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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 31803R-1 
 Symbol CG31803  Full Name CG31803 
 CG No CG31803  Old CG No CG31803 
 Synonyms BcDNA:AT29119, CG31803 
 Accession No (Link to NCBI) NM_165215.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCCTCGACTGCCTAATGTACTG 
 Primer Seq. 3' 
aatctagaggtaccTCTGGTTGGGTATAACACGAC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CCTCGACTGC CTAATGTACT GCGGCATGAA GCTGTCGCCG GAGCAGCGCA TCTTGATTGA 
0061 AAACTCGCTG ATCGCTTTAC AAAATGACAA TCGTTTTACG GGCATGTATC TTTGGGGTCG 
0121 CATTACGGCC ACCAAGAACG ATTACTACAT TGCCTTTGGC TACACTAATG ACTGTCTCAA 
0181 GGATCGCAAG TATTTCTACA GTCTGGACCA GTTCCAATGG CAGTTGCTGC CGTTCGTACA 
0241 GAGCCCCAAG ATATTTCAGG CGACTATATT GGCAAGGGAG CCATTTATAG GAGATCCCAG 
0301 CCTGATGACC TACGTTAAGC TGGACCCCAC TTTTGACATC GTAGGCAATC AGGTGGCTGG 
0361 TATCAGCCGC CCTGAAGTGG TCAAGCTTAA GGAGGAGGAA CGGCTGGCAG CCATTGTGTT 
0421 TATTATAACA GAGGAGTGTG CCATTTGCCC ACGCGGAGCC TTTTACAAGA TGACCGATGG 
0481 TCGTGTTATA CCCAACCAGA  
 in silico PCR Fragment 
0001 CCTCGACTGC CTAATGTACT GCGGCATGAA GCTGTCGCCG GAGCAGCGCA TCTTGATTGA 
0061 AAACTCGCTG ATCGCTTTAC AAAATGACAA TCGTTTTACG GGCATGTATC TTTGGGGTCG 
0121 CATTACGGCC ACCAAGAACG ATTACTACAT TGCCTTTGGC TACACTAATG ACTGTCTCAA 
0181 GGATCGCAAG TATTTCTACA GTCTGGACCA GTTCCAATGG CAGTTGCTGC CGTTCGTACA 
0241 GAGCCCCAAG ATATTTCAGG CGACTATATT GGCAAGGGAG CCATTTATAG GAGATCCCAG 
0301 CCTGATGACC TACGTTAAGC TGGACCCCAC TTTTGACATC GTAGGCAATC AGGTGGCTGG 
0361 TATCAGCCGC CCTGAAGTGG TCAAGCTTAA GGAGGAGGAA CGGCTGGCAG CCATTGTGTT 
0421 TATTATAACA GAGGAGTGTG CCATTTGCCC ACGCGGAGCC TTTTACAAGA TGACCGATGG 
0481 TCGTGTTATA CCCAACCAGA  
 Assemble Data 

31803R-1.IR_full       1   CCTCGACTGCCTAATGTACTGCGGCATGAAGCTGTCGCCGGAGCAGCGCATCTTGATTGA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31803R-1.in silico     1   CCTCGACTGCCTAATGTACTGCGGCATGAAGCTGTCGCCGGAGCAGCGCATCTTGATTGA 60


31803R-1.IR_full       61  AAACTCGCTGATCGCTTTACAAAATGACAATCGTTTTACGGGCATGTATCTTTGGGGTCG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31803R-1.in silico     61  AAACTCGCTGATCGCTTTACAAAATGACAATCGTTTTACGGGCATGTATCTTTGGGGTCG 120


31803R-1.IR_full       121 CATTACGGCCACCAAGAACGATTACTACATTGCCTTTGGCTACACTAATGACTGTCTCAA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31803R-1.in silico     121 CATTACGGCCACCAAGAACGATTACTACATTGCCTTTGGCTACACTAATGACTGTCTCAA 180


31803R-1.IR_full       181 GGATCGCAAGTATTTCTACAGTCTGGACCAGTTCCAATGGCAGTTGCTGCCGTTCGTACA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31803R-1.in silico     181 GGATCGCAAGTATTTCTACAGTCTGGACCAGTTCCAATGGCAGTTGCTGCCGTTCGTACA 240


31803R-1.IR_full       241 GAGCCCCAAGATATTTCAGGCGACTATATTGGCAAGGGAGCCATTTATAGGAGATCCCAG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31803R-1.in silico     241 GAGCCCCAAGATATTTCAGGCGACTATATTGGCAAGGGAGCCATTTATAGGAGATCCCAG 300


