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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
31956R-2 |
| Symbol |
pgant4 |
Full Name |
polypeptide GalNAc transferase 4 |
| CG No |
CG31956 |
Old CG No |
CG31956 |
| Synonyms |
CG31956, CG8845b, CG8845, pgant4 |
| Accession No (Link to NCBI) |
NM_164539.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Yamamoto-Hino M, Yoshida H, Ichimiya T, Sakamura S, Maeda M, Kimura Y, Sasaki N, Aoki-Kinoshita KF, Kinoshita-Toyoda A, Toyoda H, Ueda R, Nishihara S, Goto S.
Phenotype-based clustering of glycosylation-related genes by RNAi-mediated gene silencing.
Genes Cells (2015) 20(6) 521-42
[
PubMed ID = 25940448
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGTCACCACTTTGGTGGTCGAA |
| Primer Seq. 3' |
aatctagaggtaccAGGGTGTTAAAGTGCTCGTTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 NTCACCACTT TGGTgGTCGA ACGGCGAATG AAAAATGCGG CNGAGctGAC GGAACAACTC
0061 GATCCGAATg GAGATCCCAT AACGCCCGTT TTTAGGGCTG CCAATATACA TCCAACTCGG
0121 AAAGCGCCAC GACCACCATT TCAGGATCGC AACTCCGTGG TAGATATTCC GAGAAGTGAC
0181 AAACTCCAGG GATTCCGACT ACCGGAACCG AAGGGAGAGC GCAAGGATTG GCACGACTAT
0241 GCGGCCATGG AGGCGGATAG AAAGCGATCC GGTTTCGGTG AGCACGGAGT AGCCGTAAAG
0301 ATCGAAAATC CCGATGAGAA GCAGTTGGAA AAGGAGCACT ACGAGATGAA CGGCTTTAAT
0361 GGCCTCATAT CGGATAGAAT CTCCGTCAAT CGATCTGTGC CAGACCTCAG ACTCGAGGCT
0421 TGCAAGACCC GGAAGTATCT GGCCAAGTTG CCCAATATCA GCGTGATTTT CATCTTCTTC
0481 AACGAGCACT TTAACACCCT |
| in silico PCR Fragment |
0001 GTCACCACTT TGGTGGTCGA ACGGCGAATG AAAAATGCGG CGGAGCTGAC GGAACAACTC
0061 GATCCGAATG GAGATCCCAT AACGCCCGTT TTTAGGGCTG CCAATATACA TCCAACTCGG
0121 AAAGCGCCAC GACCACCATT TCAGGATCGC AACTCCGTGG TAGATATTCC GAGAAGTGAC
0181 AAACTCCAGG GATTCCGACT ACCGGAACCG AAGGGAGAGC GCAAGGATTG GCACGACTAT
0241 GCGGCCATGG AGGCGGATAG AAAGCGATCC GGTTTCGGTG AGCACGGAGT AGCCGTAAAG
0301 ATCGAAAATC CCGATGAGAA GCAGTTGGAA AAGGAGCACT ACGAGATGAA CGGCTTTAAT
0361 GGCCTCATAT CGGATAGAAT CTCCGTCAAT CGATCTGTGC CAGACCTCAG ACTCGAGGCT
0421 TGCAAGACCC GGAAGTATCT GGCCAAGTTG CCCAATATCA GCGTGATTTT CATCTTCTTC
0481 AACGAGCACT TTAACACCCT |
| Assemble Data |
31956R-2.IR_full 1 TCACCACTTTGGTGGTCGAACGGCGAATGAAAAATGCGGCNGAGCTGACGGAACAACTC 59
|||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
31956R-2.in silico 1 TCACCACTTTGGTGGTCGAACGGCGAATGAAAAATGCGGCGGAGCTGACGGAACAACTC 59
31956R-2.IR_full 61 GATCCGAATGGAGATCCCATAACGCCCGTTTTTAGGGCTGCCAATATACATCCAACTCGG 119
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31956R-2.in silico 61 GATCCGAATGGAGATCCCATAACGCCCGTTTTTAGGGCTGCCAATATACATCCAACTCGG 119
31956R-2.IR_full 121 AAAGCGCCACGACCACCATTTCAGGATCGCAACTCCGTGGTAGATATTCCGAGAAGTGAC 179
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31956R-2.in silico 121 AAAGCGCCACGACCACCATTTCAGGATCGCAACTCCGTGGTAGATATTCCGAGAAGTGAC 179
31956R-2.IR_full 181 AAACTCCAGGGATTCCGACTACCGGAACCGAAGGGAGAGCGCAAGGATTGGCACGACTAT 239
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31956R-2.in silico 181 AAACTCCAGGGATTCCGACTACCGGAACCGAAGGGAGAGCGCAAGGATTGGCACGACTAT 239
31956R-2.IR_full 241 GCGGCCATGGAGGCGGATAGAAAGCGATCCGGTTTCGGTGAGCACGGAGTAGCCGTAAAG 299
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31956R-2.in silico 241 GCGGCCATGGAGGCGGATAGAAAGCGATCCGGTTTCGGTGAGCACGGAGTAGCCGTAAAG 299
31956R-2.IR_full 301 ATCGAAAATCCCGATGAGAAGCAGTTGGAAAAGGAGCACTACGAGATGAACGGCTTTAAT 359
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31956R-2.in silico 301 ATCGAAAATCCCGATGAGAAGCAGTTGGAAAAGGAGCACTACGAGATGAACGGCTTTAAT 359
31956R-2.IR_full 361 GGCCTCATATCGGATAGAATCTCCGTCAATCGATCTGTGCCAGACCTCAGACTCGAGGCT 419
