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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
31717R-1 |
| Symbol |
CG31717 |
Full Name |
CG31717 |
| CG No |
CG31717 |
Old CG No |
CG31717 |
| Synonyms |
Css2, BcDNA:AT30094, CG31717 |
| Accession No (Link to NCBI) |
NM_164902.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Swarup S, Pradhan-Sundd T, Verheyen EM.
Genome-wide identification of phospho-regulators of Wnt signaling in Drosophila.
Development (2015) 142(8) 1502-15
[
PubMed ID = 25852200
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAGGACGTCATCACCAGCACT |
| Primer Seq. 3' |
aatctagaggtaccCACCGGCGCAGATATCCAAAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GAGGACGTCA TCACCAGCAC TTAAATACCT ATTGGAGAAG GATGTTCAGG TGTCGAAGCA
0061 TTTTGTAGTA GCCGTCTCCA ATCTGTCGTT CTTCAAATCC CTGCGGATCC ACAGCAGATT
0121 TCTGGAGATA TCATGCGATG GAATCGCCTG GTTTGTCTCA TGGATCGCCT TCATCTGGTT
0181 GCTCAGCTCG AAGAGCTTGT ATCAAATGCA AGTCAATATG CTCTTCGGCC TGATACTCGA
0241 CGTAGTGATC GTGGCCGTGC TGAAGGCGCT TGTTCGTCGC AGGCGACCGG TGGCCAGCAA
0301 GGATATGCTG ACCATTGGAC CCGATAAGTT CAGCTTTCCC TCAGGTCATG CCTCCCGGGC
0361 GTTCTTTGTG CTGCTGTTCT TCGCCAAGCT GTATCCGCTT CACATCATAT TCCTAATGCC
0421 GGTCACAGCT TGGGCTGTAA GCGTGGCCAT CTCACGGCTC ATCCTCCAGC GTCATTATAT
0481 TTTGGATATC tGCGCCGGTG |
| in silico PCR Fragment |
0001 GAGGACGTCA TCACCAGCAC TTAAATACCT ATTGGAGAAG GATGTTCAGG TGTCGAAGCA
0061 TTTTGTAGTA GCCGTCTCCA ATCTGTCGTT CTTCAAATCC CTGCGGATCC ACAGCAGATT
0121 TCTGGAGATA TCATGCGATG GAATCGCCTG GTTTGTCTCA TGGATCGCCT TCATCTGGTT
0181 GCTCAGCTCG AAGAGCTTGT ATCAAATGCA AGTCAATATG CTCTTCGGCC TGATACTCGA
0241 CGTAGTGATC GTGGCCGTGC TGAAGGCGCT TGTTCGTCGC AGGCGACCGG TGGCCAGCAA
0301 GGATATGCTG ACCATTGGAC CCGATAAGTT CAGCTTTCCC TCAGGTCATG CCTCCCGGGC
0361 GTTCTTTGTG CTGCTGTTCT TCGCCAAGCT GTATCCGCTT CACATCATAT TCCTAATGCC
0421 GGTCACAGCT TGGGCTGTAA GCGTGGCCAT CTCACGGCTC ATCCTCCAGC GTCATTATAT
0481 TTTGGATATC TGCGCCGGTG |
| Assemble Data |
31717R-1.IR_full 1 GAGGACGTCATCACCAGCACTTAAATACCTATTGGAGAAGGATGTTCAGGTGTCGAAGCA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31717R-1.in silico 1 GAGGACGTCATCACCAGCACTTAAATACCTATTGGAGAAGGATGTTCAGGTGTCGAAGCA 60
31717R-1.IR_full 61 TTTTGTAGTAGCCGTCTCCAATCTGTCGTTCTTCAAATCCCTGCGGATCCACAGCAGATT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31717R-1.in silico 61 TTTTGTAGTAGCCGTCTCCAATCTGTCGTTCTTCAAATCCCTGCGGATCCACAGCAGATT 120
31717R-1.IR_full 121 TCTGGAGATATCATGCGATGGAATCGCCTGGTTTGTCTCATGGATCGCCTTCATCTGGTT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31717R-1.in silico 121 TCTGGAGATATCATGCGATGGAATCGCCTGGTTTGTCTCATGGATCGCCTTCATCTGGTT 180
31717R-1.IR_full 181 GCTCAGCTCGAAGAGCTTGTATCAAATGCAAGTCAATATGCTCTTCGGCCTGATACTCGA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31717R-1.in silico 181 GCTCAGCTCGAAGAGCTTGTATCAAATGCAAGTCAATATGCTCTTCGGCCTGATACTCGA 240
31717R-1.IR_full 241 CGTAGTGATCGTGGCCGTGCTGAAGGCGCTTGTTCGTCGCAGGCGACCGGTGGCCAGCAA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31717R-1.in silico 241 CGTAGTGATCGTGGCCGTGCTGAAGGCGCTTGTTCGTCGCAGGCGACCGGTGGCCAGCAA 300
31717R-1.IR_full 301 GGATATGCTGACCATTGGACCCGATAAGTTCAGCTTTCCCTCAGGTCATGCCTCCCGGGC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31717R-1.in silico 301 GGATATGCTGACCATTGGACCCGATAAGTTCAGCTTTCCCTCAGGTCATGCCTCCCGGGC 360
31717R-1.IR_full 361 GTTCTTTGTGCTGCTGTTCTTCGCCAAGCTGTATCCGCTTCACATCATATTCCTAATGCC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31717R-1.in silico 361 GTTCTTTGTGCTGCTGTTCTTCGCCAAGCTGTATCCGCTTCACATCATATTCCTAATGCC 420
