NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 31900R-7

Symbol CG31900 Full Name CG31900

CG No CG31900 Old CG No CG31900

Synonyms CG31900

Accession No (Link to NCBI) NM_164794.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGCGGAGTTAATGTCACGGAA

Primer Seq. 3'

aatctagaggtaccTTGGTGACCTCAAAGAGTAGC

Predicted Fragment Size

IR fragment full Seq

0001 GNCGNAGTTA ATGTCACGGA AACGTTAAAT AACCAAGAAC TTGATGCCTA CGTACCGCTA 
0061 AACAAGCTGC GACGNGCGTC GATTTTCAAA CTGAAGTTAC AGCCCGAGCT GCACGACAAG 
0121 TACAATGTGT TTGTTCGCGC CGAAAGAAAA GCGGATGTGT TTCTGGGCGA CATCATCAAA 
0181 CGCATCGACA GCTACTACAA AACCGGACAA ACAAAGGCCA AATCAGCCAA ACTTCAGTTT 
0241 CCCGACAAGG AAAAGGACAC GGAAAAGCTA GAGGAAGATG TGGACTCCCC GAAGAACGAA 
0301 ATCGAAACCT TTTCGGAGCA GGAAATCCAA AGTTCTCCTA ATCTGAATCA ANACGCTTTT 
0361 AGCTCCCAGC ACTGGGCAGT CGAGAAGCAC CCTAAATGCC ATGCTTCTCC AGCGATGTGG 
0421 CACAAAAATT GAGTTGGGCA TTGAATCGAA ACTGCTGGTG ATGGCTGGTC AAACAGCTAC 
0481 GCTACTCTTT GAGGTCACCA A

in silico PCR Fragment

0001 GGCGGAGTTA ATGTCACGGA AACGTTAAAT AACCAAGAAC TTGATGCCTA CGTACCGCTA 
0061 AACAAGCTGC GACGCGCGTC GATTTTCAAA CTGAAGTTAC AGCCCGAGCT GCACGACAAG 
0121 TACAATGTGT TTGTTCGCGC CGAAAGAAAA GCGGATGTGT TTCTGGGCGA CATCATCAAA 
0181 CGCATCGACA GCTACTACAA AACCGGACAA ACAAAGGCCA AATCAGCCAA ACTTCAGTTT 
0241 CCCGACAAGG AAAAGGACAC GGAAAAGCTA GAGGAAGATG TGGACTCCCC GAAGAACGAA 
0301 ATCGAAACCT TTTCGGAGCA GGAAATCCAA AGTTCTCCTA ATCTGAATCA A-ACGCTTTT 
0361 AGCTCCCAGC ACTGGGCAGT CGAGAAGCAC CCTAAATGCC ATGCTTCTCC AGCGATGTGG 
0421 CACAAAAATT GAGTTGGGCA TTGAATCGAA ACTGCTGGTG ATGGCTGGTC AAACAGCTAC 
0481 GCTACTCTTT GAGGTCACCA A

Assemble Data


31900R-7.IR_full       1   GNCGNAGTTAATGTCACGGAAACGTTAAATAACCAAGAACTTGATGCCTACGTACCGCTA 60
                           | || |||||||||||||||||||||||||||||||||||||||||||||||||||||||
31900R-7.in silico     1   GGCGGAGTTAATGTCACGGAAACGTTAAATAACCAAGAACTTGATGCCTACGTACCGCTA 60


31900R-7.IR_full       61  AACAAGCTGCGACGNGCGTCGATTTTCAAACTGAAGTTACAGCCCGAGCTGCACGACAAG 120
                           |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
31900R-7.in silico     61  AACAAGCTGCGACGCGCGTCGATTTTCAAACTGAAGTTACAGCCCGAGCTGCACGACAAG 120


31900R-7.IR_full       121 TACAATGTGTTTGTTCGCGCCGAAAGAAAAGCGGATGTGTTTCTGGGCGACATCATCAAA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31900R-7.in silico     121 TACAATGTGTTTGTTCGCGCCGAAAGAAAAGCGGATGTGTTTCTGGGCGACATCATCAAA 180


31900R-7.IR_full       181 CGCATCGACAGCTACTACAAAACCGGACAAACAAAGGCCAAATCAGCCAAACTTCAGTTT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31900R-7.in silico     181 CGCATCGACAGCTACTACAAAACCGGACAAACAAAGGCCAAATCAGCCAAACTTCAGTTT 240


