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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 31970R-1 
 Symbol CG15630  Full Name CG15630 
 CG No CG15630  Old CG No CG31970 
 Synonyms CT35785, CG31970, CG15630 
 Accession No (Link to NCBI) NM_175961.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTGGCAGCCCTAACAGCTGTCG 
 Primer Seq. 3' 
aatctagaggtaccCAAACCATCGGCCAGGATGCG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TGGCAGCCCT ANACAGCTGT CGCCTGGGCC AACCACCATG AGAGCCTTTC CCTCAGTCCG 
0061 GCGGAGcACA GCGTGGTGCG GNTACACAAA CGAATCCCTC ATCGTCCAGT GCCGCAGTCC 
0121 CGATCCCAAA GTGGAGCTCC ATTGGAAATC GCCGAAAGGC GAAATTATAC GCGAGCACAA 
0181 AGGACGCATT CACATTGAGC AAACATCGAC AGAACAATTG AAAATCGTTT TCGCCCACAT 
0241 CGCACTGGCC GACAAGGGCA ATTGGAGCTG CGAAGCTGCT GATGGAAGTC TGCATAGTAA 
0301 ATCCTTCGAT TTGATTGTGT ACCAGAAAAT TACATTCACG GAAAATGCCA CCGTGATGAC 
0361 GGTCAAGGAG GGCGAGAAGG CCACCATCCT GTGCGAGGTG AAGGGCGAGC CCCAGCCGAA 
0421 TGTCACCTGG CACTTCAATG GACGAGCCCA TCAGTGCCGG CGCGGCCGAC GACTCCAAGT 
0481 TCCGCATCCT GGCCGATGGT TTG 
 in silico PCR Fragment 
0001 TGGCAGCCCT A-ACAGCTGT CGCCTGGGCC AACCACCATG AGAGCCTTTC CCTCAGTCCG 
0061 GCGGAGCACA GCGTGGTGCG G-TACACAAA CGAATCCCTC ATCGTCCAGT GCCGCAGTCC 
0121 CGATCCCAAA GTGGAGCTCC ATTGGAAATC GCCGAAAGGC GAAATTATAC GCGAGCACAA 
0181 AGGACGCATT CACATTGAGC AAACATCGAC AGAACAATTG AAAATCGTTT TCGCCCACAT 
0241 CGCACTGGCC GACAAGGGCA ATTGGAGCTG CGAAGCTGCT GATGGAAGTC TGCATAGTAA 
0301 ATCCTTCGAT TTGATTGTGT ACCAGAAAAT TACATTCACG GAAAATGCCA CCGTGATGAC 
0361 GGTCAAGGAG GGCGAGAAGG CCACCATCCT GTGCGAGGTG AAGGGCGAGC CCCAGCCGAA 
0421 TGTCACCTGG CACTTCAATG GAC-AGCCCA TCAGTGCCGG CGCGGCCGAC GACTCCAAGT 
0481 TCCGCATCCT GGCCGATGGT TTG 
 Assemble Data 

31970R-1.IR_full       1   TGGCAGCCCTANACAGCTGTCGCCTGGGCCAACCACCATGAGAGCCTTTCCCTCAGTCCG 60
                           ||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
31970R-1.in silico     1   TGGCAGCCCTA-ACAGCTGTCGCCTGGGCCAACCACCATGAGAGCCTTTCCCTCAGTCCG 60


31970R-1.IR_full       61  GCGGAGCACAGCGTGGTGCGGNTACACAAACGAATCCCTCATCGTCCAGTGCCGCAGTCC 120
                           ||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
31970R-1.in silico     61  GCGGAGCACAGCGTGGTGCGG-TACACAAACGAATCCCTCATCGTCCAGTGCCGCAGTCC 120


31970R-1.IR_full       121 CGATCCCAAAGTGGAGCTCCATTGGAAATCGCCGAAAGGCGAAATTATACGCGAGCACAA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31970R-1.in silico     121 CGATCCCAAAGTGGAGCTCCATTGGAAATCGCCGAAAGGCGAAATTATACGCGAGCACAA 180


31970R-1.IR_full       181 AGGACGCATTCACATTGAGCAAACATCGACAGAACAATTGAAAATCGTTTTCGCCCACAT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31970R-1.in silico     181 AGGACGCATTCACATTGAGCAAACATCGACAGAACAATTGAAAATCGTTTTCGCCCACAT 240


