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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3180R-3 |
| Symbol |
RpII140 |
Full Name |
RNA polymerase II 140kD subunit |
| CG No |
CG3180 |
Old CG No |
CG3180 |
| Synonyms |
pol II, Pol IIc, Pol II, RNAP, wimp, PolII, rpII140/wimp, RNAP II, RNA Pol IIc, H5, polII, RNA Pol II, RP140, RNA pol II, CG3180, RNA polII, DmRP140, unnamed, II, PolIIc, RNApolII, l(3)RplII140, RPII140, RpII140, RpII140[wimp] |
| Accession No (Link to NCBI) |
NM_057358.3 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Park J, Lee J, Kim JH, Lee J, Park H, Lim C.
ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72-associated ALS/FTD.
Nucleic Acids Res (2021) 49(19) 11294-11311
[
PubMed ID = 34551427
]
[
RRC reference
]
Ohhara Y, Nakamura A, Kato Y, Yamakawa-Kobayashi K.
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.
PLoS Genet (2019) 15(4) e1008121
[
PubMed ID = 31034473
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCGAGGAGATCTCTCACGAGC |
| Primer Seq. 3' |
aatctagaggtaccATCCAACGGGCACTCGTTCAG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCGAGGAGAT CTCTCACGAG CTGTGGCAGG AAGCCTGCTG GATCGTGATT AACGCGTACT
0061 TCGACGAGAA GGGCTTGGTG CGGCAGCAGC TGNGACAGTT TCGATGAGTT CATTCAGATG
0121 TCGGTGCAGC GCATTGTGGA GGATTCCCCC GCCATTGAGT TGCAGGCGGA AGCGCAGCAC
0181 ACATCCGGAG AGGTGGAAAC CCCGCCCAGA TTCTCGCTGA AATTCGAGCA AATCTATCTT
0241 TCCAAGCCAA CACATTGGGA AAAGGATGGT TCGCCCAGTC CTATGATGCC GAACGAGGCC
0301 CGCCTGCGAA ATCTAACTTA CTCCGCTCCT CTCTACGTGG ACATTACGAA AACCAAGAAT
0361 GTCGAGGGCC TGGATCCCGT GGAAACGCAG CATCAAAAGA CTTTCATAGG CAAGATTCCC
0421 ATCATGTTGA GATCAACGTA CTGTTTGCTA TCACAGCTTA CTGATCGTGA TCTTACGGAG
0481 CTGAACGAGT GCCCGTTGGA T |
| in silico PCR Fragment |
0001 CCGAGGAGAT CTCTCACGAG CTGTGGCAGG AAGCCTGCTG GATCGTGATT AACGCGTACT
0061 TCGACGAGAA GGGCTTGGTG CGGCAGCAGC TG-GACAGTT TCGATGAGTT CATTCAGATG
0121 TCGGTGCAGC GCATTGTGGA GGATTCCCCC GCCATTGAGT TGCAGGCGGA AGCGCAGCAC
0181 ACATCCGGAG AGGTGGAAAC CCCGCCCAGA TTCTCGCTGA AATTCGAGCA AATCTATCTT
0241 TCCAAGCCAA CACATTGGGA AAAGGATGGT TCGCCCAGTC CTATGATGCC GAACGAGGCC
0301 CGCCTGCGAA ATCTAACTTA CTCCGCTCCT CTCTACGTGG ACATTACGAA AACCAAGAAT
0361 GTCGAGGGCC TGGATCCCGT GGAAACGCAG CATCAAAAGA CTTTCATAGG CAAGATTCCC
0421 ATCATGTTGA GATCAACGTA CTGTTTGCTA TCACAGCTTA CTGATCGTGA TCTTACGGAG
0481 CTGAACGAGT GCCCGTTGGA T |
| Assemble Data |
3180R-3.IR_full 1 CCGAGGAGATCTCTCACGAGCTGTGGCAGGAAGCCTGCTGGATCGTGATTAACGCGTACT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3180R-3.in silico 1 CCGAGGAGATCTCTCACGAGCTGTGGCAGGAAGCCTGCTGGATCGTGATTAACGCGTACT 60
3180R-3.IR_full 61 TCGACGAGAAGGGCTTGGTGCGGCAGCAGCTGNGACAGTTTCGATGAGTTCATTCAGATG 120
|||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
3180R-3.in silico 61 TCGACGAGAAGGGCTTGGTGCGGCAGCAGCTG-GACAGTTTCGATGAGTTCATTCAGATG 120
3180R-3.IR_full 121 TCGGTGCAGCGCATTGTGGAGGATTCCCCCGCCATTGAGTTGCAGGCGGAAGCGCAGCAC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3180R-3.in silico 121 TCGGTGCAGCGCATTGTGGAGGATTCCCCCGCCATTGAGTTGCAGGCGGAAGCGCAGCAC 180
3180R-3.IR_full 181 ACATCCGGAGAGGTGGAAACCCCGCCCAGATTCTCGCTGAAATTCGAGCAAATCTATCTT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3180R-3.in silico 181 ACATCCGGAGAGGTGGAAACCCCGCCCAGATTCTCGCTGAAATTCGAGCAAATCTATCTT 240
3180R-3.IR_full 241 TCCAAGCCAACACATTGGGAAAAGGATGGTTCGCCCAGTCCTATGATGCCGAACGAGGCC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3180R-3.in silico 241 TCCAAGCCAACACATTGGGAAAAGGATGGTTCGCCCAGTCCTATGATGCCGAACGAGGCC 300
3180R-3.IR_full 301 CGCCTGCGAAATCTAACTTACTCCGCTCCTCTCTACGTGGACATTACGAAAACCAAGAAT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3180R-3.in silico 301 CGCCTGCGAAATCTAACTTACTCCGCTCCTCTCTACGTGGACATTACGAAAACCAAGAAT 360
