NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 31913R-1

Symbol CG31913 Full Name CG31913

CG No CG31913 Old CG No CG31913

Synonyms NEST:bs23g06, NEST:bs28e01, NEST:bs35c08, NEST:bs14h07, NEST:bs09g05, CG31913

Accession No (Link to NCBI) NM_164662.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGGGCAGCATATGCATCTGCG

Primer Seq. 3'

aatctagaggtaccCGGCTCCTGCTCCTTATCTTC

Predicted Fragment Size

IR fragment full Seq

0001 TGGGCAGCAT ATGCATCTGC GGAGCACTCG CCTCCATCTC GGGCCTGGCC TACATGCGCT 
0061 GGCGACTTGA GGACCGTGTA CGCCAGACGG AGTTCTATCA GTTGGCCATC CANGCAGCTA 
0121 CGCCAGCACA GTGGAGCGGT CGGTCTGCTG GGCGAGCCCA TCAAGGAGTC GGGCTTTAAT 
0181 CTGTCCAACG AGAAGAATCG CTGTGACGAG GACAAGGCCC AGCTGCAGTT CCATGTCCAG 
0241 GGTCCCAAGG ACAGGGGGAC CGTCTATTTC TGGGCCTCAA ACAACCAGAA ACAGGGCTGG 
0301 CTGATCGATC GCCTGGAGCT CGAAACGAGA CAGAACCCGA ACACACGCTA CCTGCTTAAG 
0361 AAGCCCCCGA ACTACTCGCT GGTCAGCAGT GATGGTGATC CGGATCCCCC TAATGCCGAG 
0421 TCCTCCGAGG AAACCCAGCA GCCGCAGACG CCGCTCGAGC CACAGGAGCA GATGGAAATG 
0481 GAAGATAAGG AGCAGGAGCC G

in silico PCR Fragment

0001 TGGGCAGCAT ATGCATCTGC GGAGCACTCG CCTCCATCTC GGGCCTGGCC TACATGCGCT 
0061 GGCGACTTGA GGACCGTGTA CGCCAGACGG AGTTCTATCA GTTGGCCATC CA-GCAGCTA 
0121 CGCCAGCACA GTGGAGCGGT CGGTCTGCTG GGCGAGCCCA TCAAGGAGTC GGGCTTTAAT 
0181 CTGTCCAACG AGAAGAATCG CTGTGACGAG GACAAGGCCC AGCTGCAGTT CCATGTCCAG 
0241 GGTCCCAAGG ACAGGGGGAC CGTCTATTTC TGGGCCTCAA ACAACCAGAA ACAGGGCTGG 
0301 CTGATCGATC GCCTGGAGCT CGAAACGAGA CAGAACCCGA ACACACGCTA CCTGCTTAAG 
0361 AAGCCCCCGA ACTACTCGCT GGTCAGCAGT GATGGTGATC CGGATCCCCC TAATGCCGAG 
0421 TCCTCCGAGG AAACCCAGCA GCCGCAGACG CCGCTCGAGC CACAGGAGCA GATGGAAATG 
0481 GAAGATAAGG AGCAGGAGCC G

Assemble Data


31913R-1.IR_full       1   TGGGCAGCATATGCATCTGCGGAGCACTCGCCTCCATCTCGGGCCTGGCCTACATGCGCT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31913R-1.in silico     1   TGGGCAGCATATGCATCTGCGGAGCACTCGCCTCCATCTCGGGCCTGGCCTACATGCGCT 60


31913R-1.IR_full       61  GGCGACTTGAGGACCGTGTACGCCAGACGGAGTTCTATCAGTTGGCCATCCANGCAGCTA 120
                           |||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
31913R-1.in silico     61  GGCGACTTGAGGACCGTGTACGCCAGACGGAGTTCTATCAGTTGGCCATCCA-GCAGCTA 120


31913R-1.IR_full       121 CGCCAGCACAGTGGAGCGGTCGGTCTGCTGGGCGAGCCCATCAAGGAGTCGGGCTTTAAT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31913R-1.in silico     121 CGCCAGCACAGTGGAGCGGTCGGTCTGCTGGGCGAGCCCATCAAGGAGTCGGGCTTTAAT 180


31913R-1.IR_full       181 CTGTCCAACGAGAAGAATCGCTGTGACGAGGACAAGGCCCAGCTGCAGTTCCATGTCCAG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31913R-1.in silico     181 CTGTCCAACGAGAAGAATCGCTGTGACGAGGACAAGGCCCAGCTGCAGTTCCATGTCCAG 240