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31956R-2.in silico 361 GGCCTCATATCGGATAGAATCTCCGTCAATCGATCTGTGCCAGACCTCAGACTCGAGGCT 419
31956R-2.IR_full 421 TGCAAGACCCGGAAGTATCTGGCCAAGTTGCCCAATATCAGCGTGATTTTCATCTTCTTC 479
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31956R-2.in silico 421 TGCAAGACCCGGAAGTATCTGGCCAAGTTGCCCAATATCAGCGTGATTTTCATCTTCTTC 479
31956R-2.IR_full 481 AACGAGCACTTTAACACCCT 499
||||||||||||||||||||
31956R-2.in silico 481 AACGAGCACTTTAACACCCT 499
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_164539.1 |
CG31956-RA (pgant4), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_057257.3 |
CG7664-RA (crp), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_169095.1 |
CG1093-RB, transcript variant B (plx), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_169094.1 |
CG1093-RA, transcript variant A (plx), mRNA |
| 0 |
0 |
2 |
7 |
2 |
NM_140836.1 |
CG14082-RA (CG14082), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_167436.1 |
CG6170-RB, transcript variant B (HDAC6), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_132789.2 |
CG6170-RA, transcript variant A (HDAC6), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_167437.1 |
CG6170-RC, transcript variant C (HDAC6), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_130616.2 |
CG4290-RA (CG4290), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_079754.2 |
CG5695-RA, transcript variant A (jar), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001014650.1 |
CG5695-RC, transcript variant C (jar), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_170138.1 |
CG5695-RB, transcript variant B (jar), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001014647.1 |
CG5695-RF, transcript variant F (jar), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001014648.1 |
CG5695-RE, transcript variant E (jar), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001014649.1 |
CG5695-RD, transcript variant D (jar), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_135914.1 |
CG15253-RA (CG15253), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_132660.2 |
CG1716-RA (CG1716), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_134682.2 |
CG4133-RA (CG4133), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_001043010.1 |
CG9432-RD, transcript variant D (l(2)01289), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_165485.1 |
CG9432-RB, transcript variant B (l(2)01289), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_133158.2 |
CG12204-RA (CG12204), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_166059.1 |
CG30076-RA (CG30076), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_205997.1 |
CG15270-RB, transcript variant B (CG15270), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_135894.2 |
CG15270-RA, transcript variant A (CG15270), mRNA |
| 0 |
0 |
0 |
5 |
21 |
NM_144448.2 |
CG1915-RC, transcript variant C (sls), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_137199.2 |
CG8182-RA, transcript variant A (GalNAc-T1), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_166099.1 |
CG8182-RB, transcript variant B (GalNAc-T1), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_206710.1 |
CG1372-RB, transcript variant B (yl), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_078596.2 |
CG1372-RA, transcript variant A (yl), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_165913.1 |
CG8581-RB, transcript variant B (fra), mRNA |
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