3180R-3.IR_full 361 GTCGAGGGCCTGGATCCCGTGGAAACGCAGCATCAAAAGACTTTCATAGGCAAGATTCCC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3180R-3.in silico 361 GTCGAGGGCCTGGATCCCGTGGAAACGCAGCATCAAAAGACTTTCATAGGCAAGATTCCC 420
3180R-3.IR_full 421 ATCATGTTGAGATCAACGTACTGTTTGCTATCACAGCTTACTGATCGTGATCTTACGGAG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3180R-3.in silico 421 ATCATGTTGAGATCAACGTACTGTTTGCTATCACAGCTTACTGATCGTGATCTTACGGAG 480
3180R-3.IR_full 481 CTGAACGAGTGCCCGTTGGAT 501
|||||||||||||||||||||
3180R-3.in silico 481 CTGAACGAGTGCCCGTTGGAT 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_057358.3 |
CG3180-RA (RpII140), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_169993.1 |
CG31164-RA (CG31164), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_135451.1 |
CG3818-RA (CG3818), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_132843.1 |
CG17209-RA (CG17209), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_137054.1 |
CG6280-RA (CG6280), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_134926.2 |
CG3304-RA, transcript variant A (CG3304), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_205901.1 |
CG3304-RC, transcript variant C (CG3304), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_132786.2 |
CG5877-RA, transcript variant A (CG5877), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_205902.1 |
CG3304-RB, transcript variant B (CG3304), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_133031.1 |
CG6847-RA (CG6847), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_079067.2 |
CG11949-RA, transcript variant A (cora), mRNA |
| 0 |
0 |
0 |
6 |
5 |
NM_142190.2 |
CG4931-RA (Sra-1), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_078686.2 |
CG12230-RA, transcript variant A (car), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_167664.1 |
CG12230-RB, transcript variant B (car), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_001031939.1 |
CG33718-RA, transcript variant A (Pmi), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_001031938.1 |
CG33718-RB, transcript variant B (Pmi), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_137618.1 |
CG9864-RA (CG9864), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_001031940.1 |
CG33717-RC, transcript variant C (PGRP-LD), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_001031941.1 |
CG33717-RB, transcript variant B (PGRP-LD), mRNA |
| 0 |
0 |
0 |
4 |
11 |
NM_140943.2 |
CG32227-RA (CG32227), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_206454.1 |
CG1028-RH, transcript variant H (Antp), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_140417.2 |
CG17364-RA, transcript variant A (CG17364), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_130526.2 |
CG3655-RA (CG3655), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_176328.1 |
CG17364-RB, transcript variant B (CG17364), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_165888.2 |
CG30049-RA (CG30049), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_135037.1 |
CG8871-RA (Jon25Biii), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_131998.2 |
CG3239-RA, transcript variant A (CG3239), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_167038.1 |
CG3239-RB, transcript variant B (CG3239), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_206667.1 |
CG10701-RG, transcript variant G (Moe), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_206668.1 |
CG10701-RF, transcript variant F (Moe), mRNA |
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