31717R-1.IR_full 421 GGTCACAGCTTGGGCTGTAAGCGTGGCCATCTCACGGCTCATCCTCCAGCGTCATTATAT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31717R-1.in silico 421 GGTCACAGCTTGGGCTGTAAGCGTGGCCATCTCACGGCTCATCCTCCAGCGTCATTATAT 480
31717R-1.IR_full 481 TTTGGATATCTGCGCCGGTG 500
||||||||||||||||||||
31717R-1.in silico 481 TTTGGATATCTGCGCCGGTG 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_164902.1 |
CG31717-RA (CG31717), mRNA |
| 0 |
0 |
5 |
2 |
7 |
NM_134820.2 |
CG10882-RA (CG10882), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_138990.2 |
CG17176-RA (ACXA), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_167289.1 |
CG1572-RB, transcript variant B (CG1572), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_132498.1 |
CG1572-RA, transcript variant A (CG1572), mRNA |
| 0 |
0 |
1 |
7 |
5 |
NM_137571.1 |
CG9416-RA (CG9416), mRNA |
| 0 |
0 |
1 |
6 |
9 |
NM_137832.1 |
CG4554-RA (CG4554), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_079979.2 |
CG18497-RB, transcript variant B (spen), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_164374.1 |
CG18497-RA, transcript variant A (spen), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_079148.2 |
CG6883-RA, transcript variant A (trh), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_001043110.1 |
CG6883-RB, transcript variant B (trh), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_164375.1 |
CG18497-RC, transcript variant C (spen), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_141522.1 |
CG11672-RA (CG11672), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_137751.3 |
CG10082-RB, transcript variant B (CG10082), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_140624.2 |
CG4893-RA (CG4893), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_166466.2 |
CG10082-RA, transcript variant A (CG10082), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_144399.1 |
CG18765-RA (CG18765), mRNA |
| 0 |
0 |
0 |
6 |
6 |
NM_079304.2 |
CG11621-RA, transcript variant A (Pi3K68D), mRNA |
| 0 |
0 |
0 |
6 |
6 |
NM_168475.1 |
CG11621-RC, transcript variant C (Pi3K68D), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_132412.1 |
CG9817-RA (CG9817), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_001038754.1 |
CG12481-RB (CG12481), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_143320.2 |
CG18437-RA (CG18437), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_130604.2 |
CG17766-RA (CG17766), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_140917.1 |
CG14186-RA (CG14186), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_165949.1 |
CG8657-RA (Dgkepsilon), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_135189.2 |
CG9536-RA (CG9536), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_137212.2 |
CG30089-RA (CG30089), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_142646.1 |
CG5483-RA (CG5483), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_164758.1 |
CG6976-RB, transcript variant B (Myo28B1), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_164759.1 |
CG6976-RC, transcript variant C (Myo28B1), mRNA |
|
| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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| Dcompartment |
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Penetrance |
| + |
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