31913R-1.IR_full       241 GGTCCCAAGGACAGGGGGACCGTCTATTTCTGGGCCTCAAACAACCAGAAACAGGGCTGG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31913R-1.in silico     241 GGTCCCAAGGACAGGGGGACCGTCTATTTCTGGGCCTCAAACAACCAGAAACAGGGCTGG 300


31913R-1.IR_full       301 CTGATCGATCGCCTGGAGCTCGAAACGAGACAGAACCCGAACACACGCTACCTGCTTAAG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31913R-1.in silico     301 CTGATCGATCGCCTGGAGCTCGAAACGAGACAGAACCCGAACACACGCTACCTGCTTAAG 360


31913R-1.IR_full       361 AAGCCCCCGAACTACTCGCTGGTCAGCAGTGATGGTGATCCGGATCCCCCTAATGCCGAG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31913R-1.in silico     361 AAGCCCCCGAACTACTCGCTGGTCAGCAGTGATGGTGATCCGGATCCCCCTAATGCCGAG 420


31913R-1.IR_full       421 TCCTCCGAGGAAACCCAGCAGCCGCAGACGCCGCTCGAGCCACAGGAGCAGATGGAAATG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31913R-1.in silico     421 TCCTCCGAGGAAACCCAGCAGCCGCAGACGCCGCTCGAGCCACAGGAGCAGATGGAAATG 480


31913R-1.IR_full       481 GAAGATAAGGAGCAGGAGCCG 501
                           |||||||||||||||||||||
31913R-1.in silico     481 GAAGATAAGGAGCAGGAGCCG 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_164662.1	CG31913-RA (CG31913), mRNA
0.2	1	3	3	7	NM_176386.1	CG11489-RC, transcript variant C (CG11489), mRNA
0.2	1	2	3	7	NM_143459.1	CG1964-RA (Kul), mRNA
0	0	8	10	22	NM_137696.1	CG15653-RA (CG15653), mRNA
0	0	2	5	4	NM_164655.2	CG7235-RB, transcript variant B (Hsp60C), mRNA
0	0	2	5	4	NM_135104.4	CG7235-RC, transcript variant C (Hsp60C), mRNA
0	0	2	5	4	NM_164654.1	CG7235-RA, transcript variant A (Hsp60C), mRNA
0	0	2	5	2	NM_138013.2	CG16787-RA (CG16787), mRNA
0	0	2	4	5	NM_206761.1	CG33251-RA (CG33251), mRNA
0	0	2	4	3	NM_136583.2	CG8232-RA (CG8232), mRNA
0	0	2	4	2	NM_142268.1	CG6006-RA (CG6006), mRNA
0	0	2	3	4	NM_057883.2	CG10117-RA (ttv), mRNA
0	0	2	3	3	NM_136992.2	CG13325-RA (CG13325), mRNA
0	0	2	2	2	NM_001038753.1	CG11103-RB (CG11103), mRNA
0	0	1	6	3	NM_139389.2	CG12011-RA (CG12011), mRNA
0	0	1	4	53	NM_175960.3	CG33196-RB (dp), mRNA
0	0	1	3	15	NM_001014500.1	CG33558-RA (CG33558), mRNA
0	0	1	3	6	NM_137711.3	CG30389-RA, transcript variant A (CG30389), mRNA
0	0	1	3	6	NM_137712.3	CG30389-RC, transcript variant C (CG30389), mRNA
0	0	1	3	6	NM_166428.2	CG30389-RB, transcript variant B (CG30389), mRNA
0	0	1	3	6	NM_080335.2	CG2984-RA (Pp2C1), mRNA
0	0	1	3	4	NM_167797.1	CG1212-RB, transcript variant B (p130CAS), mRNA
0	0	1	3	4	NM_138158.2	CG1212-RA, transcript variant A (p130CAS), mRNA
0	0	1	3	4	NM_057817.2	CG10637-RA, transcript variant A (Nak), mRNA
0	0	1	3	3	NM_164638.1	CG14025-RC, transcript variant C (Bsg25D), mRNA
0	0	1	3	3	NM_057568.4	CG14025-RA, transcript variant A (Bsg25D), mRNA
0	0	1	3	3	NM_164637.1	CG14025-RB, transcript variant B (Bsg25D), mRNA
0	0	1	3	3	NM_057818.2	CG10637-RB, transcript variant B (Nak), mRNA
0	0	1	3	2	NM_079002.2	CG3905-RA (Su(z)2), mRNA
0	0	1	2	5	NM_206068.1	CG12128-RB, transcript variant B (CG12128), mRNA

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