|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
3166R-3 |
| Symbol |
aop |
Full Name |
anterior open |
| CG No |
CG3166 |
Old CG No |
CG3166 |
| Synonyms |
Yan, AOP, YAN, yan, Aop, CG3166, Aop/Yan, DROYANETSB, SK2-1, unnamed, DROYANET, aop/yan, pok, yan/pok, aop |
| Accession No (Link to NCBI) |
NM_164457.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Duncan OF, Granat L, Ranganathan R, Singh VK, Mazaud D, Fanto M, Chambers D, Ballard CG, Bateman JM.
Ras-ERK-ETS inhibition alleviates neuronal mitochondrial dysfunction by reprogramming mitochondrial retrograde signaling.
PLoS Genet (2018) 14(7) e1007567
[
PubMed ID = 30059502
]
[
RRC reference
]
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Fairchild MJ, Islam F, Tanentzapf G.
Identification of genetic networks that act in the somatic cells of the testis to mediate the developmental program of spermatogenesis.
PLoS Genet (2017) 13(9) e1007026
[
PubMed ID = 28957323
]
[
RRC reference
]
Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244
[
PubMed ID = 27908785
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTCATTGAACTCGCTGAATCCC |
| Primer Seq. 3' |
aatctagaggtaccACCGAGTTAGGCGCCATAAAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TCATTGAACT CGCTGAATCC CGGCATCTGG AGCGATGTGC TGTTGCGCTG TCCCCCAGCG
0061 CCGTCCAGCC AGCTGGCGGA GCTTAAGACG CAACTGCCCC CATCCCTGCC TTCCGATCCG
0121 CGCCTCTGGA GCCGCGAGGA TGTGCTTGTC NTTCCTGCGG TTCTGCGTCC GCGAATTCGA
0181 TCTCCCCAAA CTCGACTTTG ATCTCTTCCA AATGAACGGC AAGNGCGCTG TGCTTGCTGA
0241 CACGGGCGGA CTTTGGTCAC CGGTGTCCTG GGGCAGGAGA TGTGCTCCAC AATGTGCTGC
0301 AGATGTTGAT CATAGAGTCC CACATGATGC AGTGGCACCT GCCTAACAGT CCAGTGACGC
0361 CCACCAGTCG TTATCCCCTG TCGCCGCACA GCCATCCGCC GACGCCCACG TGGCCACCGC
0421 TGAATGCTCC TCCCGAGAAC AGTCCCTTCC ACAGTTCTGC TCACAGTCTG GCAGGCCACC
0481 ATTTTATGGC GCCTAACTCG GT |
| in silico PCR Fragment |
0001 TCATTGAACT CGCTGAATCC CGGCATCTGG AGCGATGTGC TGTGGCGCTG TCCCCCAGCG
0061 CCGTCCAGCC AGCTGGCGGA GCTTAAGACG CAACTGCCCC CATCCCTGCC TTCCGATCCG
0121 CGCCTCTGGA GCCGCGAGGA TGTGCTTGTC -TTCCTGCGG TTCTGCGTCC GCGAATTCGA
0181 TCTCCCCAAA CTTGACTTTG ATCTCTTCCA AATGAACGGC AAG-GCGCTG TGCTTGCTGA
0241 CGCGGGCGGA CTTTGGTCAC CGGTGTCCTG GGGCAGGAGA TGTGCTCCAC AATGTGCTGC
0301 AGATGTTGAT CATAGAGTCC CACATGATGC AGTGGCACCT GCCTAATAGT CCAGTGACGC
0361 CCACCAGTCG TTATCCCCTG TCGCCGCACA GCCATCCGCC GACGCCCACG TGGCCACCGC
0421 TGAATGCTCC TCCCGAGAAC AGTCCCTTCC ACAGTTCTGC TCACAGTCTG GCAGGCCACC
0481 ATTTTATGGC GCCTAACTCG GT |
| Assemble Data |
3166R-3.IR_full 1 TCATTGAACTCGCTGAATCCCGGCATCTGGAGCGATGTGCTGTTGCGCTGTCCCCCAGCG 60
||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
3166R-3.in silico 1 TCATTGAACTCGCTGAATCCCGGCATCTGGAGCGATGTGCTGTGGCGCTGTCCCCCAGCG 60
3166R-3.IR_full 61 CCGTCCAGCCAGCTGGCGGAGCTTAAGACGCAACTGCCCCCATCCCTGCCTTCCGATCCG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3166R-3.in silico 61 CCGTCCAGCCAGCTGGCGGAGCTTAAGACGCAACTGCCCCCATCCCTGCCTTCCGATCCG 120
3166R-3.IR_full 121 CGCCTCTGGAGCCGCGAGGATGTGCTTGTCNTTCCTGCGGTTCTGCGTCCGCGAATTCGA 180
|||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
3166R-3.in silico 121 CGCCTCTGGAGCCGCGAGGATGTGCTTGTC-TTCCTGCGGTTCTGCGTCCGCGAATTCGA 180
3166R-3.IR_full 181 TCTCCCCAAACTCGACTTTGATCTCTTCCAAATGAACGGCAAGNGCGCTGTGCTTGCTGA 240
|||||||||||| |||||||||||||||||||||||||||||| ||||||||||||||||
3166R-3.in silico 181 TCTCCCCAAACTTGACTTTGATCTCTTCCAAATGAACGGCAAG-GCGCTGTGCTTGCTGA 240
3166R-3.IR_full 241 CACGGGCGGACTTTGGTCACCGGTGTCCTGGGGCAGGAGATGTGCTCCACAATGTGCTGC 300
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3166R-3.in silico 241 CGCGGGCGGACTTTGGTCACCGGTGTCCTGGGGCAGGAGATGTGCTCCACAATGTGCTGC 300
3166R-3.IR_full 301 AGATGTTGATCATAGAGTCCCACATGATGCAGTGGCACCTGCCTAACAGTCCAGTGACGC 360
|||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
