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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 31800R-1 
 Symbol CG31800  Full Name CG31800 
 CG No CG31800  Old CG No CG31800 
 Synonyms CG31800 
 Accession No (Link to NCBI) NM_165272.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCGAATTGACGTATCAGACAGC 
 Primer Seq. 3' 
aatctagaggtaccGCCCACCATCTAGTGCCTTTA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CGAATTGACG TATCAGACAG CTGTAAAAAA GGCCCAACTG GTTGAGAGGA ATCCCCAGTT 
0061 GCAGATAAAC CCCATGCGTG TGTCCATGCA CCAGGAGGAG GATATCATCG AATTGGCCCA 
0121 ACAAATTCAG AATGCTGATA AACAATTGAA AAACACAACT TGCCAAAAGC TGGGGGTCAT 
0181 AATGGAGCAG ATCAAAATGC TTCAGGCCCA AGCGATGGAG ATACTTAAGG AGTCCACTCT 
0241 CAACCGAGAT CTGCACAGTG CGGCCTGCAA TTTCACCAAA AAACCCGGAC ACATCTATCA 
0301 TCTTTATCAA AGGCCATCGG GTCAAAACTA TTTCAGCATG CTATCACCGG AAGAATGGAG 
0361 TCTCTCCGTT GACCAAACAT TCAAAGGCAG CTACAGGTTG GAGTACGATT TGAGCTGGAC 
0421 CCCACTGGAC AAGATTAAGG AGCAGGATGA GAAGCTAAAG TGGGCGGAGC AGTGCATGCT 
0481 AAAGGCACTA GATGGTGGGC  
 in silico PCR Fragment 
0001 CGAATTGACG TATCAGACAG CTGTAAAAAA GGCCCAACTG GTTGAGAGGA ATCCCCAGTT 
0061 GCAGATAAAC CCCATGCGTG TGTCCATGCA CCAGGAGGAG GATATCATCG AATTGGCCCA 
0121 ACAAATTCAG AATGCTGATA AACAATTGAA AAACACAACT TGCCAAAAGC TGGGGGTCAT 
0181 AATGGAGCAG ATCAAAATGC TTCAGGCCCA AGCGATGGAG ATACTTAAGG AGTCCACTCT 
0241 CAACCGAGAT CTGCACAGTG CGGCCTGCAA TTTCACCAAA AAACCCGGAC ACATCTATCA 
0301 TCTTTATCAA AGGCCATCGG GTCAAAACTA TTTCAGCATG CTATCACCGG AAGAATGGAG 
0361 TCTCTCCGTT GACCAAACAT TCAAAGGCAG CTACAGGTTG GAGTACGATT TGAGCTGGAC 
0421 CCCACTGGAC AAGATTAAGG AGCAGGATGA GAAGCTAAAG TGGGCGGAGC AGTGCATGCT 
0481 AAAGGCACTA GATGGTGGGC  
 Assemble Data 

31800R-1.IR_full       1   CGAATTGACGTATCAGACAGCTGTAAAAAAGGCCCAACTGGTTGAGAGGAATCCCCAGTT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31800R-1.in silico     1   CGAATTGACGTATCAGACAGCTGTAAAAAAGGCCCAACTGGTTGAGAGGAATCCCCAGTT 60


31800R-1.IR_full       61  GCAGATAAACCCCATGCGTGTGTCCATGCACCAGGAGGAGGATATCATCGAATTGGCCCA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31800R-1.in silico     61  GCAGATAAACCCCATGCGTGTGTCCATGCACCAGGAGGAGGATATCATCGAATTGGCCCA 120


31800R-1.IR_full       121 ACAAATTCAGAATGCTGATAAACAATTGAAAAACACAACTTGCCAAAAGCTGGGGGTCAT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31800R-1.in silico     121 ACAAATTCAGAATGCTGATAAACAATTGAAAAACACAACTTGCCAAAAGCTGGGGGTCAT 180


31800R-1.IR_full       181 AATGGAGCAGATCAAAATGCTTCAGGCCCAAGCGATGGAGATACTTAAGGAGTCCACTCT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31800R-1.in silico     181 AATGGAGCAGATCAAAATGCTTCAGGCCCAAGCGATGGAGATACTTAAGGAGTCCACTCT 240


31800R-1.IR_full       241 CAACCGAGATCTGCACAGTGCGGCCTGCAATTTCACCAAAAAACCCGGACACATCTATCA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31800R-1.in silico     241 CAACCGAGATCTGCACAGTGCGGCCTGCAATTTCACCAAAAAACCCGGACACATCTATCA 300


