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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 31702R-1 
 Symbol CG31702  Full Name CG31702 
 CG No CG31702  Old CG No CG31702 
 Synonyms CG31702 
 Accession No (Link to NCBI) NM_165405.1 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGGATGAGCGCTTACGGACA 
 Primer Seq. 3' 
aatctagaggtaccAGGGTCAGATTGGTACGCTTA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ATGGATGAGC GCTTACGGAC AAGACGTAAG ATCGCAAATA AACTGGAGTC TGGTGGCAAA 
0061 ACAGCACATG CTTTTGAGCG ATTATTGGCA ATTCGCACCA TTAAATTGAG CAAGGGAAAA 
0121 TGTTCCGAAG ACAGTGCAGA GCAGACGAAG TCTAGTGATG ATTGCAAGGC ACAAACGCGT 
0181 AAGGATGTAA ATATCGAGGT CAAAGAGACT TATTCCGGCG ATTCCTCGCC TAACAGTGAG 
0241 AGCGAGAATA CAGCCCAAAA CGATCCGCAA ACGAACGTTG AAAGCGATGA ACGCGTATCC 
0301 AACCTGGAAC AGCTGAGTCG AGCAGTGTTA AAACGGAACG ACATAAAGAA CCTACTGGGT 
0361 AAACCTATTT TCGCGGAGGC TGTGATCGGA TCCTTTGTGC GGCTAAATGT TGGAAAAGTC 
0421 TACTCCATCT ACGAAACTAT TGCTCTGCAT CAGGACAGTA AGGACTACCG GGTAGACGGT 
0481 AAGCGTACCA ATCTGACCCT  
 in silico PCR Fragment 
0001 ATGGATGAGC GCTTACGGAC AAGACGTAAG ATCGCAAATA AACTGGAGTC TGGTGGCAAA 
0061 ACAGCACATG CTTTTGAGCG ATTATTGGCA ATTCGCACCA TTAAATTGAG CAAGGGAAAA 
0121 TGTTCCGAAG ACAGTGCAGA GCAGACGAAG TCTAGTGATG ATTGCAAGGC ACAAACGCGT 
0181 AAGGATGTAA ATATCGAGGT CAAAGAGACT TATTCCGGCG ATTCCTCGCC TAACAGTGAG 
0241 AGCGAGAATA CAGCCCAAAA CGATCCGCAA ATGAACGTAG AAAGCGAAGA ACGCGTATCC 
0301 AACCTGGAAC AGCTGAGTCG AGCAGTGTTA AAACGGAACG ACATAAAGAA CTTACTGGGT 
0361 AAACCTATTT TCGCGGAGGC TGTGATCGGA TCCTTTGTGC GGATAAATGT TGGAAAAGTC 
0421 TACTCCATCT ACGAAACTAT TGCTCTGCAT CAGGACAGTA AGGACTACCG GGTAGACGGT 
0481 AAGCGTACCA ATCTGACCCT  
 Assemble Data 

31702R-1.IR_full       1   ATGGATGAGCGCTTACGGACAAGACGTAAGATCGCAAATAAACTGGAGTCTGGTGGCAAA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31702R-1.in silico     1   ATGGATGAGCGCTTACGGACAAGACGTAAGATCGCAAATAAACTGGAGTCTGGTGGCAAA 60


31702R-1.IR_full       61  ACAGCACATGCTTTTGAGCGATTATTGGCAATTCGCACCATTAAATTGAGCAAGGGAAAA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31702R-1.in silico     61  ACAGCACATGCTTTTGAGCGATTATTGGCAATTCGCACCATTAAATTGAGCAAGGGAAAA 120


31702R-1.IR_full       121 TGTTCCGAAGACAGTGCAGAGCAGACGAAGTCTAGTGATGATTGCAAGGCACAAACGCGT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31702R-1.in silico     121 TGTTCCGAAGACAGTGCAGAGCAGACGAAGTCTAGTGATGATTGCAAGGCACAAACGCGT 180


31702R-1.IR_full       181 AAGGATGTAAATATCGAGGTCAAAGAGACTTATTCCGGCGATTCCTCGCCTAACAGTGAG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31702R-1.in silico     181 AAGGATGTAAATATCGAGGTCAAAGAGACTTATTCCGGCGATTCCTCGCCTAACAGTGAG 240


31702R-1.IR_full       241 AGCGAGAATACAGCCCAAAACGATCCGCAAACGAACGTTGAAAGCGATGAACGCGTATCC 300
                           ||||||||||||||||||||||||||||||| |||||| |||||||| ||||||||||||
31702R-1.in silico     241 AGCGAGAATACAGCCCAAAACGATCCGCAAATGAACGTAGAAAGCGAAGAACGCGTATCC 300