31900R-7.IR_full       241 CCCGACAAGGAAAAGGACACGGAAAAGCTAGAGGAAGATGTGGACTCCCCGAAGAACGAA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31900R-7.in silico     241 CCCGACAAGGAAAAGGACACGGAAAAGCTAGAGGAAGATGTGGACTCCCCGAAGAACGAA 300


31900R-7.IR_full       301 ATCGAAACCTTTTCGGAGCAGGAAATCCAAAGTTCTCCTAATCTGAATCAANACGCTTTT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
31900R-7.in silico     301 ATCGAAACCTTTTCGGAGCAGGAAATCCAAAGTTCTCCTAATCTGAATCAA-ACGCTTTT 360


31900R-7.IR_full       361 AGCTCCCAGCACTGGGCAGTCGAGAAGCACCCTAAATGCCATGCTTCTCCAGCGATGTGG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31900R-7.in silico     361 AGCTCCCAGCACTGGGCAGTCGAGAAGCACCCTAAATGCCATGCTTCTCCAGCGATGTGG 420


31900R-7.IR_full       421 CACAAAAATTGAGTTGGGCATTGAATCGAAACTGCTGGTGATGGCTGGTCAAACAGCTAC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31900R-7.in silico     421 CACAAAAATTGAGTTGGGCATTGAATCGAAACTGCTGGTGATGGCTGGTCAAACAGCTAC 480


31900R-7.IR_full       481 GCTACTCTTTGAGGTCACCAA 501
                           |||||||||||||||||||||
31900R-7.in silico     481 GCTACTCTTTGAGGTCACCAA 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_164794.1	CG31900-RA (CG31900), mRNA
0	0	3	6	4	NM_140431.2	CG6451-RA (blue), mRNA
0	0	3	5	3	NM_170253.2	CG8318-RB, transcript variant B (Nf1), mRNA
0	0	3	5	3	NM_170252.2	CG8318-RC, transcript variant C (Nf1), mRNA
0	0	3	5	3	NM_001014668.1	CG8318-RD, transcript variant D (Nf1), mRNA
0	0	3	3	2	NM_141958.2	CG18616-RA (CG18616), mRNA
0	0	2	3	3	NM_079570.2	CG9434-RA (Fst), mRNA
0	0	2	2	7	NM_080142.2	CG1925-RA (mus205), mRNA
0	0	2	2	2	NM_139458.2	CG1317-RB (CG1317), mRNA
0	0	1	7	12	NM_168179.1	CG32394-RA (CG32394), mRNA
0	0	1	5	5	NM_169138.1	CG31367-RA (CG31367), mRNA
0	0	1	4	2	NM_168841.1	CG32226-RA (CG32226), mRNA
0	0	1	3	5	NM_167395.1	CG32597-RA (l(1)G0469), mRNA
0	0	1	3	3	NM_136672.2	CG1688-RA (CG1688), mRNA
0	0	1	3	2	NM_001015213.1	CG40160-PB.3 (CG40160), mRNA
0	0	1	3	2	NM_001015214.1	CG40160-PC.3 (CG40160), mRNA
0	0	1	3	2	NM_001015212.1	CG40160-PA.3 (CG40160), mRNA
0	0	1	2	7	NM_057638.3	CG14472-RA (poe), mRNA
0	0	1	2	6	NM_130554.2	CG11448-RA (CG11448), mRNA
0	0	1	2	4	NM_135643.2	CG6192-RA (CG6192), mRNA
0	0	1	2	3	NM_001015094.1	CG41040-PA.3 (CG41040), mRNA
0	0	1	2	2	NM_166394.1	CG30142-RA (Obp57b), mRNA
0	0	0	8	6	NM_169197.2	CG31187-RA (CG31187), mRNA
0	0	0	6	11	NM_206265.1	CG12008-RC, transcript variant C (kst), mRNA
0	0	0	6	11	NM_206266.1	CG12008-RB, transcript variant B (kst), mRNA
0	0	0	6	11	NM_079176.1	CG12008-RA, transcript variant A (kst), mRNA
0	0	0	6	11	NM_136069.2	CG10600-RA (CG10600), mRNA
0	0	0	6	9	NM_166186.2	CG18471-RA (gprs), mRNA
0	0	0	6	5	NM_170089.1	CG10367-RA, transcript variant A (Hmgcr), mRNA
0	0	0	6	5	NM_206548.1	CG10367-RB, transcript variant B (Hmgcr), mRNA

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