3166R-3.in silico 301 AGATGTTGATCATAGAGTCCCACATGATGCAGTGGCACCTGCCTAATAGTCCAGTGACGC 360
3166R-3.IR_full 361 CCACCAGTCGTTATCCCCTGTCGCCGCACAGCCATCCGCCGACGCCCACGTGGCCACCGC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3166R-3.in silico 361 CCACCAGTCGTTATCCCCTGTCGCCGCACAGCCATCCGCCGACGCCCACGTGGCCACCGC 420
3166R-3.IR_full 421 TGAATGCTCCTCCCGAGAACAGTCCCTTCCACAGTTCTGCTCACAGTCTGGCAGGCCACC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3166R-3.in silico 421 TGAATGCTCCTCCCGAGAACAGTCCCTTCCACAGTTCTGCTCACAGTCTGGCAGGCCACC 480
3166R-3.IR_full 481 ATTTTATGGCGCCTAACTCGGT 502
||||||||||||||||||||||
3166R-3.in silico 481 ATTTTATGGCGCCTAACTCGGT 502
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_078731.2 |
CG3166-RB, transcript variant B (aop), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_164457.1 |
CG3166-RA, transcript variant A (aop), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_166202.2 |
CG6657-RA, transcript variant A (veg), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_079946.3 |
CG6657-RB, transcript variant B (veg), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_130519.2 |
CG3699-RA (CG3699), mRNA |
| 0 |
0 |
2 |
3 |
7 |
NM_137595.1 |
CG11025-RA, transcript variant A (isopeptidase-T-3), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_141696.1 |
CG8526-RA (CG8526), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_169060.1 |
CG8526-RA (CG8526), mRNA,4,5,-tris-phosphate receptor CG1063-RB, transcript variant B (Itp-r83A), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_169061.1 |
CG8526-RA (CG8526), mRNA,4,5,-tris-phosphate receptor CG1063-RB, transcript variant B (Itp-r83A), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_143270.2 |
CG6265-RA, transcript variant A (Nep5), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_170307.1 |
CG6265-RB, transcript variant B (Nep5), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_141364.1 |
CG1148-RB, transcript variant B (Osi2), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_169130.1 |
CG1148-RA, transcript variant A (Osi2), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_136767.2 |
CG12936-RA (CG12936), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_001043247.1 |
CG6535-RB (tefu), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_133162.1 |
CG12231-RA (CG12231), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_169449.2 |
CG14736-RC, transcript variant C (CG14736), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_165552.2 |
CG18812-RC, transcript variant C (CG18812), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_169448.1 |
CG14736-RA, transcript variant A (CG14736), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_169447.1 |
CG14736-RB, transcript variant B (CG14736), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_080011.2 |
CG7035-RB, transcript variant B (Cbp80), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_167012.1 |
CG7035-RA, transcript variant A (Cbp80), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_137816.1 |
CG5819-RA, transcript variant A (CG5819), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_166523.1 |
CG5819-RB, transcript variant B (CG5819), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_140674.2 |
CG11915-RA (CG11915), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_141682.2 |
CG8412-RA (CG8412), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_139395.1 |
CG12035-RA (CG12035), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_079062.2 |
CG15085-RA (edl), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_143372.1 |
CG9995-RA (htt), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_170158.1 |
CG5794-RB, transcript variant B (CG5794), mRNA |
|
|
|
