA, transcript variant A (fz4), mRNA |
| 0 |
0 |
0 |
2 |
6 |
NM_080111.2 |
CG2684-RA (lds), mRNA |
| 0 |
0 |
0 |
2 |
6 |
NM_142451.2 |
CG18600-RA (CG18600), mRNA |
| 0 |
0 |
0 |
2 |
6 |
NM_080118.2 |
CG2075-RA (aly), mRNA |
| 0 |
0 |
0 |
2 |
6 |
NM_138759.2 |
CG9242-RA (bur), mRNA |
| 0 |
0 |
0 |
2 |
5 |
NM_130549.2 |
CG12773-RA (CG12773), mRNA |
| 0 |
0 |
0 |
2 |
5 |
NM_139711.2 |
CG10626-RA (Lkr), mRNA |
| 0 |
0 |
0 |
2 |
5 |
NM_132539.2 |
CG1559-RA (Upf1), mRNA |
| 0 |
0 |
0 |
2 |
4 |
NM_176195.1 |
CG33017-RB (CG33017), mRNA |
| 0 |
0 |
0 |
2 |
4 |
NM_139882.1 |
CG7716-RA (CG7716), mRNA |
| 0 |
0 |
0 |
2 |
4 |
NM_136787.1 |
CG12943-RA (CG12943), mRNA |
| 0 |
0 |
0 |
2 |
4 |
NM_144343.2 |
CG8250-RA (Alk), mRNA |
| 0 |
0 |
0 |
2 |
3 |
NM_058161.3 |
CG5595-RA (Sce), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| ++ |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| ++ |
|
| DB |
|
| + |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| ++ |
|
| VB |
|
| |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
|
|
|
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