31800R-1.IR_full       301 TCTTTATCAAAGGCCATCGGGTCAAAACTATTTCAGCATGCTATCACCGGAAGAATGGAG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31800R-1.in silico     301 TCTTTATCAAAGGCCATCGGGTCAAAACTATTTCAGCATGCTATCACCGGAAGAATGGAG 360


31800R-1.IR_full       361 TCTCTCCGTTGACCAAACATTCAAAGGCAGCTACAGGTTGGAGTACGATTTGAGCTGGAC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31800R-1.in silico     361 TCTCTCCGTTGACCAAACATTCAAAGGCAGCTACAGGTTGGAGTACGATTTGAGCTGGAC 420


31800R-1.IR_full       421 CCCACTGGACAAGATTAAGGAGCAGGATGAGAAGCTAAAGTGGGCGGAGCAGTGCATGCT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31800R-1.in silico     421 CCCACTGGACAAGATTAAGGAGCAGGATGAGAAGCTAAAGTGGGCGGAGCAGTGCATGCT 480


31800R-1.IR_full       481 AAAGGCACTAGATGGTGGGC 500
                           ||||||||||||||||||||
31800R-1.in silico     481 AAAGGCACTAGATGGTGGGC 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_165272.1  CG31800-RA (CG31800), mRNA 
0   0  3  3  5  NM_130594.1  CG14803-RA (CG14803), mRNA 
0   0  2  5  5  NM_206493.1  CG33485-RA (dpr9), mRNA 
0   0  1  4  12  NM_144448.2  CG1915-RC, transcript variant C (sls), mRNA 
0   0  1  4  4  NM_079937.2  CG1915-RA, transcript variant A (sls), mRNA 
0   0  1  3  4  NM_176244.1  CG9696-RE, transcript variant E (dom), mRNA 
0   0  1  3  4  NM_166446.1  CG9696-RD, transcript variant D (dom), mRNA 
0   0  1  3  4  NM_080094.2  CG9696-RA, transcript variant A (dom), mRNA 
0   0  1  2  6  NM_078600.2  CG10521-RA (NetB), mRNA 
0   0  1  2  6  NM_134591.2  CG1501-RA (unc), mRNA 
0   0  1  2  3  NM_137462.3  CG5721-RA (CG5721), mRNA 
0   0  1  2  2  NR_001944.1  CR32385, miscRNA 
0   0  1  2  2  NM_205995.2  CG33309-RA, transcript variant A (CG33309), mRNA 
0   0  1  2  2  NM_001038819.1  CG33309-RB, transcript variant B (CG33309), mRNA 
0   0  1  2  2  NM_135569.2  CG7456-RA (CG7456), mRNA 
0   0  0  7  14  NM_137943.2  CG3493-RA (CG3493), mRNA 
0   0  0  6  5  NM_142942.3  CG31140-RA, transcript variant A (CG31140), mRNA 
0   0  0  6  5  NM_206553.1  CG31140-RB, transcript variant B (CG31140), mRNA 
0   0  0  6  5  NM_206552.1  CG31140-RC, transcript variant C (CG31140), mRNA 
0   0  0  5  4  NM_057299.3  CG13399-RA, transcript variant A (Chrac-14), mRNA 
0   0  0  5  4  NM_001032060.1  CG10890-RB, transcript variant B (mus201), mRNA 
0   0  0  5  4  NM_001032061.1  CG10890-RA, transcript variant A (mus201), mRNA 
0   0  0  5  4  NM_001032059.1  CG10890-RC, transcript variant C (mus201), mRNA 
0   0  0  5  3  NM_135216.2  CG11070-RA (CG11070), mRNA 
0   0  0  5  3  NM_135507.2  CG18145-RA (Ripalpha), mRNA 
0   0  0  4  7  NM_079741.2  CG10210-RA (tst), mRNA 
0   0  0  4  4  NM_138194.2  CG17090-RA, transcript variant A (CG17090), mRNA 
0   0  0  4  4  NM_167834.1  CG17090-RB, transcript variant B (CG17090), mRNA 
0   0  0  3  18  NM_166019.1  CG18076-RH, transcript variant H (shot), mRNA 
0   0  0  3  15  NM_166015.1  CG18076-RG, transcript variant G (shot), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
+- 
DC
++ 
DM
+- 
VM
 
VB
 
Caspase-3 signal
N
 
DB
+ 
DC
++ 
DM
+ 
VM
 
VB
 
Dcompartment   Penetrance
     

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