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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 31918R-3 
 Symbol CG31918  Full Name CG31918 
 CG No CG31918  Old CG No CG31918 
 Synonyms CG18592, anon-WO0118547.477, CG31918 
 Accession No (Link to NCBI) NM_135060.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Álvarez-Fernández C, Tamirisa S, Prada F, Chernomoretz A, Podhajcer O, Blanco E, Martín-Blanco E.
Identification and functional analysis of healing regulators in Drosophila.
PLoS Genet (2015) 11(2) e1004965 [ PubMed ID = 25647511 ] [ RRC reference ]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCCCAATCTCGAGATCATGAAG 
 Primer Seq. 3' 
aatctagaggtaccGGCAGACCAATATTTTGCAGC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CCCAATCTCG AGATCATGAA GCGAATGCTA GACGACATGG ATGAAAAAGT CGAACCATGT 
0061 CACAATTTCT ACAACTCCTC CCAAAAGAAT CTCAAGTGGC ATGCATTTGC GACGGTGAGC 
0121 GATCAGTTCC AACTCCTCTT CGACGGAATT GAGAACGAGG TGTTCGAGAA GCACAGCTTC 
0181 GAGGAAAAGT TGCAGCGTCT ATACAAAATC TGTTCGACCA TGGAGAGACC CAGTCTTCTG 
0241 AGGTATTTTG AGCTGGTGAA GCCGGGAGAA GATCTCAACT GGCCCTTTCT GACGCCCCGA 
0301 GGAGGCAAGT GGCCCAAGGA GAAGTTTCAG TGGATGGAGG CACTGGNCTC GACTGCGTCG 
0361 GTACGGCTTC AATGACATCA TGTTTGGAAT GAGTATAAGA CAGGATCCGA ACAGTGACAG 
0421 TGGCCAACCC ATAGTAGTTT TTGAAAAAAA GTACGATTCT CTATTCCGTT TCATAGAGTC 
0481 GCTGCAAAAT ATTGGTNCTN Cc 
 in silico PCR Fragment 
0001 CCCAATCTCG AGATCATGAA GCGAATGCTA GACGACATGG ATGAAAAAGT CGAACCATGT 
0061 CACAATTTCT ACAACTCCTC CCAAAAGAAT CTCAAGTGGC ATGCATTTGC GACGGTGAGC 
0121 GATCAGTTCC AACTCCTCTT CGACGGAATT GAGAACGAGG TGTTCGAGAA GCACAGCTTC 
0181 GAGGAAAAGT TGCAGCGTCT ATACAAAATC TGTTCGACCA TGGAGAGACC CAGTCTTCTG 
0241 AGGTATTTTG AGCTGGTGAA GCCGGGAGAA GATCTCAACT GGCCCTTTCT GACGCCCCGA 
0301 GGAGGCAAGT GGCCCAAGGA GAAGTTTCAG TGGATGGAGG CACTGG-CTC GACTGCGTCG 
0361 GTACGGCTTC AATGACATCA TGTTTGGAAT GAGTATAAGA CAGGATCCGA ACAGTGACAG 
0421 TGGCCAACCC ATAGTAGTTT TTGAAAAAAA GTACGATTCT CTATTCCGTT TCATAGAGTC 
0481 GCTGCAAAAT ATTGGT-CTG CC 
 Assemble Data 

31918R-3.IR_full       1   CCCAATCTCGAGATCATGAAGCGAATGCTAGACGACATGGATGAAAAAGTCGAACCATGT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31918R-3.in silico     1   CCCAATCTCGAGATCATGAAGCGAATGCTAGACGACATGGATGAAAAAGTCGAACCATGT 60


31918R-3.IR_full       61  CACAATTTCTACAACTCCTCCCAAAAGAATCTCAAGTGGCATGCATTTGCGACGGTGAGC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31918R-3.in silico     61  CACAATTTCTACAACTCCTCCCAAAAGAATCTCAAGTGGCATGCATTTGCGACGGTGAGC 120


31918R-3.IR_full       121 GATCAGTTCCAACTCCTCTTCGACGGAATTGAGAACGAGGTGTTCGAGAAGCACAGCTTC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31918R-3.in silico     121 GATCAGTTCCAACTCCTCTTCGACGGAATTGAGAACGAGGTGTTCGAGAAGCACAGCTTC 180


31918R-3.IR_full       181 GAGGAAAAGTTGCAGCGTCTATACAAAATCTGTTCGACCATGGAGAGACCCAGTCTTCTG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31918R-3.in silico     181 GAGGAAAAGTTGCAGCGTCTATACAAAATCTGTTCGACCATGGAGAGACCCAGTCTTCTG 240