31970R-1.IR_full       241 CGCACTGGCCGACAAGGGCAATTGGAGCTGCGAAGCTGCTGATGGAAGTCTGCATAGTAA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31970R-1.in silico     241 CGCACTGGCCGACAAGGGCAATTGGAGCTGCGAAGCTGCTGATGGAAGTCTGCATAGTAA 300


31970R-1.IR_full       301 ATCCTTCGATTTGATTGTGTACCAGAAAATTACATTCACGGAAAATGCCACCGTGATGAC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31970R-1.in silico     301 ATCCTTCGATTTGATTGTGTACCAGAAAATTACATTCACGGAAAATGCCACCGTGATGAC 360


31970R-1.IR_full       361 GGTCAAGGAGGGCGAGAAGGCCACCATCCTGTGCGAGGTGAAGGGCGAGCCCCAGCCGAA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31970R-1.in silico     361 GGTCAAGGAGGGCGAGAAGGCCACCATCCTGTGCGAGGTGAAGGGCGAGCCCCAGCCGAA 420


31970R-1.IR_full       421 TGTCACCTGGCACTTCAATGGACGAGCCCATCAGTGCCGGCGCGGCCGACGACTCCAAGT 480
                           ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||
31970R-1.in silico     421 TGTCACCTGGCACTTCAATGGAC-AGCCCATCAGTGCCGGCGCGGCCGACGACTCCAAGT 480


31970R-1.IR_full       481 TCCGCATCCTGGCCGATGGTTTG 503
                           |||||||||||||||||||||||
31970R-1.in silico     481 TCCGCATCCTGGCCGATGGTTTG 503



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_175961.1  CG15630-RA (CG15630), mRNA 
0.2   1  2  5  2  NM_132417.1  CG12637-RA (CG12637), mRNA 
0   0  3  4  2  NM_135547.1  CG5056-RA (CG5056), mRNA 
0   0  2  3  2  NM_130655.1  CG8636-RA (CG8636), mRNA 
0   0  2  2  4  NM_132500.2  CG11699-RA (CG11699), mRNA 
0   0  2  2  4  NM_167777.1  CG14619-RC, transcript variant C (CG14619), mRNA 
0   0  2  2  3  NM_167776.1  CG14619-RE, transcript variant E (CG14619), mRNA 
0   0  2  2  3  NM_167775.1  CG14619-RD, transcript variant D (CG14619), mRNA 
0   0  2  2  3  NM_134618.2  CG14619-RA, transcript variant A (CG14619), mRNA 
0   0  1  6  5  NM_166554.1  CG3612-RA (blw), mRNA 
0   0  1  5  5  NM_001043053.1  CG17800-PAG (Dscam), mRNA 
0   0  1  5  5  NM_001043061.1  CG17800-PAD (Dscam), mRNA 
0   0  1  5  5  NM_001043023.1  CG17800-PAQ (Dscam), mRNA 
0   0  1  5  5  NM_001043025.1  CG17800-PAZ (Dscam), mRNA 
0   0  1  5  5  NM_001043034.1  CG17800-PBA (Dscam), mRNA 
0   0  1  5  4  NM_001043065.1  CG17800-RK, transcript variant K (Dscam), mRNA 
0   0  1  5  4  NM_001043055.1  CG17800-RL, transcript variant L (Dscam), mRNA 
0   0  1  5  4  NM_001043044.1  CG17800-RM, transcript variant M (Dscam), mRNA 
0   0  1  5  4  NM_001043043.1  CG17800-RN, transcript variant N (Dscam), mRNA 
0   0  1  5  4  NM_001043014.1  CG17800-PAL (Dscam), mRNA 
0   0  1  5  4  NM_165513.1  CG17800-RC, transcript variant C (Dscam), mRNA 
0   0  1  5  4  NM_001043033.1  CG17800-RO, transcript variant O (Dscam), mRNA 
0   0  1  5  4  NM_001043027.1  CG17800-PAR (Dscam), mRNA 
0   0  1  5  4  NM_001043031.1  CG17800-RP, transcript variant P (Dscam), mRNA 
0   0  1  5  4  NM_001043012.1  CG17800-PAC (Dscam), mRNA 
0   0  1  5  3  NM_001043047.1  CG17800-RH, transcript variant H (Dscam), mRNA 
0   0  1  5  3  NM_001043019.1  CG17800-PAS (Dscam), mRNA 
0   0  1  5  3  NM_078925.5  CG17800-RD, transcript variant D (Dscam), mRNA 
0   0  1  5  3  NM_001043049.1  CG17800-RF, transcript variant F (Dscam), mRNA 
0   0  1  5  3  NM_001043060.1  CG17800-RG, transcript variant G (Dscam), mRNA 

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