NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 31710R-2

Symbol CG31710 Full Name CG31710

CG No CG31710 Old CG No CG31710

Synonyms CG31710

Accession No (Link to NCBI) NM_164867.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGCTCACCAGACAACCCGAC

Primer Seq. 3'

aatctagaggtaccGTTTAAATCGCAGCTCGCCGC

Predicted Fragment Size

IR fragment full Seq

0001 ATGCTCACCA GACAACCCGA CTACACCATC TCCGAGCTGG GCCATCCCAT CCATCAGTGG 
0061 TCGGATTCGA CCGCGTGCGA GTCCCTCAAG AAGCTGGACT ACACAAAGAT GCCCAGTTCT 
0121 CCGCCATCAC CACATCGCCT GCTGCCACTG AGGGAAACGT TGGACGATTG TTTCAAGCGC 
0181 TTGACGATGA GGAGCAGAGA AGATTTCGAA GAGGATCCCG TATACCAGCA GAAATTGAGG 
0241 AAAAATCAGT GCAATGAACA GCAGCGAAGG CGCAGTATCA AAAATGTCGA GATAAAGCCC 
0301 AGTCCGGAGC AACAACGACG ACGACGACGA CGACAACGAT AGCGGCGAGC TGCGATTTAA 
0361 AC

in silico PCR Fragment

0001 ATGCTCACCA GACAACCCGA CTACACCATC TCCGAGCTGG GCCATCCCAT CCATCAGTGG 
0061 TCGGATTCGA CCGCGTGCGA GTCCCTCAAG AAGCTGGACT ACACAAAGAT GCCCAGTTCT 
0121 CCGCCATCAC CACATCGCCT GCTGCCACTG AGGGAAACGT TGGACGATTG TTTCAAGCGC 
0181 TTGACGATGA GGAGCAGAGA AGATTTCGAA GAGGATCCCG TATACCAGCA GAAATTGAGA 
0241 AAGAATCAGT GCAATGAACA GCAGCGAAGG CGCA--ATCA AAAATGTCGA GATAAAGCCC 
0301 AGTCCGGAGC AACAACGACG ACGACGACGA CGACAACGAT AGCGGCGAGC TGCGATTTAA 
0361 AC

Assemble Data


31710R-2.IR_full       1   ATGCTCACCAGACAACCCGACTACACCATCTCCGAGCTGGGCCATCCCATCCATCAGTGG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31710R-2.in silico     1   ATGCTCACCAGACAACCCGACTACACCATCTCCGAGCTGGGCCATCCCATCCATCAGTGG 60


31710R-2.IR_full       61  TCGGATTCGACCGCGTGCGAGTCCCTCAAGAAGCTGGACTACACAAAGATGCCCAGTTCT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31710R-2.in silico     61  TCGGATTCGACCGCGTGCGAGTCCCTCAAGAAGCTGGACTACACAAAGATGCCCAGTTCT 120


31710R-2.IR_full       121 CCGCCATCACCACATCGCCTGCTGCCACTGAGGGAAACGTTGGACGATTGTTTCAAGCGC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31710R-2.in silico     121 CCGCCATCACCACATCGCCTGCTGCCACTGAGGGAAACGTTGGACGATTGTTTCAAGCGC 180


31710R-2.IR_full       181 TTGACGATGAGGAGCAGAGAAGATTTCGAAGAGGATCCCGTATACCAGCAGAAATTGAGG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| 
31710R-2.in silico     181 TTGACGATGAGGAGCAGAGAAGATTTCGAAGAGGATCCCGTATACCAGCAGAAATTGAGA 240


31710R-2.IR_full       241 AAAAATCAGTGCAATGAACAGCAGCGAAGGCGCAGTATCAAAAATGTCGAGATAAAGCCC 300
                           || |||||||||||||||||||||||||||||||  ||||||||||||||||||||||||
31710R-2.in silico     241 AAGAATCAGTGCAATGAACAGCAGCGAAGGCGCA--ATCAAAAATGTCGAGATAAAGCCC 300


31710R-2.IR_full       301 AGTCCGGAGCAACAACGACGACGACGACGACGACAACGATAGCGGCGAGCTGCGATTTAA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31710R-2.in silico     301 AGTCCGGAGCAACAACGACGACGACGACGACGACAACGATAGCGGCGAGCTGCGATTTAA 360


31710R-2.IR_full       361 AC 362
                           ||
31710R-2.in silico     361 AC 362

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	342	14	24	24	NM_164867.1	CG31710-RA (CG31710), mRNA
2.63	9	38	27	27	NM_167167.1	CG32710-RA (CG32710), mRNA
2.63	9	25	24	24	NM_132437.1	CG15207-RA (CG15207), mRNA
1.46	5	26	28	31	NM_131987.2	CG4202-RA (Sas10), mRNA
1.16	4	27	38	57	NM_001014454.1	CG33526-RA, transcript variant A (PNUTS), mRNA
1.16	4	27	38	57	NM_001014452.1	CG33526-RC, transcript variant C (PNUTS), mRNA
1.16	4	27	38	57	NM_001014455.1	CG33526-RB, transcript variant B (PNUTS), mRNA
1.16	4	19	22	28	NM_001014453.1	CG33526-RD, transcript variant D (PNUTS), mRNA
0.87	3	12	16	20	NM_079067.2	CG11949-RA, transcript variant A (cora), mRNA
0.87	3	12	16	20	NM_166338.1	CG11949-RD, transcript variant D (cora), mRNA
0.87	3	12	16	20	NM_166336.1	CG11949-RC, transcript variant C (cora), mRNA
0.87	3	12	16	20	NM_166337.1	CG11949-RB, transcript variant B (cora), mRNA
0.87	3	6	16	19	NM_078591.2	CG15749-RA (dmrt11E), mRNA
0.58	2	14	19	28	NM_132569.1	CG11245-RA (CG11245), mRNA
0.58	2	14	13	20	NM_142598.1	CG17190-RA (CG17190), mRNA
0.29	1	11	16	21	NM_134717.2	CG4415-RA (CG4415), mRNA
0.29	1	10	23	29	NM_141852.1	CG12594-RA (CG12594), mRNA
0.29	1	9	15	26	NM_132095.2	CG3918-RA (CG3918), mRNA
0.29	1	9	15	24	NM_079842.2	CG17958-RA (Sry-delta), mRNA
0.29	1	8	37	78	NM_001014642.1	CG33518-RA (mun), mRNA
0.29	1	6	28	53	NM_132858.2	CG9056-RA (CG9056), mRNA
0.29	1	3	13	43	NM_136397.1	CG15236-RA, transcript variant A (CG15236), mRNA
0.29	1	3	13	43	NM_165493.1	CG15236-RB, transcript variant B (CG15236), mRNA
0.29	1	3	9	17	NM_001042883.1	CG18405-RC, transcript variant C (Sema-1a), mRNA
0.29	1	3	9	17	NM_135399.2	CG18405-RB, transcript variant B (Sema-1a), mRNA
0.29	1	3	9	17	NM_164830.3	CG18405-RA, transcript variant A (Sema-1a), mRNA
0	0	12	13	20	NM_135694.2	CG6785-RA (CG6785), mRNA
0	0	11	27	58	NM_141465.2	CG14598-RA (CG14598), mRNA
0	0	11	13	20	NM_135412.1	CG9487-RA (CG9487), mRNA
0	0	9	23	24	NM_169783.1	CG31243-RB, transcript variant B (cpo), mRNA

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