31803R-1.IR_full       301 CCTGATGACCTACGTTAAGCTGGACCCCACTTTTGACATCGTAGGCAATCAGGTGGCTGG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31803R-1.in silico     301 CCTGATGACCTACGTTAAGCTGGACCCCACTTTTGACATCGTAGGCAATCAGGTGGCTGG 360


31803R-1.IR_full       361 TATCAGCCGCCCTGAAGTGGTCAAGCTTAAGGAGGAGGAACGGCTGGCAGCCATTGTGTT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31803R-1.in silico     361 TATCAGCCGCCCTGAAGTGGTCAAGCTTAAGGAGGAGGAACGGCTGGCAGCCATTGTGTT 420


31803R-1.IR_full       421 TATTATAACAGAGGAGTGTGCCATTTGCCCACGCGGAGCCTTTTACAAGATGACCGATGG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31803R-1.in silico     421 TATTATAACAGAGGAGTGTGCCATTTGCCCACGCGGAGCCTTTTACAAGATGACCGATGG 480


31803R-1.IR_full       481 TCGTGTTATACCCAACCAGA 500
                           ||||||||||||||||||||
31803R-1.in silico     481 TCGTGTTATACCCAACCAGA 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_165215.1  CG31803-RA (CG31803), mRNA 
0.2   1  2  2  6  NM_170586.1  CG1483-RB, transcript variant B (Map205), mRNA 
0.2   1  2  2  6  NM_079876.2  CG1483-RA, transcript variant A (Map205), mRNA 
0   0  4  2  2  NM_144381.1  CG15378-RA (lectin-22C), mRNA 
0   0  3  2  4  NM_136237.2  CG9265-RA (CG9265), mRNA 
0   0  2  3  2  NM_168458.1  CG32090-RA (Vha16-3), mRNA 
0   0  2  2  5  NM_137058.2  CG12295-RB (stj), mRNA 
0   0  2  2  4  NM_140059.2  CG3448-RB (CG3448), mRNA 
0   0  1  3  4  NM_078692.2  CG11937-RA (amn), mRNA 
0   0  1  2  7  NM_135768.2  CG16972-RA (CG16972), mRNA 
0   0  1  2  4  NM_135517.2  CG4912-RB, transcript variant B (eEF1delta), mRNA 
0   0  1  2  4  NM_164895.1  CG4912-RA, transcript variant A (eEF1delta), mRNA 
0   0  1  2  3  NM_143027.2  CG5805-RA (CG5805), mRNA 
0   0  1  2  2  NM_165352.2  CG9339-RC, transcript variant C (CG9339), mRNA 
0   0  1  2  2  NM_165351.2  CG9339-RF, transcript variant F (CG9339), mRNA 
0   0  1  2  2  NM_165490.1  CG3427-RA (Epac), mRNA 
0   0  0  8  4  NM_143346.1  CG5527-RA (CG5527), mRNA 
0   0  0  6  3  NM_132259.2  CG11219-RA (PIP82), mRNA 
0   0  0  5  3  NM_130508.1  CG14635-RA (CG14635), mRNA 
0   0  0  5  3  NM_140108.1  CG10809-RA (CG10809), mRNA 
0   0  0  5  3  NM_168080.1  CG32251-RA (CG32251), mRNA 
0   0  0  5  2  NM_143558.1  CG2267-RA (CG2267), mRNA 
0   0  0  4  4  NM_001032005.1  CG6782-RD, transcript variant D (CG6782), mRNA 
0   0  0  4  4  NM_001032008.1  CG6783-RC, transcript variant C (CG6783), mRNA 
0   0  0  4  3  NM_167558.1  CG32562-RA (xmas-2), mRNA 
0   0  0  4  3  NM_142805.3  CG6921-RB, transcript variant B (CG6921), mRNA 
0   0  0  4  3  NM_170022.1  CG6921-RA, transcript variant A (CG6921), mRNA 
0   0  0  4  3  NM_170023.1  CG6921-RC, transcript variant C (CG6921), mRNA 
0   0  0  4  2  NM_001032004.1  CG6782-RE, transcript variant E (CG6782), mRNA 
0   0  0  4  2  NM_001032007.1  CG6782-RA, transcript variant A (CG6782), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
+- 
DC
+ 
DM
+- 
VM
 
VB
 
Caspase-3 signal
N
++ 
DB
+ 
DC
++ 
DM
+ 
VM
 
VB
 
Dcompartment   Penetrance
     

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