31702R-1.IR_full       301 AACCTGGAACAGCTGAGTCGAGCAGTGTTAAAACGGAACGACATAAAGAACCTACTGGGT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
31702R-1.in silico     301 AACCTGGAACAGCTGAGTCGAGCAGTGTTAAAACGGAACGACATAAAGAACTTACTGGGT 360


31702R-1.IR_full       361 AAACCTATTTTCGCGGAGGCTGTGATCGGATCCTTTGTGCGGCTAAATGTTGGAAAAGTC 420
                           |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
31702R-1.in silico     361 AAACCTATTTTCGCGGAGGCTGTGATCGGATCCTTTGTGCGGATAAATGTTGGAAAAGTC 420


31702R-1.IR_full       421 TACTCCATCTACGAAACTATTGCTCTGCATCAGGACAGTAAGGACTACCGGGTAGACGGT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31702R-1.in silico     421 TACTCCATCTACGAAACTATTGCTCTGCATCAGGACAGTAAGGACTACCGGGTAGACGGT 480


31702R-1.IR_full       481 AAGCGTACCAATCTGACCCT 500
                           ||||||||||||||||||||
31702R-1.in silico     481 AAGCGTACCAATCTGACCCT 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_165405.1  CG31702-RA (CG31702), mRNA 
91.28   440  42  0  0  NM_001042918.1  CG31703-RB, transcript variant B (CG31703), mRNA 
91.28   440  42  0  0  NM_165407.1  CG31703-RA, transcript variant A (CG31703), mRNA 
80.08   386  54  20  4  NM_165404.1  CG31700-RA (CG31700), mRNA 
0   0  2  4  3  NM_138025.2  CG3121-RA (CG3121), mRNA 
0   0  2  2  3  NM_133079.2  CG6481-RA (CG6481), mRNA 
0   0  1  4  2  NM_078548.2  CG2985-RA (Yp1), mRNA 
0   0  1  2  2  NM_136387.1  CG9430-RA (CG9430), mRNA 
0   0  0  4  7  NM_079853.2  CG15532-RA, transcript variant A (hdc), mRNA 
0   0  0  4  7  NM_176591.1  CG15532-RC, transcript variant C (hdc), mRNA 
0   0  0  4  2  NM_137704.2  CG9394-RA (CG9394), mRNA 
0   0  0  4  2  NM_165515.1  CG17800-RB, transcript variant B (Dscam), mRNA 
0   0  0  4  2  NM_165514.1  CG17800-RA, transcript variant A (Dscam), mRNA 
0   0  0  4  2  NM_165513.1  CG17800-RC, transcript variant C (Dscam), mRNA 
0   0  0  4  2  NM_001043057.1  CG17800-PAM (Dscam), mRNA 
0   0  0  4  2  NM_001043020.1  CG17800-PBB (Dscam), mRNA 
0   0  0  4  2  NM_078925.5  CG17800-RD, transcript variant D (Dscam), mRNA 
0   0  0  3  9  NM_176462.1  CG31116-RD, transcript variant D (CG31116), mRNA 
0   0  0  3  5  NM_136477.2  CG30497-RA, transcript variant A (CG30497), mRNA 
0   0  0  3  5  NM_165555.1  CG30497-RB, transcript variant B (CG30497), mRNA 
0   0  0  3  5  NM_133024.2  CG6769-RA (CG6769), mRNA 
0   0  0  3  5  NM_168516.1  CG17667-RB, transcript variant B (CG17667), mRNA 
0   0  0  3  5  NM_140343.2  CG17667-RA, transcript variant A (CG17667), mRNA 
0   0  0  3  4  NM_143794.2  CG7129-RA, transcript variant A (l(3)05822), mRNA 
0   0  0  3  4  NM_169802.1  CG7129-RB, transcript variant B (l(3)05822), mRNA 
0   0  0  3  4  NM_141400.2  CG10267-RA (CG10267), mRNA 
0   0  0  3  4  NM_140673.3  CG32171-RB, transcript variant B (Lmpt), mRNA 
0   0  0  3  4  NM_206388.1  CG32171-RG, transcript variant G (Lmpt), mRNA 
0   0  0  3  3  NM_001014644.2  CG7956-RB, transcript variant B (CG7956), mRNA 
0   0  0  3  3  NM_142715.2  CG7956-RA, transcript variant A (CG7956), mRNA 

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