31918R-3.IR_full       241 AGGTATTTTGAGCTGGTGAAGCCGGGAGAAGATCTCAACTGGCCCTTTCTGACGCCCCGA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31918R-3.in silico     241 AGGTATTTTGAGCTGGTGAAGCCGGGAGAAGATCTCAACTGGCCCTTTCTGACGCCCCGA 300


31918R-3.IR_full       301 GGAGGCAAGTGGCCCAAGGAGAAGTTTCAGTGGATGGAGGCACTGGNCTCGACTGCGTCG 360
                           |||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
31918R-3.in silico     301 GGAGGCAAGTGGCCCAAGGAGAAGTTTCAGTGGATGGAGGCACTGG-CTCGACTGCGTCG 360


31918R-3.IR_full       361 GTACGGCTTCAATGACATCATGTTTGGAATGAGTATAAGACAGGATCCGAACAGTGACAG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31918R-3.in silico     361 GTACGGCTTCAATGACATCATGTTTGGAATGAGTATAAGACAGGATCCGAACAGTGACAG 420


31918R-3.IR_full       421 TGGCCAACCCATAGTAGTTTTTGAAAAAAAGTACGATTCTCTATTCCGTTTCATAGAGTC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31918R-3.in silico     421 TGGCCAACCCATAGTAGTTTTTGAAAAAAAGTACGATTCTCTATTCCGTTTCATAGAGTC 480


31918R-3.IR_full       481 GCTGCAAAATATTGGTNCTNCC 502
                           |||||||||||||||| || ||
31918R-3.in silico     481 GCTGCAAAATATTGGT-CTGCC 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_135060.2  CG31918-RA (CG31918), mRNA 
0   0  2  3  4  NM_142868.1  CG13830-RA (CG13830), mRNA 
0   0  2  2  5  NM_080088.1  CG6577-RA (can), mRNA 
0   0  2  2  3  NM_166211.2  CG30459-RA (CG30459), mRNA 
0   0  1  3  2  NM_141584.3  CG8379-RA, transcript variant A (CG8379), mRNA 
0   0  1  3  2  NM_206463.1  CG8379-RB, transcript variant B (CG8379), mRNA 
0   0  1  3  2  NM_001032059.1  CG10890-RC, transcript variant C (mus201), mRNA 
0   0  1  3  2  NM_001032061.1  CG10890-RA, transcript variant A (mus201), mRNA 
0   0  1  3  2  NM_001032060.1  CG10890-RB, transcript variant B (mus201), mRNA 
0   0  1  3  2  NM_057299.3  CG13399-RA, transcript variant A (Chrac-14), mRNA 
0   0  1  2  6  NM_079928.1  CG17116-RA (Lip2), mRNA 
0   0  1  2  6  NM_135859.1  CG17341-RA (CG17341), mRNA 
0   0  1  2  4  NM_170254.1  CG8384-RA, transcript variant A (gro), mRNA 
0   0  1  2  4  NM_079790.3  CG8384-RD, transcript variant D (gro), mRNA 
0   0  1  2  4  NM_170256.1  CG8384-RC, transcript variant C (gro), mRNA 
0   0  1  2  4  NM_079617.3  CG7855-RA (timeout), mRNA 
0   0  1  2  4  NM_170255.2  CG8384-RB, transcript variant B (gro), mRNA 
0   0  1  2  4  NM_206575.1  CG8384-RE, transcript variant E (gro), mRNA 
0   0  1  2  3  NM_078595.1  CG18319-RA (ben), mRNA 
0   0  1  2  3  NM_138249.2  CG9139-RA (CG9139), mRNA 
0   0  1  2  2  NM_141612.2  CG8420-RA (CG8420), mRNA 
0   0  0  6  6  NM_130656.2  CG9904-RA (CG9904), mRNA 
0   0  0  6  5  NM_136397.1  CG15236-RA, transcript variant A (CG15236), mRNA 
0   0  0  6  5  NM_165493.1  CG15236-RB, transcript variant B (CG15236), mRNA 
0   0  0  5  14  NM_140686.3  CG3764-RA (CG3764), mRNA 
0   0  0  5  2  NM_164929.1  CG6206-RB, transcript variant B (CG6206), mRNA 
0   0  0  5  2  NM_135564.3  CG6206-RA, transcript variant A (CG6206), mRNA 
0   0  0  4  4  NM_001014678.1  CG33553-RE, transcript variant E (Doa), mRNA 
0   0  0  3  18  NM_001043010.1  CG9432-RD, transcript variant D (l(2)01289), mRNA 
0   0  0  3  18  NM_165485.1  CG9432-RB, transcript variant B (l(2)01289), mRNA 

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